scholarly journals Soil applied glycine betaine with Arbuscular mycorrhizal fungi reduces chromium uptake and ameliorates chromium toxicity by suppressing the oxidative stress in three genetically different Sorghum (Sorghum bicolor L.) cultivars

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Praveen Kumar
Keyword(s):  
Oxidative Stress ◽  
Grain Yield ◽  
Glycine Betaine ◽  
Block Design ◽  
Growth Stages ◽  
Chromium Toxicity ◽  
Yield Attributes ◽  
Cr Toxicity ◽  
Chromium Uptake ◽  
Cr Uptake

Abstract Background Chromium is the most toxic pollutant that negatively affects a plant’s metabolic activities and yield. It reduces plant growth by influencing the antioxidant defence system’s activities. In the present study, a completely randomized block design experiment with three plants/pot in three replication was conducted on three varieties of sorghum viz. SSG 59–3, HJ 513 (multi-cut) and HJ 541 (single-cut) for amelioration of chromium toxicity (2 & 4 ppm) by exogenous application of GB (50 & 100 mM) with and without AMF in soil. The ameliorative effects were tested at two growth stages viz. vegetative (35 DAS) and grain filling (95 DAS), in terms of Cr uptake, grain yield, antioxidative defence system parameters (viz. enzymes – SOD, APX, CAT, GR, POX and metabolites – proline, glutathione, ascorbate, β-carotene) and indices of oxidative stress parameters (viz. PPO, H2O2, and MDA). Results The results delineated that Cr uptake and indices of oxidative stress were increased with increasing concentration of Cr stress in all the varieties (HJ 541, HJ513 & SSG 59–3) at both the growth stages (35 & 95 DAS). At higher concentration (4 ppm), Cr stress decreased the grain yield (45–50%) as compared with controls. Polyphenoloxidase activity, MDA and H2O2 content increased at both growth stages in all the varieties. However, antioxidative enzymes and metabolite activities increased due to Cr stress but this increase was not sufficient to counteract with ROS generated under Cr stress which was enhanced on the application of AMF and GB either individually or in combination (spiked in soil). It decreased the indices of oxidative stress and ameliorated the Cr toxicity and increased grain yield (65–70%) in all the varieties. Conclusions Both GB and AMF improved the antioxidative activities and stress tolerance capacity of the plant. Glycine betaine at both 50 and 100 mM level, significantly ameliorated Cr toxicity. However, AMF concomitantly with GB further boosts up the amelioration behaviour of the plant against Cr toxicity, at both growth stages in all the varieties. The combination of 100 mM GB with 10 g AMF was observed most effective among all the treatments. Among the varieties, SSG 59–3 had the lowest chromium uptake, indices of oxidative stress, and highest antioxidative system’s activity as compared to HJ 513 followed by HJ 541 variety. Thus AMF and GB either individually or in combination may be used to maintain plant yield attributes under Cr toxicity.

scholarly journals Exogenous AMF with Glycine Betaine Reduces Cr (VI) Uptake and Alleviates Chromium Toxicity by Supressing the Oxidative Stress in Three Genetically Different Sorghum (Sorghum bicolor L.) Cultivars

2020 ◽  
Author(s):  
Praveen Kumar
Keyword(s):  
Oxidative Stress ◽  
Plant Growth ◽  
Grain Yield ◽  
Growth Stages ◽  
Yield Attributes ◽  
Cr Toxicity ◽  
Toxic Pollutant ◽  
Metabolic Activities ◽  
Chromium Uptake ◽  
Cr Uptake

