scholarly journals Carbon nanomaterial-derived lung burden analysis using UV-Vis spectrophotometry and proteinase K digestion

2020 ◽  
Vol 17 (1) ◽  
Author(s):  
Dong-Keun Lee ◽  
Soyeon Jeon ◽  
Jiyoung Jeong ◽  
Kyung Seuk Song ◽  
Wan-Seob Cho

Abstract Background The quantification of nanomaterials accumulated in various organs is crucial in studying their toxicity and toxicokinetics. However, some types of nanomaterials, including carbon nanomaterials (CNMs), are difficult to quantify in a biological matrix. Therefore, developing improved methodologies for quantification of CNMs in vital organs is instrumental in their continued modification and application. Results In this study, carbon black, nanodiamond, multi-walled carbon nanotube, carbon nanofiber, and graphene nanoplatelet were assembled and used as a panel of CNMs. All CNMs showed significant absorbance at 750 nm, while their bio-components showed minimal absorbance at this wavelength. Quantification of CNMs using their absorbance at 750 nm was shown to have more than 94% accuracy in all of the studied materials. Incubating proteinase K (PK) for 2 days with a mixture of lung tissue homogenates and CNMs showed an average recovery rate over 90%. The utility of this method was confirmed in a murine pharyngeal aspiration model using CNMs at 30 μg/mouse. Conclusions We developed an improved lung burden assay for CNMs with an accuracy > 94% and a recovery rate > 90% using PK digestion and UV-Vis spectrophotometry. This method can be applied to any nanomaterial with sufficient absorbance in the near-infrared band and can differentiate nanomaterials from elements in the body, as well as the soluble fraction of the nanomaterial. Furthermore, a combination of PK digestion and other instrumental analysis specific to the nanomaterial can be applied to organ burden analysis.

Energies ◽  
2021 ◽  
Vol 14 (14) ◽  
pp. 4158
Author(s):  
Haiyan Yu ◽  
Haochun Zhang ◽  
Heming Wang ◽  
Dong Zhang

Currently, there are few studies on the influence of microscale thermal radiation on the equivalent thermal conductivity of microscale porous metal. Therefore, this paper calculated the equivalent thermal conductivity of high-porosity periodic cubic silver frame structures with cell size from 100 nm to 100 µm by using the microscale radiation method. Then, the media radiation characteristics, absorptivity, reflectivity and transmissivity were discussed to explain the phenomenon of the radiative thermal conductivity changes. Furthermore, combined with spectral radiation properties at the different cross-sections and wavelength, the radiative transmission mechanism inside high-porosity periodic cubic frame silver structures was obtained. The results showed that the smaller the cell size, the greater radiative contribution in total equivalent thermal conductivity. Periodic cubic silver frames fluctuate more in the visible band and have better thermal radiation modulation properties in the near infrared band, which is formed by the Surface Plasmon Polariton and Magnetic Polaritons resonance jointly. This work provides design guidance for the application of this kind of periodic microporous metal in the field of thermal utilization and management.


2021 ◽  
Vol 9 (1) ◽  
Author(s):  
Ning Cao ◽  
Shuqiang Lyu ◽  
Miaole Hou ◽  
Wanfu Wang ◽  
Zhenhua Gao ◽  
...  

AbstractEnvironmental changes and human activities can cause serious degradation of murals, where sootiness is one of the most common problems of ancient Chinese indoor murals. In order to improve the visual quality of the murals, a restoration method is proposed for sootiness murals based on dark channel prior and Retinex by bilateral filter using hyperspectral imaging technology. First, radiometric correction and denoising through band clipping and minimum noise fraction rotation forward and inverse transform were applied to the hyperspectral data of the sootiness mural to produce its denoised reflectance image. Second, a near-infrared band was selected from the reflectance image and combined with the green and blue visible bands to produce a pseudo color image for the subsequent sootiness removal processing. The near-infrared band is selected because it is better penetrating the sootiness layer to a certain extent comparing to other bands. Third, the sootiness covered on the pseudo color image was preliminarily removed by using the method of dark channel prior and by adjusting the brightness of the image. Finally, the Retinex by bilateral filter was performed on the image to get the final restored image, where the sootiness was removed. The results show that the images restored by the proposed method are superior in variance, average gradient, information entropy and gray scale contrast comparing to the results from the traditional methods of homomorphic filtering and Gaussian stretching. The results also show the highest score in comprehensive evaluation of edges, hue and structure; thus, the method proposed can support more potential studies or sootiness removal in real mural paintings with more detailed information. The method proposed shows strong evidence that it can effectively reduce the influence of sootiness on the moral images with more details that can reveal the original appearance of the mural and improve its visual quality.


2021 ◽  
Vol 48 (7) ◽  
pp. 0706001
Author(s):  
张文 Zhang Wen ◽  
白冰冰 Bai Bingbing ◽  
张砚曾 Zhang Yanzeng ◽  
陈聪 Chen Cong ◽  
邵齐元 Shao Qiyuan ◽  
...  

