scholarly journals Evaluation of the effect of vitamin D supplementation on spermatogram, seminal and serum levels of oxidative stress indices in asthenospermia infertile men: a study protocol for a triple-blind, randomized controlled trial

2021 ◽  
Vol 20 (1) ◽  
Author(s):  
Leila Maghsoumi-Norouzabad ◽  
Ahmad Zare Javid ◽  
Anahita Mansoori ◽  
Mohammadreza Dadfar ◽  
Amirarsalan Serajian
Keyword(s):  
Oxidative Stress ◽  
Vitamin D ◽  
Serum Levels ◽  
Vitamin D Supplementation ◽  
Male Reproduction ◽  
Sperm Parameters ◽  
Sperm Dna Fragmentation ◽  
Target Tissue ◽  
Infertile Men ◽  
Endocrine Factors

Abstract Background It is suggested that vitamin D may have a beneficial role in male reproduction. The male reproductive system is a target tissue for vitamin D. This study will aim to evaluate the effects of vitamin D supplementation on sperm parameters, seminal and serum levels of oxidative stress and serum endocrine factors in asthenospermia infertile men. Methods/design This randomized, triple-blind, placebo-controlled clinical trial will be conducted on 86 infertile men with idiopathic asthenozoospermia (the mobility of sperm < 40% and rapid progressive sperm motility < 32%), with serum levels of vitamin D less than 30 ng / ml in the “Infertility Clinic of Ahvaz Jahad daneshgahi”, Iran. Main outcomes measure (s) Demographic data, dietary intake, physical activity, sun exposure, anthropometric indices, serum and seminal levels of MDA (Malondialdehyde), 8-hydroxy-2- Dioxy Guanosine (8-OHDG), Total Antioxidant Capacity (TAC) and calcium, sperm DNA fragmentation index (DFI), serum 25-OHD, luteinizing hormone (LH), follicle-stimulating hormone (FSH), total testosterone (T), estradiol (E2), sex hormone-binding globulin (SHBG), free androgen index (FAI = T/SHBG. 100), T/LH and T/E2 ratios, prolactin (PRO), parathyroid hormone (PTH), osteocalcin (OCN), phosphorus and sperm parameters. Discussion The deficiency of vitamin D as an antioxidant is common all over the world. Numerous observational studies have shown a positive association between vitamin D levels and semen quality. However, few clinical studies have been conducted in this area. So considering with the high prevalence of this antioxidant deficiency specifically in infertile men, it seems that the supplementation of vitamin D in infertile men with insufficient levels or deficiency may improve the status of oxidative stress and thereby may affect sperm parameters and endocrine factors involved in male fertility. Trial registration Iran Clinical Trials Registry, ID: IRCT20151128025274N4, registered on 28 March 2018.

scholarly journals The effect of rhFSH on Sperm DNA Fragmentation and sperm parameters in Oligozoospermia Infertile Men

2020 ◽  
Vol 8 (2) ◽  
pp. 55-62
Author(s):  
Atefeh Verdi ◽  
◽  
Seyedeh Saeideh Sahraei ◽  
Elham Asa ◽  
Rahil Jannatifar ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Dna Fragmentation ◽  
Follicle Stimulating Hormone ◽  
Sperm Parameters ◽  
Sperm Dna Fragmentation ◽  
Infertile Men ◽  
Sperm Dna ◽  
Before And After ◽  
Infertile Patients ◽  
Stimulating Hormone

Background: One of the main causes of male infertility is the negative effects of oxidative stress. Follicle-stimulating hormone (FSH) plays an essential role in spermatogenesis, as well as in the maintenance of sperm DNA integrity. This study aimed to determine whether the recombinant human follicle-stimulating hormone (rhFSH) treatment of sperm parameters could positively affect sperm DNA and oxidative DNA fragmentation in oligozoospermia infertile men. Materials and Methods: This interventional study was carried out on a sample of 50 oligozoospermia infertile men. To this end, sperm DNA fragmentation and ROS as an oxidative stress marker were measured before and after treatment with the rhFSH sperm parameters. Results: The sperm parameters (concentration, mobility, and morphology) were significantly different in the oligozoospermia infertile patients before and after the rhFSH treatment (p<0.05). Moreover, sperm DNA fragmentation had a significant decrease in the patients after the FSH treatment (p<0.05). In addition, the ROS level in sperm, and the malondialdehyde level of seminal plasma significantly decreased after the treatment (P<0.05). Conclusion: The findings indicated that the rhFSH treatment significantly improved the sperm parameters. Further, the treatment led to a meaningful reduction of sperm DNA fragmentation and oxidative stress in the oligozoospermia infertile patients. Similarly, the malondialdehyde concentration markedly decreased in correlation with DNA fragmentation after the rhFSH treatment.

