Dietary omega-3 fatty acids lower levels of platelet-derived growth factor mRNA in human mononuclear cells

Platelet-derived growth factor (PDGF) is a potent mitogen thought to propagate atherosclerosis and other proliferative or inflammatory diseases. Some of these diseases are ameliorated in humans by ingestion of omega-3 fatty acids. We investigated mRNA expression of both PDGF-A and PDGF-B in quiescent peripheral blood mononuclear cells from healthy male volunteers. For this, a highly sensitive, quantitative polymerase chain reaction strategy (3n-PCR) was developed. In contrast to granulocytes, both PDGF-A and PDGF-B mRNAs are expressed in mononuclear cells. This expression occurs at a remarkably constant rate. Moreover, effects of 7 g/d of a 85% omega-3 fatty acid fish oil concentrate were investigated in a 6-week controlled, randomized, observer-blind study in 14 human volunteers, 7 of whom served as controls. omega-3 Fatty acids increased in mononuclear cell phospholipids. We demonstrate for the first time that diet affects human gene regulation. Dietary omega-3 fatty acids downregulate gene expression of both PDGF-A (-66%), and PDGF-B (-70%). This may represent a novel mechanism for the antifibrotic and antiatherosclerotic action of omega-3 fatty acids.

Download Full-text

Dietary omega-3 fatty acids lower levels of platelet-derived growth factor mRNA in human mononuclear cells

Blood ◽

10.1182/blood.v81.7.1871.1871 ◽

1993 ◽

Vol 81 (7) ◽

pp. 1871-1879 ◽

Cited By ~ 91

Author(s):

WE Kaminski ◽

E Jendraschak ◽

R Kiefl ◽

C von Schacky

Keyword(s):

Fatty Acids ◽

Growth Factor ◽

Mononuclear Cells ◽

Inflammatory Diseases ◽

Quantitative Polymerase Chain Reaction ◽

Platelet Derived Growth Factor ◽

Omega 3 Fatty Acids ◽

Omega 3 ◽

Peripheral Blood Mononuclear ◽

Human Mononuclear Cells

Abstract Platelet-derived growth factor (PDGF) is a potent mitogen thought to propagate atherosclerosis and other proliferative or inflammatory diseases. Some of these diseases are ameliorated in humans by ingestion of omega-3 fatty acids. We investigated mRNA expression of both PDGF-A and PDGF-B in quiescent peripheral blood mononuclear cells from healthy male volunteers. For this, a highly sensitive, quantitative polymerase chain reaction strategy (3n-PCR) was developed. In contrast to granulocytes, both PDGF-A and PDGF-B mRNAs are expressed in mononuclear cells. This expression occurs at a remarkably constant rate. Moreover, effects of 7 g/d of a 85% omega-3 fatty acid fish oil concentrate were investigated in a 6-week controlled, randomized, observer-blind study in 14 human volunteers, 7 of whom served as controls. omega-3 Fatty acids increased in mononuclear cell phospholipids. We demonstrate for the first time that diet affects human gene regulation. Dietary omega-3 fatty acids downregulate gene expression of both PDGF-A (-66%), and PDGF-B (-70%). This may represent a novel mechanism for the antifibrotic and antiatherosclerotic action of omega-3 fatty acids.

Download Full-text

The Effects of Omega-3 Fatty Acids Supplementation on Gene Expression Involved in the Insulin and Lipid Signaling Pathway in Patients with Polycystic Ovary Syndrome

Hormone and Metabolic Research ◽

10.1055/s-0042-122782 ◽

2017 ◽

Vol 49 (06) ◽

pp. 446-451 ◽

Cited By ~ 10

Author(s):

Khadijeh Nasri ◽

Sedigheh Hantoushzadeh ◽

Esmat Aghadavod ◽

Mohsen Taghizadeh ◽

Zatollah Asemi

Keyword(s):