Abstract Background: Chromium is most toxic pollutant that negatively affects plant’s metabolic activities and yield. It reduces plant growth by influencing the antioxidant defense system’s activities. The aim of the present research was to examine the ameliorative capability of exogenous GB and AMF spiked in soil, either individually or in combination against Cr toxicity. The ameliorative effects were studied in terms of Cr uptake, grain yield, antioxidative defense system parameters (viz. enzymes – SOD, APX, CAT, GR, POX and metabolites – proline, glutathione, ascorbate, β-carotene) and indices of oxidative stress parameters (viz. PPO, H2O2, and MDA). Results:The results delineated that Cr uptake and indices of oxidative stress were increased with increasing concentration of Cr stress in all the varieties (HJ 541, HJ513 & SSG 59-3) at both the growth stages (35 & 95 DAS). At higher concentration (4 ppm), Cr stress decreased the grain yield (45-50%) as compared with controls. PPO activity, MDA and H2O2 content increased at both growth stages in all the varieties. However, antioxidative enzymes and metabolite activities increased due to Cr stress but this increase was not sufficient to counteract with ROS generated under Cr stress which was enhanced on the application of AMF and GB either individually or in combination (spiked in soil). It decreased the indices of oxidative stress and ameliorated the Cr toxicity and increased grain yield (65-70%) in all the varieties. Conclusions:Both GB and AMF, improved the plant growth and stress tolerance capacity of the plant. GB at both 50 and 100 mM level, significantly ameliorated Cr (VI) toxicity. However, AMF concomitantly with GB further boost up the amelioration behavior of the plant against Cr toxicity, at both growth stages in all the varieties. The combination of 100 mM GB with 10 g AMF was observed most effective among all the treatments. Among the varieties SSG 59-3 had the lowest chromium uptake, indices of oxidative stress, and highest antioxidative system’s activity as compared to HJ 513 followed by HJ 541 variety. Thus AMF and GB either individually or in combination may be used to maintain plant yield attributes under Cr toxicity.

scholarly journals Title: The Cumulative Dose of AMF and GB is More Effective in The Amelioration of Cr(VI) Toxicity in Sorghum (Sorghum Bicolor L.) Than Individually

2021 ◽  
Author(s):  
Praveen Kumar
Keyword(s):  
Oxidative Stress ◽  
Heavy Metal ◽  
Grain Yield ◽  
Anthropogenic Activities ◽  
Growth Stages ◽  
Antioxidant Defence ◽  
Yield Attributes ◽  
Defence System ◽  
Antioxidant Defence System ◽  
Cr Toxicity

Abstract Industrial and anthropogenic activities are the major source of heavy metal toxicants in agricultural soils. Among, heavy metal toxicants, hexavalent chromium is the most toxic toxicant that negatively affects plant’s metabolic activities and yield. It reduces the plant growth and development by influencing the antioxidant defence system’s activities. In the present experiment, two different soil applied dozes of GB viz. 50 and 100mM, and AMF, both individually and in combination were tested for their capability to ameliorate Cr toxicity in sorghum. The promotive behaviour of these treatments for antioxidant defence system was analysed at vegetative (35 DAS) and grain filling stage (95 DAS) in three varieties of sorghum viz. SSG 59-3, HJ 513 (multi-cut) and HJ 541 (single-cut) under 2 and 4 ppm Cr toxicity. At the same time resultant effects of this behaviour on Cr accumulation, grain yield and indices of oxidative stress was also studied. In this experiment antioxidant defence system includes enzymes viz. SOD, APX, CAT, GR, POD and metabolites viz. glutathione, ascorbate, proline, β-carotene and indices of oxidative stress includes parameters viz. PPO, H2O2 and MDA. The results delineated that Cr accumulation and indices of oxidative stress were increased with increasing concentration of Cr stress in all the varieties at both growth stages. Chromium stress at high concentration (4 ppm), decreased the grain yield (71.69 %) as compared with control. Due to 4 ppm Cr stress, PPO activity, MDA and H2O2 accumulation increased significantly (72.29 %, 73.15 %, 79 % respectively, at 35 DAS and 70.36 %, 74.78 %, 79.83 % respectively, at 95 DAS). GB and AMF individually increased antioxidant activity but in combination, further significantly increased antioxidant defence system’s activity which in turn decreased indices of oxidative stress and reduced the Cr toxicity and increased grain yield of sorghum in all varieties at both the growth stages. However, treatment of 100mM GB with AMF was observed most significant in decreasing oxidative stress and improved antioxidant system’s activities and grain yield as compared with all other treatments at both growth stages in all the varieties. SSG 59-3 cultivar showed lowest chromium content (1.60 and 8.61 ppm), indices of oxidative stress and highest antioxidant system’s activity as compared to HJ 513 followed by HJ 541 variety, at 35 and 95 DAS respectively. Thus, among the varieties SSG 59-3 was found most tolerant as compared to HJ 513 followed by HJ 541 variety. These findings suggest that both GB and AMF, either individually or combined can play a positive role to reduce oxidative stress and increased yield attributes under Cr toxicity in sorghum.

