scholarly journals Identification, gene expression and genetic polymorphism of zinc finger A20/AN1 stress-associated genes, HvSAP, in salt stressed barley from Kazakhstan

2020 ◽  
Vol 20 (S1) ◽  
Author(s):  
Akmaral Baidyussen ◽  
Maryam Aldammas ◽  
Akhylbek Kurishbayev ◽  
Malika Myrzabaeva ◽  
Askar Zhubatkanov ◽  
...  

Abstract Background A family of genes designated as the Zinc finger A20/AN1 Transcription factors encoding stress-associated proteins (SAP) are well described in Arabidopsis and rice, and include 14 AtSAP and 18 OsSAP genes that are associated with variable tolerances to multiple abiotic stresses. The SAP gene family displays a great diversity in its structure and across different plant species. The aim of this study was to identify all HvSAP genes in barley (Hordeum vulgare L.), to analyse the expression of selected genes in response to salinity in barley leaves and develop SNP marker for HvSAP12 to evaluate the association between genotypes of barley plants and their grain yield in field trials. Results In our study, 17 HvSAP genes were identified in barley, which were strongly homologous to rice genes. Five genes, HvSAP5, HvSAP6, HvSAP11, HvSAP12 and HvSAP15, were found to be highly expressed in leaves of barley plants in response to salt stress in hydroponics compared to controls, using both semi-quantitative RT-PCR and qPCR analyses. The Amplifluor-like SNP marker KATU-B30 was developed and used for HvSAP12 genotyping. A strong association (R2 = 0.85) was found between KATU-B30 and grain yield production per plant of 50 F3 breeding lines originating from the cross Granal × Baisheshek in field trials with drought and low to moderate salinity in Northern and Central Kazakhstan. Conclusions A group of HvSAP genes, and HvSAP12 in particular, play an important role in the tolerance of barley plants to salinity and drought, and is associated with higher grain yield in field trials. Marker-assisted selection with SNP marker KATU-B30 can be applied in barley breeding to improve grain yield production under conditions of abiotic stress.

1993 ◽  
Vol 121 (1) ◽  
pp. 13-19 ◽  
Author(s):  
P. Peltonen-Sainio ◽  
K. Moore ◽  
E. Pehu

SUMMARYThe phenotypic stability of seven Finnish and Nordic oat cultivars and 12 breeding lines developed at the Hankkija Plant Breeding Institute, Finland, was studied using the Finlay–Wilkinson regression method, Eberhart–Russell stability analysis, principal component analysis (PCA) with biplot presentation, canonical variate analysis (CVA) and Procrustes analysis. Comparison of stability analyses was based on four dissimilar growing seasons. In addition to the measurement of phenotypic stability of grain yield, the performance of 12 morpho-physiological traits was evaluated, including maturity class and structure of canopy and plant stand.The different stability analyses examined produced uniform results and ranked the cultivars and breeding lines without major disagreements. Lines with exceptionally stable yield performance over the different growing seasons were identified. In general, there was an association of high yielding ability with poor stability. However, some cultivars deviated from this tendency and showed high grain yield production and average stability of yield performance.


2018 ◽  
Vol 17 (03) ◽  
pp. 289-292
Author(s):  
Pranesh ◽  
S. Ramesh

AbstractProtein energy malnutrition (PEM) is prevalent in south-east Asian countries including India. Breeding and introduction of grain protein-rich varieties of legumes such as dolichos bean is considered as cost-effective approach to combat PEM. Exploitation of genetic variability within germplasm accessions (GAs) and/or breeding populations is the short-term strategy for identification and delivery of protein-rich dolichos bean cultivars to cater to the immediate needs of the farmers and target population. A set of 118 dolichos bean genotypes consisting of 96 GAs and 20 advanced breeding lines (ABLs) and two released varieties (RVs) was field evaluated in augmented deign for dry grain yield per plant and their grain protein contents were estimated. The grain protein content among the genotypes ranged from 18.82 to 24.5% with a mean of 21.73%. The magnitude of estimates of absolute range, standardized range, and phenotypic coefficient of variation (PCV) for grain protein content was higher among GAs than those among ABLs + RVs. However, average grain protein contents of GAs were comparable to those of ABLs + RVs. Nearly 50% of the genotypes (mostly GAs) had significantly higher grain protein content than those of RVs, HA 3 and HA 4. The grain protein contents of the genotypes were poorly correlated with grain yield per plant. These results are discussed in relation to strategies to breed grain protein-rich dolichos bean cultivars.


