scholarly journals RNA-seq analysis reveals the role of red light in resistance against Pseudomonas syringae pv. tomato DC3000 in tomato plants

BMC Genomics ◽  
2015 ◽  
Vol 16 (1) ◽  
pp. 120 ◽  
Author(s):  
You-Xin Yang ◽  
Meng-Meng Wang ◽  
Yan-Ling Yin ◽  
Eugen Onac ◽  
Guo-Fu Zhou ◽  
...  
Keyword(s):  
Pseudomonas Syringae ◽  
Red Light ◽  
Rna Seq ◽  
Tomato Dc3000 ◽  
Tomato Plants

scholarly journals Induction of Pseudomonas syringae pv. tomato DC3000 MexAB-OprM Multidrug Efflux Pump by Flavonoids Is Mediated by the Repressor PmeR

2011 ◽  
Vol 24 (10) ◽  
pp. 1207-1219 ◽  
Author(s):  
Paola Vargas ◽  
Antonia Felipe ◽  
Carmen Michán ◽  
María-Trinidad Gallegos
Keyword(s):  
Pseudomonas Syringae ◽  
Efflux Pump ◽  
Regulatory Gene ◽  
Multidrug Efflux ◽  
Tomato Dc3000 ◽  
Multidrug Efflux Pump ◽  
Tomato Plants ◽  
In Vivo Experiments

In this study, we have analyzed the expression of the Pseudomonas syringae pv. tomato DC3000 mexAB-oprM efflux pump operon and of the regulatory gene pmeR, and we have investigated the role of the PmeR protein on transcription from both promoters. We demonstrate that mexAB-oprM and pmeR are expressed in vivo at a relatively high and moderate basal level, respectively, which, in both cases, increases in the presence of different flavonoids and other compounds, such as butyl and methylparaben. We show that PmeR is the local repressor of the mexAB-oprM promoter and is able to regulate its own expression. The mechanism for this regulation includes binding to a pseudopalindromic operator site which overlaps both mexAB-oprM and pmeR promoters. We have also proven that flavonoids are able to interact with PmeR and induce a conformational change that interferes with the DNA binding ability of PmeR, thereby modulating mexAB-oprM and pmeR expression. Finally, we demonstrate by in vivo experiments that the PmeR/MexAB-OprM system contributes to the colonization of tomato plants. These results provide new insight into a transcriptional regulator and a transport system that play essential roles in the ability of P. syringae pv. tomato DC3000 to resist the action of flavonoids produced by the host.

scholarly journals A revised model for the role of GacS/GacA in regulating type III secretion by Pseudomonas syringae pv. tomato DC3000

2019 ◽  
Vol 21 (1) ◽  
pp. 139-144 ◽  
Author(s):  
Megan R. O’Malley ◽  
Ching‐Fang Chien ◽  
Scott C. Peck ◽  
Nai‐Chun Lin ◽  
Jeffrey C. Anderson
Keyword(s):  
Pseudomonas Syringae ◽  
Type Iii Secretion ◽  
Tomato Dc3000 ◽  
Type Iii

scholarly journals Identification and Characterization of a Well-Defined Series of Coronatine Biosynthetic Mutants of Pseudomonas syringae pv. tomato DC3000

2004 ◽  
Vol 17 (2) ◽  
pp. 162-174 ◽  
Author(s):  
David M. Brooks ◽  
Gustavo Hernández-Guzmán ◽  
Andrew P. Kloek ◽  
Francisco Alarcón-Chaidez ◽  
Aswathy Sreedharan ◽  
...  
Keyword(s):  
Pseudomonas Syringae ◽  
High Performance ◽  
Tomato Dc3000 ◽  
Disease Symptoms ◽  
Coronafacic Acid ◽  
Tn5 Mutants ◽  
Cor Genes ◽  
Identification And Characterization