scholarly journals Improvement yield and nitrogen uptake of wheat through application of organic and inorganic fertilizers

2016 ◽  
Vol 27 (2) ◽  
pp. 149-153
Author(s):  
MR Islam ◽  
S Siraj ◽  
A Huda ◽  
MLN Begum ◽  
S Bilkis
Keyword(s):  
Grain Yield ◽  
Block Design ◽  
N Uptake ◽  
Superior Performance ◽  
Inorganic Fertilizers ◽  
Floodplain Soil ◽  
Yield Attributes ◽  
Science Field ◽  
Straw Yield ◽  
Organic And Inorganic Fertilizers

An experiment was conducted at the Soil Science Field Laboratory of Bangladesh Agricultural University, Mymensingh during winter (Rabi) season of 2013-14 to evaluate the effect of integrated use of organic and inorganic fertilizers on the growth, yield and nitrogen (N) uptake of wheat. There were six treatments such as T0 (control), T1 [STB-CF (HYG)], T2 [STB-CF (HYG) + CD (5 t/ha)], T3 [STB-CF (HYG) +PM (3 t/ha)], T4 [STB-CF (HYG) COM (5 t/ha)] and T5 [FP (Farmers’ practice)]. The experiment was laid out in a Randomized Complete Block Design with four replications. Soil test based nitrogen, phosphorus, potassium, sulphur, zinc and boron were used @ 100, 20, 60, 10, 3 and 2 kg ha-1, respectively. The integrated use of organic and inorganic fertilizers significantly increased the yield attributes as well as grain and straw yields of wheat. The treatment T3 [STB-CF (HYG) + PM (3 tha-1)] produced the highest grain yield of 3554 kg ha-1 (69.67% increase over control) and straw yield of 3635 kg ha-1 (62.83% increase over control). The lowest grain yield (2094 kg ha-1) and straw yield (2232 kg ha-1) were found in control treatment. The N content and uptake by wheat were also markedly influenced by combined use of organic and inorganic fertilizers and the treatment T3 demonstrated superior performance to other treatments. So, the applications of fertilizers in combination with poultry manure @ 3 t ha-1 can be used for the successful cultivation of wheat in Old Brahmaputra Floodplain soil. Progressive Agriculture 27 (2): 149-153, 2016

Effect of pink-pigmented facultative methylotrophs, PGRs and Nutrients on Growth, Yield and Economics of Irrigated Blackgram [Vigna mungo (L.) Hepper]

2021 ◽  
Author(s):  
R. Ajaykumar ◽  
S. Selvakumar ◽  
K. Harishankar ◽  
K. Sivasabari
Keyword(s):  
Grain Yield ◽  
Nutritive Value ◽  
Foliar Application ◽  
Positive Impact ◽  
Block Design ◽  
Growth Yield ◽  
Vigna Mungo ◽  
Area Index ◽  
Yield Attributes ◽  
Randomized Block Design

