scholarly journals Laboratory and field studies on the use of artificial mussels as a monitoring tool of platinum exposure in the freshwater environment

2021 ◽  
Vol 33 (1) ◽  
Author(s):  
Marelize Labuschagne ◽  
Sonja Zimmermann ◽  
Nico J. Smit ◽  
Johannes H. Erasmus ◽  
Milen Nachev ◽  
...  
Keyword(s):  
Clarias Gariepinus ◽  
Exposure Period ◽  
Mining Area ◽  
Freshwater Ecosystem ◽  
Exposure Concentration ◽  
Sampling Device ◽  
Freshwater Environment ◽  
Permeable Membrane ◽  
Chelex 100 ◽  
Water Hyacinths

Abstract Background The artificial mussel (AM) is a passive sampling device that was originally developed for monitoring metal concentrations in the marine environment, but is also increasingly used in freshwater environments. The AM consists of a non-permeable Perspex tube, which is closed on both sides with a semi-permeable membrane. The space in between contains Chelex-100 beads, which bind metals. The AM allows the determination of the dissolved, bioaccessible metal fraction in water bodies without killing organisms, as well as environments with unfavorable conditions for living bioindicators. In the present study, the use of the AM was adapted for the monitoring of platinum (Pt) in a freshwater ecosystem. Results The elution of Pt from the Chelex-100 beads was optimized. Two modifications to the original method for the use of AMs are recommended, i.e., washing and separation of the beads through centrifugation and elution with a mixture of 4.5 mL HNO3 and 0.5 mL HCl for approximately 2–3 h to ensure the release of all Pt bound to the beads. Additionally, the uptake kinetics of the AM were determined under laboratory conditions over a wide exposure concentration range (0.1–1000 µg/L) showing highly correlated Pt accumulation in the AMs with the aqueous exposure concentration. For the tested Pt exposure concentrations of 0.1, 1, 10, and 100 µg/L, the Pt concentrations in the AMs increased during the exposure period of 6 weeks. At the highest exposure concentration of 1000 µg/L, the increase stagnated after 3 weeks. To validate the AM in the field, the Pt accumulation of the AM was assessed together with that of freshwater clams (Corbicula fluminalis africana), muscle and liver tissue of the three fish species sharptooth catfish (Clarias gariepinus), common carp (Cyprinus carpio) and Mozambique tilapia (Oreochromis mossambicus), as well as water hyacinths (Eichhornia crassipes) at two sampling sites in the Pt mining area of South Africa. Conclusion Results from the present study showed that the AM is a promising tool to monitor Pt concentrations in the freshwater environment at contaminated sites.

scholarly journals Laboratory and field studies on the use of artificial mussels as a monitoring tool of platinum exposure in the freshwater environment.

2021 ◽  
Author(s):  
Marelize Labuschagne ◽  
Sonja Zimmermann ◽  
Nico J. Smit ◽  
Johannes H. Erasmus ◽  
Milen Nachev ◽  
...  
Keyword(s):  
Clarias Gariepinus ◽  
Exposure Period ◽  
Mining Area ◽  
Freshwater Ecosystem ◽  
Exposure Concentration ◽  
Sampling Device ◽  
Freshwater Environment ◽  
Permeable Membrane ◽  
Chelex 100 ◽  
Water Hyacinths

