The Effect of Extract/Fractions of Caralluma tuberculata on Blood Glucose Levels and Body Weight in Alloxan-Induced Diabetic Rabbits

2014 ◽  
Vol 19 (3) ◽  
pp. 195-199 ◽  
Author(s):  
Khushbakht Sultan ◽  
Muhammad Zakir ◽  
Haroon Khan ◽  
Ihsaan Ullah Khan ◽  
Ali Rehman ◽  
...  
Keyword(s):  
Body Weight ◽  
Blood Glucose ◽  
Ethyl Acetate Fraction ◽  
The Body ◽  
Antidiabetic Activity ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
Cooked Food ◽  
Diabetic Rabbits ◽  
Positive Effect

Caralluma tuberculata is a cooked food item in Pakistan especially for diabetics. The current study was designed to explore the antidiabetic potential of extract/fractions of Caralluma tuberculata in alloxan-induced diabetic rabbits and its effect on body weight. The crude extract of the plant provoked 24% and 44% antidiabetic action at 25 and 50 mg/kg OP, respectively, after the 24th day of treatment, which was strongly supported by a positive effect on the body weight of animals. On fractionation, pretreatment of the ethyl acetate fraction demonstrated most dominant (25.17% and 34.83%) antidiabetic activity followed by n-hexane (19.33% and 32.76%) and aqueous fractions (16.44% and 22.36%) at 25 and 50 mg/kg OP, respectively, after the 24th day of treatment. The corresponding effect on blood glucose was also observed on body weight of diabetic rabbits. In sum, extract/fractions of the plant showed marked antidiabetic action and thus a provided scientific foundation to the use of the plant as an antidiabetic.

scholarly journals Screening of achillea santolina for anti-diabetic activity and its comparison with caralluma tuberculata.

2020 ◽  
Vol 27 (07) ◽  
pp. 1414-1419
Author(s):  
Muhammad Saleh Faisal ◽  
Asad Inayat ◽  
Muhammad Nabi ◽  
Waqar Hayat ◽  
Muhammad Sajid Khan ◽  
...  
Keyword(s):  
Body Weight ◽  
Blood Glucose ◽  
Crude Extract ◽  
Glucose Levels ◽  
Cooking Oil ◽  
Blood Glucose Levels ◽  
Crude Extracts ◽  
Diabetic Rabbits ◽  
Medical University ◽  
Mean Blood Glucose

Objectives: The study was designed to investigate the hypoglycaemic effect of Achillea santolina and its comparison with Caralluma tuberculata. Study Design: Experimental study. Setting: Khyber Medical University, Peshawar. Period: July 2017 to February 2018. Material & Methods: Crude extract and carbon tetrachloride (CCl4) extract of Achillea santolina were prepared and administered to normal and alloxan treated diabetic rabbits. These extracts were given in capsule form as well as in cooking oil and their effects on blood glucose levels were observed at 0, 2, 4, 6, 8, 12 and 24 hours of administration. The results were compared with Caralluma tuberculata. Results: The crude extract 200 mg/kg body weight of Achillea santolina showed marked reduction in mean blood glucose level at 2, 4, 8 and 12 hours with significant (p<0.001) difference when compared with Caralluma tuberculata. The metformin 500 mg/kg body weight was also given to compare its effects with the plant crude extracts, which was more or less intermediate between the crude extracts of Achillea santolina and Caralluma tuberculata. The diabetic rabbits were treated with 100 mg/kg body weight CCl4 fraction of Achillea in capsule form. Blood glucose levels dropped markedly at 2 hours and further more at 4 hours as observed with Caralluma. Upon comparison with metformin, Achillea found to lower blood glucose levels but quite late than metformin. The diabetic rabbits were also treated with 100 mg/kg body weight CCl4 fraction of Achillea santolina in cooking oil and Caralluma was found to cause higher reduction in blood glucose levels than Achillea. Conclusion: Both Achillea santolina and Caralluma tuberculata lowered the blood glucose levels but the later turned out to be more significant in developing hypoglycaemia, particularly when taken with cooking oil.

scholarly journals Study of Blood Glucose and SGPT lowering effect of Eucalyptus Lanceolata Linn. in Induced Diabetic Rabbits

