Evaluation of potential role of vitamin C in differential skeletal development between female and male mice using a mouse model

AbstractThere are limited proven therapeutic options for the prevention and treatment of COVID-19. We underwent an observational study with the aim of measure plasma vitamin C levels in a population of critically ill COVID-19 adult patients who met ARDS criteria according to the Berlin definition. This epidemiological study brings to light that up to 82% had low Vitamin C values. Notwithstanding the limitation that this is a single-center study, it nevertheless shows an important issue. Given the potential role of vitamin C in sepsis and ARDS, there is gathering interest of whether supplementation could be beneficial in COVID-19.

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Potential Role of Vitamins and Zinc on Acute Respiratory Infections Including Covid-19

Global Pediatric Health ◽

10.1177/2333794x211021739 ◽

2021 ◽

Vol 8 ◽

pp. 2333794X2110217

Author(s):

Indah K. Murni ◽

Endy P. Prawirohartono ◽

Rina Triasih

Keyword(s):

Vitamin A ◽

Vitamin C ◽

Large Dose ◽

Common Cold ◽

Potential Role ◽

Respiratory Infections ◽

Acute Respiratory Infections ◽

Under Five ◽

Short Period

Background. Vitamin C, E, D, A, zinc are considered to be essential in preventing and treating of acute respiratory infections (ARI) including COVID-19. Methods. We reviewed published studies evaluating the potential roles of these vitamin and zinc for ARIs and COVID-19 using Medline database, medRxiv, and bibliographic references. Results. Vitamins C, D, and E did not reduce incidence of common cold in general, but vitamin C reduced by half in population with physical and environment stresses. Vitamins C and E shortened duration and reduced severity of common cold. A large-dose vitamin A had no effect on recovery from pneumonia. Zinc improved clinical deterioration and pneumonia duration in under five. The effect on preventing COVID-19 morbidity and related-death was lacking. Conclusions. Although the effects of vitamins and zinc on ARIs including COVID-19 were inconclusive, taking these for a short period during pandemic may be beneficial when there is risks of deficiency.

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Transient sex-related changes in the mice hypothalamo–pituitary–adrenal (HPA) axis during the acute phase of the inflammatory process

Mediators of Inflammation ◽

10.1155/s0962935193000183 ◽

1993 ◽

Vol 2 (2) ◽

pp. 123-127 ◽

Cited By ~ 12

Author(s):

T. Daneva ◽

E. Spinedi ◽

R. Hadid ◽

M.-C. Jacquier ◽

M. Giacomini ◽

...

Keyword(s):

Sex Difference ◽

Acute Phase ◽

Hpa Axis ◽

Plasma Levels ◽

Potential Role ◽

Post Treatment ◽

Male Mice ◽

Plasma Acth ◽

Endogenous Sex Hormones

The potential role of endogenous sex hormones in regulating hypothalamo–pituitary–adrenal (HPA) axis function was investigated after a single injection of endotoxin in adult (8 week old) BALB/c mice of both sexes. The effect of LPS on plasma ACTH, corticosterone (B), testosterone and oestradiol (E) levels and on anterior pituitary (AP) ACTH and adrenal B contents at different times after treatment was studied. The results indicate that: (a) basal B but not ACTH plasma levels were significantly higher in female than in male mice; (b) LPS significantly increased both ACTH and B plasma levels over the baseline 2 h after injection, both hormone levels being higher in female than in male mice; (c) although plasma ACTH concentrations recovered the basal value at 72 h after LPS in animals of both sexes, plasma B levels returned to the baseline only at 120 h after treatment; (d) E plasma levels significantly increased 2 h after LPS and returned to the baseline at 72 h post-treatment, in both sexes; (e) at 2 h after LPS, testosterone plasma levels significantly decreased in male mice and increased in female mice, recovering the baseline level at 120 and 72 h after LPS, respectively; (f) AP ACTH content was similar in both sexes in basal condition and it was significantly diminished 72 h post-treatment without sex difference; whereas AP ACTH returned to basal content 120 h after LPS in males, it remained significantly decreased in females; (g) basal adrenal B content was higher in female than in male mice, and it significantly increased in both sexes 2 h post-LPS, maintaining this sex difference. Whereas adrenal B returned to basal content 72 h after treatment in male mice, it remained significantly enhanced up to 120 h post-LPS in female animals. The data demonstrate the existence of a clear sexual dimorphism in basal condition and during the acute phase response as well as in the recovery of the HPA axis function shortly after infection.

