scholarly journals Molecular detection and genetic characterization of Wenzhou virus in rodents in Guangzhou, China

2021 ◽  
Vol 17 (1) ◽  
Author(s):  
Nina Wang ◽  
Lichao Yang ◽  
Guohui Li ◽  
Xu Zhang ◽  
Jianwei Shao ◽  
...  
Keyword(s):  
Rattus Norvegicus ◽  
Respiratory Symptoms ◽  
Molecular Detection ◽  
Genetic Characterization ◽  
Suncus Murinus ◽  
Rt Pcr ◽  
High Genetic Diversity ◽  
Emerging Virus ◽  
Sequence Identities

Abstract Background Wenzhou virus (WENV), a newly discovered mammarenavirus in rodents, is associated with fever and respiratory symptoms in humans. This study was aimed to detect and characterize the emerging virus in rodents in Guangzhou, China. Results A total of 100 small mammals, including 70 Rattus norvegicus, 22 Suncus murinus, 4 Bandicota indica, 3 Rattus flavipectus, and 1 Rattus losea, were captured in Guangzhou, and their brain tissues were collected and pooled for metagenomic analysis, which generated several contigs targeting the genome of WENV. Two R. norvegicus (2.9%) were further confirmed to be infected with WENV by RT-PCR. The complete genome (RnGZ37-2018 and RnGZ40-2018) shared 85.1–88.9% nt and 83.2–96.3% aa sequence identities to the Cambodian strains that have been shown to be associated with human disease. Phylogenetic analysis showed that all identified WENV could be grouped into four different lineages, and the two Guangzhou strains formed an independent clade. We also analyzed the potential recombinant events occurring in WENV strains. Conclusions Our study showed a high genetic diversity of WENV strains in China, emphasizing the relevance of surveillance of this emerging mammarenavirus in both natural reservoirs and humans.

scholarly journals Distribution and genetic diversity of Enterovirus G (EV-G) on pig farms in Thailand

2021 ◽  
Vol 17 (1) ◽  
Author(s):  
Taveesak Janetanakit ◽  
Supassama Chaiyawong ◽  
Kamonpan Charoenkul ◽  
Ratanaporn Tangwangvivat ◽  
Ekkapat Chamsai ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Genetic Characterization ◽  
Rt Pcr ◽  
Cross Sectional Survey ◽  
High Genetic Diversity ◽  
Cross Sectional ◽  
Predominant Genotype ◽  
Diarrhea Virus ◽  
Pig Farms

Abstract Background Enterovirus G (EV-G) causes subclinical infections and is occasionally associated with diarrhea in pigs. In this study, we conducted a cross-sectional survey of EV-G in pigs from 73 pig farms in 20 provinces of Thailand from December 2014 to January 2018. Results Our results showed a high occurrence of EV-Gs which 71.6 % of fecal and intestinal samples (556/777) and 71.2 % of pig farms (52/73) were positive for EV-G by RT-PCR specific to the 5’UTR. EV-Gs could be detected in all age pig groups, and the percentage positivity was highest in the fattening group (89.7 %), followed by the nursery group (89.4 %). To characterize the viruses, 34 EV-G representatives were characterized by VP1 gene sequencing. Pairwise sequence comparison and phylogenetic analysis showed that Thai-EV-Gs belonged to the EV-G1, EV-G3, EV-G4, EV-G8, EV-G9 and EV-G10 genotypes, among which the EV-G3 was the predominant genotype in Thailand. Co-infection with different EV-G genotypes or with EV-Gs and porcine epidemic diarrhea virus (PEDV) or porcine deltacoronavirus (PDCoV) on the same pig farms was observed. Conclusions Our results confirmed that EV-G infection is endemic in Thailand, with a high genetic diversity of different genotypes. This study constitutes the first report of the genetic characterization of EV-GS in pigs in Thailand.

scholarly journals Molecular Detection and Characterization of Porcine Astrovirus on the basis of Partial ORF1b/ORF2 Region