2015 ◽  
pp. 152-158
Author(s):  
MINGYUE YAOA
Keyword(s):  
Diabetes Mellitus ◽  
Body Weight ◽  
Blood Glucose ◽  
The Body ◽  
Antidiabetic Activity ◽  
Medicinal Uses ◽  
Diabetic Rabbit ◽  
Pharmaceutical Industries ◽  
Diabetic Rabbits ◽  
Plants Extract

The part of the plant to curing the diabetes mellitus and improves available data on plants with hypoglycemic effects. Antidiuretic potential of eucalyptus extracts has been evaluated in alloxan-induced diabetic rabbit. Here we used, plants extract about 256 kg were mix with 1000 ml methanol and keep in Flask. After filtering the extracts were exposed to rotary evaporator machine that methanol separated from plant extract. In the Nine rabbit were divided into three different groups. The body weight was recorded on every 6 days interval. The data obtained revealed that eucalyptus extracts reduced the glucose level effectively. Such treatment with extracts decreased the SGPT level. Therefore, it is concluded that the eucalyptus possesses significant antidiabetic activity. And finally, we recommend this plant for medicinal uses and for pharmaceutical industries to isolate pure drugs.

scholarly journals Evaluation of antihyperglycemic activities of Bangladeshi medicinal plant Cinnamomum tamala Leaf extracts in alloxan treated Albino Rats

2017 ◽  
Vol 7 (1) ◽  
pp. 11
Author(s):  
Abu Zaffar Shibly ◽  
Laila Arjumand Suborna ◽  
Juthi Adhikari ◽  
Md. Shariful Islam
Keyword(s):  
Body Weight ◽  
Blood Glucose ◽  
Medicinal Plant ◽  
Type Ii Diabetes Mellitus ◽  
Diabetic Control ◽  
Antidiabetic Activity ◽  
Albino Rats ◽  
Leaf Extracts ◽  
Glucose Levels ◽  
Blood Glucose Levels

Background: Diabetic mellitus is a multifactorial disorder associated with its devastating consequences has assumed epidemic proportion in Bangladesh.Methods: The study evaluates the anti-hyperglycemic activity of the aqueous extracts of C. tamala (CTLEt) leaves in blood glucose of albino rats. Type II diabetes mellitus was induced by injecting alloxan at the concentration of 100mg/kg body weight in male albino rats. The diabetic rats were administered orally with aqueous CTLEt at the amount of 1.0ml, 1.5ml and 2.0ml with lab diet and glibenclamide (5mg/kg of body weight). Then blood glucose levels were estimated in all groups after 2 hours, 4 hours, 6 hours, 12 hours and 18 hours of the treatment with CTLEt and a known antidiabetic drug glibenclamide.Results: A comparison was made between the action of CTLEt and glibenclamide. Blood glucose levels of the CTLEt on 18th hours of the study were 8.6 to 5.1mmol/L (1ml CTLEt with lab diet), 10.4 to 4.9mmol/L (1.5ml CTLEt with lab diet), 14.7 to 4.3mmol/L (2.0ml CTLEt with lab diet) in comparison of diabetic control (9.5 to 8.5, 8.7 to 7.8, 7.7 to 7.1mmol/L) and glibenclamide (13.9 to 6.5, 16.3 to 6.1, 9.5 to 5.1mmol/L). Among the sample level, the 2.0ml CTLEt showed a higher efficiency of hypoglycemic effect on alloxan induced diabetic rats.Conclusions: Till date, there is no specific experimental work in Bangladesh about the evolution of antidiabetic activity of C. tamala plant in animal model. Further studies should be undertaken to find out the molecular mechanism of the leaf powder of C. tamala medicinal plant.

scholarly journals Antidiabetic Activity of Sungkai (Peronema canescens Jack) Leaves Ethanol Extract on the Male Mice Induced Alloxan Monohydrate

2021 ◽  
Vol 6 (2) ◽  
pp. 64
Author(s):  
Madyawati Latief ◽  
Putri M. Sari ◽  
Liddini T. Fatwa ◽  
Indra L. Tarigan ◽  
H. P. Vasantha Rupasinghe
Keyword(s):  
Body Weight ◽  
Blood Glucose ◽  
Urine Volume ◽  
Ethanol Extract ◽  
Blood Glucose Measurement ◽  
Antidiabetic Activity ◽  
Glucose Measurement ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
Reduce Blood Glucose

