The novel myokine irisin interacts with hyaluronic acid in a cell-specific way and in association with COPD progression and severity

2016 ◽  
Author(s):  
Eleni Papakonstantinou ◽  
Michael Roth ◽  
Jyotshna Mandal ◽  
Michael Tamm ◽  
Daiana Stolz
Keyword(s):  
Hyaluronic Acid ◽  
The Novel ◽  
A Cell

scholarly journals Modeling and Simulation of Thermo-Fluid-Electrochemical Ion Flow in Biological Channels

2015 ◽  
Vol 3 (1) ◽  
Author(s):  
Riccardo Sacco ◽  
Fabio Manganini ◽  
Joseph W. Jerome
Keyword(s):  
Driving Forces ◽  
The Novel ◽  
Thermodynamical Equilibrium ◽  
Finite Element Scheme ◽  
Equilibrium Conditions ◽  
Solution Map ◽  
Ion Flow ◽  
Stabilized Finite Element ◽  
A Cell ◽  
Biological Channels

AbstractIn this articlewe address the study of ion charge transport in the biological channels separating the intra and extracellular regions of a cell. The focus of the investigation is devoted to including thermal driving forces in the well-known velocity-extended Poisson-Nernst-Planck (vPNP) electrodiffusion model. Two extensions of the vPNP system are proposed: the velocity-extended Thermo-Hydrodynamic model (vTHD) and the velocity-extended Electro-Thermal model (vET). Both formulations are based on the principles of conservation of mass, momentum and energy, and collapse into the vPNP model under thermodynamical equilibrium conditions. Upon introducing a suitable one-dimensional geometrical representation of the channel,we discuss appropriate boundary conditions that depend only on effectively accessible measurable quantities. Then, we describe the novel models, the solution map used to iteratively solve them, and the mixed-hybrid flux-conservative stabilized finite element scheme used to discretize the linearized equations. Finally,we successfully apply our computational algorithms to the simulation of two different realistic biological channels: 1) the Gramicidin-A channel considered in [12]; and 2) the bipolar nanofluidic diode considered in [45].

scholarly journals Evaluation of the Rheologic and Physicochemical Properties of a Novel Hyaluronic Acid Filler Range with eXcellent Three-Dimensional Reticulation (XTR™) Technology

Polymers ◽  
2020 ◽  
Vol 12 (8) ◽  
pp. 1644
Author(s):  
Giovanni Salti ◽  
Salvatore Piero Fundarò
Keyword(s):  
Hyaluronic Acid ◽  
Research Laboratory ◽  
Three Dimensional ◽  
Size Distributions ◽  
The Novel ◽  
Independent Research ◽  
Aesthetic Medicine ◽  
Dimensional Network ◽  
Pore Size Distributions ◽  
Patient Profiles

Soft-tissue fillers made of hyaluronic acid and combined with lidocaine have recently become a popular tool in aesthetic medicine. Several manufacturers have developed their own proprietary formulae with varying manufacturing tools, concentrations, crosslinked three-dimensional network structures, pore size distributions of the fibrous networks, as well as cohesivity levels and rheological properties, lending fillers and filler ranges their unique properties and degradability profiles. One such range of hyaluronic acid fillers manufactured using the novel eXcellent three-dimensional reticulation (XTR™) technology was evaluated in comparison with other HA fillers and filler ranges by an independent research laboratory. Fillers manufactured with the XTR™ technology were shown to have characteristic rheological, crosslinking and biophysical factors that support the suitability of this filler range for certain patient profiles.

Laryngeal Electromyography-Guided Hyaluronic Acid Vocal Fold Injections for Glottic Insufficiency

2020 ◽  
Vol 129 (11) ◽  
pp. 1063-1070
Author(s):  
Alice Q. Liu ◽  
Joel Singer ◽  
Terry Lee ◽  
Amanda Hu
Keyword(s):  
Hyaluronic Acid ◽  
Fundamental Frequency ◽  
Vocal Fold ◽  
The Novel ◽  
Completion Rates ◽  
Laryngeal Electromyography ◽  
Glottic Insufficiency ◽  
Maximum Phonation Time ◽  
Novel Technique ◽  
Glottal Insufficiency