2021 ◽  
Author(s):  
Rajpreet Kaur ◽  
PARVEEN KUMAR ◽  
Naresh Jindal ◽  
Sanjeevna Kumari Minhas ◽  
Anand Prakash ◽  
...  
Keyword(s):  
Molecular Detection ◽  
Rt Pcr ◽  
High Genetic Diversity ◽  
Pcr Screening ◽  
Porcine Astrovirus ◽  
Wide Range ◽  
Suckling Piglets ◽  
Weaning Piglets ◽  
Orf2 Region

Abstract The porcine astrovirus (PAstV) is distributed globally and exists as five distinct lineages (PAstV1-PAstV5). PAstV is considered one of the important pathogen associated with diarrhea among pigs. In the present study, the PAstV was detected in 13.4% (19/141) of fecal samples including 14.4% (16/111) diarrheic and 10% (3/30) non-diarrheic samples by RT-PCR based on partial ORF1b/ORF2 gene from Haryana, India. The results indicated that the weaning piglets were more susceptible to PAstV infection followed by suckling piglets. The phylogenetic analysis of the viral strains revealed the circulation ofPAstV4 (55.5%) and PAstV2 (44.4%) lineages with PAstV4 being the predominant lineage. To conclude, RT-PCR screening followed by sequencing of PAstV revealed high genetic diversity among the PAstV strains suggesting the wide range of heterogeneity and possible recombination events of viral strains in the state.

scholarly journals Subtype Characterization and Zoonotic Potential of Cryptosporidium felis in Cats in Guangdong and Shanghai, China

Pathogens ◽  
2021 ◽  
Vol 10 (2) ◽  
pp. 89
Author(s):  
Jiayu Li ◽  
Fuxian Yang ◽  
Ruobing Liang ◽  
Sheng Guo ◽  
Yaqiong Guo ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Phylogenetic Analysis ◽  
Genetic Characterization ◽  
Zoonotic Potential ◽  
Common Occurrence ◽  
High Genetic Diversity ◽  
Gp60 Gene ◽  
The Common ◽  
Subtyping Tool

Cryptosporidiumfelis is an important cause of feline and human cryptosporidiosis. However, the transmission of this pathogen between humans and cats remains controversial, partially due to a lack of genetic characterization of isolates from cats. The present study was conducted to examine the genetic diversity of C. felis in cats in China and to assess their potential zoonotic transmission. A newly developed subtyping tool based on a sequence analysis of the 60-kDa glycoprotein (gp60) gene was employed to identify the subtypes of 30 cat-derived C. felis isolates from Guangdong and Shanghai. Altogether, 20 C. felis isolates were successfully subtyped. The results of the sequence alignment showed a high genetic diversity, with 13 novel subtypes and 2 known subtypes of the XIXa subtype family being identified. The known subtypes were previously detected in humans, while some of the subtypes formed well-supported subclusters with human-derived subtypes from other countries in a phylogenetic analysis of the gp60 sequences. The results of this study confirmed the high genetic diversity of the XIXa subtype family of C. felis. The common occurrence of this subtype family in both humans and cats suggests that there could be cross-species transmission of C. felis.

Molecular detection and genetic characterization of ovine gammaherpesvirus-2 (OvHV-2) in sheep in the Philippines

2021 ◽  
Vol 199 ◽  
pp. 106383
Author(s):  
Innina R. Mananguit ◽  
Nest Dale F. Bartolome ◽  
Gabriel Alexis S.P. Tubalinal ◽  
Claro N. Mingala
Keyword(s):  
Molecular Detection ◽  
Genetic Characterization ◽  
The Philippines

scholarly journals First molecular detection and genetic characterization of Coxiella burnetii in Zambian dogs and rodents

2018 ◽  
Vol 11 (1) ◽  
Author(s):  
Simbarashe Chitanga ◽  
Edgar Simulundu ◽  
Martin C. Simuunza ◽  
Katendi Changula ◽  
Yongjin Qiu ◽  
...  
Keyword(s):  
Coxiella Burnetii ◽  
Molecular Detection ◽  
Genetic Characterization

scholarly journals Prevalence and Genetic Characterization of Two Mitochondrial Gene Sequences of Strobilocercus Fasciolaris in the Livers of Brown Rats (Rattus norvegicus) in Heilongjiang Province in Northeastern China

