scholarly journals Antidiabetic Activity of Sungkai (Peronema canescens Jack) Leaves Ethanol Extract on the Male Mice Induced Alloxan Monohydrate

2021 ◽  
Vol 6 (2) ◽  
pp. 64
Author(s):  
Madyawati Latief ◽  
Putri M. Sari ◽  
Liddini T. Fatwa ◽  
Indra L. Tarigan ◽  
H. P. Vasantha Rupasinghe
Keyword(s):  
Body Weight ◽  
Blood Glucose ◽  
Urine Volume ◽  
Ethanol Extract ◽  
Blood Glucose Measurement ◽  
Antidiabetic Activity ◽  
Glucose Measurement ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
Reduce Blood Glucose

  Sungkai (Peronema canescens Jack) is an alternative natural ingredient that can be used as an antidiabetic containing several secondary metabolites which are thought to reduce blood glucose levels by inhibiting the action of the α-glucokinase enzyme and increasing the activity of antioxidant enzymes and being able to regenerate β-cells, damaged pancreas so that insulin deficiency can be overcome. The aim of this study was to determine the effect of P. canescens leaves ethanol extract on antidiabetic activity and to determine the effect of various doses of sungkai leaf ethanol extract as antidiabetic. The scientific research method used in this study was a completely randomized design (CRD) and data analysis used in this study is the one way ANOVA, following by the Duncan test. Blood glucose measurement in treated mice was carried out 3 times. Mice were devided into five groups, three mice in each group, and each group was administered different dosages of sungkai extract (T1 = extract 175 mg/kg body weight; T2 = extract 350 mg/kg body weight; T3 = extract 700 mg/ kg body weight). First, the mice were acclimatized for six days and the injection of treatment was started at day 7. Blood glucose levels, urine volume, body weight and daily drinking of mice was carried out before induction (day 0), after induction (day 8) and after the end of treatment for 10 days (day 18), was carried out using the Point of Care Test (POCT) method using the Easy Touch GCU. The results showed that the ethanol extract of sungkai leaves had the potential to reduce blood glucose levels in mice. The ethanol extract of sungkai leaves at a dose of 350 mg/kg body weight has the most optimal potential to reduce blood glucose levels, urine volume, daily drinking, and body weight. 

scholarly journals Phytochemical Screening and Antidiabetic Activity Test of Extracts and Fractions of Lactuca Indica (L.) In Streptozotocin-Induced Diabetic Mice

1970 ◽  
Vol 8 (3) ◽  
pp. 62-65
Author(s):  
Tamba Rosanto ◽  
Nainggolan Marline ◽  
Rosidah Noersal
Keyword(s):  
Blood Glucose ◽  
Ethanol Extract ◽  
Negative Control ◽  
Antidiabetic Activity ◽  
Antidiabetic Effect ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
Group I ◽  
Reduce Blood Glucose ◽  
Lactuca Indica

Objectives: The purpose of this study was to determine the antidiabetic effect of ethanol extract and Lactuca indica leaf fraction to determine the effective dose of mice induced with streptozotocin compared with metformin. Design: The design of this study was experimental in which the extraction and fraction of Lactuca indica leaves were tested for the value of decreasing blood glucose levels of mice after induction of streptozotocin. Antidiabetic activity tests were divided into 12 groups. Group I (baseline) group II (negative control) were given CMC 0.5%, Group III (positive control) were given metformin 65 mg/kg BW, while Groups IV to XII were given Lactuca indica leaf extract and fractions at their respective doses -each 100, 150 and 200 mg/kg BW Interventions: The variable that was intervened in this study was the concentration of extract used Main outcome measure: The main measurement results in this study were to determine extracts and fractions that we're able to reduce blood glucose levels in mice. Results: The antidiabetic effect of Lactuca indica leaves shows that EELL, EAFLL and NHFLL have antidiabetic effects, this is supported by the chemical content contained in the extracts, namely flavonoids, tannins, saponins, glycosides and triterpenoids/steroids. The most effective activity to reduce blood glucose levels in streptozotocin-induced mice is EAFLL 100 mg/kg BW Conclusion: ethyl acetate fraction of Lactuca indica leaves has an effective antidiabetic activity at a dose of 200 mg/kg BW has given antidiabetic activity in mice induced by STZ, on the 9th day BGL mice have dropped below 120 mg/dl.  

scholarly journals Antidiabetic activities of 96% ethanol extract of Abelmoschus esculentus on Balb-C mice strain