  Sungkai (Peronema canescens Jack) is an alternative natural ingredient that can be used as an antidiabetic containing several secondary metabolites which are thought to reduce blood glucose levels by inhibiting the action of the α-glucokinase enzyme and increasing the activity of antioxidant enzymes and being able to regenerate β-cells, damaged pancreas so that insulin deficiency can be overcome. The aim of this study was to determine the effect of P. canescens leaves ethanol extract on antidiabetic activity and to determine the effect of various doses of sungkai leaf ethanol extract as antidiabetic. The scientific research method used in this study was a completely randomized design (CRD) and data analysis used in this study is the one way ANOVA, following by the Duncan test. Blood glucose measurement in treated mice was carried out 3 times. Mice were devided into five groups, three mice in each group, and each group was administered different dosages of sungkai extract (T1 = extract 175 mg/kg body weight; T2 = extract 350 mg/kg body weight; T3 = extract 700 mg/ kg body weight). First, the mice were acclimatized for six days and the injection of treatment was started at day 7. Blood glucose levels, urine volume, body weight and daily drinking of mice was carried out before induction (day 0), after induction (day 8) and after the end of treatment for 10 days (day 18), was carried out using the Point of Care Test (POCT) method using the Easy Touch GCU. The results showed that the ethanol extract of sungkai leaves had the potential to reduce blood glucose levels in mice. The ethanol extract of sungkai leaves at a dose of 350 mg/kg body weight has the most optimal potential to reduce blood glucose levels, urine volume, daily drinking, and body weight. 

scholarly journals Effect of Ethanolic Extract of Fruits of Eriobotrya japonica on Lipid Profile and Body weight in Streptozotocin Induced Diabetic Rats

2019 ◽  
Vol 11 (1) ◽  
Author(s):  
Sabeeha Shafi ◽  
Nahida Tabassum
Keyword(s):  
Body Weight ◽  
Blood Glucose ◽  
Lipid Profile ◽  
Ethanolic Extract ◽  
Diabetic Rats ◽  
The Body ◽  
Eriobotrya Japonica ◽  
Control Group ◽  
Glucose Levels ◽  
Blood Glucose Levels

Eriobotrya japonica locally called as loquat in Kashmir has been studied in various parts of the world but little work has been reported on Kashmiri loquat. The chemical nature of fruits and vegetables offers a great diversity of biological properties and plays an important role in the field of pharmacology. There is a quest for newer drugs with few adverse effects and this poses a challenge for the development of new drugs. The study was undertaken to study the activities of ethanolic extract of Eriobotrya japonica fruits in streptozotocin induced diabetic rats. The phytochemical screening of the plant was also done. The animals were divided into five groups. Normal Control group received only the vehicle. Toxic group included those animals in which diabetes was induced by streptozotocin. The 3rd group was those animals which received streptozotocin and standard antidiabetic drug-glibenclamide. 4th group included those diabetic animals which received 50 mg/kg b.w dose of fruits of Eriobotrya japonica. 5th group animals included those diabetic animals which received 100 mg/kg b.w of the plant extract. The biochemical parameters that were evaluated were blood glucose levels and lipid profile tests. The body weight was also checked. Histopathology of pancreas was also done. The results showed significant decrease in blood glucose levels, lipid profile tests in animals treated with different doses of the plant extracts. Histopathology of pancreas also showed positive results.

scholarly journals Hiperglikemia Tidak Menyebabkan Perubahan Bobot Badan dan Jumlah Neuron Mienterik Nitrergik Usus Halus Tikus Wistar (Rattus norvegicus)

2019 ◽  
Vol 36 (2) ◽  
pp. 258
Author(s):  
Amelia Hana ◽  
Pudji Astuti ◽  
Yuda Heru Fibrianto ◽  
Sarmin Sarmin ◽  
Claude Mona Airin ◽  
...  
Keyword(s):  
Body Weight ◽  
Blood Glucose ◽  
Rattus Norvegicus ◽  
The Body ◽  
Enteric Neurons ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
Group I ◽  
Wistar Strain ◽  
Small Intestines