Abstract Background The artificial mussel (AM) is a passive sampling device that was originally developed for monitoring metal concentrations in the marine environment but is also increasingly used in freshwater environments. The AM consists of a non-permeable Perspex tube, which is closed on both sides with a semi-permeable membrane. In the space in between are Chelex-100 beads, which bind metals. The AM allows the determination of the dissolved, bioaccessible metal fraction in water bodies without killing organisms, as well as environments with unfavorable conditions for living bioindicators. In the present study, the use of the AM was adapted for the monitoring of platinum (Pt) in a freshwater ecosystem. Results The elution of Pt from the Chelex-100 beads was optimized. Two modifications to the original method for the use of AMs are recommended, i.e. washing and separation of the beads through centrifugation and elution with a mixture of 4.5 mL HNO3 and 0.5 mL HCl for approximately 2-3 hours to ensure the release of all Pt bound to the beads. Additionally, the uptake kinetics of the AM were determined under laboratory conditions over a wide exposure concentration range (0.1-1000 µg/L) showing highly correlated Pt accumulation in the AMs with the aqueous exposure concentration. For the tested Pt exposure concentrations of 0.1, 1, 10, and 100 µg/L, the Pt concentrations in the AMs increased during the exposure period of 6 weeks. At the highest exposure concentration of 1000 µg/L, the increase stagnated after 3 weeks. To validate the AM in the field, the Pt accumulation of the AM was assessed together with that of freshwater clams (Corbicula fluminalis africana), muscle and liver tissue of three fish species sharptooth catfish (Clarias gariepinus), common carp (Cyprinus carpio) and Mozambique tilapia (Oreochromis mossambicus), as well as water hyacinths (Eichhornia crassipes) at two sampling sites in the Pt mining area of South Africa. Conclusion Results from the present study showed that the AM is a promising tool to monitor Pt concentrations in the freshwater environment at contaminated sites.

scholarly journals Laboratory and Field Studies on the Use of Artificial Mussels as a Monitoring Tool of Platinum Exposure in the Freshwater Environment.

2020 ◽  
Author(s):  
Marelize Labuschagne ◽  
Sonja Zimmermann ◽  
Nico J. Smit ◽  
Johannes H. Erasmus ◽  
Milen Nachev ◽  
...  
Keyword(s):  
Clarias Gariepinus ◽  
Exposure Period ◽  
Mining Area ◽  
Original Method ◽  
Freshwater Ecosystem ◽  
Exposure Concentration ◽  
Sampling Device ◽  
Freshwater Environment ◽  
Chelex 100 ◽  
Water Hyacinths

Abstract BackgroundThe artificial mussel (AM) is a passive sampling device that was originally developed for monitoring metal concentrations in the marine environment but is also increasingly used in freshwater environments. The AM consists of a non-permeable Perspex tube, which contains Chelex-100 resin, water diffuses through the semi-permeable membranes where the metals bind to the Chelex-100. The AM allows for the determination of the dissolved, bioaccessible metal fraction in water bodies without killing organisms, as well as environments with unfavorable conditions for living bioindicators. In the present study, the use of the AM was adapted for the monitoring of platinum (Pt) in a freshwater ecosystem. ResultsTherefore, the elution of Pt from the Chelex-100 beads was optimized. Two modifications to the original method for the use of AMs are recommended, i.e. washing and separation of the beads through centrifugation and elution with a mixture of 4.5 mL HNO3 and 0.5 mL HCl for approximately 2-3 hours to ensure the release of all Pt bound to the beads. Additionally, the uptake kinetics of the AM were determined under laboratory conditions over a wide exposure concentration range (0.1-1000 µg/L) showing highly correlated Pt accumulation in the AMs with the aqueous exposure concentration. For the tested Pt exposure concentrations of 0.1, 1, 10, and 100 µg/L, the Pt concentrations in the AMs increased during the exposure period of 6 weeks. At the highest exposure concentration of 1000 µg/L, the increase stagnated after 3 weeks. To validate the AM in the field, the Pt accumulation of the AM was assessed together with that of freshwater clams (Corbicula fluminalis africana), muscle and liver tissue of three fish species sharptooth catfish (Clarias gariepinus), common carp (Cyprinus carpio) and Mozambique tilapia (Oreochromis mossambicus), as well as water hyacinths (Eichhornia crassipes) at two sampling sites in the Pt mining area of South Africa. ConclusionResults from the present study showed that the AM is a promising tool to monitor Pt concentrations in the freshwater environment at contaminated sites.

scholarly journals Effects of Dimethoate Insecticide on Serum Biochemistry of African Catfish (Clarias gariepinus) Juveniles

