scholarly journals The expression and clinical significance of miR-1226 in patients with periodontitis

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Yimin Du ◽  
Yue-sun Qi ◽  
Hui Chen ◽  
Guorong Shen
Keyword(s):  
Healthy Volunteers ◽  
Clinical Significance ◽  
Inflammatory Cytokines ◽  
Gingival Crevicular Fluid ◽  
Pearson Correlation ◽  
High Sensitivity ◽  
High Concentration ◽  
Pocket Depth ◽  
Crevicular Fluid ◽  
Pro Inflammatory Cytokines

Abstract Background miR-1226 has been reported to be dysregulated in periodontitis, implying its potential functional role, which needs to be validated. The purpose of this study was to assess the clinical significance of miR-1226 in periodontitis. Methods Gingival crevicular fluid samples were collected from 50 healthy volunteers and 72 periodontitis patients. The expression of miR-1226 in collected samples was detected by RT-qPCR. The concentrations of pro-inflammatory cytokines were analyzed by ELISA. The relationship of miR-1226 expression level with patients’ characteristics was evaluated by the χ2 test and the Pearson correlation test. Results It was found that miR-1226 was downregulated in the gingival crevicular fluid of periodontitis patients compared with healthy volunteers. The downregulation of miR-1226 was negatively correlated with the pocket depth, attachment loss, plaque index, bleeding index, and MMP-8 concentration of patients. miR-1226 showed high sensitivity and specificity to discriminate periodontitis patients from healthy volunteers. Additionally, periodontitis patients had a relatively high concentration of pro-inflammatory cytokines, which is correlated with miR-1226 expression negatively. Conclusions miR-1226 could be an indicator for the diagnosis of periodontitis and has the potential to predict the development and severity of periodontitis.

The relationship between oral mucositis and levels of pro-inflammatory cytokines in serum and in gingival crevicular fluid in allogeneic stem cell recipients

2014 ◽  
Vol 23 (6) ◽  
pp. 1749-1757 ◽  
Author(s):  
Karin Garming Legert ◽  
Georgios Tsilingaridis ◽  
Mats Remberger ◽  
Olle Ringdèn ◽  
Anders Heimdahl ◽  
...  
Keyword(s):  
Stem Cell ◽  
Inflammatory Cytokines ◽  
Oral Mucositis ◽  
Gingival Crevicular Fluid ◽  
Crevicular Fluid ◽  
The Relationship ◽  
Pro Inflammatory Cytokines

scholarly journals Variation of the Cytokine Profiles in Gingival Crevicular Fluid Between Different Groups of Periodontally Healthy Teeth

2019 ◽  
Vol 0 (0) ◽  
Author(s):  
Ervin Taso ◽  
Mia Rakic ◽  
Vladimir Stefanovic ◽  
Aleksandra Petković-Curcin ◽  
Ivan Stanojevic ◽  
...  
Keyword(s):  
Bone Resorption ◽  
Inflammatory Cytokines ◽  
Gingival Crevicular Fluid ◽  
Posterior Teeth ◽  
Periodontal Tissues ◽  
Cytokine Profiles ◽  
Anterior Teeth ◽  
Cytokine Levels ◽  
Crevicular Fluid ◽  
Pro Inflammatory Cytokines

Abstract Profiling of biomarkers of physiological process represents an integrative part in optimisation of diagnostic markers in order to adjust the diagnostic ranges to the potential effects of the local factors such occlusal forces in case of periodontal tissues. The objective of this study was estimation of IL-1β, IL-2, IL-4, IL-5, IL-6, IL-9, IL-10, IL-12, IL-13, IL-17, IL-22, TNFα and IFNγ concentrations in gingival crevicular fluid samples (GCF) between different groups of teeth. Two hundred fifty-nine systemically healthy non-smokers having at least one vital tooth without restorations, with healthy periodontal tissues, were clinically examined and the GCF sample was retrieved. The cytokine levels were estimated using flow cytometry and compared between central incisors (CI), lateral incisors, canines, first premolars, second premo-lars, first molars and second molars. Cytokine profiles varied between different groups of teeth with tendency of increase in pro-inflammatory cytokines from anterior teeth toward molars. Molars might be considered teeth with natural predisposition for faster bone resorption while the adjustment of diagnostic range of periodontal biomarkers for anterior or posterior teeth should be considered within diagnostic context. Cytokine profiles varied between different groups of teeth with tendency of increase in pro-inflammatory cytokines from anterior teeth toward molars. Molars might be considered teeth with natural predisposition for faster bone resorption while the adjustment of diagnostic range of periodontal biomarkers for anterior or posterior teeth should be considered within diagnostic context.

scholarly journals Origin of MMP-8 and Lactoferrin levels from gingival crevicular fluid, salivary glands and whole saliva

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Liza L. Ramenzoni ◽  
Deborah Hofer ◽  
Alex Solderer ◽  
Daniel Wiedemeier ◽  
Thomas Attin ◽  
...  
Keyword(s):  
Matrix Metalloproteinase ◽  
Salivary Glands ◽  
Gingival Crevicular Fluid ◽  
Clinical Parameters ◽  
Pocket Depth ◽  
Active Matrix ◽  
Probing Pocket Depth ◽  
Whole Saliva ◽  
Biomarker Testing ◽  
Crevicular Fluid

