Association of Hemoglobin Concentration and Cord Blood Cell Characteristics in Healthy Newborn Infants.

Blood ◽  
2009 ◽  
Vol 114 (22) ◽  
pp. 5092-5092
Author(s):  
Mikko Eskola ◽  
Sari Juutistenaho ◽  
Kari Aranko ◽  
Susanna Sainio ◽  
Riitta Kekomäki

Abstract Abstract 5092 BACKGROUND AND OBJECTIVE Cord blood (CB), used for hematopoietic stem cell transplantation, is readily available from CB banks, and the banking processes are widely standardized. The data collected in CB banks have been utilized to characterize the contents of CB units and, to some extent, the physiological phenomena of healthy newborn infants. The objective of this study was to evaluate the association of the hemoglobin concentration (Hb), whose measurement is independent of the cell counts, with CB cell characteristics (red blood cells, RBC, hematocrit, Hct, mean platelet volume, MPV, platelets, PLT and white blood cells, WBC). The internal associations of cell counts were also evaluated. MATERIALS AND METHODS The study comprised 167 consecutive ex utero CB collections accepted for processing. All the infants (52% male) were healthy and delivered at term. Of the deliveries 104 (62%) were by Cesarean section. Perinatal data were obtained from the maternity hospital records. The relative birth weight was expressed as a birth weight z-score to normalize the birth weight for gestational age and gender. The median birth weight was 3784 g (range 2490 – 4975) and the median relative birth weight 0.49 SD (-2.41 – 3.37). The mode of delivery did not significantly influence these values. Umbilical artery (UA) pH samples were drawn after delivery as part of routine hospital care. A hematology analyzer utilizing the electronic resistance detection method coupled with volumetric (cell analysis) and colorimetric (Hb) principles was used for cell counting (Sysmex K1000, Sysmex Corp., Kobe, Japan). All the concentrations were standardized to exclude the effect of the varying blood-to-anticoagulant ratio. (Aroviita et al. Acta Paediatr 2004;93:1323-1329) CD34+ cell analysis was performed with a flow cytometer based on ISHAGE guidelines. Two-sided p values <0.05 were considered statistically significant. The CB banking program was approved by the ethical committees of the institutes. RESULTS The hematological values and their distributions were within the published ranges. (Nathan and Oski's Hematology of infancy and childhood, 7th ed. 2009) Hb (median 174 g/L, range 130 – 234) correlated with the RBC concentration (4.70 ×1012/L, 3.46 – 6.62; r = 0.96, p < 0.0001), as expected. Interestingly, MPV (8.7 fL, 7.5 – 11.5) correlated with Hb (r = 0.22, p = 0.0043), RBC concentration (r = 0.26, p = 0.0008), and Hct (53.6%, 40.1 – 73.1; r = 0.26, p = 0.0008). The PLT concentration (270 × 109/L, 161 – 607) and plateletcrit (defined as PLT concentration x MPV; 0.24%, 0.15 – 0.48) correlated negatively with Hb (r = -0.31, p < 0.0001 and r = -0.26, p = 0.0008, respectively). Hb also correlated with the WBC concentration (15.1 ×109/L, 5.54 – 39.7; r = 0.23, p = 0.0024). No correlation was observed between UA pH (7.28, 7.04 – 7.4) and the CB cell counts, except for the WBC concentration (r = -0.36, p < 0.0001) and CD34+ cell concentration (43.9 ×106/L, 7.14 - 253; r = -0.37, p < 0.0001). The CB PLT concentration and UA pH differed significantly between vaginal delivery and Cesarean section (p = 0.0053 and p = 0.0002, respectively). CONCLUSIONS Valuable information about neonatal hematology can be obtained through careful analysis of perinatal and CB banking data. Hb and the cell characteristics of CB reveal strictly controlled internal associations which seem to reflect the general activity of hematopoiesis. Disclosures No relevant conflicts of interest to declare.