2021 ◽  
Vol 25 (6) ◽  
pp. 977-985
Author(s):  
O.O. Oghale ◽  
A.C. Okoboshi ◽  
A.P. Chinedu-Ndukwe ◽  
K.C. Kanu ◽  
M. Beka ◽  
...  
Keyword(s):  
Clarias Gariepinus ◽  
Exposure Period ◽  
Serum Biochemistry ◽  
Probit Analysis ◽  
Mortality Data ◽  
African Catfish ◽  
Exposure Concentration ◽  
Lc50 Value ◽  
Run Off ◽  
Static Bioassay

The present study was designed to investigate acute toxicity of  dimethoate on juvenile Clarias gariepinus. Catfish with mean weight and length of 24.01 ± 11.60 g and 13.98 ± 5.66 cm respectively were exposed to sub-lethal levels of dimethoate in static bioassay system for 28 days and mortality data was statistically evaluated using Finney’s Probit analysis. The 96-hour LC50 value for C. gariepinus was estimated as 29.05 mg/l. The value for the LC50 was used in deducing the sub-lethal concentrations (0.01, 0.15and 0.29 mg/l). Biochemical changes in the serum such as glycogen, total protein, cholesterol, urea and creatinine were analyzed after the exposure period (28 days). The glycogenlevel decreased in all sublethal exposures. An increase in cholesterol at lower exposure concentration, followed by slight increase in urea and creatinine among treated groups was recorded. Behavioural changes which includes changes in skin colour, jerking, restlessness, erratic swimming, among others were observed. Based on the result of this study, it was concluded that C. gariepinus juveniles are very sensitive to low concentration of dimethoate. Dimethoate is highly toxic to juvenile catfishes and as such measures must be taken to avoid its run-off into our aquatic environment thus jeopardizing the recruitment of juvenile catfishes into the fishery.

scholarly journals Liver histopathological changes in Clarias gariepinus exposed to ethanol extract of Adenium obesum stem bark

2015 ◽  
Vol 32 (01) ◽  
pp. 022-028 ◽  
Author(s):  
S. Abalaka ◽  
M. Fatihu ◽  
N. Ibrahim ◽  
S. Ambali
Keyword(s):  
Clarias Gariepinus ◽  
Ethanol Extract ◽  
Exposure Period ◽  
Stem Bark ◽  
Aquatic Environments ◽  
Median Lethal Concentration ◽  
Exposed Fish ◽  
Range Finding ◽  
Serum Aspartate Aminotransferase ◽  
Histopathological Effects

Abstract Introduction: Histopathological effects of ethanol extract of Adenium obesum stem bark on the liver of Clariasgariepinus was investigated over a 96-h exposure period as an effective organic piscicide. Materials and Methods: An acute static toxicity bioassay was performed after a preliminary concentration range finding test was conducted. Results: Exposed fish showed adaptation behavioural changes with those of respiratory distress and abnormal nervous signs of toxicity, which was concentration-dependent. Mortality was recorded in some of the exposed fish while a median lethal concentration of 7.15 mgL-1 was established for the extract in the exposed fish. Non-significant (p>0.05) changes were recorded in serum aspartate aminotransferase, alanine aminotransferase and alkaline phosphatase activities of the exposed ish. Moderate liver damage was observed in the exposed fish based on the degree of tissue change (DTC) grading where significant (p<0.05) differences were observed between the DTC of the exposed groups and the control. Conclusion: Ethanol extract of A. obesum stem bark was toxic to the exposed fish with evidence of hepatotoxicity and therefore, can be used to eradicate the hardy C.gariepinus from aquatic environments.

