MYB transcription factor family in sweet cherry (Prunus avium L.): genome-wide investigation, evolution, structure, characterization and expression patterns

Abstract Back ground MYB Transcription factors (TFs) are most imperative and largest gene family in plants, which participate in development, metabolism, defense, differentiation and stress response. The MYB TFs has been studied in various plant species. However, comprehensive studies of MYB gene family in the sweet cherry (Prunus avium L.) are still unknown. Results In the current study, a total of 69 MYB genes were investigated from sweet cherry genome and classified into 28 subfamilies (C1-C28 based on phylogenetic and structural analysis). Microcollinearity analysis revealed that dispersed duplication (DSD) events might play an important role in the MYB genes family expansion. Chromosomal localization, the synonymous (Ks) and nonsynonymous (Ka) analysis, molecular characteristics (pI, weight and length of amino acids) and subcellular localization were accomplished using several bioinformatics tools. Furthermore, the members of distinct subfamilies have diverse cis-acting regions, conserved motifs, and intron-exon architectures, indicating functional heterogeneity in the MYB family. Moreover, the transcriptomic data exposed that MYB genes might play vital role in bud dormancy. The quantitative real-time qRT-PCR was carried out and the expression pattern indicated that MYB genes significantly expressed in floral bud as compared to flower and fruit. Conclusion Our comprehensive findings provide supportive insights into the evolutions, expansion complexity and functionality of PavMYB genes. These PavMYB genes should be further investigated as they seem to be brilliant candidates for dormancy manipulation in sweet cherry.

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MYB Transcription Factor Family in Sweet Cherry (Prunus avium L.): Genome-wide Investigation, Evolution, Structure, Characterization and Expression Patterns

10.21203/rs.3.rs-783177/v1 ◽

2021 ◽

Author(s):

Irfan Ali Sabir ◽

Muhammad Aamir Manzoor ◽

Iftikhar Hussain Shah ◽

Xunju Liu ◽

Muhmmad Salman Zahid ◽

...

Keyword(s):

Gene Family ◽

Sweet Cherry ◽

Prunus Avium ◽

Functional Divergence ◽

Expression Patterns ◽

Structure Characterization ◽

Myb Transcription Factor ◽

Molecular Characteristics ◽

Myb Genes ◽

Prunus Avium L

Abstract Back groundMYB Transcription factors (TFs) are most imperative and largest gene family in plants, which participate in development, metabolism, defense, differentiation and stress response. The MYB TFs has been studied in various plant species. However, comprehensive studies of MYB gene family in the sweet cherry (Prunus avium L.) are still unknown. ResultsIn the current study, a total of 69 MYB genes were investigated from sweet cherry genome and classified into 28 subfamilies (C1-C28 based on phylogenetic and structural analysis). Microcollinearity analysis revealed that dispersed duplication (DSD) and whole-genome duplication (WGD) events might play an important role in the expansion of the MYB genes family. Chromosomal localization, the synonymous (Ks) and nonsynonymous (Ka) analysis, molecular characteristics (pI, weight and length of amino acids) and subcellular localization were accomplished using several bioinformatics tools. Moreover, different subfamilies contained different cis-acting elements, conserved motifs analysis and introns-exons structures, representing functional divergence in the MYB family. Subsequently, the transcriptomic data exposed that MYB genes might play vital role in bud dormancy. Subsequently, the quantitative real-time qRT-PCR was carried out and the expression pattern indicated that MYB genes significantly expressed in floral bud as compared to flower and fruit. ConclusionOur comprehensive findings provide supportive insights into the evolutions, expansion complexity and functionality of PavMYB genes. These PavMYB genes should be further investigated as they seem to be brilliant candidates for dormancy manipulation in sweet cherry.

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Genome-Wide Identification of ARF Gene Family Suggests a Functional Expression Pattern during Fruitlet Abscission in Prunus avium L.