Objectives: To assess voice outcomes using the novel technique of in-office laryngeal electromyography-guided vocal fold injections (LEVFI) with hyaluronic acid to treat glottal insufficiency. Secondary objectives included determining the complication/completion rates and if any factors were associated with improved voice outcomes. Methods: Retrospective review of patients who received their first LEVFI from August 2017 to December 2018. Three- and six-month voice outcomes were assessed. Outcomes included voice handicap index-10 (VHI-10), maximum phonation time (MPT), perceptual analysis of voice (GRBAS), fundamental frequency, and stroboscopy. Results: Of the 121 eligible patients (55.4% male, age 63.7 years), 94 (77.7%) had complete 3-month data and 59 (48.8%) had complete 6-month data. VHI-10 was significantly improved from 25.7 ± 7.5 to 20.9 ± 10.9 at 3 months ( P < .001) and to 19.1 ± 11.5 at 6 months ( P < .001). MPT improved from 6.2 ± 5.4 seconds to 9.4 ± 7.1 seconds at 3 months ( P < .001) and to 11.3 ± 8.2 seconds at 6 months ( P < .001). GRBAS was improved in 74.8% of patients ([65.2, 82.8] 95% CI) at 3 months and 80.8% ([69.9, 89.1]) 95% CI) at 6 months. Stroboscopy showed a glottic gap improvement in 74.8% of patients ([65.8, 82.4] 95% CI) at 3 months and in 80.3% ([65.9, 88.5] 95% CI) at 6 months. Fundamental frequency was unchanged, as expected. Multivariate analysis reported that no factors were associated with better voice outcomes. Overall, 177/181 (97.8%) injections were completed. There were no complications. Conclusion: In-office LEVFI is an effective, novel technique to treat glottic insufficiency with improved voice outcomes, high completion rate, and no significant complications.

Potential of Fortified Fibrin/Hyaluronic Acid Composite Gel as a Cell Delivery Vehicle for Chondrocytes

2009 ◽  
Vol 33 (6) ◽  
pp. 439-447 ◽  
Author(s):  
Sang-Hyug Park ◽  
Ji Hao Cui ◽  
So Ra Park ◽  
Byoung-Hyun Min
Keyword(s):  
Hyaluronic Acid ◽  
Cell Delivery ◽  
Delivery Vehicle ◽  
Composite Gel ◽  
A Cell

Enhanced radiation effect on tumor growth using oxygen-rich microcapsules

2013 ◽  
Vol 23 (01n02) ◽  
pp. 39-45
Author(s):  
S. Harada ◽  
S. Ehara ◽  
K. Ishii ◽  
T. Sato ◽  
M. Koka ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Hyaluronic Acid ◽  
Cancer Cells ◽  
Human Breast Cancer ◽  
Oxygen Radicals ◽  
Radiation Effect ◽  
Radiation Treatment ◽  
Human Breast ◽  
A Cell ◽  
Γ Ray

The aim of this study was to determine whether oxygen-releasing microcapsules could be used to sensitize cancer cells to kill by radiation. The microcapsules were generated by spraying a mixture of 0.1% alginate and hyaluronic acid into a 0.3 mmol/l solution of CaCl2 and FeCl2. These were then subcutaneously injected around a MM48 tumor (a cell line derived from human breast cancer) in the left hind legs of C3H/HeN mice, and tumors were dosed with 60Co γ-ray radiation. We showed that the oxygen released from the microcapsules enhanced the anti-tumor effect of radiation treatment via the generation of oxygen radicals.

scholarly journals Hyaluronic Acid-Conjugated Carbon Nanomaterials for Enhanced Tumour Targeting Ability

Molecules ◽  
2021 ◽  
Vol 27 (1) ◽  
pp. 48
Author(s):  
Oisin Kearns ◽  
Adalberto Camisasca ◽  
Silvia Giordani
Keyword(s):  
Drug Delivery ◽  
Hyaluronic Acid ◽  
Cancer Therapy ◽  
Photothermal Therapy ◽  
Near Infrared ◽  
Carbon Nanomaterials ◽  
Graphene Quantum Dots ◽  
Tumour Volume ◽  
The Novel ◽  
Targeting Ability