Gene Expression ◽

Fatty Acids ◽

Signaling Pathway ◽

Peripheral Blood Mononuclear Cells ◽

Peripheral Blood ◽

Mononuclear Cells ◽

Lipid Signaling ◽

Omega 3 Fatty Acids ◽

Omega 3 ◽

Peripheral Blood Mononuclear

AbstractLimited data are available evaluating the effects of omega-3 fatty acids supplementation on gene expression involved in the insulin and lipid-signaling pathway in women with polycystic ovary syndrome (PCOS). This study was conducted to evaluate the effects of omega-3 fatty acids supplementation on gene expression involved in the insulin and lipid signaling pathway in women with PCOS. This randomized double blind, placebo-controlled trial was done among 60 women aged 18–40 years old and diagnosed with PCOS according to the Rotterdam criteria. Participants were randomly assigned into 2 groups to receive either 1 000 mg omega-3 fatty acids from flaxseed oil containing 400 mg α-linolenic acid (n=30) or placebo (n=30) twice a day for 12 weeks. Gene expressions involved in the insulin and lipid-signaling pathway were quantified in blood samples of PCOS women with RT-PCR method. Quantitative results of RT-PCR demonstrated that compared with the placebo, omega-3 fatty acids supplementation upregulated peroxisome proliferator-activated receptor gamma (PPAR-γ) mRNA (p=0.005) in peripheral blood mononuclear cells of women with PCOS. In addition, compared to the placebo, omega-3 fatty acids supplementation downregulated expressed levels of oxidized low-density lipoprotein receptor (LDLR) mRNA (p=0.002) in peripheral blood mononuclear cells of women with PCOS. We did not observe any significant effect of omega-3 fatty acids supplementation on expressed levels of glucose transporter 1 (GLUT-1) and lipoprotein(a) [Lp(a)] genes in peripheral blood mononuclear cells. Overall, omega-3 fatty acids supplementation for 12 weeks in PCOS women significantly improved gene expression of PPAR-γ and LDLR.

Download Full-text

Acute and chronic omega-3 fatty acids activates Gpr120 in peripheral blood mononuclear cells from nafld and obese patients reducing inflammation

Clinical Nutrition ESPEN ◽

10.1016/j.clnesp.2020.09.748 ◽

2020 ◽

Vol 40 ◽

pp. 654

Author(s):

E. Batista ◽

V. Muñoz ◽

J. Jesus ◽

M. Vasconcelos ◽

J.M. Rocha ◽

...

Keyword(s):

Fatty Acids ◽

Peripheral Blood Mononuclear Cells ◽

Peripheral Blood ◽

Mononuclear Cells ◽

Obese Patients ◽

Omega 3 Fatty Acids ◽

Omega 3 ◽

Peripheral Blood Mononuclear ◽

Blood Mononuclear Cells

Download Full-text

Proteome dataset of peripheral blood mononuclear cells in postpartum dairy cows supplemented with different sources of omega-3 fatty acids

Data in Brief ◽

10.1016/j.dib.2021.107785 ◽

2022 ◽

pp. 107785

Author(s):

Gitit Kra ◽

Nataly Nemes-Navon ◽

Jayasimha Rayalu Daddam ◽

Lilya Livshits ◽

Shamay Jacoby ◽

...

Keyword(s):

Fatty Acids ◽

Dairy Cows ◽

Peripheral Blood Mononuclear Cells ◽

Peripheral Blood ◽

Mononuclear Cells ◽

Omega 3 Fatty Acids ◽

Omega 3 ◽

Peripheral Blood Mononuclear ◽

Blood Mononuclear Cells ◽

Different Sources

Download Full-text

Proteomic analysis of peripheral blood mononuclear cells and inflammatory status in postpartum dairy cows supplemented with different sources of omega-3 fatty acids

Journal of Proteomics ◽

10.1016/j.jprot.2021.104313 ◽

2021 ◽

pp. 104313

Author(s):

Gitit Kra ◽

Nataly Nemes-Navon ◽

Jayasimha Rayalu Daddam ◽

Lilya Livshits ◽

Shamay Jacoby ◽

...

Keyword(s):

Fatty Acids ◽

Dairy Cows ◽

Peripheral Blood Mononuclear Cells ◽

Mononuclear Cells ◽

Omega 3 Fatty Acids ◽

Omega 3 ◽

Peripheral Blood Mononuclear ◽

Inflammatory Status ◽

Blood Mononuclear Cells ◽

Different Sources

Download Full-text

On-line hemodiafiltration modulates atherosclerosis signaling in peripheral lymphomonocytes of hemodialysis patients

Journal of Nephrology ◽

10.1007/s40620-020-00958-z ◽

2021 ◽

Author(s):

Simona Simone ◽

Annarita Chieti ◽

Paola Pontrelli ◽

Federica Rascio ◽

Giuseppe Castellano ◽

...

