scholarly journals Functional gene assessment of bread wheat: breeding implications in Ningxia Province

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Weijun Zhang ◽  
Junjie Zhao ◽  
Jinshang He ◽  
Ling Kang ◽  
Xiaoliang Wang ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Genetic Resources ◽  
Bread Wheat ◽  
Large Population ◽  
Flow Analysis ◽  
Wheat Breeding ◽  
Wheat Varieties ◽  
Genetic Components ◽  
Yield Quality ◽  
Genetic Distribution

Abstract Background The overall genetic distribution and divergence of cloned genes among bread wheat varieties that have occurred during the breeding process over the past few decades in Ningxia Province, China, are poorly understood. Here, we report the genetic diversities of 44 important genes related to grain yield, quality, adaptation and resistance in 121 Ningxia and 86 introduced wheat cultivars and advanced lines. Results The population structure indicated characteristics of genetic components of Ningxia wheat, including landraces of particular genetic resources, introduced varieties with rich genetic diversities and modern cultivars in different periods. Analysis of allele frequencies showed that the dwarfing alleles Rht-B1b at Rht-B1 and Rht-D1b at Rht-D1, 1BL/1RS translocation, Hap-1 at GW2-6B and Hap-H at Sus2-2B are very frequently present in modern Ningxia cultivars and in introduced varieties from other regions but absent in landraces. This indicates that the introduced wheat germplasm with numerous beneficial genes is vital for broadening the genetic diversity of Ningxia wheat varieties. Large population differentiation between modern cultivars and landraces has occurred in adaptation genes. Founder parents carry excellent allele combinations of important genes, with a higher number of favorable alleles than modern cultivars. Gene flow analysis showed that six founder parents have greatly contributed to breeding improvement in Ningxia Province, particularly Zhou 8425B, for yield-related genes. Conclusions Varieties introduced from other regions with rich genetic diversity and landraces with well-adapted genetic resources have been applied to improve modern cultivars. Founder parents, particularly Zhou 8425B, for yield-related genes have contributed greatly to wheat breeding improvement in Ningxia Province. These findings will greatly benefit bread wheat breeding in Ningxia Province as well as other areas with similar ecological environments.

scholarly journals Functional Gene Assessment of Wheat: Breeding Implication in Ningxia Province

2020 ◽  
Author(s):  
Weijun Zhang ◽  
Junjie Zhao ◽  
Jinshang He ◽  
Ling Kang ◽  
Xiaoliang Wang ◽  
...  
Keyword(s):  
Population Structure ◽  
Large Population ◽  
Wheat Breeding ◽  
Wheat Cultivars ◽  
Wheat Varieties ◽  
Favorable Alleles ◽  
The Past ◽  
Genetic Components ◽  
Yield Quality ◽  
Genetic Distribution

Abstract Background: The overall genetic distribution and divergences for cloned genes among wheat varieties that occurred during the breeding process of the past few decades in Ningxia province of China are poorly understood. Here we report the genetic diversities of 44 important genes underpinning grain yield, quality, adaptation and resistance in 121 Ningxia and 86 introduced wheat cultivars and advanced lines.Results: Population structure indicated characteristics of genetic components of Ningxia wheats including landraces of particular genetic resources, introduced varieties with rich genetic diversities and modern cultivars in different times. Analysis of allele frequencies showed that dwarfing alleles Rht-B1b at Rht-B1 and Rht-D1b at Rht-D1 , 1BL/1RS translocation, Hap-1 at GW2-6B and Hap-H at Sus2-2B are present very frequently in Ningxia modern cultivars and introduced varieties from other regions, but absent in landraces, indicating that the introduced wheat germplasm with numerous beneficial genes are vital for broadening genetic diversities of Ningxia wheat varieties. Large population differentiation occurred at adaptation genes between modern cultivars and well-adapted landraces. Founder parents have excellent allele combinations of important genes with a higher number of favorable alleles compared with modern cultivars. The gene flows manifested that six founder parents greatly contributed to breeding improvement in Ningxia province, in particular Zhou 8425B for yield related genes.Conclusions: These results will greatly benefit for wheat breeding in Ningxia province and other areas with similar ecological environments.