International Journal of Molecular Sciences ◽

10.3390/ijms222111968 ◽

2021 ◽

Vol 22 (21) ◽

pp. 11968

Author(s):

Qiandong Hou ◽

Zhilang Qiu ◽

Zhuang Wen ◽

Huimin Zhang ◽

Zhengchun Li ◽

...

Keyword(s):

Plant Growth ◽

Expression Pattern ◽

Sweet Cherry ◽

Prunus Avium ◽

Expression Patterns ◽

Abiotic Stress Response ◽

Drought Treatment ◽

Response Factors ◽

Prunus Avium L ◽

Fruitlet Abscission

Auxin response factors (ARFs) play a vital role in plant growth and development. In the current study, 16 ARF members have been identified in the sweet cherry (Prunus avium L.) genome. These genes are all located in the nucleus. Sequence analysis showed that genes in the same subgroup have similar exon-intron structures. A phylogenetic tree has been divided into five groups. The promoter sequence includes six kinds of plant hormone-related elements, as well as abiotic stress response elements such as low temperature or drought. The expression patterns of PavARF in different tissues, fruitlet abscission, cold and drought treatment were comprehensively analyzed. PavARF10/13 was up-regulated and PavARF4/7/11/12/15 was down-regulated in fruitlet abscising. These genes may be involved in the regulation of fruit drop in sweet cherry fruits. This study comprehensively analyzed the bioinformatics and expression pattern of PavARF, which can lay the foundation for further understanding the PavARF family in plant growth development and fruit abscission.

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Identification of the laccase-like multicopper oxidase gene family of sweet cherry (Prunus avium L.) and expression analysis in six ancient Tuscan varieties

Scientific Reports ◽

10.1038/s41598-019-39151-z ◽

2019 ◽

Vol 9 (1) ◽

Cited By ~ 4

Author(s):

Roberto Berni ◽

Emilie Piasecki ◽

Sylvain Legay ◽

Jean-Francois Hausman ◽

Khawar Sohail Siddiqui ◽

...

Keyword(s):

Gene Family ◽

Expression Analysis ◽

Sweet Cherry ◽

Prunus Avium ◽

Multicopper Oxidase ◽

Oxidase Gene ◽

Prunus Avium L

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Biochemical and phenotypic characterization of sweet cherry (Prunus avium L.) cultivars with induced surface pitting

Postharvest Biology and Technology ◽

10.1016/j.postharvbio.2021.111494 ◽

2021 ◽

Vol 175 ◽

pp. 111494

Author(s):

Excequel Ponce ◽

Blanca Alzola ◽

Natalia Cáceres ◽

Madeline Gas ◽

Catalina Ferreira ◽

...

Keyword(s):

Sweet Cherry ◽

Prunus Avium ◽

Phenotypic Characterization ◽

Prunus Avium L

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The use of differential thermal analysis in determining the critical temperatures of sweet cherry (Prunus avium L.) flower buds at different stages of bud burst

International Journal of Biometeorology ◽

10.1007/s00484-021-02093-1 ◽

2021 ◽

Author(s):

Ozkan Kaya ◽

Cafer Kose ◽

Muge Sahin

Keyword(s):

Thermal Analysis ◽

Differential Thermal Analysis ◽

Sweet Cherry ◽

Prunus Avium ◽

Bud Burst ◽

Flower Buds ◽

Critical Temperatures ◽

Prunus Avium L

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Construction of an intra-specific sweet cherry (Prunus avium L.) genetic linkage map and synteny analysis with the Prunus reference map

Tree Genetics & Genomes ◽

10.1007/s11295-008-0161-1 ◽

2008 ◽

Vol 4 (4) ◽

pp. 897-910 ◽

Cited By ~ 59

Author(s):

James W. Olmstead ◽

Audrey M. Sebolt ◽

Antonio Cabrera ◽

Suneth S. Sooriyapathirana ◽

Sue Hammar ◽

...