Hyaluronic acid (HA) has been implemented for chemo and photothermal therapy to target tumour cells overexpressing the CD44+ receptor. HA-targeting hybrid systems allows carbon nanomaterial (CNM) carriers to efficiently deliver anticancer drugs, such as doxorubicin and gemcitabine, to the tumour sites. Carbon nanotubes (CNTs), graphene, graphene oxide (GO), and graphene quantum dots (GQDs) are grouped for a detailed review of the novel nanocomposites for cancer therapy. Some CNMs proved to be more successful than others in terms of stability and effectiveness at removing relative tumour volume. While the literature has been focused primarily on the CNTs and GO, other CNMs such as carbon nano-onions (CNOs) proved quite promising for targeted drug delivery using HA. Near-infrared laser photoablation is also reviewed as a primary method of cancer therapy—it can be used alone or in conjunction with chemotherapy to achieve promising chemo-photothermal therapy protocols. This review aims to give a background into HA and why it is a successful cancer-targeting component of current CNM-based drug delivery systems.

scholarly journals A Cell-Based Biosensor System for Listeria monocytogenes Detection in Food

Proceedings ◽  
2020 ◽  
Vol 60 (1) ◽  
pp. 49
Author(s):  
Agni Hadjilouka ◽  
Konstantinos Loizou ◽  
Theofylaktos Apostolou ◽  
Lazaros Dougiakis ◽  
Antonios Inglezakis ◽  
...  
Keyword(s):  
Listeria Monocytogenes ◽  
Limit Of Detection ◽  
Food Contamination ◽  
Food Samples ◽  
The Novel ◽  
Food Borne ◽  
Biosensor System ◽  
A Cell ◽  
Food Substrate

Listeria monocytogenes is an intracellular bacterium that causes serious epidemic and sporadic food-borne illnesses in humans. Rapid and trustworthy methods are necessary for the detection of the pathogen to prevent potential food contamination. The aim of this study was to test a newly developed L. monocytogenes biosensor on actual food samples and validate its ability to detect the presence of pathogens robustly and accurately. The newly developed method uses a cell-based biosensor technology (BERA) and a portable device developed by EMBIO Diagnostics called B.EL.D, and provides results within 3 min. Tests were conducted on ready-to-eat lettuce salads, milk and halloumi cheese and the results indicate that the novel system was able to identify inoculated samples with 98%, 90%, and 91% accuracy, respectively. Furthermore, the limit of detection was determined to be as low as 0.6 log CFU mL−1 or g−1 in all food types. Classification of the samples Above or Below the detection limit was accessed through a newly developed algorithm for each food substrate. Samples were also analyzed with the ISO 11290-1:2017 and 11290-2:2017, in parallel. Thus, it was concluded that the newly developed biosensor can be a useful tool in the food supply chain, decreasing the required time for the detection of pathogens and increasing the number of tested samples before they reach the market.

Nonsurgical Rhinoplasty With the Novel Hyaluronic Acid Filler VYC-25L: Results Using a Nasal Grid Approach

2020 ◽  
Author(s):  
Dario Bertossi ◽  
Luciano Malchiodi ◽  
Massimo Albanese ◽  
Riccardo Nocini ◽  
Pierfrancesco Nocini
Keyword(s):  
Hyaluronic Acid ◽  
The Novel ◽  
Level Of Evidence ◽  
Acid Product ◽  
Nasolabial Angle ◽  
Independent Evaluation ◽  
Injection Quantity ◽  
Grid Approach ◽  
High Degree

Abstract Background Nonsurgical aesthetic treatment of the nose is becoming increasingly popular. VYC-25L is a novel hyaluronic acid product with the high G’ and cohesivity required of a nasal filler. Objectives The authors sought to assess the safety and efficacy of VYC-25L for treatment of the nose utilizing a previously published, grid-based protocol. Methods This was a retrospective, single-center analysis of data from adult patients undergoing treatment of the nose with VYC-25L between February and April 2019 utilizing the grid system as the reference for injection quantity and sequencing. Specific procedures included correction of inadequate projection, deep glabella treatment, correction of a nasal hump, and adjustment of the nasolabial angle and columella. Patients were followed-up for 6 to 9 months. Results A total of 61 patients were included in the analysis (mean age, 32 ± 3 years; n = 45 females [74%]). At 2 weeks posttreatment, a high degree of defect correction was confirmed based on independent evaluation, with all patients scoring 9 or 10 on a 10-point visual analog scale. Fifty-nine of 61 patients (97%) self-assessed the degree of correction as “adequate.” Results were stable at 3- and 6-month follow-up visits. Complications recorded were bruising (n = 15, 25%), asymmetry (n = 2, 3%), and hematoma (n = 1, 2%). All resolved rapidly. There were no cases of infection, bumps, or skin necrosis. Conclusions VYC-25L is safe and efficacious for treatment of the nose, with high levels of patient satisfaction. It has potential to be a valuable tool in nonsurgical rhinoplasty. Level of Evidence: 4