Evaluation of genetic diversity among bread wheat varieties and landraces of Pakistan by SSR markers

2009 ◽  
Vol 37 (4) ◽  
pp. 489-498 ◽  
Author(s):  
N. Iqbal ◽  
A. Tabasum ◽  
H. Sayed ◽  
A. Hameed
Keyword(s):  
Genetic Diversity ◽  
Bread Wheat ◽  
Ssr Markers ◽  
Wheat Varieties

scholarly journals Estimation of grain productivity and biochemical indicators of the winter bread wheat varieties depending on the forecrop

2021 ◽  
Vol 273 ◽  
pp. 01027
Author(s):  
Оlesya Nekrasova ◽  
Nina Kravchenko ◽  
Dmitry Marchenko ◽  
Evgeny Nekrasov
Keyword(s):  
Triticum Aestivum ◽  
Bread Wheat ◽  
Agricultural Research ◽  
Triticum Aestivum L ◽  
Wheat Breeding ◽  
Wheat Varieties ◽  
Biochemical Indicators ◽  
Winter Bread Wheat ◽  
Grain Productivity ◽  
Study Results

The purpose of the study was to estimate the effect of sunflower and pea on the amount of productivity, protein and gluten percentage in grain. The objects of the study were 13 winter bread wheat varieties (Triticum aestivum L.) developed by the Agricultural Research Center “Donskoy”. The study was carried out in 2018-2020 on the fields of the department of winter wheat breeding and seed production. The forecrops were peas and sunflower. The study results showed that the varieties ‘Volny Don’ (6.1 t / ha), ‘Krasa Dona’ (6.1 t / ha) and ‘Lidiya’ (6.0 t / ha), when sown after peas, gave the largest yields. The varieties ‘Volny Don’ (4.9 t / ha) and ‘Polina’ (4.8 t / ha) which were sown after sunflower, showed the best productivity. The analysis of qualitative indicators established that the maximum percentage of protein and gluten in grain was identified in the varieties ‘Podarok Krymu’ (16.3%; 28.3%) and ‘Volnitsa’ (16.1%; 28.5%), which were sown after peas; and the same varieties showed good results (‘Podarok Krymu’ (16.2%; 27.4%) and ‘Volnitsa’ (15.7%; 27.8%)), when sown after sunflower.

scholarly journals Investigation of Genetic Diversity in Afghan Bread Wheat Genotypes Using SSR and AFLP Markers

2019 ◽  
Vol 7 (9) ◽  
pp. 1263
Author(s):  
Mohammad Bahman Sadeqi ◽  
Said Dadshani ◽  
Mohammad Yousefi ◽  
Gul Mohammad Ajir
Keyword(s):  
Genetic Diversity ◽  
Bread Wheat ◽  
Ssr Markers ◽  
Average Distance ◽  
Wheat Breeding ◽  
Aflp Markers ◽  
Ammonium Bromide ◽  
Aflp Data ◽  
Wheat Genotypes ◽  
Diversity Assessment

Genetic diversity assessment is the principle component for conservation and characterization of germplasm. Genetic diversity study of Afghan bread wheat genotypes is a first step to identify and to select high performance genotypes and distribute to wheat breeding programs. The main objective of this study is to investigate of genetic diversity in 35 Afghan bread wheat genotypes by using Simple Sequence Repeat (SSR) and Amplified Fragment Length Polymorphism (AFLP) markers. DNA extraction according to Cetyl Trimethyl Ammonium Bromide (CTAB) method was conducted and the total genomic DNA was isolated from each variety. Sixty-four SSR primer markers were used and eighteen EcoRI+(N)/MseI+(N) primer combinations with their primer sequences were used for selective polymerase chain reaction (PCR) amplification. Every SSR and AFLP fragment was scored as present (1) or absent (0) within all genotypes under study. Marker/ Value ratio of pairwise genetic distance between genotypes according to the SSRs data was from 0.508 to 0.691 with an average distance of 0.599. Relatively different grouping pattern in comparison to AFLP data observed through cluster analysis. Both types of molecular markers (AFLP and SSR) used in this research proved to be suitable for investigating genetic diversity in the genotypes of Afghan bread wheat, however, AFLP markers gave better view of genetically relationships among genotypes than the SSR markers. The grouping generated by AFLP data showed a special agreement with the origin regions of genotypes (Ariana-07 and Mazar-99 originating from the north of Afghanistan, Lalmi-03 and Kabul-02. Large number of DNA bands identified with AFLP markers might provide a better estimation of genetic similarity than those of SSR markers.

scholarly journals An Exploration of Deep-Learning Based Phenotypic Analysis to Detect Spike Regions in Field Conditions for UK Bread Wheat

2019 ◽  
Vol 2019 ◽  
pp. 1-17 ◽  
Author(s):  
Tahani Alkhudaydi ◽  
Daniel Reynolds ◽  
Simon Griffiths ◽  
Ji Zhou ◽  
Beatriz de la Iglesia
Keyword(s):  
Deep Learning ◽  
Bread Wheat ◽  
Field Experiments ◽  
Wheat Yield ◽  
Wheat Breeding ◽  
Phenotypic Analysis ◽  
Convolutional Network ◽  
Wheat Varieties ◽  
Validation Data ◽  
Climate Conditions

