scholarly journals Antioxidant potential, in vitro cytotoxicity and apoptotic effect induced by crude organic extract of Anthracophyllum lateritium against RD sarcoma cells

2015 ◽  
Vol 15 (1) ◽  
Author(s):  
Dilusha M Fernando ◽  
Ravi LC Wijesundera ◽  
Preethi Soysa ◽  
Dilip de Silva ◽  
Chandrika M Nanayakkara
Keyword(s):  
In Vitro Cytotoxicity ◽  
Antioxidant Potential ◽  
Organic Extract ◽  
Apoptotic Effect ◽  
Sarcoma Cells

HYALURONIC ACID-DOCETAXEL CONJUGATE LOADED NANOLIPOSOMES FOR TARGETING TUMOR CELLS

2020 ◽  
pp. 88-99
Author(s):  
MULUNEH FROMSA SEIFU ◽  
LILA KANTA NATH ◽  
DEBASHIS DUTTA
Keyword(s):  
Hyaluronic Acid ◽  
Glial Cells ◽  
Cellular Uptake ◽  
Drug Loading ◽  
Microscopic Investigation ◽  
In Vitro Cytotoxicity ◽  
Scanning Calorimetry ◽  
Anticancer Efficacy ◽  
Apoptotic Effect

Objective: Docetaxel (DTX), a potent anticancer drug, is suffering from non-specificity and drug resistance as major limitations. In this investigation, we developed Hyaluronic acid (HA)-Docetaxel conjugate (HA-DTX) loaded nanoliposomes to target cancer cells via passive and active targeting approaches. Methods: HA-DTX was synthesized and characterized by UV-Visible spectrophotometry, FT-IR spectroscopy, 1H NMR spectroscopy, Differential scanning calorimetry and X-ray diffraction and then loaded into nanoliposomes (L-NLs) by thin-film hydration method. L-NLs were characterized physicochemically and evaluated for anticancer efficacy by in vitro cytotoxicity study in glioma cells (C6 glial cells); cellular uptake and apoptotic effect were investigated by fluorescence microscopy. Results: HA-DTX was successfully synthesized; L-NLs had an average size of 123.0±16.53 nm, polydispersity index of 0.246±0.01 and zeta potential of 44.4±6.79 mV. Also, L-NLs exhibited 90.54%±4.22 of drug loading efficiency and 2.68%±0.12 of drug loading, releasing about 57.72%±1.17 at pH 5.2 and only 14.14%±1.32 at pH 7.4 after 48 h. No significant change instability was observed after storage at 5 °C±3 °C as well as at 25 °C±2 °C/60% RH±5% RH for 6 mo. The cytotoxicity effect of L-NLs was higher by 10% that of marketed formulation at 10 µg/ml docetaxel concentration. Fluorescence microscopic investigation showed that more cellular uptake and apoptotic effect were observed in L-NLs treated C6 glial cells than in those treated with the marketed formulation. Conclusion: HA-DTX loaded nanoliposomes enabled docetaxel to target C6 glial cells with better efficacy and might be effective to treat glioma.

scholarly journals ANTIOXIDANT POTENTIAL, ANTITUMOR ACTIVITY AND PHENOLIC PROFILE OF PHOENIX DACTYLIFERA LINN

2017 ◽  
Vol 9 (5) ◽  
pp. 130
Author(s):  
Manal Mortady Hamed ◽  
Mona Abdel Motagally Mohamed ◽  
Wafaa Sabry Ahmed
Keyword(s):  
Antitumor Activity ◽  
Phoenix Dactylifera ◽  
In Vitro Cytotoxicity ◽  
Antioxidant Potential ◽  
Butanol Extract ◽  
Chemical Structures ◽  
Total Antioxidant ◽  
Ic50 Values ◽  
Date Fruits

Objective: Phoenix dactylifera Linn (Fam.: arecaceae)or date fruits are commercial crops that notarized in holy quran. 70% aqueous MeOH extract of the fruits led to isolation of six compounds; its chemical structures were determined as, β-sitosterol (1), caffeic acid (2), ferulic acid (3), protocatechuic acid (4), p-hydroxybenzoic acid (5) and luteolin (6).Methods: The accurately weighed date fruits were washed, sliced and socked freshly in 70% methanol then exhaustively extracted under reflux for about 2 w and filtered, then fractionated by different solvent; finaly the butanol extract evaporated and fractionated on a polyamide glass column. Using Sephadex LH-20 column to purify the compounds obtained. In our preliminary study, the extracts and compounds were subjected to in vitro cytotoxicity against HepG2 cell line through the MTT assay and the antioxidant potential of the extracts and pure compounds were assayed through in vitro model using (DPPH) and phosphomolybdenum assays.Results: Compounds 2 and 3 exhibited promising antitumor activity with IC50 values of 6 and 10 μg/ml, respectively. Moreover compounds 1, 4, 5 and 6 showed cytotoxic activity with IC50 values of 13, 15, 21 and 35 μg/ml, respectively. The antioxidant potential of the compounds showed the inhibition percentage values (SC50) ranged from 4.36 to 10.25 μg/ml, while the total antioxidant capacity ranged from (583.66 to 702.00 mg AAE/g compound).Conclusion: Our study demonstrated that; dates constituents are powerful as antioxidant and antitumor; hence it is the best potential for pharmaceutical applications.