Vitamin D Supplementation and Serum Levels of Magne-sium and Selenium in Type 2 Diabetes Mellitus Patients: Gender Dimorphic Changes

2014 ◽  
Vol 84 (1-2) ◽  
pp. 27-34 ◽  
Author(s):  
Nasser M. Al-Daghri ◽  
Khalid M. Alkharfy ◽  
Nasiruddin Khan ◽  
Hanan A. Alfawaz ◽  
Abdulrahman S. Al-Ajlan ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Type 2 Diabetes ◽  
Vitamin D ◽  
Type 2 Diabetes Mellitus ◽  
Serum Levels ◽  
Vitamin D Supplementation ◽  
Serum Selenium ◽  
Dependent Manner

The aim of our study was to evaluate the effects of vitamin D supplementation on circulating levels of magnesium and selenium in patients with type 2 diabetes mellitus (T2DM). A total of 126 adult Saudi patients (55 men and 71 women, mean age 53.6 ± 10.7 years) with controlled T2DM were randomly recruited for the study. All subjects were given vitamin D3 tablets (2000 IU/day) for six months. Follow-up mean concentrations of serum 25-hydroxyvitamin D [25-(OH) vitamin D] significantly increased in both men (34.1 ± 12.4 to 57.8 ± 17.0 nmol/L) and women (35.7 ± 13.5 to 60.1 ± 18.5 nmol/L, p < 0.001), while levels of parathyroid hormone (PTH) decreased significantly in both men (1.6 ± 0.17 to 0.96 ± 0.10 pmol/L, p = 0.003) and women (1.6 ± 0.17 to 1.0 ± 0.14 pmol/L, p = 0.02). In addition, there was a significant increase in serum levels of selenium and magnesium in men and women (p-values < 0.001 and 0.04, respectively) after follow-up. In women, a significant correlation was observed between delta change (variables at six months-variable at baseline) of serum magnesium versus high-density lipoprotein (HDL)-cholesterol (r = 0.36, p = 0.006) and fasting glucose (r = - 0.33, p = 0.01). In men, there was a significant correlation between serum selenium and triglycerides (r = 0.32, p = 0.04). Vitamin D supplementation improves serum concentrations of magnesium and selenium in a gender-dependent manner, which in turn could affect several cardiometabolic parameters such as glucose and lipids.

Vitamin D supplementation improves fasting insulin levels and HDL cholesterol in infertile men

2021 ◽  
Author(s):  
Rune Holt ◽  
Jørgen Holm Pedersen ◽  
Elsa Dinsdale ◽  
Filip Krag Knop ◽  
Anders Juul ◽  
...  
Keyword(s):  
Vitamin D ◽  
Placebo Group ◽  
Serum Insulin ◽  
Hdl Cholesterol ◽  
Vitamin D Supplementation ◽  
Vitamin D Status ◽  
Fasting Serum ◽  
Cholesterol Levels ◽  
Infertile Men ◽  
Fasting Plasma

Abstract Context Vitamin D has been linked with glucose and lipid metabolism. Men with impaired gonadal function have a higher risk of metabolic syndrome and mortality, and vitamin D status may be a reversible modulator. Objective Determine the effect of daily vitamin D and calcium supplementation for 150 days on glucose and lipid homeostasis in infertile men. Design A single-center, double-blinded, randomized clinical trial (NCT01304927), 307 infertile men were randomized (1:1) to a single dose of 300,000 IU cholecalciferol followed by 1,400 IU cholecalciferol + 500 mg of calcium daily (n=151) or placebo (n=156) for 150 days. Reported metabolic parameters including fasting plasma glucose, HbA1c, fasting serum insulin, homeostatic model assessment of insulin resistance (HOMA-IR), fasting plasma cholesterols and triglyceride were secondary endpoints. The primary endpoint semen quality has previously been reported. Results Men receiving vitamin D supplementation improved their vitamin D status, while vitamin D status was aggravated in the placebo group characterized by higher serum parathyroid hormone (PTH). At end of trial, men receiving vitamin D supplementation had 13% lower fasting serum insulin concentrations compared with the placebo-treated group (65 vs. 74 pmol/L, P = 0.018) and 19% lower HOMA-IR (2.2 vs. 2.7, P = 0.025). Moreover, men in the vitamin D group had higher high-density lipoprotein (HDL) cholesterol levels (1.38 vs. 1.32 mmol/L, P = 0.008) compared with the placebo group. Conclusion High-dose vitamin D supplementation had beneficial effects on glucose homeostasis and HDL cholesterol levels in infertile men.