An Overview of Dynamics and Biotoxicity of Metals in the Freshwater Environment

1993 ◽  
Vol 28 (1) ◽  
pp. 1-6 ◽  
Author(s):  
P.M. Huang
Keyword(s):  
Water Column ◽  
Interstitial Water ◽  
Dynamic Equilibrium ◽  
Kinetic Studies ◽  
Integrated Approach ◽  
Freshwater Ecosystem ◽  
Overlying Water ◽  
Freshwater Environment ◽  
Particulate Materials ◽  
The Impact

Abstract The toxic metals, including metalloids, in the freshwater ecosystem are largely associated with surficial sediments and suspended particulate materials. These metals are in dynamic equilibrium with interstitial water and the overlying water column. The bioavailability and toxicity of metals in the freshwater environment are influenced by their speciation and dynamics. Our current understanding of the nature of metal partitioning in particulate materials, interstitial water and the overlying water column is quite limited because of the limitations of the metal fractionation methods and difficulties in obtaining thermodynamic information which approaches the realities in streams, rivers and lakes. Little is known about the in situ metal dynamics. Kinetic studies of metal reactions, thus, warrant in-depth research for years to come. Besides inorganic and organic colloids, microbes contribute to metal transformations. The impact of the interactions of microbes with minerals and organic components on the dynamics and biotoxicity of metals merits attention. Over the last decade, there has been much research on the development of hydrochemical models for better understanding and predicting metal transport in the freshwater system, yet little research has been focused on how well they describe field data. The supply of biologically available metals in the freshwater environment is governed by a series of physical, physicochemical, biochemical and biological processes. To date, there are very few studies on the subject in which an integrated approach has been taken. The roles of these interacting processes in affecting metal dynamics and their impacts on freshwater toxicology deserve increasing attention.

Titanium dioxide nanoparticles-induced cytogenotoxicity and alterations in haematological indices of Clarias gariepinus (Burchell, 1822)

2020 ◽  
Vol 36 (10) ◽  
pp. 807-815
Author(s):  
Opeoluwa M Ogunsuyi ◽  
Elizabeth O Adegoye ◽  
Olusegun I Ogunsuyi ◽  
Okunola A Alabi ◽  
Chibuisi G Alimba ◽  
...  
Keyword(s):  
Titanium Dioxide ◽  
Tap Water ◽  
Clarias Gariepinus ◽  
Haemoglobin Concentration ◽  
Exposure Period ◽  
Negative Control ◽  
African Catfish ◽  
Mean Cell Volume ◽  
Corpuscular Haemoglobin ◽  
Mean Corpuscular Haemoglobin

The application of titanium dioxide (TiO2) nanoparticles (NPs) in the manufacturing of consumer products has increased tremendously and with the potential to induce deleterious effects on aquatic biota. There have been reports on metal oxide NP toxicity in aquatic organisms, however, information on cytotoxicity and genotoxicity of TiO2 NPs on the African catfish, Clarias gariepinus, is scarce. In this study, we investigated the genotoxicity and haematotoxicity of TiO2 NPs in C. gariepinus using the micronucleus (MN) assay and haematological analysis, respectively. Juvenile C. gariepinus were exposed to 6.25, 12.5, 25.0, 50.0 and 100.0 mg L−1 concentrations of TiO2 NPs for 7 and 28 days. Benzene (0.05 mL L−1) and dechlorinated tap water were used as positive and negative controls, respectively. Data of the MN showed a significant ( p < 0.05) concentration-dependent increase in the frequency of MN at both exposure periods in comparison to negative control. Red blood cells, haematocrit, platelets and heterophils significantly reduced with an increased mean corpuscular haemoglobin concentration and lymphocytes at the 7-day exposure period, while in the 28-day exposure period, mean cell volume, mean corpuscular haemoglobin and lymphocytes had a significant increase in comparison with the negative control. This study indicates that TiO2 NPs induced cytogenetic and haematological alterations in C. gariepinus and is of relevance in biodiversity and aquatic health management.