To identify Pseudomonas syringae pv. tomato genes involved in pathogenesis, we carried out a screen for Tn5 mutants of P. syringae pv. tomato DC3000 with reduced virulence on Arabidopsis thaliana. Several mutants defining both known and novel virulence loci were identified. Six mutants contained insertions in biosynthetic genes for the phytotoxin coronatine (COR). The P. syringae pv. tomato DC3000 COR genes are chromosomally encoded and are arranged in two separate clusters, which encode enzymes responsible for the synthesis of coronafacic acid (CFA) or coronamic acid (CMA), the two defined intermediates in COR biosynthesis. High-performance liquid chromatography fractionation and exogenous feeding studies confirmed that Tn5 insertions in the cfa and cma genes disrupt CFA and CMA biosynthesis, respectively. All six COR biosynthetic mutants were significantly impaired in their ability to multiply to high levels and to elicit disease symptoms on A. thaliana plants. To assess the relative contributions of CFA, CMA, and COR in virulence, we constructed and characterized cfa6 cmaA double mutant strains. These exhibited virulence phenotypes on A. thalliana identical to those observed for the cmaA or cfa6 single mutants, suggesting that reduced virulence of these mutants on A. thaliana is caused by the absence of the intact COR toxin. This is the first study to use biochemically and genetically defined COR mutants to address the role of COR in pathogenesis.

scholarly journals Role of Jasmonic Acid Pathway in Tomato Plant-Pseudomonas syringae Interaction

Plants ◽  
2020 ◽  
Vol 9 (2) ◽  
pp. 136 ◽  
Author(s):  
Loredana Scalschi ◽  
Eugenio Llorens ◽  
Pilar García-Agustín ◽  
Begonya Vicedo
Keyword(s):  
Gene Expression ◽  
Salicylic Acid ◽  
Jasmonic Acid ◽  
Pseudomonas Syringae ◽  
Tomato Plant ◽  
Wild Type ◽  
Tomato Plants ◽  
Bacterial Pathogenicity ◽  
Acid Pathway

The jasmonic acid pathway has been considered as the backbone of the response against necrotrophic pathogens. However, a hemi-biotrophic pathogen, such as Pseudomonas syringae, has taken advantage of the crosstalk between the different plant hormones in order to manipulate the responses for its own interest. Despite that, the way in which Pseudomonas syringae releases coronatine to activate jasmonic acid-derived responses and block the activation of salicylic acid-mediated responses is widely known. However, the implication of the jasmonic intermediates in the plant-Pseudomonas interaction is not studied yet. In this work, we analyzed the response of both, plant and bacteria using SiOPR3 tomato plants. Interestingly, SiOPR3 plants are more resistant to infection with Pseudomonas. The gene expression of bacteria showed that, in SiOPR3 plants, the activation of pathogenicity is repressed in comparison to wild type plants, suggesting that the jasmonic acid pathway might play a role in the pathogenicity of the bacteria. Moreover, treatments with JA restore the susceptibility as well as activate the expression of bacterial pathogenicity genes. The observed results suggest that a complete jasmonic acid pathway is necessary for the susceptibility of tomato plants to Pseudomonas syringae.

scholarly journals Contribution of the non-effector members of the HrpL regulon, iaaL and matE, to the virulence of Pseudomonas syringae pv. tomato DC3000 in tomato plants

2015 ◽  
Vol 15 (1) ◽  
Author(s):  
Melissa G. Castillo-Lizardo ◽  
Isabel M. Aragón ◽  
Vivian Carvajal ◽  
Isabel M. Matas ◽  
María Luisa Pérez-Bueno ◽  
...  
Keyword(s):  
Pseudomonas Syringae ◽  
Tomato Dc3000 ◽  
Tomato Plants

Bi2O3/TiO2@reduced graphene oxide with enzyme-like properties efficiently inactivates Pseudomonas syringae pv. tomato DC3000 and enhances abiotic stress tolerance in tomato

2021 ◽  
Author(s):  
Hui Yu ◽  
Lei Wang ◽  
Jianhua Qu ◽  
Xu Wang ◽  
Fuxin Huang ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Graphene Oxide ◽  
Reduced Graphene Oxide ◽  
Pseudomonas Syringae ◽  
Pathogenic Bacteria ◽  
Potential Role ◽  
Abiotic Stress Tolerance ◽  
Tomato Dc3000 ◽  
Reduced Graphene

This study aimed to evaluate the potential role of Bi2O3/TiO2@reduced graphene oxide (rGO) on the antibacterial activity of the typical plant pathogenic bacteria Pst.DC3000 and the enhancement of tomato resistance...

scholarly journals CorR Regulates Multiple Components of Virulence in Pseudomonas syringae pv. tomato DC3000

2006 ◽  
Vol 19 (7) ◽  
pp. 768-779 ◽  
Author(s):  
Aswathy Sreedharan ◽  
Alejandro Penaloza-Vazquez ◽  
Barbara N. Kunkel ◽  
Carol L. Bender
Keyword(s):  
Pseudomonas Syringae ◽  
Biosynthetic Pathway ◽  
Response Regulator ◽  
Tomato Dc3000 ◽  
Tomato Plants ◽  
Coronafacic Acid ◽  
Multiple Components ◽  
Expression Of Genes ◽  
Genes Encoding ◽  
Polymerase Chain