2013 ◽  
Vol 13 (4) ◽  
pp. 234-240 ◽  
Author(s):  
Giovani Benin ◽  
Lindolfo Storck ◽  
Volmir Sérgio Marchioro ◽  
Francisco de Assis Franco ◽  
Ivan Schuster ◽  
...  

The aim of this study was to verify whether using the Papadakis method improves model assumptions and experimental accuracy in field trials used to determine grain yield for wheat lineages indifferent Value for Cultivation and Use (VCU) regions. Grain yield data from 572 field trials at 31 locations in the VCU Regions 1, 2, 3 and 4 in 2007-2011 were used. Each trial was run with and without the use of the Papadakis method. The Papadakis method improved the indices of experimental precision measures and reduced the number of experimental repetitions required to predict grain yield performance among the wheat genotypes. There were differences among the wheat adaptation regions in terms of the efficiency of the Papadakis method, the adjustment coefficient of the genotype averages and the increases in the selective accuracy of grain yield.


2017 ◽  
Vol 47 (3) ◽  
pp. 185-194 ◽  
Author(s):  
Adriano Stephan NASCENTE ◽  
Ruby KROMOCARDI

ABSTRACT The upland rice farmers in Suriname use local varieties and low level technologies in the field. As a result, the upland rice grain yield is low, at about 1 000 kg ha-1. Our objective was to evaluate the use of upland rice cultivars from Suriname and Brazil, and the effect of nitrogen, N, phosphorus, P, and potassium, K, fertilizers on cultivation variables. We undertook four field trials in the Victoria Area, in the Brokopondo District, using a randomized block design each with four replications. The most productive rice varieties were BRS Esmeralda (grain yield 2 903 kg ha-1) and BRS Sertaneja (2 802 kg ha-1). The highest grain yield of 2 620 kg ha-1 was achieved with a top dressing application of 76.41 kg N ha-1 20 days after sowing. For P, the highest grain yield of 3 085 kg ha-1 was achieved with application of 98.06 kg ha-1 P2O5 applied at sowing. An application rate of 31.45 kg ha-1 of K2O at sowing achieved the highest grain yield of 2 952 kg ha-1. Together, these application rates of N, P and K resulted in rice grain yield of about 3 000 kg ha-1, which is three times greater than the national average for upland rice. We demonstrate that the use of improved rice varieties matched to the local conditions, and application of appropriate fertilizers, are management practices that can result in significant increases in rice grain yield in Suriname.


1998 ◽  
Vol 130 (3) ◽  
pp. 287-295 ◽  
Author(s):  
C. L. MORGAN

Twenty-eight F1 hybrids of wheat and their parents were grown in field trials at Trumpington, Cambridge during 1986/87 and 1987/88. They were derived from crosses between seven ‘modern’ varieties, used as female parents, and either two ‘old’ (Squareheads Master and Partridge) or two ‘modern’ varieties (Bert and Motto), which were used as male parents. Grain yield, yield components, biomass and height were determined. The male parents were chosen to provide contrasting phenotypes and genetic backgrounds for the F1 hybrids. Mid-parent advantage, the increase of a hybrid for a given character above the mean of its parents, and heterosis, the increase of a hybrid above the ‘better’ parent for that character, were calculated. Most F1 hybrids showed mid-parent advantage for the characters studied. This tended to be greatest for hybrids derived from parents with the largest phenotypic differences in that character. In contrast, where heterosis occurred it tended to be greatest where the phenotypic difference between the parents was least. This suggests that the beneficial effects of hybridization, resulting from the dispersion of dominant genes between the parents, was insufficient to overcome the detrimental effects of other genes present where the ‘less good’ parent was substantially lower than the ‘better’ parent. Hybrids derived from the ‘modern’ male parents had greater heterosis for grain yield and mean grain weight than those from the ‘old’ parents. Of the yield components, positive heterosis for mean grain weight resulted in heavier seeds and was the most important yield component in determining heterosis in grain yield. Heterosis for the number of grains/ear was small or did not differ significantly from zero while number of ears/m2 showed negative heterosis resulting in fewer ears/m2 in the hybrids.