Background: Blackgram [Vigna mungo (L.) Hepper] is one of the most important cultivated legume crops with high nutritive value and agricultural importance. The productivity of blackgram is not adequate to meet the domestic demand of the growing Indian population. Consequently, there is an urgent need for enhancement of productivity through proper agronomic practices. With this background, a research experiment was conducted in a farmer’s field at Pudhupalayam, Coimbatore to investigate the effect of foliar application of PPFM, plant growth regulating compounds and nutrients on growth, yield attributes, yield and economics of irrigated blackgram. Methods: A field experiment was laid out in randomized block design with three replications during 2019 (kharif and rabi) season. The treatments include 100% recommended dose of NPK along with foliar application of diammonium phosphate (DAP), brassinolide (Br), salicylic acid (SA) and pink pigmented facultative methylotrophs (PPFM) with different concentration (1%, 2%, 1 ppm, 2 ppm, 50 ppm, 100 ppm, respectively) in addition to control. PPFM and PGRs were sprayed at 30 and 45 days after the sowing of blackgram. Result: The experiment results of the two seasons study revealed that the application of 100% RDF + PPFM @ 2% recorded higher growth characters viz., plant height (cm), number of branches plant-1, leaf area index and yield attributes viz., number of pods plant-1, number of seeds pod-1, pod weight (g), pod height (cm), 100 seed weight (g) and yield viz., grain yield (kg/ha), straw yield (kg/ha) and harvest index (%). As well as the same treatment recorded higher net return and B:C ratio. Correlation and regression analysis also indicated that the yield attributes had a positive impact on the grain yield with a magnitude of 1.91 and 1.67, respectively. Therefore, application of 100% RDF+2% PPFM spray can be recommended as the best technology to improve the yield and economics of blackgram.

scholarly journals Direct and Residual Effect of Sulphur and Zinc on Potato-Boro-T. Aman Rice Cropping Pattern

2013 ◽  
Vol 19 (1) ◽  
pp. 33-38 ◽  
Author(s):  
MA Tarafder ◽  
MQ Haque ◽  
MM Rahman ◽  
MR Khan
Keyword(s):  
Grain Yield ◽  
Sandy Loam ◽  
Block Design ◽  
Residual Effect ◽  
Growth And Yield ◽  
Residual Effects ◽  
Cropping Pattern ◽  
Yield Attributes ◽  
The Third ◽  
Boro Rice

The experiment was conducted at the Bangladesh Institute of Nuclear Agriculture (BINA) sub-staion, Tajhat, Ranpure, using potato-Boro-T. aman rice cropping pattern with an objective to evaluate the direct and residual effects of sulphur and zinc on the growth, yield and nutrient uptake by the crops. The surface soil was sandy loam texture, pH 6.3, organic matter 1.24%, available sulphur 6.62 ppm and available zinc 0.45 ppm. The experiment comprised of eight treatments for potato S15Zn2 (T2, T4 and T8, S8Zn1 (T5 and T6)and S0Zn0 (T1, T3 and T7), for boro rice S20Zn4 (T3, T5, T6 and T7) and S0Zn0 (T1, T3, T4 and T6). The experiment was laid out in a randomized complete block design with three replications. Average tuber yield of potato (var. cardinal) varied from 28.29 to 32.86 t ha-1 with the highest yield in S15Zn2 treatment (100% recommended dose) and the lowest was in the S0Zn0 treatment (control). In the second crop (Boro rice), growth and yield attributes, grain and straw yields responded significantly to S and Zn applied either in the first crop or in both crops. The average grain yield varied from 3.51 to 5.27 t ha-1 over the treatments. In the third crop (T. aman rice), the grain and straw yields responded significantly to S and Zn applied either in the first and second crop or in the third crops. The grain yield of T. aman rice varied from 2.96 to 4.46 t ha-1 over the treatments. The growth and yield contributing characters were also significantly influenced by the treatment. There was a significant direct and residual effect of the treatments on S and Zn uptake by the crops.DOI: http://dx.doi.org/10.3329/pa.v19i1.16987 Progress. Agric. 19(1): 33 - 38, 2008 

