scholarly journals Whole conversion of agro-industrial wastes rich in galactose-based carbohydrates into lipid using oleaginous yeast Aureobasidium namibiae

2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Zhi-Peng Wang ◽  
Xin-Yue Zhang ◽  
Yan Ma ◽  
Jing-Run Ye ◽  
Jing Jiang ◽  
...  
Keyword(s):  
Oleaginous Yeast ◽  
Raw Materials ◽  
Lipid Production ◽  
Industrial Wastes ◽  
Lactose Hydrolysis ◽  
Oleaginous Yeasts ◽  
Soy Molasses ◽  
First Time ◽  
Hydrolysis Of ◽  
Direct Hydrolysis

Abstract Background Raw materials composed of easily assimilated monosaccharides have been employed as carbon source for production of microbial lipids. Nevertheless, agro-industrial wastes rich in galactose-based carbohydrates have not been introduced as feedstocks for oleaginous yeasts. Results In this study, Aureobasidium namibiae A12 was found to efficiently accumulate lipid from soy molasses and whey powder containing galactose-based carbohydrates, with lipid productions of 5.30 g/L and 5.23 g/L, respectively. Over 80% of the fatty acids was C16:0, C18:0, C18:1, and C18:2. All kinds of single sugar components in the two byproducts were readily converted into lipids, with yields ranging between 0.116 g/g and 0.138 g/g. Three α-galactosidases and five β-galactosidases in the strain were cloned and analyzed. Changes of transcriptional levels indicated GalB and GalC were key α-galactosidases, and GalG was key β-galactosidase. In 10 L fermentor, lipid production from SM and WP achieved 6.45 g/L and 6.13 g/L, respectively. β-galactosidase was responsible for lactose hydrolysis; sucrase and α-galactosidase both contributed to the efficient hydrolysis of raffinose and stachyose in a cooperation manner. Conclusions This is a new way to produce lipids from raw materials containing galactose-based carbohydrates. This finding revealed the significance of sucrase in the direct hydrolysis of galactose-based carbohydrates in raw materials for the first time and facilitated the understanding of the efficient utilization of galactose-based carbohydrates to manufacture lipid or other chemicals in bioprocess. Graphic abstract

scholarly journals Whole Conversion of Agro-Industrial Wastes Rich in Galactose-Based Carbohydrates into Lipid Using Oleaginous Yeast Aureobasidium Namibiae

2021 ◽  
Author(s):  
Zhi-Peng Wang ◽  
Jing-Run Ye ◽  
Yan Ma ◽  
Xin-Yue Zhang ◽  
Hai-ying Wang ◽  
...  
Keyword(s):  
Oleaginous Yeast ◽  
Raw Materials ◽  
Industrial Wastes ◽  
Lactose Hydrolysis ◽  
Efficient Utilization ◽  
Oleaginous Yeasts ◽  
Soy Molasses ◽  
First Time ◽  
Hydrolysis Of ◽  
Direct Hydrolysis

Abstract BackgroundRaw materials composed of easily assimilated monosaccharides have been employed as carbon source for production of microbial lipids. Nevertheless, agro-industrial wastes rich in galactose-based carbohydrates have not been introduced as feedstocks for oleaginous yeasts. ResultsIn this study, Aureobasidium namibiae A12 was found to efficiently accumulate lipid from soy molasses and whey powder containing galactose-based carbohydrates, with lipid productions of 5.30 g/L and 5.23 g/L, respectively. Over 80% of the fatty acids was C16:0, C18:0, C18:1, and C18:2. All kinds of single sugar components in the two byproducts were readily converted into lipids, with yields ranging between 0.116 g/g and 0.138 g/g. Three α-galactosidases and five β-galactosidases in the strain were cloned and analyzed. β-galactosidase was responsible for lactose hydrolysis; sucrase and α-galactosidase both contributed to the efficient hydrolysis of raffinose and stachyose in a cooperation manner. ConclusionsThis is a new way to produce lipids from raw materials containing galactose-based carbohydrates. This finding revealed the significance of sucrase in the direct hydrolysis of galactose-based carbohydrates in raw materials for the first time and facilitated the understanding of the efficient utilization of galactose-based carbohydrates to manufacture lipid or other chemicals in bioprocess

scholarly journals Acid Hydrolysis of Brewers’ Industrial Wastes and Their Use for Lipid Production by Oleaginous Yeasts

2019 ◽  
Vol 17 (5) ◽  
pp. 336-344
Author(s):  
Saithip Sae-ngae ◽  
Benjamas Cheirsilp ◽  
Thanwadee Tachapattaweawrakul Suksaroj ◽  
Punyanich Intharapat
Keyword(s):  
Acid Hydrolysis ◽  
Lipid Production ◽  
Industrial Wastes ◽  
Oleaginous Yeasts ◽  
Hydrolysis Of

Valorization of agro-industrial wastes to produce hydrolytic enzymes by fungal solid-state fermentation