2016 ◽  
Vol 10 (1) ◽  
pp. 7-9
Author(s):  
Jiban Shrestha

The present study pertaining to the identification of high yielding quality protein maize genotypes was conducted under coordinated varietal trials (CVT) at Khumaltar (Lalitpur) and Kabre (Dolakha) from 2011 to 2012 summer season and under coordinated farmers' field trials (CFFT) at Lumle (Kaski), Kimugaun (Dailekh) and Kabre (Dolakha) in 2011 summer season and at Dailekh and Kabre in 2012 summer season. The results of these trials showed that the genotypes namely Obatanpa followed by S99TLWQ-B and S01SIYQ produced the highest grain yield under coordinated varietal trials and genotypes namely S99TLYQ-B followed by S01SIWQ-3 and Poshilo Makai-1 produced the highest grain yield under coordinated farmers' field trials across testing sites and years. It is suggested that the superior genotypes derived from CFFT could be released and then recommended to farmers of mid hills of Nepal.International Journal of Life Sciences 10 (1) : 2016; 7-9


2005 ◽  
Vol 130 (2) ◽  
pp. 196-202 ◽  
Author(s):  
Marilyn H.Y. Hovius ◽  
Irwin L. Goldman ◽  
Kirk L. Parkin

Breeders have found field screening for white rot (Sclerotium cepivorum Berk.) resistance in onion (Allium cepa L.) to be unreliable since consistently moderate to high disease levels that significantly differentiate cultivars do not occur over field sites and years. The objective was to determine if differences in onion white rot resistance levels were associated with differing S-alk(en)yl-l-cysteine sulfoxide (ACSO) levels. A collection of onion breeding lines and hybrids were evaluated in field trials at six sites in 1999-2001. High performance liquid chromatography was used to analyze ACSOs in onion plant organs. Four main cysteine-sulfoxides exist in Allium L. species: methyl (MCSO), 2-propenyl (2-PeCSO), 1-propenyl (1-PeCSO), and propyl (PCSO). 1-PeCSO was predominant in onion leaves, bulbs, and roots. 2-PeCSO was found in trace amounts in onion leaves and roots. There was significantly more 2-PeCSO and total ACSO (roots only) and 1-PeCSO (roots and bulbs) in accessions that were more susceptible to white rot in the field trials. This is the first report of significant differences in ACSO contents among white rot susceptible and resistant onions. A covariance analysis was used to determine if the ACSO levels that significantly distinguished among accessions could predict field onion white rot reaction. 1-PeCSO from both roots and bulbs was the best predictor of field disease incidence in field sites that had low, moderate, and high disease levels. Although the ACSO concentrations were not assessed on an individual plant basis, breeders may be able to screen onions for resistance to S. cepivorum by comparing onion root or bulb 1-PeCSO levels based on the results from this research. White rot incidence in the field should be higher in those plants whose roots and bulbs have the highest levels of 1-PeCSO.


1993 ◽  
Vol 118 (4) ◽  
pp. 525-530 ◽  
Author(s):  
Sanford D. Eigenbrode ◽  
John T. Trumble ◽  
Richard A. Jones

Accessions of Lycopersicon esculentum var. cerasiforme (Dun.) A. Gray (cer) and L. pimpinellifolium (Jusl.) Mill. (pimp), sustained significantly less damage to fruit by beet armyworm [Spodoptera exigua (Hiibner)] than standard cultivars and breeding lines of L. esculentum Mill. (esc) under natural infestations in 1990 and 1991 in southern California. The dwarf vine cherry cultivar Tiny Tim also sustained less damage than the standards. Accessions of esc with various monogenic mutations sustained at least as much beet armyworm damage as did standard cultivars. The percentage of fruit damaged was significantly correlated with vine weight, weight per fruit, number of fruit, and the fruit-foliage weight ratio (Pearson's coefficients, respectively: -0.533, 0.450, -0.483, 0.390, n = 37). In laboratory assays, survival of beet armyworm was significantly lower (5% of susceptible& growth rates were significantly lower, and development time was significantly longer on the fruit of resistant `Tiny Tim' and LA 1320 cer than the fruit of 11 other test lines. There were no substantial differences in beet armyworm survival on the foliage of the test lines. In the field trials, there were also significant differences among the test lines in damage by Liriomyza species and hemipteran pests. Lines with genes for increased densities of nonglandular leaf trichomes (especially LA 1663) were generally less damaged by Liriomyza than other lines. Damage by hemipterans was correlated with vine and fruit size, fruit count, and fruit-foliage weight ratio in 1991, but high intraseason variability prevented clear identification of test lines resistant to these pests.