In vitro Cytotoxicity of Bisphenol A in Monocytes Cell Line

2015 ◽  
Vol 40 (2) ◽  
pp. 180-186 ◽  
Author(s):  
Mauro Neri ◽  
Grazia Maria Virzì ◽  
Alessandra Brocca ◽  
Francesco Garzotto ◽  
Jeong Chul Kim ◽  
...  
Keyword(s):  
Bisphenol A ◽  
Cell Line ◽  
Direct Contact ◽  
Cytotoxic Effect ◽  
In Vitro Cytotoxicity ◽  
Maximum Value ◽  
In Vitro Investigation ◽  
Apoptotic Effect ◽  
Monocyte Cell

Background/Aim: Bisphenol A (BPA) is used in the production of many plastics, which are used to build biomaterials that sometimes are in direct contact with blood. It is believed that the release of BPA into bloodstream may give rise to cytotoxic events for blood components. The aim of the present study was to perform an in vitro investigation of the observable cytotoxic effect of BPA, at increasing concentrations, on the monocyte cell line. Methods: We incubated in vitro monocyte cells (U937) for 24 h in cell line medium samples (RPMI 1640) at different concentrations of BPA. We then generated curves to evaluate viability, necrosis and apoptosis of monocytes against increasing concentrations of BPA. Results: The percentage values of concentrations of BPA corresponding to 50% of the viability and necrosis of the monocytes were 1.39 and 1.48 ng/ml, respectively. Based on our observations, we reported an increasing cytotoxic effect for higher concentrations. The apoptotic effect reached the maximum value at BPA concentration of 1.5 ng/ml; at still higher concentrations, we observed a predominantly necrotic cell death. Conclusion: Viability, necrosis and apoptosis of monocytes are strongly and positively correlated with BPA concentration. A direct contact of such compound with biological components of blood may lead to high levels of cytotoxicity, and require us to evaluate additional factors while judging the bio-incompatibility of BPA.

scholarly journals Leaf Anatomical-Histophytochemical Study and Evaluation of the Cytotoxicity of Ottonia frutescens Trel (Piperaceae)

2020 ◽  
Vol 11 (2) ◽  
pp. 8760-8772
Keyword(s):  
Leaf Extract ◽  
Antioxidant Potential ◽  
Leukemic Cells ◽  
Human Leukemia ◽  
U937 Cells ◽  
Leaf Extracts ◽  
Apoptotic Effect ◽  
First Time ◽  
Dose Dependent

To investigate the leaf histochemistry and micromorphology of Ottonia frutescens Schltdl Trel (Piperaceae), the antioxidant potential, and in vitro cytotoxic bioactivity of the leaf extract of this species against human leukemia and colorectal cells. Leaf micromorphology and histoanatomy were detailed and recorded by optical and transmission and scanning electron microscopy. The histochemical results reveal classes of secondary phytometabolites such as alkaloids, anthraquinones, coumarins, polyphenols, and saponins. Cardiotonics, flavonoids, and triterpenes compounds were not detected. All concentrations of leaf extracts showed a significant dose-dependent and time-dependent apoptotic effect with more than 90% apoptosis of U937 cells and about 60% apoptosis of COLO-205 cells in 24 h of culture under 200 μg/mL of leaf extract. In addition, the phenolic compounds present in the fractions of the sample contribute to its strong antioxidant capacity. Ottonia frutescens exhibit anatomical and histochemical characteristics similar to other Piperaceae, and for the first time, provides a new source for secondary metabolite classes that exhibit high antioxidant potential and cytotoxic bioactivity, reduce cell viability and induce apoptosis in U937 leukemic cells and COLO-205 colorectal cells.

scholarly journals Antimicrobial and Antioxidant Potential of Scenedesmus obliquus Microalgae in the Context of Integral Biorefinery Concept

Molecules ◽  
2022 ◽  
Vol 27 (2) ◽  
pp. 519
Author(s):  
Maya Margaritova Zaharieva ◽  
Dimitrina Zheleva-Dimitrova ◽  
Snezhana Rusinova-Videva ◽  
Yana Ilieva ◽  
Anna Brachkova ◽  
...  
Keyword(s):  
Scenedesmus Obliquus ◽  
Red Light ◽  
Green Light ◽  
Initial Step ◽  
In Vitro Cytotoxicity ◽  
Antioxidant Potential ◽  
Small Scale ◽  
Microalgal Biomass ◽  
Oregano Essential Oil