2021 ◽  

Hypoxic-ischemic brain injury (HIBI) is a leading cause of mortality in post-cardiac arrest (post-CA) patients who successfully survive the initial cardiopulmonary resuscitation (CPR) but later die in the Intensive Care Unit (ICU). Therefore, a key priority of post-resuscitation ICU care is to prevent and limit the impact of HIBI by optimizing the balance between cerebral oxygen delivery and demand. Traditionally, an optimal systemic oxygen balance is considered to ensure the brain’s oxygen balance. However, the validity of this assumption is uncertain, as the brain constitutes only 2%of the body mass while accounting for approximately 20% of basal oxygen consumption at rest. Hence, there is a real need to monitor cerebral oxygenation realistically. Several imaging and bedside monitoring methods are available for cerebral oxygenation monitoring in post-CA patients. Unfortunately, each of them has its limitations. Imaging methods require transporting a critically ill unstable patient to the scanner. Moreover, they provide an assessment of the oxygenation state only at a particular moment, while brain oxygenation is dynamic. Bedside methods, specifically near-infrared spectroscopy (NIRS), brain tissue oxygen tension (PbtO2), and jugular venous oxygen saturation monitoring (SjvO2), have not often been used in studies involving post-CA patients. Hence there is ambiguity regarding clear recommendations for using these bedside monitors. Presently, the most promising option seems to be using the NIRS as an indicator of effective CPR. We present a narrative review focusing on bedside methods and discuss the evidence for their use in adult patients after cardiac arrest.


2011 ◽  
Vol 20 (03) ◽  
pp. 687-695 ◽  
Author(s):  
DOMINICK J. BINDL ◽  
MICHAEL S. ARNOLD

A photovoltaic photodetector harnessing near infrared band gap absorption by thin films of post-synthetically sorted semiconducting single walled carbon nanotubes ( s -SWCNTs) is described. Peak specific detectivity of 6×1011 Jones at -0.1 V bias at 1210 nm is achieved using a heterojunction device architecture: indium tin oxide/ ca. 5 nm s -SWCNT / 120 nm C60 / 10 nm 2,9-dimethyl-4,7-diphenyl-1,10-phenanthroline (BCP) / Ag. The photodiodes are characterized by a series resistance of 2.9 Ω cm2 and a rectification ratio of 104 at ±1V. These results are expected to guide the exploration of new classes of solution-processable, mechanically flexible, integrable, thin film photovoltaic photodetectors with tunable sensitivity in the visible and infrared spectra based on semiconducting carbon nanotubes.


2022 ◽  
Vol 14 (2) ◽  
pp. 386
Author(s):  
Léa Schamberger ◽  
Audrey Minghelli ◽  
Malik Chami ◽  
François Steinmetz

The invasive species of brown algae Sargassum gathers in large aggregations in the Caribbean Sea, and has done so especially over the last decade. These aggregations wash up on shores and decompose, leading to many socio-economic issues for the population and the coastal ecosystem. Satellite ocean color data sensors such as Sentinel-3/OLCI can be used to detect the presence of Sargassum and estimate its fractional coverage and biomass. The derivation of Sargassum presence and abundance from satellite ocean color data first requires atmospheric correction; however, the atmospheric correction procedure that is commonly used for oceanic waters needs to be adapted when dealing with the occurrence of Sargassum because the non-zero water reflectance in the near infrared band induced by Sargassum optical signature could lead to Sargassum being wrongly identified as aerosols. In this study, this difficulty is overcome by interpolating aerosol and sunglint reflectance between nearby Sargassum-free pixels. The proposed method relies on the local homogeneity of the aerosol reflectance between Sargassum and Sargassum-free areas. The performance of the adapted atmospheric correction algorithm over Sargassum areas is evaluated. The proposed method is demonstrated to result in more plausible aerosol and sunglint reflectances. A reduction of between 75% and 88% of pixels showing a negative water reflectance above 600 nm were noticed after the correction of the several images.


2021 ◽  
Vol 236 ◽  
pp. 02033
Author(s):  
Xiaoyan Ge ◽  
Qian Mo ◽  
Guofeng Wang ◽  
Yan Zhao ◽  
Yongyu Li ◽  
...  

Antimony (Sb) is a toxic and potentially carcinogenic metalloid element. The toxicity of Sb in the environment strongly relies on its speciation. It is necessary to investigate the speciation and content of antimony in soil in order to understand better the real risk associated with Sb in the environment. This study developed water bath heating and soaking-AFS methods to determine the available and carbonate antimony in soil. Through analysis and mutual verification experiments in three different laboratories, the repeatability and reproducibility in the precision experiment were less than 3.44, the average recovery rate was more than 98.1%. These methods were accurate, reproducible and effective for detecting the content of available and carbonate antimony in soil.


2021 ◽  
Vol 893 (1) ◽  
pp. 012068
Author(s):  
K I N Rahmi ◽  
N Febrianti ◽  
I Prasasti

Abstract Forest/land fire give bad impact of heavy smoke on peatland area in Indonesia. Forest/land fire smoke need to be identified the distribution periodically. New satellite of GCOM-C has been launched to monitor climate condition and have visible, near infrared and thermal infrared. This study has objective to identify fire smoke from GCOM-C data. GCOM-C data has wavelength range from 0.38 to 12 μm it covers visible, near infrared, short-wave infrared and thermal infrared. It is relatively similar to MODIS or Himawari-8 images which could identify forest/land fire smoke. The methodology is visual interpretation to detect forest/land fire smoke using near infrared band (VN08), shortwave infrared band (SW03), and thermal bands (T01 and T02). Hotspot data is overlaid with GCOM-C image to represent the location of fire events. Combination of composite RGB image has been applied to detect forest/land fire smoke. GCOM-C image of VN8 bands and combination of thermal band in composite image could be used to detect fire smoke in Pulang Pisau, Central Kalimantan.


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