2020 ◽  
Vol 4 (2) ◽  
Author(s):  
Agnis Pondineka Ria Aditama
Keyword(s):  
Antioxidant Activity ◽  
Blood Glucose ◽  
Ethanol Extract ◽  
Negative Control ◽  
Abelmoschus Esculentus ◽  
Antidiabetic Activity ◽  
Male Mice ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
Reduce Blood Glucose

Diabetes mellitus (DM) is a progressive metabolic disease that continues to increase every year. Treatment therapy using oral hypoglycemic drugs to treat DM has side effects, and alternative approaches must be taken. Abelmoschus esculentus has been known to be used as an alternative to DM because it has antioxidant activity due to its high flavonoid content. The purpose of this study was to determine the antidiabetic activity of 96% ethanol extract of Okra leaves against male mice of balb-c strain. Male mice were acclimatized for seven days and divided into five test groups and then fasted for 18 hours. Then the mice were induced with 150 mg / 20 g BW alloxan and measured on the 3rd day. Ethanol extract 96% okra leaves were then given in a dose of 5.6 mg / 20g BW; 11.2 mg / 20g BW; 22.4 mg / 20g BW, CMC Na 0.5% as negative control and glibenclamide 0.026 mg / 20g BBs as positive control for 14 days. The results of therapy with 96% ethanol extract of okra leaves can reduce blood glucose levels in mice so that the okra leaves contain flavonoid compounds that have antioxidant activity and can reduce blood glucose levels.

scholarly journals AKTIVITAS ANTIDIABETES EKSTRAK ETANOL DAUN BELIMBING BESI (Averhhoa carrambola) PADA MENCIT (Mus musculus)

2021 ◽  
Vol 9 (2) ◽  
pp. 74
Author(s):  
NI PUTU SRI PUSPITA WIDI YANTHI ◽  
Ni Putu Adriani Astiti ◽  
Ni Wayan Sudatri
Keyword(s):  
Blood Glucose ◽  
Ethanol Extract ◽  
Negative Control ◽  
Antidiabetic Activity ◽  
Antioxidant Compounds ◽  
Experimental Animals ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
Star Fruit ◽  
Reduce Blood Glucose

Many antioxidant compounds, flavonoids, and saponins are contained in starfruit leaves (Averrhoa carambola). The purpose of this study was to determine the antidiabetic activity of star fruit extract in mice. This study used a completely randomized design (CRD) with 6 treatments, namely P1 (aquadest without alloxan) as a negative control, P2 (aquadest with alloxan) as a positive control, P3 (metformin 500 mg / kgBB), P4 (alloxan + ethanol extract of star fruit leaves. 100 mg / kgBB), P5 (alloxan + ethanol extract of star fruit leaves 150 mg / kgBB), P6 (alloxan + ethanol extract of star fruit leaves 200 mg / kgBB). The starfruit leaf extract was made using the maceration method. In order for experimental animals to become diabetic, alloxan is used at a dose of 120 mg / kgBW which is injected intramuscularly into the thigh muscles. After the experimental animals were diabetic, We were given ethanol extract of starfruit leaves according to the experimental design, starting from day 7th to day 21st. Blood glucose levels are measured with a glucometer every week. The results showed that the best decrease in blood glucose levels occurred in the first week and the second week. In the first week, the metformin treatment was able to reduce blood glucose by 88.4%, while the ethanol extract treatment of star fruit leaves at a dose of 200 mg / kg BW was able to reduce blood glucose levels by 60%. The ethanol extract of star fruit leaves has antidiabetic activity. Keywords: mice, diabetes mellitus, blood glucose, starfruit leaves.  

Gynura divaricata Effect on Blood Glucose Levels in Alloxan-Induced Diabetes Mice

2019 ◽  
Vol 13 (01) ◽  
pp. 27-33
Author(s):  
Evi Sovia ◽  
Dian Anggraeny ◽  
Ris Kristiana ◽  
Firhan Hamdi Maulida ◽  
Mutia Susparini
Keyword(s):  
Blood Glucose ◽  
Histological Examination ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Antidiabetic Activity ◽  
Phytochemical Analysis ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
Diabetes Mice ◽  
Before And After