Association of Vitamin D Status with Semen Parameters and Male Reproductive Hormones in Egyptian Infertile Men

2021 ◽  
Vol 5 (1) ◽  
Author(s):  
Doaa Abou-Taleb AE ◽  
◽  
Mahran AM ◽  
Mahmoud MA ◽  
Gaber MA ◽  
...  
Keyword(s):  
Vitamin D ◽  
Significant Positive Correlation ◽  
Reproductive Hormones ◽  
Male Reproduction ◽  
Semen Parameters ◽  
Unknown Etiology ◽  
Control Group ◽  
Positive Correlation ◽  
Infertile Men

Background: Although vitamin D receptor (VDR) is expressed in human sperm, little is known about the role of vitamin D (Vit. D) in male reproduction. Our objective was to assess Vit. D levels both in serum and seminal fluid and to establish the relation between serum and seminal Vit. D levels, semen parameters, male sex hormones and serum calcium level in Egyptian infertile men. Patients and Methods: We conducted a prospective case control study including 30 healthy fertile males as a control group and 60 male patients with infertility of unknown etiology. Semen samples were collected and semen parameters were evaluated. Also, seminal Vit. D level was measured. Blood samples were taken as serum levels of Vit. D, calcium, testosterone, Luteinizing Hormone (LH) and Follicle-Stimulating Hormone (FSH) were estimated. Results: There was significant decrease of both serum and seminal Vit. D level in groups of male infertility compared to control group. A significant positive correlation was found between serum and seminal Vit. D levels in different study groups. Also, significant positive correlation between serum Vit. D level and non-progressive sperm motility. Conclusion: Our results support the role of Vit. D in semen parameters and male fertility status.

scholarly journals Effects of Weekly Supplementation of Cholecalciferol and Calcifediol Among the Oldest-Old People: Findings From a Randomized Pragmatic Clinical Trial

Nutrients ◽  
2019 ◽  
Vol 11 (11) ◽  
pp. 2778 ◽  
Author(s):  
Carmelinda Ruggiero ◽  
Marta Baroni ◽  
Vittorio Bini ◽  
Annalisa Brozzetti ◽  
Luca Parretti ◽  
...  
Keyword(s):  
Vitamin D ◽  
Handgrip Strength ◽  
Oldest Old ◽  
Serum Levels ◽  
Vitamin D Supplementation ◽  
Vitamin D Metabolites ◽  
Clinical Predictors ◽  
Pragmatic Clinical Trial ◽  
Reactive Protein ◽  
Time Interaction

Vitamin D inadequacy is pervasive in the oldest-old. Many vitamin D metabolites are available for supplementation, their effects on the recovery of adequate serum levels remain unknown. We investigate the effects of supplementation with cholecalciferol (D3) and calcifediol (25D3) on serum levels of 25(OH)D, 1-25(OH)D, bone and inflammatory markers, ultimately identifying clinical predictors of successful treatment. Sixty-seven oldest-old individuals were randomized to weekly administration of 150 mcg of 25D3 or D3, from hospital admission to 7 months after discharge. Supplementation of 25D3 and D3 were associated with increasing serum levels of 25(OH)D (p < 0.001) and 1-25(OH)D (p = 0.01). Participants on 25D3 experienced a steeper rise than those on D3 (group*time interaction p = 0.01), after adjustment for intact parathyroid hormone (iPTH) levels the differences disappeared (intervention*iPTH interaction p = 0.04). Vitamin D supplementation was associated with a decreasing trend of iPTH and C-reactive protein (CRP) (p < 0.001). Polypharmacy and low handgrip strength were predictors of failure of intervention, independent of vitamin D metabolites. In conclusion, D3 and 25D3 supplementation significantly increase vitamin D serum levels in the oldest-old individuals, with a tendency of 25D3 to show a faster recovery of acceptable iPTH levels than D3. Polypharmacy and low muscle strength weaken the recovery of adequate vitamin D serum levels.

scholarly journals Vitamin D Supplementation for Premenstrual Syndrome-Related inflammation and antioxidant markers in students with vitamin D deficient: a randomized clinical trial