2018 ◽  
Vol 37 (2) ◽  
pp. 149-156 ◽  
Author(s):  
C. Marzo ◽  
A.B. Díaz ◽  
I. Caro ◽  
A. Blandino
Keyword(s):  
Solid State ◽  
Solid State Fermentation ◽  
Reducing Sugars ◽  
Raw Materials ◽  
Hydrolytic Enzymes ◽  
Industrial Wastes ◽  
Added Value ◽  
Orange Peels ◽  
Enzyme Cocktail ◽  
Hydrolysis Of

Nowadays, significant amounts of agro-industrial wastes are discarded by industries; however, they represent interesting raw materials for the production of high-added value products. In this regard, orange peels (ORA) and exhausted sugar beet cossettes (ESBC) have turned out to be promising raw materials for hydrolytic enzymes production by solid state fermentation (SSF) and also a source of sugars which could be fermented to different high-added value products. The maximum activities of xylanase and exo-polygalacturonase (exo-PG) measured in the enzymatic extracts obtained after the SSF of ORA were 31,000 U·kg-1 and 17,600 U·kg-1, respectively; while for ESBC the maximum values reached were 35,000 U·kg-1 and 28,000 U·kg-1, respectively. The enzymatic extracts obtained in the SSF experiments were also employed for the hydrolysis of ORA and ESBC. Furthermore, it was found that extracts obtained from SSF of ORA, supplemented with commercial cellulase, were more efficient for the hydrolysis of ORA and ESBC than a commercial enzyme cocktail typically used for this purpose. In this case, maximum reducing sugars concentrations of 57 and 47 g·L-1 were measured after the enzymatic hydrolysis of ESBC and ORA, respectively.

scholarly journals Lipid production through the single-step microwave hydrolysis of macroalgae using the oleaginous yeast Metschnikowia pulcherrima

2019 ◽  
Vol 38 ◽  
pp. 101411 ◽  
Author(s):  
Felix Abeln ◽  
Jiajun Fan ◽  
Vitaliy L. Budarin ◽  
Hannah Briers ◽  
Sophie Parsons ◽  
...  
Keyword(s):  
Oleaginous Yeast ◽  
Lipid Production ◽  
Single Step ◽  
Metschnikowia Pulcherrima ◽  
Microwave Hydrolysis ◽  
Hydrolysis Of

Towards high-throughput optimization of microbial lipid production: from strain development to process monitoring

2020 ◽  
Vol 4 (12) ◽  
pp. 5958-5969
Author(s):  
Dania Awad ◽  
Samer Younes ◽  
Matthias Glemser ◽  
Franz M. Wagner ◽  
Gerhard Schenk ◽  
...  
Keyword(s):  
Process Monitoring ◽  
High Throughput ◽  
Oleaginous Yeast ◽  
Lipid Production ◽  
Throughput Optimization ◽  
Microbial Lipid ◽  
Strain Development ◽  
Fast Neutron Irradiation ◽  
Microbial Lipid Production ◽  
First Time

We describe, for the first time, the use of fast neutron irradiation for the rapid metabolic optimization of an oleaginous yeast. The approach established in this study provides a progressive path towards automatable strain development efforts.

scholarly journals The history, state of the art and future prospects for oleaginous yeast research

2021 ◽  
Vol 20 (1) ◽  
Author(s):  
Felix Abeln ◽  
Christopher J. Chuck
Keyword(s):  
Industrial Production ◽  
Oleaginous Yeast ◽  
Process Development ◽  
Animal Feed ◽  
Lipid Production ◽  
Critical Discussion ◽  
Transport Sector ◽  
Oleaginous Yeasts ◽  
Promising Alternative ◽  
Tropical Regions

AbstractLipid-based biofuels, such as biodiesel and hydroprocessed esters, are a central part of the global initiative to reduce the environmental impact of the transport sector. The vast majority of production is currently from first-generation feedstocks, such as rapeseed oil, and waste cooking oils. However, the increased exploitation of soybean oil and palm oil has led to vast deforestation, smog emissions and heavily impacted on biodiversity in tropical regions. One promising alternative, potentially capable of meeting future demand sustainably, are oleaginous yeasts. Despite being known about for 143 years, there has been an increasing effort in the last decade to develop a viable industrial system, with currently around 100 research papers published annually. In the academic literature, approximately 160 native yeasts have been reported to produce over 20% of their dry weight in a glyceride-rich oil. The most intensively studied oleaginous yeast have been Cutaneotrichosporon oleaginosus (20% of publications), Rhodotorula toruloides (19%) and Yarrowia lipolytica (19%). Oleaginous yeasts have been primarily grown on single saccharides (60%), hydrolysates (26%) or glycerol (19%), and mainly on the mL scale (66%). Process development and genetic modification (7%) have been applied to alter yeast performance and the lipids, towards the production of biofuels (77%), food/supplements (24%), oleochemicals (19%) or animal feed (3%). Despite over a century of research and the recent application of advanced genetic engineering techniques, the industrial production of an economically viable commodity oil substitute remains elusive. This is mainly due to the estimated high production cost, however, over the course of the twenty-first century where climate change will drastically change global food supply networks and direct governmental action will likely be levied at more destructive crops, yeast lipids offer a flexible platform for localised, sustainable lipid production. Based on data from the large majority of oleaginous yeast academic publications, this review is a guide through the history of oleaginous yeast research, an assessment of the best growth and lipid production achieved to date, the various strategies employed towards industrial production and importantly, a critical discussion about what needs to be built on this huge body of work to make producing a yeast-derived, more sustainable, glyceride oil a commercial reality.