Gynura divaricata (GD) also known as Dewa leaf is one of the Indonesian medicinal plants which are also native to India, Thailand and China. GD is widely used as antidiabetic, antihypertensive and other diseases including several tumors. However, research on the effects of GD as antidiabetic is still not widely practiced, especially in Indonesia. The aim of this research is to investigate the effect of GD ethanolic extract on blood glucose levels of alloxan-induced diabetes mice. This research is an experimental research with pre- and post-test design using 24 male DDY strain mice. Animals experiment is divided into four groups, that are one normal group and three alloxan-induced diabetes mice group that give 1% CMC (control), 400[Formula: see text]mg/kg GD ethanolic extract and 0.65[Formula: see text]mg/kg glibenclamide, respectively. Treatment was given for 14 days. Blood glucose levels were measured before and after inducing alloxan, and after 14 days treatment. At the end of study, all of animals experiment were sacrificed for histological examination. Phytochemical analysis revealed that polyphenols, flavonoids, steroids, triterpenoids, terpenoids and and quinones were present in ethanol extract of GD. The results of this study showed significant ([Formula: see text]) decrease of blood glucose levels after GD ethanolic extract and glibenclamide treatment. The results of histological examination showed improvement in pancreas damage and significant increase in the number of beta cells in GD and glibenclamide groups. The results indicate that dewa leaf ethanolic extract has antidiabetic activity and the effectiveness is the same as glibenclamide. GD also could repair damage of pancreas caused by alloxan induction.

scholarly journals Skrining Fitokimia dan Potensi Antidiabetes Ekstrak Etanol Herba Ciplukan (Physalis Angulata L) pada Tikus Putih (Rattus Novergicus) yang Diinduksi Aloksan

2019 ◽  
Vol 4 (3) ◽  
pp. 98-107
Author(s):  
Hendra Pratama Maliangkay ◽  
Rolef Rumondor ◽  
Mynia Kantohe
Keyword(s):  
Blood Glucose ◽  
Rattus Norvegicus ◽  
Ethanol Extract ◽  
Negative Control ◽  
Glucose Measurement ◽  
Percentage Decrease ◽  
Glucose Levels ◽  
White Rats ◽  
Blood Glucose Levels ◽  
Physalis Angulata

The purpose of this study to determine the anti-diabetic potential of ciplukan herbal ethanol extract (Physalis angulata L) in white rats (Rattus norvegicus) induced alloxan given intraperitoneally and comparing its effectiveness with glibenclamide. This study uses 5 groups namely giving aquadest as normal control, giving alloxan as a negative control, giving alloxan and glibenclamide as a positive control and the ethanol extract of ciplukan herbal with a dose of 150 mg/kg BB and 300 mg/kg BB. Blood glucose measurement performed on day 0 (three days after induction), day 7, and day 14. The results showed that ethanol extract of ciplukan herbal (Physalis angulata L) can lower blood glucose levekls. A dose of 150 mg/kg BB have percentage decrease in blood glucose levels on day 7 and day 14 respectively by 14.1%and 70.5%. dose of 300 mg/kg BB have a percentage decrease in blood glucose levels on day 7 and day 14 respectively by 70.5%.and 69.5%. a dose of 150 mg/kg BB is more effective as an anti-diabetic compared with a dose of 300 mg/kg BB. Ethanol extract of ciplukan herbal (Physalis angulata L) also has an activity to repair damaged pancreatic-β cells in rats (Rattus norvegicus) induced by alloxan.

scholarly journals UJI AKTIVITAS EKSTRAK ETANOL DAUN KERSEN (Muntingia calabura L.) SEBAGAI INHIBITOR ENZIM α-GLUKOSIDASE DENGAN MENGGUNAKAN ELISA READER

2020 ◽  
Vol 7 (2) ◽  
pp. 1-5
Author(s):  
Sukmawati Syarif ◽  
Nurnaningsih Nurnaningsih ◽  
Mamat Pratama
Keyword(s):  
Blood Glucose ◽  
Wistar Rats ◽  
Ethanol Extract ◽  
Antidiabetic Activity ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
Sample Extract ◽  
Male Wistar Rats ◽  
Muntingia Calabura

Kersen leaves (Muntingia calabura L) contain metabolites such as alkaloids, anthrax, polyphenols, tannins, saponins and are rich in flavonoid components such as flavones, flavonones, flavan and biflavan which have antidiabetic activity. According to a study conducted by Apriyanti (2016), the ethanol extract of kersen (Muntingia calabura L) at a dose of 250 mg / kg BB significantly reduced blood glucose levels in male wistar rats. In this study aimed at determining IC50 ethanol extract of Muntingia calabura L as an inhibitor of α-glucosidase enzymes using ELISA reader. The method was divided into 3 category is 1 (sample extract), 2 (blank) and 3 (akarbose). Each group added 25 µL α-glucosidase solution (0.25 units / mL then measured using ELISA reader 405 nm. The results showed that the ethanol extract of kersen (Muntingia calabura L) had activity as an inhibitor of α-glucosidase enzyme with IC50 34,197 µg / mL and can be categorized as active.