Keyword(s):

Linkage Map ◽

Genetic Linkage ◽

Sweet Cherry ◽

Genetic Linkage Map ◽

Prunus Avium ◽

Synteny Analysis ◽

Reference Map ◽

Prunus Avium L

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PaCYP78A9, a Cytochrome P450, Regulates Fruit Size in Sweet Cherry (Prunus avium L.)

Frontiers in Plant Science ◽

10.3389/fpls.2017.02076 ◽

2017 ◽

Vol 8 ◽

Cited By ~ 13

Author(s):

Xiliang Qi ◽

Congli Liu ◽

Lulu Song ◽

Yuhong Li ◽

Ming Li

Keyword(s):

Cytochrome P450 ◽

Sweet Cherry ◽

Prunus Avium ◽

Fruit Size ◽

Prunus Avium L

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Variability of Old Sweet Cherries Found in Slovak Regions and Their Preservation

Proceedings of the Latvian Academy of Sciences Section B Natural Exact and Applied Sciences ◽

10.1515/prolas-2017-0031 ◽

2017 ◽

Vol 71 (3) ◽

pp. 184-189

Author(s):

Michaela Benková ◽

Iveta Čičová ◽

Daniela Benedikova ◽

Lubomir Mendel ◽

Miroslav Glasa

Keyword(s):

Sweet Cherry ◽

Prunus Avium ◽

Fruit Size ◽

Morphological Characteristics ◽

Quality Parameters ◽

Fruit Weight ◽

Sweet Cherries ◽

Prunus Mahaleb ◽

Breeding Programmes ◽

Prunus Avium L

Abstract The work is focused on the evaluation of variability of morphological and pomological characteristics of several old sweet cherries (Prunus avium L.) that were found in different Slovak regions. The experimental work has been performed during two years, 2014 and 2015. The following characteristics according to the descriptor list of subgenus Cerasus were evaluated - period of flowering and ripening, morphological characteristics of the flowers, fruit size, fruit weight, and quality parameters. The results showed high variability of evaluated accessions. From the 13 surveyed localities, the most valuable accessions were found in the locality Hornį Streda - places Čachtice, Krakovany, Nitra, and Brdárka. During the collecting expeditions, 170 accessions of sweet cherry, with fruit of the different quality were found. The most interesting accessions were grafted onto rootstocks with different intensity of growth (Prunus avium L., Prunus mahaleb L., and ‘Gisela5’). Some of the selected cherry accessions can be used for commercial growing after tests, while some of them can be used only for collection of genetic resources and as potential genitors in breeding programmes.

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INSECT TRANSMISSION OF THE VIRUS CAUSING LITTLE CHERRY DISEASE

Canadian Journal of Plant Science ◽

10.4141/cjps60-103 ◽

1960 ◽

Vol 40 (4) ◽

pp. 707-712 ◽

Cited By ~ 6

Author(s):

W. H. A. Wilde

Keyword(s):

Sweet Cherry ◽

Prunus Avium ◽

Virus Disease ◽

Insect Transmission ◽

Prunus Avium L ◽

Cherry Trees

Little cherry virus disease of sweet cherry (Prunus avium L.) was transmitted under screenhouse conditions by 3 species of leafhoppers (Homoptera: Cicadellidae) out of 24 species tested. Macrosteles fascifrons (Stal), the 6-spotted leafhopper, transmitted the disease in seven tests; Scaphytopius acutus (Say), the sharp-nosed leafhopper, transmitted it once; and Psammotettix lividellus (Zett.) transmitted it once. The transmissions were made from diseased sweet cherry trees of the variety Lambert to indicators of the varieties Star or Sam. With the exception of 1 transmission, 2 to 4 years were necessary following inoculation for unmistakable expression of symptoms in the indicators. M. fascifrons was also implicated in 18 successful transmissions to mature sweet cherry trees grown in the open.

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