scholarly journals Cutaneotrichosporon oleaginosus: A Versatile Whole-Cell Biocatalyst for the Production of Single-Cell Oil from Agro-Industrial Wastes

Catalysts ◽  
2021 ◽  
Vol 11 (11) ◽  
pp. 1291
Author(s):  
Nicola Di Fidio ◽  
Filippo Minonne ◽  
Claudia Antonetti ◽  
Anna Maria Raspolli Galletti
Keyword(s):  
Single Cell ◽  
Oleaginous Yeast ◽  
State Of The Art ◽  
Lipid Production ◽  
Industrial Applications ◽  
Industrial Wastes ◽  
Single Cell Oil ◽  
Whole Cell ◽  
Whole Cell Biocatalyst ◽  
By Products

Cutaneotrichosporon oleaginosus is an oleaginous yeast with several favourable qualities: It is fast growing, accumulates high amounts of lipids and has a very broad substrate spectrum. Its resistance to hydrolysis by-products makes it a promising biocatalyst for custom tailored microbial oils. C. oleaginosus can accumulate up to 60 wt.% of its biomass as lipids. This species is able to grow by using several compounds as a substrate, such as acetic acid, biodiesel-derived glycerol, N-acetylglucosamine, lignocellulosic hydrolysates, wastepaper and other agro-industrial wastes. This review is focused on state-of-the-art innovative and sustainable biorefinery schemes involving this promising yeast and second- and third-generation biomasses. Moreover, this review offers a comprehensive and updated summary of process strategies, biomass pretreatments and fermentation conditions for enhancing lipid production by C. oleaginosus as a whole-cell biocatalyst. Finally, an overview of the main industrial applications of single-cell oil is reported together with future perspectives.

ON THE PROCESSING OF HEMATITE RAW MATERIALS AT THE KRYVOROZHSK MINING AND PROCESSING PLANT OF OXIDIZED ORE ON TECHNOLOGY OF DIRECT RECOVERY ITmk3

2018 ◽  
pp. 32-37
Author(s):  
P.I. Loboda ◽  
Younes Razaz ◽  
S. Grishchenko
Keyword(s):  
Magnetic Properties ◽  
Cast Iron ◽  
Iron Content ◽  
Raw Materials ◽  
Direct Reduction ◽  
Processing Plant ◽  
High Iron Content ◽  
High Iron ◽  
Direct Restoration ◽  
First Time

Purpose. To substantiate the efficiency of processing hematite raw materials at the Krivoy Rog Mining and Processing Plant of Oxidized Ores using the direct reduction technology itmk3®. Metodology. Analysis of the results of the itmk3® direct restoration technology developed by Kobe Steel Ltd., Japan and Hares Engineering GmbX, Austria, with a view to using it to process Krivbass hematite ores into granulated iron (so-called “nuggets”). Findings. The involvement in the production of hematite ores (oxidized quartzite) of Krivbass with high iron content, but with low magnetic properties for their processing into granular cast iron is grounded. Originality. The use of itmk3® direct reduction technology from Kobe Steel Ltd., Japan and Hares Engineering GmbH, Austria for the processing of Krivbass hematite ores into granular cast iron is justified for the first time. Practical value. The efficiency of the use of hematite ores (oxidized quartzite) has been substantiated, which can significantly reduce the costs in the mining cycle for the economical production of metallurgical products.

PERSPECTIVE DEVELOPMENT OF WORLD PRODUCTION OF FEEDS FOR AQUACULTURE: ALTERNATIVE SOURCES OF RAW MATERIALS

2017 ◽  
pp. 67-78
Author(s):  
Lina Yurievna Lagutkina
Keyword(s):  
Raw Materials ◽  
Limiting Factors ◽  
Current Status ◽  
Feed Production ◽  
The World ◽  
Market Trends ◽  
Alternative Sources ◽  
World Industry ◽  
First Time ◽  
A Current

The author of the article discloses the prospects of development of the world feed production for aquaculture based on the analysis of key innovative technological and market trends. The author specifies that shortage, high cost, low ecological compatibility of traditional raw materials - fish flour - are among major limiting factors in the development of production of feeds for aquaculture. This fact, in turn, limits sustainable development of aquaculture both in Russia, and in the world in general. The article presents the overview of a current status of the world industry of feed production in aquaculture, where the regional situation is studied, as well. For the first time, there is given the outlook of innovative technologies in feed production based on the alternative sources of protein (on the example of projects of leading aquabiotechnological companies) which will determine industry’s objectives for the mid-term perspective.

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