Digestive dysfunction is a common symptom of diabetes. Diabetes can lead to the loss of enteric neurons as well as nerve dysfunction and gastrointestinal complications. There has been no complete report of mienteric nitrergic of small intestines on hyperglycaemia condition, so the purpose of this study was to assess changes of nitrergic neural profile of small intestines on hyperglycemic Wistar strain rats (Rattus norvegicus). Twelve rats were adapted for 3 days, then they were randomly divided into 3 groups namely group I (K-1, as control), group II (K-2), and group III (K-3). Before treatment all rats were fasted for 12 hours, rats K-1 were induced with 1.0 ml of buffer citrate single peritoneal dose; rats K-2 were induced with 40 mg streptozotocin (STZ)/ kg body weight of single peritoneal dose; and rats K-3 were induced with 45 mg STZ /kg body weight of single peritoneal dose. On the 1st and 5th days after STZ induction, blood was collected through the coccigea vein to measure blood glucose levels and weigh the weight. On the day 5th the rats were uthanated with a high dose of ketamine, dissected and taken small intestines (duodenum, jejunum, and ileum) for staining with Nicotinamide Adenine Dinucleotide Phosphate-diaphorase (NADPH-d). Morphometric changes of nitrergic neurons of the small intestine segments were observed. The results showed that on the 5th day increased blood glucose levels of the K-2 and K-3 groups were significantly higher than K-1 (P <0.01). The weight of three groups did not show any significant improvement difference (P> 0.05). The number of neurons in the duodenum, jejunum and ileum of K-2 group increased higher than that of K-1 and K-3 groups. These results indicate that in hyperglycaemic states, the number of nitrergic neurons increases (K-2 group), and there is a significant decrease in the number of neurons (group K-3) compared with K-1 and K-2 groups (P <0.01). The results of this study concluded, that the STZ induction treatment causes hyperglycemia. Hyperglycemia does not affect to the body weight and the number of  nitrergic neurons of small intestines in Wistar strain rats (Rattus norvegicus).

scholarly journals Comparison of Caralluma tuberculata with Metformin for Anti-Diabetic Activity: An Animal Study

2019 ◽  
Vol 8 (1) ◽  
pp. 34-39
Author(s):  
Muhammad Saleh Faisal ◽  
Waqar Hayat ◽  
Asad Inayat ◽  
Muhammad Sohaib ◽  
Waheed Iqbal
Keyword(s):  
Body Weight ◽  
Blood Glucose ◽  
Carbon Tetrachloride ◽  
Crude Extract ◽  
Gum Tragacanth ◽  
Glucose Levels ◽  
Cooking Oil ◽  
Blood Glucose Levels ◽  
Group I ◽  
Diabetic Rabbits

Background: Caralluma tuberculata, is a wild herb, which grows in the hills of Balochistan and has been known to have medicinal and nutritional properties since antiquity. This experimental research was designed to study the hypoglycemic properties of Caralluma tuberculata and to compare them with Metformin.Material and Methods: This was a laboratory-based animal experimental study. It was conducted in the Pharmacology laboratory of Khyber Medical University, Peshawar from February 2016 to August 2016. Two types of extracts of Caralluma tuberculata [crude extract and carbon tetrachloride (CCl4) extract] were prepared and administered to normal and alloxan treated diabetic rabbits. To study anti-diabetic activity, eighty-four rabbits were divided into two main groups. Group I (Normal/Non-Diabetic Rabbits; n= 21) and Group II (Diabetic/Alloxanized Rabbits; n=63). Each group was further divided into sub-groups (7 rabbits in each). Effect of Caralluma tuberculata, Metformin and 2% gum tragacanth on blood glucose levels were checked at 0, 2, 4, 6, 8, 12 and 24 hours of drug administration. The extracts were given in capsule form and in cooking oil. Data analysis was done using SPSS version 16. For calculation and comparison of the hypoglycemic effects at various doses and different time intervals, analysis of variance (ANOVA) and Tukey’s post hoc test were applied.Results: The crude extract, 200mg/kg body weight of Caralluma tuberculata showed significant decrease (p<0.001) in mean blood glucose levels from 2-hour till12 hours. Whereas, highly significant reduction of blood glucose was seen from 2 hours after treatment till 24 hours, when carbon tetrachloride (CCl4) fraction of Caralluma (100mg/kg body weight) in capsule form was administered. Metformin 500mg/kg body weight was given to compare its effects with plant crude extract and it was found that metformin appeared to be less effective in comparison with Caralluma tuberculata.Conclusion: Caralluma tuberculata lowered the blood glucose level and turned out to be more significant in developing hypoglycemia when taken with cooking oil. More work is essential to provide stronger evidence for the use of this natural agent in the management of Diabetes Mellites.