Appropriate Nitrogen and Phosphorus Fertilizer Regime for Sunflower (Helianthus Annuus L.) In the Humid Tropics

Helia ◽  
2019 ◽  
Vol 42 (70) ◽  
pp. 111-125
Author(s):  
E. Akpojotor ◽  
V.I.O. Olowe ◽  
C. Adejuyigbe ◽  
S.O. Adigbo
Keyword(s):  
Grain Yield ◽  
Plant Height ◽  
Agricultural Research ◽  
Block Design ◽  
Field Trials ◽  
Humid Tropics ◽  
Single Application ◽  
Nitrogen And Phosphorus ◽  
Yield Attributes ◽  
P Fertilizer

AbstractTwo field trials were conducted on the Research Farm of the Institute of Food Security, Environmental Resources and Agricultural Research, Nigeria during the late cropping seasons (Jun.–Nov.) of 2014 and 2015 to evaluate the agronomic performance of four recently released sunflower varieties (SAMSUN-1, SMASUN-2, SAMSUN-3 and SAMSUN-4) to three fertilizer regimes: Control, Split application of 30 kg N + 28 kg P2O5 at 21 days after sowing (DAS) and at anthesis and Single application of 60 kg N and 56 kg P2O5 at 21DAS. The experiment was laid out in a randomized complete block design using a 3 × 4 factorial arrangement and replicated three times. Data were collected on phenology, plant height, seed yield and yield attributes, and quality. The varietal effect was only significant in 2015 for head weight, a number of achene per head and 100 achene weight. Application of N and P fertilizer either as split or single significantly (P ≤ 0.05; F-test) enhanced plant height at R5 and R9, 100 achene weight, achene weight per head and grain yield in both years. Single application resulted in significantly (P ≤ 0.05) higher grain yield in 2014 than the split and control and was on par with a split. Significant variety × fertilizer regime was recorded for protein content in 2014 and 2015, and oil content in 2015. Therefore, a single application of N and P fertilizers at 21 WAS is recommended for adoption in the humid tropics to enhance seed and oil production of SAMSUN-3 and SAMSUN-4.

scholarly journals Effect of Microelements and Selenium on Superoxide Dismutase Enzyme, Malondialdehyde Activity and Grain Yield Maize (Zea mays L.) under Water Deficit Stress

2011 ◽  
Vol 39 (2) ◽  
pp. 153 ◽  
Author(s):  
Nourali SAJEDI ◽  
Hamid MADANI ◽  
Ahmad NADERI
Keyword(s):  
Superoxide Dismutase ◽  
Water Stress ◽  
Grain Yield ◽  
Water Deficit ◽  
Block Design ◽  
Stress Conditions ◽  
Water Deficit Stress ◽  
Growth Stages ◽  
Malondialdehyde Content ◽  
Main Plot

This study was carried out to investigate effects of microelements under water deficit stress at different growth stages on antioxidant enzyme alteration, chemical biomarker and grain yield of maize in the years 2007 and 2008. The experiment was conducted in a split plot factorial based on a randomized complete block design with four replications. There were three factors, water deficit stress at different stages of growth as main plot and combinations of selenium (with and without using) and microelements (with and without using) as sub plots. The result indicated that the activity of superoxide dismutase and malondialdehyde content under water deficit stress increased, but grain yield was reduced. The highest grain yield was obtained from optimum irrigation, while in the case of with water deficit stress at V8 stage it was non significant. Selenium spray increased activity of superoxide dismutase enzyme, malondialdehyde content of leaves in V8, R2 and R4 stages and also grain yield. Application of microelements increased the leaves superoxide dismutase enzyme activity and malondialdehyde content. Selenium and microelements spray under water deficit stress conditions during vegetative growth and dough stage increased grain yield in comparison to not spraying elements under water stress conditions. The present results also showed that by using selenium and microelements under water stress can obtain acceptable yield compared to not using these elements.