Wheat is one of the major crops in the world, with a global demand expected to reach 850 million tons by 2050 that is clearly outpacing current supply. The continual pressure to sustain wheat yield due to the world’s growing population under fluctuating climate conditions requires breeders to increase yield and yield stability across environments. We are working to integrate deep learning into field-based phenotypic analysis to assist breeders in this endeavour. We have utilised wheat images collected by distributed CropQuant phenotyping workstations deployed for multiyear field experiments of UK bread wheat varieties. Based on these image series, we have developed a deep-learning based analysis pipeline to segment spike regions from complicated backgrounds. As a first step towards robust measurement of key yield traits in the field, we present a promising approach that employ Fully Convolutional Network (FCN) to perform semantic segmentation of images to segment wheat spike regions. We also demonstrate the benefits of transfer learning through the use of parameters obtained from other image datasets. We found that the FCN architecture had achieved a Mean classification Accuracy (MA) >82% on validation data and >76% on test data and Mean Intersection over Union value (MIoU) >73% on validation data and and >64% on test datasets. Through this phenomics research, we trust our attempt is likely to form a sound foundation for extracting key yield-related traits such as spikes per unit area and spikelet number per spike, which can be used to assist yield-focused wheat breeding objectives in near future.

scholarly journals Ekmeklik Buğdayda Geliştirilen Resiprokal Rekombinant Kendilenmiş Hat Populasyonunda Çavdar Translokasyonu Taşıyan Hatların Yarı Bodurluk, Fotoperiyod, Vernalizasyon ve Waxy Genlerinin Taraması

2020 ◽  
Vol 8 (4) ◽  
pp. 912-919
Author(s):  
Tuğba Güleç ◽  
Nevzat Aydın
Keyword(s):  
Bread Wheat ◽  
Recombinant Inbred ◽  
Recombinant Inbred Lines ◽  
Genetic Material ◽  
Molecular Data ◽  
Inbred Lines ◽  
Wheat Breeding ◽  
Wheat Varieties ◽  
Vrn Genes ◽  
Water Holding

The aim of this study is to screen the recombinant inbred lines (RILs) carrying rye translocation for the semi-dwarf (Rht), photoperiod (Ppd) and vernalization (Vrn) genes. Recombinant inbred lines were obtained by hybridization of bread wheat varieties Tosunbey and Tahirova-2000. Only the lines carrying 1BL.1RS rye translocation from population were used in the study. Parents and all of RILs were found to have Rht-B1a, which is a semi-dwarf allele, and Ppd-D1a allele, which is not susceptible to photoperiod. The vernalization alleles (Vrn-A1, Vrn-B1 and Vrn-D1) in the lines were also determined. It was determined that both parents and all the RILs had vrn-A1 allele, and not Vrn-A1c allele. It was determined that 151 of RILs contained vrn-B1 allele and 154 of them contained Vrn-B1 allele, while 131 of RILs had vrn-D1 allele and 174 of them had Vrn-D1 allele. In addition, molecular screening were carried out for waxy alleles (Wx-A1, Wx-B1 and Wx-D1) which were effective on flour swelling, water holding capacity and shelf life of bread wheat. As a result of the molecular data it was found that 141 of RILs carry all three alleles and named as “normal amylose wheat” and 164 of them had only Wx-A1 and Wx-D1 alleles, as known “low amylose wheat”. The results indicated that the population used in the research could be the subject of some researches, especially drought tolerance, and RILs could be used to develop genetic material carrying rye translocation for wheat breeding programs.

scholarly journals Introducing Beneficial Alleles from Plant Genetic Resources into the Wheat Germplasm

Biology ◽  
2021 ◽  
Vol 10 (10) ◽  
pp. 982
Author(s):  
Shivali Sharma ◽  
Albert Schulthess ◽  
Filippo Bassi ◽  
Ekaterina Badaeva ◽  
Kerstin Neumann ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Genetic Resources ◽  
Plant Genetic Resources ◽  
Wheat Breeding ◽  
Breeding Programs ◽  
Selection Processes ◽  
Starting Point ◽  
The Status ◽  
Modern Wheat ◽  
Elite Germplasm

Wheat (Triticum sp.) is one of the world’s most important crops, and constantly increasing its productivity is crucial to the livelihoods of millions of people. However, more than a century of intensive breeding and selection processes have eroded genetic diversity in the elite genepool, making new genetic gains difficult. Therefore, the need to introduce novel genetic diversity into modern wheat has become increasingly important. This review provides an overview of the plant genetic resources (PGR) available for wheat. We describe the most important taxonomic and phylogenetic relationships of these PGR to guide their use in wheat breeding. In addition, we present the status of the use of some of these resources in wheat breeding programs. We propose several introgression schemes that allow the transfer of qualitative and quantitative alleles from PGR into elite germplasm. With this in mind, we propose the use of a stage-gate approach to align the pre-breeding with main breeding programs to meet the needs of breeders, farmers, and end-users. Overall, this review provides a clear starting point to guide the introgression of useful alleles over the next decade.