Reinforcement Fibrin-Hyaluronic Acid Composite Gel for Tissue Engineering Cartilage Genesis

2007 ◽  
Vol 342-343 ◽  
pp. 153-156 ◽  
Author(s):  
Sang Hyug Park ◽  
So Ra Park ◽  
Byoung Hyun Min
Keyword(s):  
Hyaluronic Acid ◽  
Structural Integrity ◽  
Cell Delivery ◽  
Fibrin Gel ◽  
External Compression ◽  
Composite Gels ◽  
Composite Gel ◽  
A Cell ◽  
Safranin O ◽  
Hybrid Biomaterials

The reimplantation method of cultured chondrocytes broadly has been offered as an alternative for articular cartilage repair. A variety of biologically derived and synthetic polymeric and hydrogel materials also have been investigated for good cell delivery efficiency. Preciously, we examined the feasibility of fibrin gel, mixed with hyaluronic acid(HA) as a cell delivery carrier. In order to reinforce the material, hybrid biomaterials of fibrin/HA composite gels with fibrinolysis inhibition factors(FIFs: aprotinin, DI101, EACA) have been investigated in the present work because we did not satisfy a little progress. These fibrin/HA composite gels added FIFs maintained their structural integrity in long-term culture over 4th weeks. Contrary to our expectation the mass of the fibrin/HA composite with DI 101 was significantly superior to the ones of other combinations. In histological evidence, all of them are showed good positive result of stain of Safranin-O and alcian blue during the culture period. In gross examination, samples of all groups grossly resembled cartilage in color and were resistant to external compression. Our study demonstrates that most favorable polymer can be used good quality tissue engineered cartilage and in this culture systems have been useful for studying the basic biology of chondrocyte biosynthesis of ECM and new cartilage matrix formation without a loss of volume. After all, we proved the safety of inhibitors of the fibrinolytic system without hazardous effect on cell behavior and found out that DI 101 would be the most effective agent.

scholarly journals Poly (I:C) and hyaluronic acid directly interact with NLRP3, resulting in the assembly of NLRP3 and ASC in a cell-free system

2017 ◽  
Vol 15 (2) ◽  
pp. 85-97 ◽  
Author(s):  
Naoe Kaneko ◽  
Yuki Ito ◽  
Tomoyuki Iwasaki ◽  
Hiroyuki Takeda ◽  
Tatsuya Sawasaki ◽  
...  
Keyword(s):  
Hyaluronic Acid ◽  
Nlrp3 Inflammasome ◽  
Poly I:C ◽  
Free System ◽  
Cell Free System ◽  
Caspase 1 ◽  
Pyrin Domain ◽  
A Cell ◽  
Homogeneous Assay ◽  
Endogenous Metabolites

In the NLR family, pyrin domain containing 3 (NLRP3) is an intracellular pattern recognition receptor that activates pro-caspase-1, leading to IL-1β and IL-18 processing and activation in a large complex called the NLRP3 inflammasome. Since various pathogens or endogenous metabolites have been reported to stimulate NLRP3 inflammasome, the interaction between NLRP3 and ASC induced by these stimulants may be an attractive drug target for NLRP3-related diseases, called inflammasomopathies. However, the endogenous ligand that directly interacts with NLRP3, leading to binding to ASC, remains unclear. Therefore, we developed a cell-free system consisting of NLRP3, ASC, and pro-caspase-1 or ASC and NLRP3 with an amplified luminescent proximity homogeneous assay (ALPHA). ALPHA signals of the interaction between NLRP3 and ASC were not enhanced following an incubation without any ligand, whereas strong ALPHA signals for the interaction between NLRP3 and ASC and between NLRP3 and pro-caspase-1 with the adaptor ASC were observed upon an incubation with poly (I:C) and hyaluronic acid (HA). Poly (I:C) and HA both directly interacted with NLRP3 within a specific concentration. These results suggest that NLRP3 directly interacts with intrinsic RNA and HA, which is followed by the activation of NLRP3 inflammasome, and the cell-free system consisting of NLRP3 and ASC, or NLRP3, ASC, and pro-caspase-1 may be a useful tool for elucidating the pathogenesis of inflammasomopathies and developing target therapeutics.

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