scholarly journals Evolution of the codling moth pheromone via an ancient gene duplication

BMC Biology ◽  
2021 ◽  
Vol 19 (1) ◽  
Author(s):  
Jean-Marc Lassance ◽  
Bao-Jian Ding ◽  
Christer Löfstedt
Keyword(s):  
Gene Family ◽  
Developmental Stages ◽  
Cydia Pomonella ◽  
Fatty Acid Desaturase ◽  
Codling Moth ◽  
Fatty Acyl ◽  
Integrative Approach ◽  
Heterologous Expression Systems ◽  
Genetic Novelty ◽  
Ancient Gene

AbstractBackgroundDefining the origin of genetic novelty is central to our understanding of the evolution of novel traits. Diversification among fatty acid desaturase (FAD) genes has played a fundamental role in the introduction of structural variation in fatty acyl derivatives. Because of its central role in generating diversity in insect semiochemicals, the FAD gene family has become a model to study how gene family expansions can contribute to the evolution of lineage-specific innovations. Here we used the codling moth (Cydia pomonella) as a study system to decipher the proximate mechanism underlying the production of the ∆8∆10 signature structure of olethreutine moths. Biosynthesis of the codling moth sex pheromone, (E8,E10)-dodecadienol (codlemone), involves two consecutive desaturation steps, the first of which is unusual in that it generates anE9 unsaturation. The second step is also atypical: it generates a conjugated diene system from theE9 monoene C12intermediate via 1,4-desaturation.ResultsHere we describe the characterization of the FAD gene acting in codlemone biosynthesis. We identify 27 FAD genes corresponding to the various functional classes identified in insects and Lepidoptera. These genes are distributed across theC. pomonellagenome in tandem arrays or isolated genes, indicating that the FAD repertoire consists of both ancient and recent duplications and expansions. Using transcriptomics, we show large divergence in expression domains: some genes appear ubiquitously expressed across tissue and developmental stages; others appear more restricted in their expression pattern. Functional assays using heterologous expression systems reveal that one gene, Cpo_CPRQ, which is prominently and exclusively expressed in the female pheromone gland, encodes an FAD that possesses bothE9 and ∆8∆10 desaturation activities. Phylogenetically, Cpo_CPRQ clusters within the Lepidoptera-specific ∆10/∆11 clade of FADs, a classic reservoir of unusual desaturase activities in moths.ConclusionsOur integrative approach shows that the evolution of the signature pheromone structure of olethreutine moths relied on a gene belonging to an ancient gene expansion. Members of other expanded FAD subfamilies do not appear to play a role in chemical communication. This advises for caution when postulating the consequences of lineage-specific expansions based on genomics alone.

scholarly journals Evolution of the Codling Moth Pheromone Through the Member of an Ancient Desaturase Expansion

2020 ◽  
Author(s):  
Jean-Marc Lassance ◽  
Bao-Jian Ding ◽  
Christer Löfstedt
Keyword(s):  
Gene Family ◽  
Developmental Stages ◽  
Cydia Pomonella ◽  
Fatty Acid Desaturase ◽  
Codling Moth ◽  
Fatty Acyl ◽  
Integrative Approach ◽  
Functional Classes ◽  
Heterologous Expression Systems ◽  
Genetic Novelty

AbstractDefining the origin of genetic novelty is central to our understanding of the evolution of novel traits. Diversification among fatty acid desaturase (FAD) genes has played a fundamental role in the introduction of structural variation in fatty acyl derivatives. Because of its central role in generating diversity in insect semiochemicals, the FAD gene family has become a model to study how gene family expansions can contribute to the evolution of lineage-specific innovations. Here we used the codling moth (Cydia pomonella) as a study system to decipher the proximate mechanism underlying the production of the Δ8Δ10 signature structure of Olethreutine moths. Biosynthesis of the codling moth sex pheromone, (E8,E10)-dodecadienol (codlemone), involves two consecutive desaturation steps, the first of which is unusual in that it generates an E9 unsaturation. The second step is also atypical: it generates a conjugated diene system from the E9 monoene C12 intermediate via 1,4-desaturation. Here we describe the characterization of the FAD gene acting in codlemone biosynthesis. We identify 27 FAD genes corresponding to the various functional classes identified in Insects and Lepidoptera. These genes are distributed across the C. pomonella genome in tandem arrays or isolated genes, indicating that the FAD repertoire consists of both ancient and recent duplications and expansions. Using transcriptomics, we show large divergence in expression domains: some genes appear ubiquitously expressed across tissue and developmental stages; others appear more restricted in their expression pattern. Functional assays using heterologous expression systems reveal that one gene, Cpo_CPRQ, which is prominently and exclusively expressed in the female pheromone gland, encodes an FAD that possesses both E9 and Δ8Δ10-desaturation activities. Phylogenetically, Cpo_CPRQ clusters within the Lepidoptera-specific Δ10/Δ11 clade of FADs, a classic reservoir of unusual desaturase activities in moths. Our integrative approach shows that the evolution of the signature pheromone structure of Olethreutine moths relied on a gene belonging to an ancient gene expansion. Members of other expanded FAD subfamilies do not appear to play a role in chemical communication. This advises for caution when postulating the consequences of lineage-specific expansions based on genomics alone.