The phytotoxin coronatine (COR) is produced by various pathovars of Pseudomonas syringae, including P. syringae pv. tomato DC3000, which is pathogenic on crucifers and tomato, and P. syringae pv. glycinea PG4180, a soybean pathogen. The COR molecule contains two distinct components, coronafacic acid (CFA) and coronamic acid (CMA), which are intermediates in the COR biosynthetic pathway. In P. syringae pv. tomato DC3000, it is not clear whether corR, which encodes a response regulator, positively regulates CFA and CMA synthesis as it does in P. syringae pv. glycinea PG4180. In this study, a corR mutant of P. syringae pv. tomato DC3000 was constructed and was shown to be defective in the production of COR, CFA, and CMA. Furthermore, disease severity was greatly reduced in tomato plants inoculated with the corR mutant compared with wild-type P. syringae pv. tomato DC3000. We also showed that a mutation in hrpL, which encodes an alternate RNA polymerase sigma factor (σL) required for the expression of genes encoding components of the type III secretion system, abrogated production of COR in P. syringae pv. tomato DC3000. The presence of a potential hrp box, the recognition site for σL, upstream of corR suggested that corR might be regulated by hrpL. This was confirmed in reverse-transcription polymerase chain reaction experiments showing that the upstream effector gene holPtoAA, which was associated with the hrp box, was cotranscribed with corR. Furthermore, studies also were conducted to investigate whether mutations in corR had effects on the expression of hrpL. The corR mutant of P. syringae pv. tomato DC3000 showed both a reduction and delay in the expression of hrpL and was impaired in its ability to elicit a hypersensitive response on Nicotiana benthamiana. A putative CorR-binding site was identified upstream of hrpL, and gel shift studies confirmed the binding of CorR to this region. These results indicate that corR directly impacts the expression of the hrp regulon in P. syringae.

scholarly journals A Pseudomonas syringae pv. tomato DC3000 Hrp (Type III Secretion) Deletion Mutant Expressing the Hrp System of Bean Pathogen P. syringae pv. syringae 61 Retains Normal Host Specificity for Tomato

2003 ◽  
Vol 16 (1) ◽  
pp. 43-52 ◽  
Author(s):  
Derrick E. Fouts ◽  
Jorge L. Badel ◽  
Adela R. Ramos ◽  
Ryan A. Rapp ◽  
Alan Collmer
Keyword(s):  
Host Range ◽  
Resistance Gene ◽  
Host Specificity ◽  
Pseudomonas Syringae ◽  
Type Iii Secretion ◽  
Secretion System ◽  
Effector Proteins ◽  
Tomato Dc3000 ◽  
Tomato Plants ◽  
Type Iii

The plant pathogenic species Pseudomonas syringae is divided into numerous pathovars based on host specificity. For example, P. syringae pv. tomato DC3000 is pathogenic on tomato and Arabidopsis, whereas P. syringae pv. syringae 61 is pathogenic on bean. The ability of P. syringae strains to elicit the hypersensitive response (HR) in non-hosts or be pathogenic (or parasitic) in hosts is dependent on the Hrp (type III secretion) system and effector proteins this system is thought to inject into plant cells. To test the role of the Hrp system in determining host range, the hrp/hrc gene cluster (hrpK through hrpR) was deleted from DC3000 and complemented in trans with the orthologous cluster from strain 61. Mutant CUCPB5114 expressing the bean pathogen Hrp system on plasmid pCPP2071 retained the ability of wild-type DC3000 to elicit the HR in bean, to grow and cause bacterial speck in tomato, and to elicit a cultivar-specific (gene-for-gene) HR in tomato plants carrying the Pto resistance gene. However, the symptoms produced in compatible tomato plants involved markedly reduced chlorosis, and CUCPB5114(pCPP2071) did not grow or produce symptoms in Arabidopsis Col-0 although it was weakly virulent in NahG Arabidopsis. A hypersensitive-like collapse was produced by CUCPB5114(pCPP2071) in Arabidopsis Col-0 at 1 × 107 CFU/ml, but only if the bacteria also expressed AvrB, which is recognized by the RPM1 resistance gene in Col-0 and confers incompatibility. These observations support the concept that the P. syringae effector proteins, rather than secretion system components, are the primary determinants of host range at both the species and cultivar levels of host specificity.

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