1976 ◽  
Vol 35 (03) ◽  
pp. 712-716 ◽  
Author(s):  
D. Del Principe ◽  
G Mancuso ◽  
A Menichelli ◽  
G Maretto ◽  
G Sabetta

SummaryThe authors compared the oxygen consumption in platelets from the umbilical cord blood of 36 healthy newborn infants with that of 27 adult subjects, before and after thrombin addition (1.67 U/ml). Oxygen consumption at rest was 6 mμmol/109/min in adult control platelets and 5.26 in newborn infants. The burst in oxygen consumption after thrombin addition was 26.30 mμmol/109/min in adults and 24.90 in infants. Dinitrophenol did not inhibit the burst of O2 consumption in platelets in 8 out of 10 newborn infants, while the same concentration caused a decrease in 9 out of 10 adult subjects. Deoxyglucose inhibited the burst in O2 consumption in newborn infant and adult platelets by about 50%. KCN at the concentration of 10−4 M completely inhibited basal oxygen consumption but did not completely inhibit the burst after thrombin. At the concentration of 10−3 M, it inhibited both basal O2 consumption and the burst in infants and adult subjects.


PEDIATRICS ◽  
1970 ◽  
Vol 45 (1) ◽  
pp. 155-155
Author(s):  
Judith M. Chessells ◽  
W. R. Pitney

Drs. Stiehm and Clatanoff (Pediatrics, 43: 770, 1969) report a high incidence of split products of fibrin (SPF) in the cord blood of healthy newborn infants. This is contrary to our own experience which had indicated the presence of SPF in only 5% of healthy newborns. We use a hemagglutination inhibition technique which is at least as sensitive as the tube precipitin assay employed by Drs. Stiehm and Clatanoff. The two methods differ in one important aspect, however; we have tested cord blood drawn into a mixture of sodium citrate and epsilon amino-caproic acid to inhibit in vitro fibrinolysis.


2009 ◽  
Vol 61 (4) ◽  
pp. 791-796 ◽  
Author(s):  
M.L.B. Cápua ◽  
A.E. Santana ◽  
A.P.M. Nakage ◽  
A.V. Godoy ◽  
A. Kataoka

The hematological parameters red blood cells (RBC) and total white blood cells (WBC) counts, hematocrit, hemoglobin concentration, and RBC indexes (median corpuscular volume and median corpuscular hemoglobin concentration) were determined and T CD5+ lymphocytes and CD4+ and CD8+ subpopulations of the umbilical cord blood (UCB) of dogs were quantified by the cytofluorimetric technique. Nine adult Beagles, from two do five-year old, were used as control. The umbilical cord blood (UCB) was collected from 20 neonate dogs. The method for the UCB collection was adequate to obtain sufficient quantity of blood for the accomplishment of the hematological analyses and lymphocyte quantification. Cytoscopic preparations of the UCB suggested high erythropoietic activity. There was no difference for the global leukocyte and lymphocyte counts between the groups. UCB T lymphocyte counts were lower than those obtained for adult dogs. The proportion of CD4:CD8 showed a great dominance of T CD4+ cells over T CD8+ lymphocytes in UCB.


Blood ◽  
1974 ◽  
Vol 43 (3) ◽  
pp. 357-361 ◽  
Author(s):  
Søren Knudtzon

Abstract Human umbilical cord blood cells from 26 newborn infants and peripheral blood cells from 18 adults were cultured in vitro by using the agar-gel method of human hemopoietic cell culture. An increased concentration of colony-forming cells was seen in the cord blood cultures. Between 17 and 385 colonies, with a mean of 122, were formed in these cultures per 2 x 105 nucleated cells plated. The peripheral blood cell cultures from adults gave rise to 0-11 colonies, with a mean of 3, per 2 x 105 nucleated cells plated. The average number of cells per colony was 1000-1500 cells after 14 days of culture, predominantly granulocytic.