Toxicity of glyphosate-based isopropylamine salt and polyethoxylated tallow amine formulations on juvenile Clarias gariepinus

2021 ◽  
Vol 36 (1) ◽  
pp. 63-73
Author(s):  
A.B. Ella ◽  
E.T. Azua ◽  
C.U. Aguoru ◽  
A.A. Onekutu ◽  
F.A. Ella
Keyword(s):  
Clarias Gariepinus ◽  
Exposure Period ◽  
Quality Parameters ◽  
Fish Mortality ◽  
African Catfish ◽  
Physico Chemical ◽  
Tail Movement ◽  
Glyphosate Formulation ◽  
Lower Limits ◽  
Eventual Death

The formulation of glyphosate composed of Isopropylamine salt and Polyethoxylated tallow Amine (Clearweed) is widely used as herbicide to control weeds both in the terrestrial and aquatic environments. A static bioassay was conducted to examine toxicity of this formulation on juvenile African catfish (Clarias gariepinus). Catfish juvenile (mean weight 27.97±0.03g) were exposed to glyphosate at concentrations of 0.00mg/l (control), 5.00, 7.50, 10.00, 12.50 and 15.00mg/l. Each concentration was treated in triplicate and the exposure period lasted for 96 hrs. Mortality rates and physico-chemical parameters of water were monitored. Results revealed that fish mortality increased with increasing concentration of glyphosate and time of exposure. The median lethal concentration (96-hr LC50) value was 8.88mg/l with the upper and lower limits of 9.10mg/l and 7.75mg/l respectively. Behavioural changes observed the treated fish included: erratic swimming, jerky movement, increased opercula and tail movements, gulping of air, lost of balance and consciousness, cessation of opercula and tail movement signifying eventual death. Water quality parameters increased significantly (p<0.05) with extract concentration except dissolve oxygen levels which reduced (p<0.05). However, all values reported were within the permissible limits of the Federal Ministry of Environment (FMEnv) for water samples. The results of this study indicated that glyphosate formulation has toxic effects on catfish. Thus, the herbicide should be cautiously used to avoid ecotoxicological hazards particularly on non-target organisms.

scholarly journals Phytoremediation of iron in ex-sand mining waters by water hyacinth (Eichhornia crassipes)

2021 ◽  
Vol 22 (2) ◽  
Author(s):  
Qadar Hasani ◽  
NIKEN T.M. PRATIWI ◽  
YUSLI WARDIATNO ◽  
HEFNI EFFENDI ◽  
ARTHO NUGRAHA MARTIN ◽  
...  
Keyword(s):  
Water Quality ◽  
Water Hyacinth ◽  
Water Surface ◽  
Eichhornia Crassipes ◽  
Surface Coverage ◽  
Translocation Factor ◽  
Mining Area ◽  
Sand Mining ◽  
High Concentration ◽  
Water Hyacinths

Hasani Q, Pratiwi NTM, Wardiatno Y, Effendi H, Martin AN, Efendi E, Pirdaus P. 2020. Phytoremediation of Iron (Fe) in Ex-sand Mining Waters by Water Hyacinth (Eichhornia crassipes (Mart.) Solms). The high concentration of iron (Fe) has allegedly caused the water quality of puddles at sand mining area. The poor water quality, in which high contentrations of iron will to a reduction in aquaculture production. This study verified the ability of water hyacinths (Eichhornia crassipes (Mart.) Solms.)) as a phytoremediation agent for iron (Fe) concentration in water at the sand mining area, in East Lampung Regency, Indonesia. The study was carried out with 3 treatments and 3 replication each, including the treatment of (i) 25% water surface coverage, (ii) 50% water surface coverage, and (iii) 75% water surface coverage of water hyacinth. Measurement of Fe concentrations, bioconcentration factor (BCF) and translocation factor (TF) once a week, until Fe concentrations in water are adequate for aquaculture. This was achieved for 21 days. The results showed that the highest percentage reduction of Fe (97.49%) was observed at water hyacinth cover area 50%. The highest value of BCF was 2385.51, while the highest TF was 1.14 in stems and 1.02 in leaves. The results of this study open up opportunities for use water in ex-sand mining areas for aquaculture by the community and its management by the Government of East Lampung Regency, Lampung, Indonesia.

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