Author(s):  
P. А. Ageeva ◽  
М. V. Matyukhina ◽  
N. А. Pochutina ◽  
O. M. Gromova

The narrow-leafed lupin (Lupinus angustifolius L.) is a valuable legumes crop used as forage and green manure which is adapted to wide spectrum of soil-and climatic conditions; the crop has short domestication history. The protein content in its seeds and in dry matter of green mass varies from 30.0 to 37.0% and from 16.0 to 22.0 % respectively and depends on ecotype and soil-and-climatic conditions. This lupin specie can accumulate to 300 kg/ha symbiotic nitrogen in biomass and assimilates phosphorus and potassium of heavy available soil layers. It is very technological suitable for common used machinery systems. The State List of breeding achievements of Russia recommends the following regions for lupin cultivation: the North, the North-West, the Central, the Volga-Vyatka, the Middle-Volga, the Central Chernozem, the Ural, the West Siberia and the East Siberia. The tests were carried out in 2017-2020 in the All-Russian Lupin Scientific Research Institute which is located in the South-West of the Central region. Ten varieties and breeding lines bred in the Institute are tested. The samples differ by early ripeness and anthracnose tolerance. The average experimental variety grain yield was 2.38 t/ha. The vars. Uzkolistny 53-02, USN 53-236, Bryanskiy kormovoy and SBS 56-15 have the highest yield and adaptivity (103-113 %). The index of year conditions was revealed; 2017 with the index 0.56 was the most favorable for implementation of grain productivity of the tested narrow-leafed lupin varieties. In the ecological varieties testing the soil-and-climatic conditions of Shatilovskaya experimental station (Orel region) were the most favorable for implementation of variety grain productivity (4.0-4.5 t/ha). Grain yield was 3.0-4.0 t/ha in ecological locations which differ in soil-and-climatic conditions: there are Kaliningrad region, Mordovia Republic, Krasnoyarsk region etc.


2020 ◽  
Vol 1 (1) ◽  
pp. 45-49
Author(s):  
Tsotne Samadashvili ◽  
Gulnari Chkhutiashvili ◽  
Mirian Chokheli ◽  
Zoia Sikharulidze ◽  
Qetevan Nacarishvili

Wheat is a vital crop in Georgia and in the world. Because of the increase in the rate of population growth, improving the grain yield is the way to meet food demand. Proper crop nutrition plays a vital role in maintaining the world’s food supply. Fertilizer is essential for accomplishing this.One of the most important means for increasing the wheat yield is fertilizer, especially, organic fertilizer. The present research was carried out to study the effects of different doses (150ml, 200ml and 300 ml on ha) of humic organic fertilizer “Ecorost” on yield of winter wheat cultivar “Tbilisuri 15”. The humic liquid fertilizer "Ecorost" is a peat-based organic-mineral fertilizer. The product is active and saturated due to the use of the latest technology and living bacteria found in peat. The field trials were conducted in 2017-2019 at the Experimental Site of Scientific Research Center of Agriculture in Dedopliskharo- arid region (Eastern Georgia).Liquid fertilizer was applied two times: in tillering stage in early spring and two weeks after - in stem elongation stage. Results indicated that the highest wheat grain yield (4t/ha) was achieved when the plants were fertilized with 300 ml on 1 ha ofEcorost. Applications of liquid fertilizer “Ecorost” increased grain yield of winter wheat by 16.2% in comparison with standard nitrogen fertilization. Thus, liquid fertilizer “Ecorost” had a significant effect on wheat grain yield compared to control standard nitrogen fertilizer.


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