Small-scale photobioreactors (PBRs) in the inoculum stage were designed with internal (red or green) and external white LED light as an initial step of a larger-scale installation aimed at fulfilling the integral biorefinery concept for maximum utilization of microalgal biomass in a multifunctional laboratory. The specific growth rate of Scenedesmus obliquus (Turpin) Kützing biomass for given cultural conditions was analyzed by using MAPLE software. For the determination of total polyphenols, flavonoids, chlorophyll “a” and “b”, carotenoids and lipids, UHPLC-HRMS, ISO-20776/1, ISO-10993-5 and CUPRAC tests were carried out. Under red light growing, a higher content of polyphenols was found, while the green light favoured the flavonoid accumulation in the biomass. Chlorophylls, carotenoids and lipids were in the same order of magnitude in both samples. The dichloromethane extracts obtained from the biomass of each PBR synergistically potentiated at low concentrations (0.01–0.05 mg/mL) the antibacterial activity of penicillin, fluoroquinolones or oregano essential oil against the selected food-borne pathogens (Staphylococcus aureus, Escherichia coli and Salmonella typhimurium) without showing any in vitro cytotoxicity. Both extracts exhibited good cupric ion-reducing antioxidant capacity at concentrations above 0.042–0.08 mg/mL. The UHPLC-HRMS analysis revealed that both extracts contained long chain fatty acids and carotenoids thus explaining their antibacterial and antioxidant potential. The applied engineering approach showed a great potential to modify microalgae metabolism for the synthesis of target compounds by S. obliquus with capacity for the development of health-promoting nutraceuticals for poultry farming.

Screening of in vitro cytotoxicity, antioxidant potential and bioactivity of nano- and micro-ZrO2 and -TiO2 particles

2013 ◽  
Vol 93 ◽  
pp. 191-197 ◽  
Author(s):  
Gopalu Karunakaran ◽  
Rangaraj Suriyaprabha ◽  
Palanisamy Manivasakan ◽  
Rathinam Yuvakkumar ◽  
Venkatachalam Rajendran ◽  
...  
Keyword(s):  
In Vitro Cytotoxicity ◽  
Antioxidant Potential ◽  
Tio2 Particles

scholarly journals IN VITRO CYTOTOXICITY EFFECT ON MCF-7 CELL LINE OF CO-ENCAPSULATED ARTESUNATE AND CURCUMIN LIPOSOME

2017 ◽  
Vol 9 (3) ◽  
pp. 123 ◽  
Author(s):  
Sarvesh Sharma ◽  
Vimal Kumar
Keyword(s):  
Drug Loading ◽  
In Vitro Cytotoxicity ◽  
Cancer Drug ◽  
Physiochemical Parameters ◽  
Apoptotic Effect ◽  
Ameliorative Effect ◽  
Indian Food ◽  
Photo Stability ◽  
Mcf 7

Objective: Cancer drug delivery has focused using novel carrier system due to their high drug loading and release controlled property and minimum side effect. Artesunate (ART) and curcumin (CUR) are proved natural herbal lead chemotherapeutics used as Chinese and Indian food. But due to its low absorption and the poor bioavailability limits its clinical efficacy. The object of the present work was to investigate the cytotoxic vs apoptotic effect of these herbal lead molecules in the co-encapsulated liposome formulation.Methods: ART and CUR liposome were prepared by thin film hydration method. Because of their opposite solubility nature, ART loaded in the aqueous phase at pH 7.4 while CUR is present in the central core through lipophilic interaction. In vitro cytotoxicity action of the free and liposomal formulation was performed by MTT assay.Results: Physiochemical parameters were evaluated and compared to the co-encapsulated formulation of containing both molecules. The mean diameter around 200 nm, low polydispersity index and co-encapsulation efficiency were found to be 90%. Co-encapsulation in nanometer size is beneficial for uptake and photo-stability of a drug. The in vitro cytotoxic effect of the co-encapsulated formulation was shown a better result than individual drug and also gives the clue for apoptosis. IC50 value for ART, CUR and Liposome was found to be 297.61 µg/ml and 60.60 µg/ml respectively. The result explained the co-encapsulation of curcumin with artesunate is show an ameliorative effect for repositioning therapeutic efficacy of the drug.Conclusion: We observed that human breast cancer MCF-7 cells were relatively resistant to ART and sensitive to CUR. Treatment with ART plus CUR had a synergistic cytotoxic, and apoptotic effect was mediated by up regulation of DR4 and DR5 m RNA expression.

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