Antidiabetic Activity of Peperomia pellucida In Streptozotocin-Induced Diabetic Mice

2021 ◽  
Vol 7 (2) ◽  
pp. 120-129
Author(s):  
Sholihatil Hidayati
Keyword(s):  
Blood Glucose ◽  
Ethanol Extract ◽  
Hexane Fraction ◽  
Antidiabetic Activity ◽  
Diabetic Mice ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
Potential Development ◽  
Lower Blood ◽  
Development Objectives

Background: Diabetes mellitus is a heterogeneous group of diseases in the form of disorders in the body's metabolism clinically. Peperomia pellucida herbs have phytochemical containing which is antidiabetic potential development. Objectives: This study was conducted to compare the antidiabetic activity of ethanol extract and n-hexane fraction of Peperomia pellucida. Material and Methods: This research was conducted by make diabetic mice with 50 mg/kg.bw of streptozotocin induction, which was then treated with ethanol extract and n-hexane fraction of Peperomia pellucida with doses 250 mg/kgbw for 7 days.  Results: The results showed that the ethanol extract and n-hexane fraction of Peperomia pellucida reduced blood glucose levels in diabetic mice due to streptozotocin induction. The n-hexane fraction of Peperomia pellucida can lower blood glucose levels as much 244.00 ± 18.99 mg/dL better than the ethanol extract, which is 99.50 ± 28.17 mg/dL. Conclusions: Peperomia pellucida herb has the potential to be developed as an antidiabetic agent.

scholarly journals Evaluation of antihyperglycemic activities of Bangladeshi medicinal plant Cinnamomum tamala Leaf extracts in alloxan treated Albino Rats

2017 ◽  
Vol 7 (1) ◽  
pp. 11
Author(s):  
Abu Zaffar Shibly ◽  
Laila Arjumand Suborna ◽  
Juthi Adhikari ◽  
Md. Shariful Islam
Keyword(s):  
Body Weight ◽  
Blood Glucose ◽  
Medicinal Plant ◽  
Type Ii Diabetes Mellitus ◽  
Diabetic Control ◽  
Antidiabetic Activity ◽  
Albino Rats ◽  
Leaf Extracts ◽  
Glucose Levels ◽  
Blood Glucose Levels

Background: Diabetic mellitus is a multifactorial disorder associated with its devastating consequences has assumed epidemic proportion in Bangladesh.Methods: The study evaluates the anti-hyperglycemic activity of the aqueous extracts of C. tamala (CTLEt) leaves in blood glucose of albino rats. Type II diabetes mellitus was induced by injecting alloxan at the concentration of 100mg/kg body weight in male albino rats. The diabetic rats were administered orally with aqueous CTLEt at the amount of 1.0ml, 1.5ml and 2.0ml with lab diet and glibenclamide (5mg/kg of body weight). Then blood glucose levels were estimated in all groups after 2 hours, 4 hours, 6 hours, 12 hours and 18 hours of the treatment with CTLEt and a known antidiabetic drug glibenclamide.Results: A comparison was made between the action of CTLEt and glibenclamide. Blood glucose levels of the CTLEt on 18th hours of the study were 8.6 to 5.1mmol/L (1ml CTLEt with lab diet), 10.4 to 4.9mmol/L (1.5ml CTLEt with lab diet), 14.7 to 4.3mmol/L (2.0ml CTLEt with lab diet) in comparison of diabetic control (9.5 to 8.5, 8.7 to 7.8, 7.7 to 7.1mmol/L) and glibenclamide (13.9 to 6.5, 16.3 to 6.1, 9.5 to 5.1mmol/L). Among the sample level, the 2.0ml CTLEt showed a higher efficiency of hypoglycemic effect on alloxan induced diabetic rats.Conclusions: Till date, there is no specific experimental work in Bangladesh about the evolution of antidiabetic activity of C. tamala plant in animal model. Further studies should be undertaken to find out the molecular mechanism of the leaf powder of C. tamala medicinal plant.

scholarly journals Uji Efek Antidiabetik Ekstrak Daun Trembesi (Albizia saman (Jacq.) Merr) Terhadap Mencit (Mus musculus L)

WARTA FARMASI ◽  
2019 ◽  
Vol 8 (2) ◽  
pp. 43-52
Author(s):  
Muhammad Azdar Setiawan ◽  
Muhammad Syaiful Saehu ◽  
Kartini Kartini
Keyword(s):  
Body Weight ◽  
Blood Glucose ◽  
Mus Musculus ◽  
Leaf Extract ◽  
Positive Control ◽  
Negative Control ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
Reduce Blood Glucose ◽  
Albizia Saman