2020 ◽  
Vol 10 ◽  
Author(s):  
Fengnian Zhao ◽  
Yun Zhou ◽  
Yanchen Wu ◽  
Kexin Zhou ◽  
Aiqin Liu ◽  
...  
Keyword(s):  
Amino Acid ◽  
Intraspecific Variation ◽  
Rattus Norvegicus ◽  
Genetic Characterization ◽  
Northeastern China ◽  
Amino Acid Sequences ◽  
Morphological Observation ◽  
Heilongjiang Province ◽  
Gene Sequences

Rodents constitute the largest and most successful group of mammals worldwide. Brown rats (Rattus norvegicus) are one of the most common rodent species, and they serve as intermediate hosts of Hydatigera taeniaeformis. Although there have been a few studies reporting on the presence of the larval form of H. taeniaeformis (strobilocercus fasciolaris) in brown rats worldwide, little information is available on the genetic characterization of this parasite, with no molecular data from China. Therefore, from April 2014 to March 2016, this study was carried out to understand the prevalence and genetic characters of strobilocercus fasciolaris in brown rats captured in Heilongjiang Province in northeastern China. The livers of brown rats were collected and examined for the presence of cysts. Each cyst was identified based on morphological observation: the larvae with the naked eye and the scolexes under a microscope. The results were confirmed by polymerase chain reaction (PCR) and sequencing of the cytochrome c oxidase subunit 1 (cox1) and NADH dehydrogenase subunit 4 (nad4) genes. At the investigated sites, 11.8% (13/110) of the brown rats were infected with strobilocercus fasciolaris. Based on sequence analysis, there were 10 and six haplotypes regarding the cox1 and the nad4 loci, with 24 and 42 polymorphic sites, respectively (degree of intraspecific variation: 0.3%–4.4% and 0.6%–4.7%, respectively). Twelve nucleotide sequences (six of the 10 at the cox1 locus and all six at the nad4 locus) have not previously been described. Base differences in three of the six novel cox1 gene sequences and five of the six novel nad4 gene sequences caused amino acid changes. Phylogenetic analyses of the cox1 and nad4 gene sequences based on neighbor-joining and Bayesian inference trees indicated that all the strobilocercus fasciolaris isolates belonged to Hydatigera taeniaeformis sensu stricto (s.s.). This is the first report on the genetic characterization of strobilocercus fasciolaris in brown rats in China. The findings of novel cox1 and nad4 nucleotide and amino acid sequences may reflect the region-specific genetic characterization of the parasite. The data will be useful to explore the biological and epidemiological significance of the intraspecific variation within H. taeniaeformis s.s.

Moving towards an integrated approach to molecular detection and identification ofToxoplasma gondii

Parasitology ◽  
2009 ◽  
Vol 137 (1) ◽  
pp. 1-11 ◽  
Author(s):  
C. SU ◽  
E. K. SHWAB ◽  
P. ZHOU ◽  
X. Q. ZHU ◽  
J. P. DUBEY
Keyword(s):  
Molecular Detection ◽  
Genetic Characterization ◽  
Integrated Approach ◽  
Epidemiological Studies ◽  
Molecular Methods ◽  
Maximum Information ◽  
The Past ◽  
Phylogenetic Studies ◽  
Detection And Identification

SUMMARYThe development of simple, sensitive and rapid methods for the detection and identification ofToxoplasma gondiiis important for the diagnosis and epidemiological studies of the zoonotic disease toxoplasmosis. In the past 2 decades, molecular methods based on a variety of genetic markers have been developed, each with its advantages and limitations. The application of these methods has generated invaluable information to enhance our understanding of the epidemiology, population genetics and phylogeny ofT. gondii. However, since most studies focused solely on the detection but not genetic characterization ofT. gondii, the information obtained was limited. In this review, we discuss some widely used molecular methods and propose an integrated approach for the detection and identification ofT. gondii, in order to generate maximum information for epidemiological, population and phylogenetic studies of this key pathogen.

Sign in / Sign up

Export Citation Format

Share Document