Abstract Background Pathologically elevated levels of matrix metalloproteinase-8 (MMP-8) and Lactoferrin in oral fluids have been associated with the presence of gingivitis/periodontitis. This study aimed to assess the origin of MMP-8 and Lactoferrin in periodontitis patients and to identify the degree to which conventional clinical parameters correlate with their presence. Methods A total of ten periodontitis and ten healthy patients were included in this study. Whole saliva (stimulated and unstimulated), parotid/sublingual glandular fluid and gingival crevicular fluid from pockets and sulci were tested for MMP-8 and Lactoferrin and protein concentrations were quantified using an ELISA assay. Clinical parameters were checked for potential associations with MMP-8 and Lactoferrin levels. Results Periodontal patients presented higher concentrations of MMP-8 and Lactoferrin in pockets than other sources (P = 0.03). Lactoferrin measurement was higher in the parotid compared to sublingual glandular fluid in periodontitis patients (P = 0.03). Increased probing pocket depth was positively correlated with high MMP-8 and Lactoferrin levels. Conclusions Periodontal pockets appear to be the major source of active matrix metalloproteinase and Lactoferrin, which also may also enter the oral cavity through the salivary glands. Since clinically healthy sites in periodontitis patients also had elevated biomarker levels, gingival crevicular fluid biomarker testing may be more predictive of future tissue breakdown than conventional clinical parameters.

scholarly journals Application of Vibrational Spectroscopies in the Qualitative Analysis of Gingival Crevicular Fluid and Periodontal Ligament during Orthodontic Tooth Movement

2021 ◽  
Vol 10 (7) ◽  
pp. 1405
Author(s):  
Fabrizia d’Apuzzo ◽  
Ludovica Nucci ◽  
Ines Delfino ◽  
Marianna Portaccio ◽  
Giuseppe Minervini ◽  
...  
Keyword(s):  
Periodontal Ligament ◽  
Tooth Movement ◽  
Gingival Crevicular Fluid ◽  
Orthodontic Tooth Movement ◽  
High Sensitivity ◽  
Data Analysis Procedure ◽  
Vibrational Spectroscopies ◽  
Composition Structure ◽  
The Individual ◽  
Crevicular Fluid

Optical vibrational techniques show a high potentiality in many biomedical fields for their characteristics of high sensitivity in revealing detailed information on composition, structure, and molecular interaction with reduced analysis time. In the last years, we have used these techniques for investigating gingival crevicular fluid (GCF) and periodontal ligament (PDL) during orthodontic tooth treatment. The analysis with Raman and infrared signals of GCF and PDL samples highlighted that different days of orthodontic force application causes modifications in the molecular secondary structure at specific wavenumbers related to the Amide I, Amide III, CH deformation, and CH3/CH2. In the present review, we report the most relevant results and a brief description of the experimental techniques and data analysis procedure in order to evidence that the vibrational spectroscopies could be a potential useful tool for an immediate monitoring of the individual patient’s response to the orthodontic tooth movement, aiming to more personalized treatment reducing any side effects.

scholarly journals Hydrogen peroxide-based products alter inflammatory and tissue damage-related proteins in the gingival crevicular fluid of healthy volunteers: a randomized trial

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Valderlane L. P. Colares ◽  
Suellen N. L. Lima ◽  
Nágila C. F. Sousa ◽  
Mizael C. Araújo ◽  
Domingos M. S. Pereira ◽  
...  
Keyword(s):  
Hydrogen Peroxide ◽  
Randomized Trial ◽  
Healthy Volunteers ◽  
Tissue Damage ◽  
Gingival Crevicular Fluid ◽  
Crevicular Fluid ◽  
Related Proteins

scholarly journals Effects of Initial Periodontal Therapy on Heat Shock Protein 70 Levels in Gingival Crevicular Fluid from Periodontitis Patients

2020 ◽  
Vol 9 (10) ◽  
pp. 3072
Author(s):  
Nobuhisa Furuse ◽  
Hideki Takai ◽  
Yorimasa Ogata
Keyword(s):  
Heat Shock ◽  
Gingival Crevicular Fluid ◽  
Periodontal Therapy ◽  
Enzyme Linked Immunosorbent Assay ◽  
Microbial Infection ◽  
Pocket Depth ◽  
Probing Pocket Depth ◽  
Factor Α ◽  
Crevicular Fluid ◽  
Shock Proteins

Periodontitis is an inflammatory disease of periodontium which is caused by periodontopathic bacteria. Moreover, various cytokines such as interleukin-1β (IL-1β), tumor necrosis factor-α (TNF-α), and IL-6 are expressed in the inflamed periodontium. Heat shock proteins (HSPs) protect cells from abnormal conditions including inflammation, microbial infection and diseases. The 70-kDa HSPs (HSP70s) are major HSPs that express in the inflamed tissues. In this study, an enzyme-linked immunosorbent assay was applied to measure the levels of HSP70 in gingival crevicular fluid (GCF) from two periodontal pockets in each of 10 patients with Stage III, Grade B periodontitis. Sites with probing pocket depth (PPD) of ≤3 mm were named the healthy control (HC) sites, and sites with PPD of ≥5 mm were named the diseased sites. HSP70 levels in GCF were expressed higher at diseased sites than at HC sites, and decreased after initial periodontal therapy at diseased sites. These results suggest the association of HSP70 with the stage of periodontitis.

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