scholarly journals Complete genomic sequences of Propionibacterium freudenreichii phages from Swiss cheese reveal greater diversity than Cutibacterium (formerly Propionibacterium) acnes phages

2018 ◽  
Vol 18 (1) ◽  
Author(s):  
Lucy Cheng ◽  
Laura J. Marinelli ◽  
Noël Grosset ◽  
Sorel T. Fitz-Gibbon ◽  
Charles A. Bowman ◽  
...  
Keyword(s):  
Propionibacterium Acnes ◽  
Genomic Sequences ◽  
Propionibacterium Freudenreichii ◽  
Swiss Cheese ◽  
Complete Genomic

First Detection and Genetic Characterization of Equus Caballus Papillomavirus 1, 2 and 7 in China

2021 ◽  
Author(s):  
Panpan Tong ◽  
Xiaozhen Song ◽  
Meiling Ren ◽  
Erken Jia ◽  
Lei Zhang ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Epidemiological Study ◽  
Genetic Characterization ◽  
Equus Caballus ◽  
Genomic Sequences ◽  
Sequence Analyses ◽  
Pioneer Study ◽  
Nasal Swabs ◽  
Complete Genomic

Abstract Background: Nine species of Equus caballus papillomavirus (EcPV) have been reported to infect horses, however, there are so far no reports of such infections in China. Results: In our pioneer study with Chinese horses, we found EcPV-1 in intranasal papilloma and nasal swabs, EcPV-2 in nasal swabs and semen, and EcPV-7 primarily in semen. This indicates that EcPVs are indeed hosted by horses in China, and that EcPV-2 and 7 may be getting transmitted though breeding. Sequence analyses for complete genomic sequences of EcPV-1 (G2), EcPV-2 (XJ-KS1391) and EcPV-7 (XJ-zs1) were performed which indicated that EcPV-1, 2 and 7, that infect horses in China, share 99.3% nt identity with the already published sequences for EcPV-1, 2 and 7. These observations indicate that three types of EcPVs identified in the current study are highly similar variants of previously known types of EcPV-1, 2 and 7. Phylogenetic analysis based on L1 genes in GenBank showed that EcPV-1, 2 and 7, found in Chinese horses, are closely related to and clustered together with already known EcPV-1, 2 and 7, respectively. Conclusion: Our study provides a novel evidence for EcPVs infection and circulation in Chinese horses and thus lays the foundation for a systematic and detailed epidemiological study of these infections in Chinese horses.

scholarly journals Complete Genomic Sequences of H3N8 Equine Influenza Virus Strains Used as Vaccine Strains in Japan

2018 ◽  
Vol 6 (12) ◽  
Author(s):  
Manabu Nemoto ◽  
Takashi Yamanaka ◽  
Hiroshi Bannai ◽  
Koji Tsujimura ◽  
Hiroshi Kokado
Keyword(s):  
Influenza Virus ◽  
Influenza A Virus ◽  
Influenza A ◽  
Animal Health ◽  
Genomic Sequences ◽  
Equine Influenza Virus ◽  
Equine Influenza ◽  
World Organization ◽  
Virus Strains ◽  
Complete Genomic

ABSTRACT We sequenced the eight segments of influenza A virus strains A/equine/Ibaraki/1/2007 and A/equine/Yokohama/aq13/2010, which are strains of the Florida sublineage clades 1 and 2 of the H3N8 subtype equine influenza virus. These strains have been used as vaccine strains in Japan since 2016 in accordance with World Organization for Animal Health (OIE) recommendations.

scholarly journals Isovaleric acid is mainly produced by Propionibacterium freudenreichii in Swiss cheese

2004 ◽  
Vol 14 (9) ◽  
pp. 801-807 ◽  
Author(s):  
Anne Thierry ◽  
Romain Richoux ◽  
Jean-René Kerjean
Keyword(s):  
Isovaleric Acid ◽  
Propionibacterium Freudenreichii ◽  
Swiss Cheese

Characterization of intronic structures and alternative splicing in Phytophthora sojae by comparative analysis of expressed sequence tags and genomic sequences