The impact of breeding on genetic diversity and erosion in bread wheat

2005 ◽  
Vol 3 (3) ◽  
pp. 391-399 ◽  
Author(s):  
Paolo Donini ◽  
John R. Law ◽  
Robert M. D. Koebner ◽  
James C. Reeves ◽  
Robert J. Cooke
Keyword(s):  
Genetic Diversity ◽  
Bread Wheat ◽  
Morphological Characteristics ◽  
Wheat Varieties ◽  
Time Period ◽  
Ssr Loci ◽  
Genetic Pool ◽  
The Uk ◽  
Dna Microsatellite ◽  
The Impact

This paper examines the fate of alleles and changes of genetic diversity in old (ca 1930s) versus more modern (ca 1990s) UK bread wheat varieties using 14 mapped DNA microsatellite (simple sequence repeat, SSR) loci and morphological markers. The allelic constitution of varieties belonging to three time periods (early, intermediate, late) was determined. While at certain loci one or more SSR alleles were gained between early and late periods, at others the allelic representation remained constant, although a shift in allelic frequencies could sometimes be detected. No locus showed a clear, net loss in the total number of alleles over the time period. In a further group of loci, there was neither clear gain nor loss, but rather a dynamic flux of alleles. A comparison of the allelic constitution of the UK variety set with a larger genetic pool (non-UK varieties) showed that some loci were rather similar in allelic constitution, while others possessed additional diversity. Certain SSR alleles appeared to be associated with old or modern varieties, possibly indicating associations with chromosome regions under selection pressure. The same exercise was conducted on the basis of 14 of the morphological characteristics recorded in the course of distinctness, uniformity and stability testing of varieties. Overall, this analysis generated a similar picture of changes in diversity to that obtained from the microsatellite data.

scholarly journals STUDYING GENETIC RESOURCES OF SPRING BREAD WHEAT IN THE ENVIRONMENTS OF NORTHERN KAZAKHSTAN

2020 ◽  
Vol 180 (4) ◽  
pp. 44-47
Author(s):  
A. T. Babkenov ◽  
S. A. Babkenova ◽  
E. K. Kairzhanov
Keyword(s):  
Genetic Resources ◽  
Protein Content ◽  
Bread Wheat ◽  
Large Scale ◽  
Plant Genetic Resources ◽  
Sodium Dodecyl ◽  
Scale Production ◽  
Spring Bread Wheat ◽  
Wheat Varieties ◽  
Northern Kazakhstan

Background. Spring bread wheat is the main export crop in Kazakhstan. Unfortunately, wheat varieties cultivated for large-scale production do not fully meet the requirements of agricultural producers. The world diversity of wheat genetic resources should be widely used in breeding programs in order to develop new wheat cultivars with stable yields and with resistance to adverse environmental factors.Materials and methods. One hundred collection accessions of spring bread wheat were studied in 2015–2017 at the A.I. Barayev Science and Production Center of Grain Farming, Ltd. Seeds were sown at an optimum time (May 20–25), using an SSFC-7 seeder. Harvesting was conducted with a Wintersteiger combine. The study of the collection material was carried out in accordance with the guidelines developed by the N.I. Vavilov Institute of Plant Genetic Resources (VIR). The protein content was measured in line with State Standard 10846-91. The method of sodium dodecyl sulfate (SDS) sedimentation, modified by V. M. Bebyakin and M. V. Buntina, was used to measure the level of sedimentation.Results and conclusion. During the three-year study of spring bread wheat accessions in Northern Kazakhstan, only the cultivars ‘Shortandinskaya 2012’ and ‘Astana 2’ exceeded the reference ‘Astana’ in yield. The accessions ‘BW 252’, ‘Neepawa’ (Canada), ‘MANITUOU LR 13’ (CIMMYT, Mexico) and ‘Novosibirskaya 29’ (Russia) ripened 1–2 days earlier than the reference, while their average yield for 3 years was almost on the same level with the reference. The cultivars ‘Astana’ (the reference, Kazakhstan), ‘WA007824 WA7824’ (USA), ‘Novosibirskaya 29’, ‘Novosibirskaya 15’ (Russia), ‘OPATA85 LR10’ and ‘LR27+LR31,LR34’ (CIMMYT, Mexico) were distinguished for grain quality due to their high protein content and the level of sedimentation.

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