Biology of the codling Moth Cydia (Carpocapsa = Laspeyresia) Pomonella L. (Olethreutidae, Lepidoptera) and effect of fruit age on its diapause induction

1978 ◽  
Vol 90 (1) ◽  
pp. 233-236 ◽  
Author(s):  
F. M. El-Gamil ◽  
I. A. Gaaboub ◽  
S. K. El-Sawaf
Keyword(s):  
Developmental Stages ◽  
Cydia Pomonella ◽  
Codling Moth ◽  
Diapause Induction ◽  
Larval Food ◽  
Laboratory Conditions ◽  
Maturity Stages ◽  
Laspeyresia Pomonella ◽  
June July ◽  
New Generation

SUMMARYThe number of generations and the durations of the developmental stages of the codling moth (Cydia pomonella L.) that fed on fresh pear fruits were studied during a whole year, 1973, under laboratory conditions. In the next year, diapause of larvae was investigated in relation to the larval food.Results showed that this insect reproduced four generations on pear fruits under laboratory conditions and diapaused during the whole period of the autumn. Broods of these generations occurred during April, June, July and September, respectively.There was a suggestion that the larval food affected the induction of diapause in this insect. About 83% of larvae which fed on ‘ripe native Volos apples’ diapaused as early as the second half of June although the majority of larvae (93%) which fed on ‘immature pears’ continued development to adults and only 7% began to diapause under the same conditions. Up to 98% of larvae which fed on ‘fully mature ripe pears’ did not develop beyond the cocooning stage until the following spring, but 88% of larvae fed on immature pears stored at 4 °C for about 3 months reproduced a new generation about the end of October. These variable results should be attributed to the nourishment on different host-fruits in different maturity stages.

scholarly journals Green Chemistry Production of Codlemone, the Sex Pheromone of the Codling Moth (Cydia pomonella), by Metabolic Engineering of the Oilseed Crop Camelina (Camelina sativa)

2021 ◽  
Author(s):  
Yi-Han Xia ◽  
Hong-Lei Wang ◽  
Bao-Jian Ding ◽  
Glenn P. Svensson ◽  
Carin Jarl-Sunesson ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Sex Pheromone ◽  
Cydia Pomonella ◽  
Codling Moth ◽  
Camelina Sativa ◽  
Fatty Acyl ◽  
Flight Tunnel ◽  
Oilseed Crop ◽  
Umbellularia Californica ◽  
Total Fatty Acids

AbstractSynthetic pheromones have been used for pest control over several decades. The conventional synthesis of di-unsaturated pheromone compounds is usually complex and costly. Camelina (Camelina sativa) has emerged as an ideal, non-food biotech oilseed platform for production of oils with modified fatty acid compositions. We used Camelina as a plant factory to produce mono- and di-unsaturated C12 chain length moth sex pheromone precursors, (E)-9-dodecenoic acid and (E,E)-8,10-dodecadienoic acid, by introducing a fatty acyl-ACP thioesterase FatB gene UcTE from California bay laurel (Umbellularia californica) and a bifunctional ∆9 desaturase gene Cpo_CPRQ from the codling moth, Cydia pomonella. Different transgene combinations were investigated for increasing pheromone precursor yield. The most productive Camelina line was engineered with a vector that contained one copy of UcTE and the viral suppressor protein encoding P19 transgenes and three copies of Cpo_CPRQ transgene. The T2 generation of this line produced 9.4% of (E)-9-dodecenoic acid and 5.5% of (E,E)-8,10-dodecadienoic acid of the total fatty acids, and seeds were selected to advance top-performing lines to homozygosity. In the T4 generation, production levels of (E)-9-dodecenoic acid and (E,E)-8,10-dodecadienoic acid remained stable. The diene acid together with other seed fatty acids were converted into corresponding alcohols, and the bioactivity of the plant-derived codlemone was confirmed by GC-EAD and a flight tunnel assay. Trapping in orchards and home gardens confirmed significant and specific attraction of C. pomonella males to the plant-derived codlemone.