ABSTRAK Daun trembesi (Albizia saman (Jacq.) Merr) adalah salah satu tanaman berkhasiat menurunkan kadar glukosa darah dimana kandungan kimianya berpotensi menurunkan kadar glukosa darah antara lain flavonoid yang bekerja dengan cara menstimulasi sel- sel beta dari pulau langerhans, sehingga sekresi insulin ditingkatkan.Penelitian ini bertujuan untuk mengetahui efek antidiabetes ekstrak daun trembesi (Albizia saman (Jacq.) Merr) pada mencit (Mus musculus). Metode Penelitian ini merupakan penelitian Eksperimen dimana sebanyak 20 ekor mencit dibuat diabetes dengan menggunakan Streptozotosin 150 mg/kgBB secara Intraperitonial. Kemudian dibagi menjadi 5 kelompok perlakuan, yaitu: Ekstrak dosis 25 mg/kgBB, 50 mg/kgBB, 100  mg/kgBB, sebagai kontrol positif Glibenclamid 5 mg dan kontrol negatif suspensi Na.CMC 0,5%. Data yang diperoleh dianalisis dengan uji ANOVA. Hasil analisa statistik menunjukan pada konsentrasi ekstrak 100 mg/kgBB memberikan efek yang optimum dengan perlakuan kontrol positif Glibenklamid.  Kata Kunci  : Ekstrak Daun Trembesi, Antidiabetik.  ABSTRACT Trembesi leaf (Albizia saman (Jacq.) Merr) is one of the efficacious plants to reduce blood glucose levels where the chemical content has the potential to reduce blood glucose levels, among others, flavonoids that work by stimulating beta cells of the island langerhans, so that insulin secretion is increased. It aims to determine the antidiabetic effect of trembesi leaf extract (Albizia saman (Jacq.) Merr) in mice (Mus musculus). This research method is an experimental study in which as many as 20 mice were made diabetic using Streptozotosin 150 mg / kgBW intraperitonially. Then divided into 5 treatment groups, namely: Extract dose 25 mg / kg body weight, 50 mg / kg body weight, 100 mg / kg body weight, as positive control Glibenclamid 5 mg and negative control suspension Na.CMC 0.5%. The data obtained were analyzed by ANOVA test. The results of statistical analysis showed that the extract concentration of 100 mg / kgBB gave the optimum effect with the positive control of Glibenclamide. Keywords: Trembesi Leaf Extract, Antidiabetic.

scholarly journals NANOEMULSION OF OKRA FRUIT EXTRACT AS ANTIDIABETIC TREATMENT

2020 ◽  
pp. 138-142
Author(s):  
RATNA DJAMIL ◽  
SARAH ZAIDAN ◽  
DENI RAHMAT ◽  
DIAH KARTIKA PRATAMI ◽  
FELIX HAKIM
Keyword(s):  
Blood Glucose ◽  
Ethanol Extract ◽  
Positive Control ◽  
Negative Control ◽  
Antidiabetic Activity ◽  
Control Group ◽  
Glucose Levels ◽  
Antidiabetic Treatment ◽  
Blood Glucose Levels ◽  
Average Blood Glucose

Objective: Okra (Abelmoschus esculentus (L.) Moench) has potential antidiabetic activity. This study created a nanoemulsion of okra extract (NOE) and examined its activity on alloxan-induced diabetes mellitus in mice. Methods: Okra was macerated with 70% ethanol and dried in a rotary evaporator into the crude extract. The extract was encapsulated in a solution of glyceryl caprylate, propylene glycol, and glycerine to form a nanoemulsion. To determine the antihyperglycaemic effect of okra extract, 35 male mice (Mus musculus L.) were divided into seven groups: a non-diabetic normal control group and six diabetic mice groups (untreated negative control, glibenclamide-treated positive control, and four treatments with okra ethanol extract (OEE) at 200 and 400 mg/kg BW and NOE at 200 and 400 mg/kg BW). Results: The group treated with NOE at 400 mg/kg BW (NOE400) had the lowest average blood glucose level of 93.4 mg/dL among hyperglycaemic mice. The decrease in blood glucose levels in NOE400 (52.05%) was significantly different from those in the positive control (42.63%) and OEE treatments (39.32%). The nanoemulsion used in this study fulfills quality requirements, with a mean particle size of 134.7 nm, a polydispersity index of 0.512, and a zeta value of −26.72 mV. Conclusion: NOE reduced blood glucose levels in alloxan-induced hyperglycaemic mice better than OEE. Nanoemulsion can improve the antidiabetic activity of okra extract by increasing penetration of active compounds into interstitial space so that their delivery and bioavailability are higher.

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