2011 ◽  
Vol 57 (2) ◽  
pp. 84-90 ◽  
Author(s):  
Danyu Shen ◽  
Wenwu Ye ◽  
Suomeng Dong ◽  
Yuanchao Wang ◽  
Daolong Dou
Keyword(s):  
Comparative Analysis ◽  
Alternative Splicing ◽  
Expressed Sequence Tags ◽  
Phytophthora Sojae ◽  
Genomic Sequences ◽  
Splice Sites ◽  
Distinct Phylogenetic Lineage ◽  
Expressed Sequence ◽  
Complete Genomic

The oomycetes, a distinct phylogenetic lineage of fungus-like microorganisms, are heterokonts (stramenopiles) belonging to the supergroup Chromalveolata. Although the complete genomic sequences of a number of oomycetes have been reported, little information regarding the introns therein is available. Here, we investigated the introns of Phytophthora sojae , a pathogen that causes soybean root and stem rot, by a comparative analysis of genomic sequences and expressed sequence tags. A total of 4013 introns were identified, of which 96.6% contained canonical splice sites. The P. sojae genome possessed features distinct from other organisms at 5′ splice sites, polypyrimidine tracts, branch sites, and 3′ splice sites. Diverse repeating sequences, ranging from 2 to 10 nucleotides in length, were found at more than half of the intron–exon boundaries. Furthermore, 122 genes underwent alternative splicing. These data indicate that P. sojae has unique splicing mechanisms, and recognition of those mechanisms may lead to more accurate predictions of the location of introns in P. sojae and even other oomycete species.

Genetic characterization of Chinese measles vaccines by analysis of complete genomic sequences

2009 ◽  
Vol 81 (8) ◽  
pp. 1477-1483 ◽  
Author(s):  
Yan Zhang ◽  
Jianhui Zhou ◽  
William J. Bellini ◽  
Wenbo Xu ◽  
Paul A. Rota
Keyword(s):  
Genetic Characterization ◽  
Genomic Sequences ◽  
Complete Genomic

scholarly journals Autolysis of Lactobacillus helveticus and Propionibacterium freudenreichii in Swiss cheeses: first evidence by using species-specific lysis markers

1998 ◽  
Vol 65 (4) ◽  
pp. 609-620 ◽  
Author(s):  
FLORENCE VALENCE ◽  
ROMAIN RICHOUX ◽  
ANNE THIERRY ◽  
AIRI PALVA ◽  
SYLVIE LORTAL
Keyword(s):  
Coenzyme A ◽  
Streptococcus Thermophilus ◽  
Lactobacillus Helveticus ◽  
Propionibacterium Freudenreichii ◽  
Swiss Cheese ◽  
Specific Lysis ◽  
Cold Room ◽  
Sds Page ◽  
Intracellular Proteins ◽  
Species Specific

Lactobacillus helveticus and Propionibacterium freudenreichii are essential starters in Swiss cheesemaking and the release of their intracellular enzymes through autolysis could significantly influence ripening. To provide evidence of this lysis, cheese made from microfiltered thermized milk inoculated with Lb. helveticus ITGLH77, Prop. freudenreichii ITGP23 and a commercial Streptococcus thermophilus was assayed. Starter viability was determined and lysis was monitored during ripening by protein analysis with SDS-PAGE of aqueous cheese extracts and by immunoblot detection of intracellular proteins: dipeptidase (PepD) for Lb. helveticus and methylmalonyl coenzyme A mutase for Prop. freudenreichii. We verified that the species specificity of these lysis markers was towards the cytoplasms of all the species currently used in Swiss cheese. Lb. helveticus exhibited an almost complete loss of viability (99·9%) from the beginning of ripening in the cold room; concomitantly PepD appeared in the cheese extracts and was detected until the end of ripening. Damaged Lb. helveticus cells were also visualized by scanning electron microscopy. In addition, free PepD was also successfully detected in commercial Swiss-related cheeses. All these results clearly demonstrated the autolysis of Lb. helveticus in Swiss cheese. Prop. freudenreichii ITGP23 grew during warm room ripening and no loss of viability was detected after maximal growth (109 cfu/g cheese). Free methylmalonyl-coenzyme A mutase was detected at the end of ripening during cold storage, when the cheese extracts were concentrated 20-fold, demonstrating that the autolysis of Prop. freudenreichii was tardy and limited.