scholarly journals ANTI-HYPER LIPIDEMIC ACTIVITY OF METHANOLIC EXTRACT OF SMILAX WIGHTII A.DC. IN STREPTOZOTOCIN INDUCED MALE WISTAR ALBINO RATS

2014 ◽  
Vol 1 (2) ◽  
pp. 126-130
Author(s):  
Uma Maheswari P ◽  
Arumugasamy K ◽  
Shalimol A ◽  
Asha devi V ◽  
Nantha kumar R
Keyword(s):  
Body Weight ◽  
Blood Glucose ◽  
Plant Extract ◽  
Methanolic Extract ◽  
Fasting Blood Glucose ◽  
The Body ◽  
Lipid Lowering ◽  
Control Group ◽  
Glucose Levels ◽  
Blood Glucose Levels

Smilax wightii, an endemic medicinal plant is found in the shola forests at high altitudes in Nilgiri Biosphere Reserve, the Western Ghats, Southern India . The present study, was undertaken to find out the effect of methanolic extract of this plant on the body weight, fasting blood glucose levels and lipid profiles inall the streptozotocin (STZ) induced rats. The extract exerted a significant (P<0.05) effect in the body weight of the experimental animals when compared to the control group. Treatment with the extract and glibenclamide resulted in a significant (P<0.01) reduction in the fasting blood glucose levels in diabetic ratswhen compared to the normal. The lipid profile such as TC, TG, LDL, and VLDL contents in the serum registered a significant (P<0.01) hike and a decline in the HDL contents in diabetic control group, which were retrieved to near normalcy in the plant extract treated groups. The effect produced by this plant extract wascomparable with that of glibenclamide. The decreased fasting blood glucose levels and lipid lowering properties clearly showed the anti-hyperlipidemic effect of S.wightii.

scholarly journals Ameliorative effect of crude and solvent partitioned fractions of Gymnema sylvestre in alloxan-induced hyperglycemic and dyslipidemic Rattus norvegicus

2021 ◽  
Vol 1 (2) ◽  
pp. 11-23
Author(s):  
Rofiyat Omolara Raji ◽  
Hadiza Lami Muhammad ◽  
Hussaini Anthony Makun ◽  
Musa Bola Busari ◽  
Sophia Shekwoyan Maikai ◽  
...  
Keyword(s):  
Body Weight ◽  
Blood Glucose ◽  
Serum Lipid ◽  
Diabetic Rats ◽  
The Body ◽  
Lipoprotein Cholesterol ◽  
Gymnema Sylvestre ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
Ameliorative Effect

The present study aims to evaluate the ameliorative effect of crude and solvent partitioned fractions of Gymnema sylvestre in alloxan-induced hyperglycemic and dyslipidemic rats. The extract and fractions of Gymnema sylvestre were administered to alloxan (120 mg/kg) induced diabetic rats at doses of 100, 300, and 600 mg/kg. Metformin (100 mg/kg) was used as the reference drug. All treatments were administered daily for 14 days through the oral route. Effects of the treatments on blood glucose levels, body weight, and serum lipid profiles were monitored. The results revealed that all the extracts and fractions were able to reduce the blood glucose levels and improved the body weight of diabetic rats, with more pronounced effects at the treatment of 300 mg/kg of crude methanol and ethyl acetate fraction. The blood glucose of diabetic untreated rats continued to increase up to the time of euthanization. There were significant reductions (p<0.05) in the levels of serum total cholesterol, triglyceride, and low-density lipoprotein cholesterol (LDL-C) of all the treated groups when compared to the diabetic untreated. However, a significant (p<0.05) decrease in the serum concentration of highdensity lipoprotein- cholesterol (HDL- C) was observed in the diabetic untreated rats when compared to the HDL-C levels in the treated rats. The ameliorative effect of the crude extract and the partitioned fractions on the diabetic induced alterations in serum lipid profile occurs in a significant (p<0.05) dosedependent manner. In conclusion, G. sylvestre extracts possess hypoglycaemic effects and attenuated diabetic induced dyslipidemia in rats

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