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Hajar Heidari ◽  
Reza Amani ◽  
Awat Feizi ◽  
Gholamreza Askari ◽  
Shahnaz Kohan ◽  
...  
Keyword(s):  
Clinical Trial ◽  
Vitamin D ◽  
Randomized Clinical Trial ◽  
Premenstrual Syndrome ◽  
Serum Levels ◽  
Vitamin D Supplementation ◽  
Reproductive Age ◽  
Post Intervention ◽  
Total Antioxidant ◽  
Antioxidant Markers

Abstract Premenstrual syndrome (PMS) is a common disorder in the reproductive age that negatively significant impacts on women’s quality of life. This randomized clinical trial study was undertaken to investigate the effect of vitamin D supplementation on inflammatory and antioxidant markers in 44 vitamin D deficient (25(OH)D < 20 ng/mL) students with PMS. Participants received either 50,000 IU vitamin D3 or a placebo pearl fortnightly for 4 months. At the baseline and in the last 2 months of intervention, participants were asked to complete the PMS Daily Symptoms Rating form along with taking the pearls and their blood samples were collected to assess serum levels of 25(OH)D3, Interleukin10 and 12 (IL-10, IL-12) and total antioxidant capacity (TAC). In vitamin D group, serum levels of IL-10 and IL-12 significantly decreased while TAC significantly increased post-intervention. There were significant differences regarding serum IL-12 and TAC levels between the two groups. Mean score of the total PMS symptoms showed significant improvement in 25(OH)D. Vitamin D supplementation seems to be an effective strategy to improve inflammation and antioxidant markers in vitamin D deficient women with PMS. This clinical trial was registered at Iranian Registry of Clinical Trials on 20/06/2018 (IRCT20180525039822N1).

Prevalence of 25-hydroxyvitamin D deficiency in subgroups of elderly persons with anemia: association with anemia of inflammation

Blood ◽  
2011 ◽  
Vol 117 (10) ◽  
pp. 2800-2806 ◽  
Author(s):  
Todd S. Perlstein ◽  
Reena Pande ◽  
Nancy Berliner ◽  
Gary J. Vanasse
Keyword(s):  
Vitamin D ◽  
Vitamin D Deficiency ◽  
Serum Levels ◽  
The Elderly ◽  
Vitamin D Supplementation ◽  
World Health ◽  
Elderly Persons ◽  
Increased Risk ◽  
Health Organization ◽  
Anemia Of Inflammation

AbstractAnemia and vitamin D deficiency are conditions that both result in significant morbidity and increase with age. The potential relationship between them remains poorly understood, particularly in the elderly. We used the Third National Health and Nutrition Examination Survey to examine the association of vitamin D deficiency with anemia subtypes in persons aged ≥ 60 years. Vitamin D deficiency was defined as serum levels < 20 ng/mL, and anemia was defined according to World Health Organization criteria. Vitamin D deficiency was associated with anemia prevalence independent of age, sex, or race/ethnicity (odds ratio, 1.47; 95% confidence interval, 1.06-2.05; P = .02) and varied significantly by anemia subtype (P overall = .003). The prevalence of vitamin D deficiency was 33.3% in the nonanemic population, 56% in anemia of inflammation (AI; P = .008), and 33.0% in unexplained anemia (P = .55). Non-Hispanic blacks had a 7-fold increased risk of AI compared with whites, and this was partially attenuated after adjusting for vitamin D deficiency. These data show that vitamin D deficiency is associated with specific subtypes of anemia in the elderly, especially in those with AI. Vitamin D may suppress inflammatory pathways, and studies to determine whether vitamin D supplementation ameliorates AI are warranted.

189 THE EFFECT OF VITAMIN D SUPPLEMENTATION ON ANTI-MULLERIAN HORMONE LEVELS IN REPRODUCTIVE-AGE WOMEN

2015 ◽  
Vol 27 (1) ◽  
pp. 185 ◽  
Author(s):  
M. Taheri ◽  
M. Modarres ◽  
A. Abdollahi
Keyword(s):  
Vitamin D ◽  
Vitamin D Deficiency ◽  
Intervention Group ◽  
Serum Levels ◽  
Vitamin D Supplementation ◽  
T Test ◽  
Reproductive Age ◽  
Control Group ◽  
Post Intervention ◽  
Reproductive Age Women