The results of field trials of the codling moth pheromone preparations in 2020

2020 ◽  
Author(s):  
S.V. Dmitriyeva ◽  
◽  
I.M. Mityushev
Keyword(s):  
Russian Federation ◽  
Central Region ◽  
Cydia Pomonella ◽  
Codling Moth ◽  
Field Trials ◽  
Field Screening ◽  
Tube Type ◽  
The Russian Federation ◽  
Polyethylene Dispenser

This article presents the results of field screening of pheromone preparations of the codling moth, Cydia pomonella L., conducted in 2020 under conditions of the Central Region of the Russian Federation. The new «Tube» type dispensers were tested vs. standard foil-polyethylene dispenser.

scholarly journals Genome wide analysis of kinesin gene family in Citrullus lanatus reveals an essential role in early fruit development

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Shujuan Tian ◽  
Jiao Jiang ◽  
Guo-qi Xu ◽  
Tan Wang ◽  
Qiyan Liu ◽  
...  
Keyword(s):  
Gene Family ◽  
Plant Hormones ◽  
Fruit Development ◽  
Developmental Stages ◽  
Profile Analysis ◽  
Citrullus Lanatus ◽  
Binding Motif ◽  
Distribution Analysis ◽  
Motif Analysis ◽  
Expression Profile Analysis

Abstract Background Kinesin (KIN) as a motor protein is a versatile nano-machine and involved in diverse essential processes in plant growth and development. However, the kinesin gene family has not been identified in watermelon, a valued and nutritious fruit, and yet their functions have not been characterized. Especially, their involvement in early fruit development, which directly determines the size, shape, yield and quality of the watermelon fruit, remains unclear. Results In this study, we performed a whole-genome investigation and comprehensive analysis of kinesin genes in C. lanatus. In total, 48 kinesins were identified and categorized into 10 kinesin subfamilies groups based on phylogenetic analysis. Their uneven distribution on 11 chromosomes was revealed by distribution analysis. Conserved motif analysis showed that the ATP-binding motif of kinesins was conserved within all subfamilies, but not the microtubule-binding motif. 10 segmental duplication pairs genes were detected by the syntenic and phylogenetic approaches, which showed the expansion of the kinesin gene family in C. lanatus genome during evolution. Moreover, 5 ClKINs genes are specifically and abundantly expressed in early fruit developmental stages according to comprehensive expression profile analysis, implying their critical regulatory roles during early fruit development. Our data also demonstrated that the majority of kinesin genes were responsive to plant hormones, revealing their potential involvement in the signaling pathways of plant hormones. Conclusions Kinesin gene family in watermelon was comprehensively analyzed in this study, which establishes a foundation for further functional investigation of C. lanatus kinesin genes and provides novel insights into their biological functions. In addition, these results also provide useful information for understanding the relationship between plant hormone and kinesin genes in C. lanatus.

scholarly journals Genome-Wide Analysis and Characterization of the Aux/IAA Family Genes Related to Floral Scent Formation in Hedychium coronarium

2019 ◽  
Vol 20 (13) ◽  
pp. 3235 ◽  
Author(s):  
Yanguo Ke ◽  
Farhat Abbas ◽  
Yiwei Zhou ◽  
Rangcai Yu ◽  
Yuechong Yue ◽  
...  
Keyword(s):  
Gene Family ◽  
Developmental Stages ◽  
Floral Scent ◽  
Expression Profiles ◽  
Expression Patterns ◽  
Regulatory Elements ◽  
Virus Induced Gene Silencing ◽  
Cut Flowers ◽  
Flower Opening ◽  
Hedychium Coronarium

Auxin plays a key role in different plant growth and development processes, including flower opening and development. The perception and signaling of auxin depend on the cooperative action of various components, among which auxin/indole-3-acetic acid (Aux/IAA) proteins play an imperative role. In a recent study, the entire Aux/IAA gene family was identified and comprehensively analyzed in Hedychium coronarium, a scented species used as an ornamental plant for cut flowers. Phylogenetic analysis showed that the Aux/IAA gene family in H. coronarium is slightly contracted compared to Arabidopsis, with low levels of non-canonical proteins. Sequence analysis of promoters showed numerous cis-regulatory elements related to various phytohormones. HcIAA genes showed distinct expression patterns in different tissues and flower developmental stages, and some HcIAA genes showed significant responses to auxin and ethylene, indicating that Aux/IAAs may play an important role in linking hormone signaling pathways. Based on the expression profiles, HcIAA2, HcIAA4, HcIAA6 and HcIAA12, were selected as candidate genes and HcIAA2 and HcIAA4 were screened for further characterization. Downregulation of HcIAA2 and HcIAA4 by virus-induced gene silencing in H. coronarium flowers modified the total volatile compound content, suggesting that HcIAA2 and HcIAA4 play important roles in H. coronarium floral scent formation. The results presented here will provide insights into the putative roles of HcIAA genes and will assist the elucidation of their precise roles during floral scent formation.

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