scholarly journals Foliar Nitrogen Management for Improving Growth and Yield of Dryland Wheat

2015 ◽  
Vol 48 (3) ◽  
pp. 23-31 ◽  
Author(s):  
◽  
I. Alam ◽  
I. Khan ◽  
M. Kumar ◽  
A. Shah ◽  
...  
Keyword(s):  
Grain Yield ◽  
Yield Components ◽  
Block Design ◽  
Foliar Spray ◽  
Growth And Yield ◽  
Growth Stages ◽  
Sowing Time ◽  
N Application ◽  
Foliar Nitrogen ◽  
Foliar N

Abstract Foliar nitrogen (N) application is considered an important factor affecting phenology, growth, yield, and yield components of dryland wheat (Triticum aestivum L.). A field experiment was conducted to study effects of foliar N on dryland wheat (cv. Prisabk-2004) at the Agronomy Research Farm, The University of Agriculture Peshawar, Pakistan, during winter 2010-2011. The experiment was laid out in randomized complete block design using four replications. A plot size of 3 m by 4 m, having 8 rows, 4 m long and 30 cm apart was used. A total of 80 kg N ha−1 in the form of urea was applied. Out of 80 kg N ha−1, 70 kg N ha−1 was applied to the soil at sowing time, and the remaining 10 kg N ha−1 was applied in the form of foliar spray (2% N). The required foliar N was applied in various combinations (splits) at different growth stages viz. 30, 60, 90 and 120 days after emergence (DAE). Phenological development (days to anthesis and physiological maturity) was delayed, yield components and yield increased significantly (p ≤ 0.05) with foliar N over control (water spray without N). Wheat grain yield increased to the highest level (4427 kg ha−1) when 100 % foliar N was applied (no split) at 90 DAE, followed by 4050 kg ha−1 at 120 DAE, while the control (no foliar N) resulted in the lowest grain yield (2573 kg ha−1). We concluded from this study that 2 % foliar N application in a single split either at 90 or 120 DAE could improve wheat productivity under dryland condition.

scholarly journals Effect of Real time nitrogen management on yield attributes of direct seeded rice by using SPAD meter and Greenseeker

2021 ◽  
Author(s):  
saishree HALLE ◽  
SABYASACHI BISWAL ◽  
BOYAPATI RAVI TEJA NAIDU ◽  
REDDYYAMINI BOMMISETTY
Keyword(s):  
Grain Yield ◽  
Dry Matter ◽  
Block Design ◽  
Rice Variety ◽  
Threshold Value ◽  
Nitrogen Management ◽  
Yield Attributes ◽  
Randomized Block Design ◽  
Direct Seeded ◽  
Spad Meter

A field experiment was conducted during Kharif 2018, laid out in Randomized Block Design with three replications having seven treatments viz. N omission (T1), N applied as basal and AT (T2), N as basal, AT and PI (T3), N as basal and top dressing at NDVI threshold of 0.75 (T4), at NDVI threshold of 0.8 (T5), at SPAD threshold of 35.0 (T6) and SPAD threshold of 37.5 (T7) with Rice variety Sahabhagidhan.The study revealed that application of 30 kg N/ha as basal dose and top dressing of 20 kg N/ha twice at 35 and 63 DAS guided by NDVI threshold value of 0.8 (T5) was found to be superior over other treatments with respect to productivity. T5 recorded highest grain yield of 4438 kg/ha which was 17.0% higher than that top dressed at NDVI threshold of 0.75 (T4) and 7.1% higher than that top dressed at SPAD threshold value of 37.5 (T7). In case of SPAD meter, nitrogen top dressed at threshold value of 37.5 (T7) produced grain yield of 4143 kg/ha which was 15.0% higher than T6. T5 produced maximum dry matter of 8678 kg/ha with highest grain yield (4438 kg/ha), straw yield (5092 kg/ha) and harvest index 46.0%.

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