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Potential Role of Sox9 in Patterning Tracheal Cartilage Ring Formation in an Embryonic Mouse Model

Archives of Otolaryngology - Head and Neck Surgery ◽

10.1001/archotol.130.6.732 ◽

2004 ◽

Vol 130 (6) ◽

pp. 732 ◽

Cited By ~ 21

Author(s):

Ravindhra G. Elluru ◽

Jeffrey A. Whitsett

Keyword(s):

Mouse Model ◽

Potential Role ◽

Ring Formation ◽

Embryonic Mouse ◽

Tracheal Cartilage

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Methylation‐dependent control of HIF‐1/VEGF axis in a mouse model of oxygen‐induced retinopathy. Potential role of the glial water channel Aquaporin‐4

Acta Ophthalmologica ◽

10.1111/aos.13972_360 ◽

2018 ◽

Vol 96 (S261) ◽

pp. 96-96

Keyword(s):

Mouse Model ◽

Potential Role ◽

Water Channel ◽

Aquaporin 4 ◽

Oxygen Induced Retinopathy

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Abstract WP308: Role of Microthrombi and Vasospasm in the Development of Delayed Neurological Deficits After Subarachnoid Hemorrhage in Mice

Stroke ◽

10.1161/str.51.suppl_1.wp308 ◽

2020 ◽

Vol 51 (Suppl_1) ◽

Author(s):

Devin W McBride ◽

Ari Dienel ◽

Remya A Veettil ◽

Kanako Matsumura ◽

Peeyush Kumar T. ◽

...

Keyword(s):

Subarachnoid Hemorrhage ◽

Mouse Model ◽

Experimental Studies ◽

Brain Slices ◽

Neurological Deficits ◽

Male Mice ◽

Male And Female ◽

Delayed Neurological Deterioration ◽

First Time

Rationale: Microthrombosis has been suggested as a major factor contributing to delayed neurological deterioration in patients after subarachnoid hemorrhage (SAH). However, experimental studies on the role of microthrombi in delayed deficits after SAH has not been investigated. Our hypothesis is that, following SAH, mice which develop delayed neurological deficits have a greater number of microthrombi than mice which do not develop delayed neurological deficits. Methods: SAH was induced in adult male and female C57BL/6 mice via endovascular perforation. Mice were randomly assigned into sham (n=6/sex) or SAH groups (n=22-24/sex). Neurobehavior was performed on days 1-3, 5, and 7 post-SAH using a composite neuroscore. Animals were sacrificed on the day of delayed deficits or 7 days post-SAH. Microthrombi count and vessel diameters (for vasospasm) were measured using H&E stained brain slices. All outcomes were performed and all data were analyzed by a blinded investigator. Results: Seventeen percent (4/24) of male mice and thirty-six percent (8/22) of female mice developed delayed deficits on days 3-5 post-SAH (Figures 1A and 1B). Those mice which developed delayed deficits had significantly more microthrombi in their brains than mice which did not develop delayed deficits; vasospasm did not correlate with delayed deficits. Additionally, female SAH mice develop delayed deficits at a higher frequency than males (Figure 1C). Conclusions: This work shows for the first time delayed deficits in a SAH mouse model. Further, microthrombi correlated with delayed deficits, whereas no correlation was between delayed deficits and vasospasm. The data within this study suggests that preventing microthrombi may improve functional recovery and reduce the risk of delayed deficits.

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