PEDIATRICS ◽  
1958 ◽  
Vol 22 (4) ◽  
pp. 715-726
Author(s):  
Louise Lang Phillips ◽  
Valija Skrodelis

Studies of the fibrinolytic enzyme system in the plasma of mothers and the newborn infants are reported and the results compared. All mothers had elevated levels of fibrinogen in the plasma at the time of delivery. The levels of fibrinogen in the newborn infants fell into a low normal range. Premature infants tended to have slightly lower levels of fibrinogen than term infants, with certain exceptions which are discussed. A direct correlation of values for fibrinogen with the birth weight could not be established. No correlation was observed between fibrinogen levels in mothers and infants, indicating that significant placental transfer of fibrinogen does not occur under normal conditions and that fibrinogen is manufactured by the fetus itself. All mothers had higher levels of free and total profibrinolysin in the plasma than did the infants. The differences were found to be highly significant. The levels of free profibrinolysin in premature infants did not vary as much from those of the term babies, as was the case with total profibrinolysin. The levels of total profibrinolysin show a definite trend upward with increasing birth weight. Inhibitors of the fibrinolytic enzyme system were also significantly higher in mothers than in infants. Premature infants had significantly lower levels than term infants although considerable overlapping in the range was noted. Evidence was obtained that small amounts of an active proteolytic enzyme are present in the euglobulin fraction of the mother at delivery even after the course of a normal labor. No hemorrhagic manifestations were observed, presumably because of the high levels of inhibitor in maternal blood. In contrast, lysis of clots was observed more frequently in samples of cord blood, possibly due to lower levels of inhibitor. Placental transfer of various decomposition products of protein apparently exists as indicated by measurements of trichloracetic acid-soluble products in samples of plasma. A possible relation between the low proteolytic activity of the plasma of premature infants and the high incidence of fatality from hyaline membrane disease in these infants is proposed and discussed.


PEDIATRICS ◽  
1969 ◽  
Vol 43 (5) ◽  
pp. 770-780
Author(s):  
E. Richard Stiehm ◽  
Dallas V. Clatanoff

Levels of split products of fibrin (SPF), as measured by an immunologic tube precipitin method, were studied in cord and maternal sera obtained shortly after delivery. One hundred thirty-seven of 208 (65%) of vaginally delivered, term newborns, 28 of 48 (58%) of cesarean section delivered newborns, and 9 of 13 (69%) of low birth weight infants had SPF in their cord serum. Serial studies in 14 infants indicate that SPF disappeared from the serum within 24 hours with a half-life of 3 to 6 hours. The presence of SPF in the serum of newborn infants after 24 hours (found in 7 of 11 sick infants in this study) suggests a pathologic condition associated with fibrin breakdown such as sepsis, respiratory distress, or internal bleeding. Cord serum SPF levels were not correlated with levels of other coagulation factors, nor with clinical features such as length of labor. Since SPF in cord serum are not increased in traumatic delivery, we conclude that their origin is the continuous lysis of clots within the placenta. SPF were also present in 23 of 77 (30%) maternal sera obtained immediately after delivery. There was no correlation between maternal and cord serum SPF levels and their pattern differed on immunoelectrophoresis. Maternal SPF levels appear during labor and disapppear shortly after delivery.


1984 ◽  
Vol 15 (1) ◽  
pp. 27-33
Author(s):  
Cleide Enoir ◽  
Petean Trindade† ◽  
Maria Eneida ◽  
Aiello Sartor† ◽  
Fernando Jose de Nobrega ◽  
...  

2013 ◽  
Vol 2013 ◽  
pp. 1-4 ◽  
Author(s):  
Hye Ryun Lee ◽  
Jeong Su Park ◽  
Sue Shin ◽  
Eun Youn Roh ◽  
Jong Hyun Yoon ◽  
...  

We evaluated the relationship between mean platelet volume (MPV) and characteristics of 10,577 cord blood (CB) units in a public CB bank in Korea. Blood group O has the highest MPV (P= 0.002). MPV correlated with CB volume (r=0.121), Hb (r=0.377), WBC (r=0.111), TNCs (r=0.110), CD34+ cell (r=0.174), CD34+ cells/TNCs (r=0.157), gestational age (r= −0.102), and birth weight (r=0.023); (P<0.001in all). MPV may be one of the useful decision parameters of process priority in CB bank.


1989 ◽  
Vol 26 (2) ◽  
pp. 106-108 ◽  
Author(s):  
Lars Irestedt ◽  
Ingrid Dahlin ◽  
Torbjörn Hertzberg ◽  
Alf Sollevi ◽  
Hugo Lagercrantz

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