scholarly journals Origin, Evolution, and Genotyping of Emergent Porcine Epidemic Diarrhea Virus Strains in the United States

mBio ◽  
2013 ◽  
Vol 4 (5) ◽  
Author(s):  
Yao-Wei Huang ◽  
Allan W. Dickerman ◽  
Pablo Piñeyro ◽  
Long Li ◽  
Li Fang ◽  
...  
Keyword(s):  
United States ◽  
Porcine Epidemic Diarrhea Virus ◽  
The United States ◽  
Genomic Sequences ◽  
Evolutionary Divergence ◽  
Origin And Evolution ◽  
Diarrhea Virus ◽  
Porcine Epidemic Diarrhea ◽  
And Control ◽  
Complete Genomic

ABSTRACT Coronaviruses are known to infect humans and other animals and cause respiratory and gastrointestinal diseases. Here we report the emergence of porcine epidemic diarrhea virus (PEDV) in the United States and determination of its origin, evolution, and genotypes based on temporal and geographical evidence. Histological lesions in small intestine sections of affected pigs and the complete genomic sequences of three emergent strains of PEDV isolated from outbreaks in Minnesota and Iowa were characterized. Genetic and phylogenetic analyses of the three U.S. strains revealed a close relationship with Chinese PEDV strains and their likely Chinese origin. The U.S. PEDV strains underwent evolutionary divergence, which can be classified into two sublineages. The three emergent U.S. strains are most closely related to a strain isolated in 2012 from Anhui Province in China, which might be the result of multiple recombination events between different genetic lineages or sublineages of PEDV. Molecular clock analysis of the divergent time based on the complete genomic sequences is consistent with the actual time difference, approximately 2 to 3 years, of the PED outbreaks between China (December 2010) and the United States (May 2013). The finding that the emergent U.S. PEDV strains share unique genetic features at the 5′-untranslated region with a bat coronavirus provided further support of the evolutionary origin of PEDV from bats and potential cross-species transmission. The data from this study have important implications for understanding the ongoing PEDV outbreaks in the United States and will guide future efforts to develop effective preventive and control measures against PEDV. IMPORTANCE The sudden emergence of porcine epidemic diarrhea virus (PEDV), a coronavirus, for the first time in the United States causes significant economic and public health concerns. Since its recognition in May 2013, PEDV has rapidly spread across the United States, resulting in high mortality in piglets in more than 17 States now. The ongoing outbreaks of Middle East respiratory syndrome coronavirus in humans from countries in or near the Arabian Peninsula and the historical deadly nature of the 2002 outbreaks of severe acute respiratory syndrome coronavirus create further anxiety over the emergence of PEDV in the United States due to the lack of scientific information about the origin and evolution of this emerging coronavirus. Here we report the detailed genetic characterization, origin, and evolution of emergent PEDV strains in the United States. The results provide much needed information to devise effective preventive and control strategies against PEDV in the United States.

scholarly journals Propionibacterium freudenreichii strains quantitatively affect production of volatile compounds in Swiss cheese

2005 ◽  
Vol 85 (1-2) ◽  
pp. 57-74 ◽  
Author(s):  
Anne Thierry ◽  
Marie-Bernadette Maillard ◽  
Romain Richoux ◽  
Jean-Ren� Kerjean ◽  
Sylvie Lortal
Keyword(s):  
Volatile Compounds ◽  
Propionibacterium Freudenreichii ◽  
Swiss Cheese

scholarly journals Complete Genomic Sequences of Three Salmonella enterica subsp. enterica Serovar Muenchen Strains from an Orchard in San Joaquin County, California

2020 ◽  
Vol 9 (13) ◽  
Author(s):  
Thao D. Tran ◽  
Steven Huynh ◽  
Craig T. Parker ◽  
Robert Hnasko ◽  
Lisa Gorski ◽  
...  
Keyword(s):  
Salmonella Enterica ◽  
Complete Genome ◽  
Genomic Sequences ◽  
Genome Sequences ◽  
Content Type ◽  
San Joaquin County ◽  
Complete Genomic

We present here the complete genome sequences of three Salmonella enterica subsp. enterica serovar Muenchen strains, LG24, LG25, and LG26. All three strains were isolated from almond drupes grown in an orchard in San Joaquin County, California, in 2016. These genomic sequences are nonidentical and will contribute to our understanding of S. enterica genomics.

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