Keyword(s):

Growth Factor ◽

Gene Networks ◽

Mononuclear Cells ◽

Expression Profiles ◽

Hemodialysis Patients ◽

Monoamine Oxidase A ◽

Platelet Derived Growth Factor ◽

Peripheral Blood Mononuclear ◽

On Line ◽

Factor C

Abstract Background Hemodialysis patients present a dramatic increase in cardiovascular morbidity/mortality. Circulating immune cells, activated by both uremic milieu and dialysis, play a key role in the pathogenesis of dialysis-related vascular disease. The aim of our study was to identify, through a high-throughput approach, differences in gene expression profiles in the peripheral blood mononuclear cells (PBMCs) of patients treated with on-line hemodiafiltration and bicarbonate hemodialysis. Methods The transcriptomic profile was investigated in PBMCs isolated from eight patients on on-line hemodiafiltration and eight patients on bicarbonate hemodialysis by microarray analysis. The results were evaluated by statistical and functional pathway analysis and validated by real time PCR (qPCR) in an independent cohort of patients (on-line hemodiafiltration N = 20, bicarbonate hemodialysis n = 20). Results Eight hundred and forty-seven genes were differentially expressed in patients treated with on-line hemodiafiltration and bicarbonate hemodialysis. Thirty-seven functional gene networks were identified and atherosclerosis signaling was the top canonical pathway regulated by on-line hemodiafiltration. Among the genes of this pathway, on-line hemodiafiltration was associated with a reduced expression of Platelet-derived growth factor A chain (PDGF A), Clusterin, Monoamine Oxidase A, Interleukin-6 (IL-6) and Vascular Endothelial Growth Factor C (VEGF-)C and with an increase of Apolipoprotein E. qPCR confirmed the microarray results. Platelet derived growth factor AA (PDGF-AA), IL-6 and VEGF-C serum levels were significantly lower in the on-line hemodiafiltration group. Finally, 10 patients previously on bicarbonate hemodialysis were switched to on-line hemodiafiltration and PBMCs were harvested after 6 months. The qPCR results from this perspective group confirmed the modulation of atherosclerotic genes observed in the cross-sectional analysis. Conclusions Our data suggest that type of dialysis (on-line hemodiafiltration versus bicarbonate hemodialysis) may modulate the expression of several genes involved in the pathogenesis of atherosclerotic disease.

Download Full-text

Decreased levels of miR-28-5p and miR-361-3p and increased levels of insulin-like growth factor 1 mRNA in mononuclear cells from patients with hereditary hemorrhagic telangiectasia

Canadian Journal of Physiology and Pharmacology ◽

10.1139/cjpp-2018-0508 ◽

2019 ◽

Vol 97 (6) ◽

pp. 562-569 ◽

Cited By ~ 4

Author(s):

Anthony Cannavicci ◽

Qiuwang Zhang ◽

Si-Cheng Dai ◽

Marie E. Faughnan ◽

Michael J.B. Kutryk

Keyword(s):

Growth Factor ◽

Peripheral Blood ◽

Hereditary Hemorrhagic Telangiectasia ◽

Mononuclear Cells ◽

Quantitative Polymerase Chain Reaction ◽

Clinical Manifestations ◽

Mrna Levels ◽

Insulin Like Growth Factor ◽

Peripheral Blood Mononuclear ◽

Micro Rnas

Hereditary hemorrhagic telangiectasia (HHT) is a rare vascular disorder inherited in an autosomal dominant manner. Patients with HHT can develop vascular dysplasias called telangiectasias and arteriovenous malformations (AVMs). Our objective was to profile and characterize micro-RNAs (miRNAs), short noncoding RNAs that regulate gene expression posttranscriptionally, in HHT patient-derived peripheral blood mononuclear cells (PBMCs). PBMCs, comprised mostly of lymphocytes and monocytes, have been reported to be dysfunctional in HHT. A total of 40 clinically confirmed HHT patients and 22 controls were enrolled in this study. PBMCs were isolated from 16 mL of peripheral blood and purified for total RNA. MiRNA expression profiling was conducted with a human miRNA array analysis. Select dysregulated miRNAs and miRNA targets were validated with reverse transcription–quantitative polymerase chain reaction. Of the 377 miRNAs screened, 41 dysregulated miRNAs were identified. Both miR-28-5p and miR-361-3p, known to target insulin-like growth factor 1 (IGF1), a potent angiogenic growth factor, were found to be significantly downregulated in HHT patients. Consequently, IGF1 mRNA levels were found to be significantly elevated. Our research successfully identified miRNA dysregulation and elevated IGF1 mRNA levels in PBMCs from HHT patients. This novel discovery represents a potential pathogenic mechanism that could be targeted to alleviate clinical manifestations of HHT.

Download Full-text