Vitamin D deficiency has been correlated with the infertility and lower clinical pregnancy following IVF. Anti-Mullerian hormone (AMH) plays a key role during follicle development; it has been recognised as a predictor of regular ovulation and probably IVF success. Considering the critical need for experimental human study to investigate the impact of vitamin D supplementation on ovulatory function, the aim of this study was to demonstrate the effectiveness of the vitamin D supplementation on AMH serum levels among reproductive-age women with vitamin D deficiency. 195 reproductive women (18–35 year-old) with confirmed vitamin D deficiency [serum 25(OH)D <75 nmol L–1] and without diagnosed polycystic ovary syndrome (PCOS) were enrolled to this controlled clinical trial. Participants were randomly assigned to a control group (n = 96) or an intervention group (n = 99). Women in the intervention group used 2000 IU day–1 vitamin D drops for 15 weeks. 19 participants were missed during the follow-up; finally the numbers of women in the intervention and control groups were 91 and 85, respectively. At the beginning of the study and after the intervention, 25-hydroxyvitamin D and AMH serum levels were quantified using enzyme immunoassay (EIA; Immunodiagnostic Systems, Boldon, UK) and ELISA (Beckman-Coulter Inc., Fullerton, CA, USA) methods respectively. The post-intervention AMH measurement was performed after 2–5 weeks in the same day-of-cycle on which basal AMH measurement was done. Paired t-test, independent t-test, and Pearson correlation were used as appropriate and a P-value of less than 0.05 was considered significant. Significantly low AMH levels were seen in the vitamin D deficient women of this study (14.46 ± 11.92 pmol L–1 in control group and 14.09 ± 11.52 pmol L–1 in intervention group). After the intake of vitamin D supplementation in intervention group, AMH levels were increased to 24.89 ± 12.47 pmol L–1, which were significantly different from the 15.43 ± 13.03 pmol L–1 in control group (P < 0.001). Correlation coefficients for AMH with pre-intervention and post-intervention vitamin D were r = 0.489 and r = 0.599 respectively (P < 0.001). Treatment of vitamin D deficiency increases AMH to the optimum levels. Vitamin D deficient women had low levels of AMH. These findings support other studies which found a correlation of poor IVF outcomes with low vitamin D levels. Vitamin D supplementation could be useful in the improvement of controlled ovarian hyper-stimulation/IVF outcomes in case of vitamin D deficiency.

scholarly journals The Effects of Vitamin D Supplementation on Lipid and Inflammatory Profile of Healthy Adolescent Boys: A Randomized Controlled Trial

Nutrients ◽  
2020 ◽  
Vol 12 (5) ◽  
pp. 1213
Author(s):  
Amirhossein Yarparvar ◽  
Ibrahim Elmadfa ◽  
Abolghasem Djazayery ◽  
Zahra Abdollahi ◽  
Forouzan Salehi ◽  
...  
Keyword(s):  
Vitamin D ◽  
Lipid Profile ◽  
Controlled Trial ◽  
Group Analysis ◽  
Serum Levels ◽  
Vitamin D Supplementation ◽  
Inflammatory Biomarkers ◽  
Adolescent Boys ◽  
Healthy Adolescent ◽  
School Based

Background: Deficiency of vitamin D, an anti-inflammatory micronutrient with some favorable effects on lipid profiles, has been found to be highly prevalent in adolescents. We aimed to investigate the effect of a school-based vitamin D supplementation regimen on the correction of vitamin D deficiency as well as lipid and inflammatory profiles of healthy adolescent boys. Methods: In this randomized single-blind placebo-controlled trial, seventy-one healthy adolescent boys (age 17 years old) were recruited from one high school in Tehran, Iran, and randomly assigned to two groups. The supplement group received vitamin D pearls at a dose of 50,000 IU monthly for 6 months, this dose is indeed defined by the Ministry of Health in Iran for a potential national school-based vitamin D supplementation program. The other group was given placebo pearls for the same duration. Before and after the treatment, the serum levels of 25-hydroxy vitamin D (25(OH) D), parathyroid hormone (PTH), retinol, lead (Pb), the lipid profile and the inflammatory biomarkers were measured and compared. Results: Between-groups statistical analysis showed that a dose (50,000 IU/month) vitamin D significantly increased the serum levels of 25-hydroxyvitamin D (25 (OH) D) (p < 0.001) and decreased serum levels of PTH (p = 0.003). No significant change was observed in serum levels of retinol and Pb. Between-group analysis revealed that the serum levels of TG (P = 0.001) decreased while an increase in serum levels of HDL (p = 0.021) was observed (p < 0.05). Both the within- and between-group analysis showed that serum tumor necrosis factor receptor 2 (TNFR2) concentration declined while serum interleukin-10 (IL-10) increased in response to vitamin D supplementation (p < 0.05). Conclusion: A supplementation regimen of (50,000 IU/month) vitamin D in a context with high rates of vitamin deficiency has shown positive impacts on the serum vitamin D, lipid profile and inflammatory biomarkers in healthy adolescent boys.

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