scholarly journals Whole Conversion of Agro-Industrial Wastes Rich in Galactose-Based Carbohydrates into Lipid Using Oleaginous Yeast Aureobasidium Namibiae

2021 ◽  
Author(s):  
Zhi-Peng Wang ◽  
Jing-Run Ye ◽  
Yan Ma ◽  
Xin-Yue Zhang ◽  
Hai-ying Wang ◽  
...  
Keyword(s):  
Oleaginous Yeast ◽  
Raw Materials ◽  
Industrial Wastes ◽  
Lactose Hydrolysis ◽  
Efficient Utilization ◽  
Oleaginous Yeasts ◽  
Soy Molasses ◽  
First Time ◽  
Hydrolysis Of ◽  
Direct Hydrolysis

Abstract BackgroundRaw materials composed of easily assimilated monosaccharides have been employed as carbon source for production of microbial lipids. Nevertheless, agro-industrial wastes rich in galactose-based carbohydrates have not been introduced as feedstocks for oleaginous yeasts. ResultsIn this study, Aureobasidium namibiae A12 was found to efficiently accumulate lipid from soy molasses and whey powder containing galactose-based carbohydrates, with lipid productions of 5.30 g/L and 5.23 g/L, respectively. Over 80% of the fatty acids was C16:0, C18:0, C18:1, and C18:2. All kinds of single sugar components in the two byproducts were readily converted into lipids, with yields ranging between 0.116 g/g and 0.138 g/g. Three α-galactosidases and five β-galactosidases in the strain were cloned and analyzed. β-galactosidase was responsible for lactose hydrolysis; sucrase and α-galactosidase both contributed to the efficient hydrolysis of raffinose and stachyose in a cooperation manner. ConclusionsThis is a new way to produce lipids from raw materials containing galactose-based carbohydrates. This finding revealed the significance of sucrase in the direct hydrolysis of galactose-based carbohydrates in raw materials for the first time and facilitated the understanding of the efficient utilization of galactose-based carbohydrates to manufacture lipid or other chemicals in bioprocess

scholarly journals Whole conversion of agro-industrial wastes rich in galactose-based carbohydrates into lipid using oleaginous yeast Aureobasidium namibiae

2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Zhi-Peng Wang ◽  
Xin-Yue Zhang ◽  
Yan Ma ◽  
Jing-Run Ye ◽  
Jing Jiang ◽  
...  
Keyword(s):  
Oleaginous Yeast ◽  
Raw Materials ◽  
Lipid Production ◽  
Industrial Wastes ◽  
Lactose Hydrolysis ◽  
Oleaginous Yeasts ◽  
Soy Molasses ◽  
First Time ◽  
Hydrolysis Of ◽  
Direct Hydrolysis

Abstract Background Raw materials composed of easily assimilated monosaccharides have been employed as carbon source for production of microbial lipids. Nevertheless, agro-industrial wastes rich in galactose-based carbohydrates have not been introduced as feedstocks for oleaginous yeasts. Results In this study, Aureobasidium namibiae A12 was found to efficiently accumulate lipid from soy molasses and whey powder containing galactose-based carbohydrates, with lipid productions of 5.30 g/L and 5.23 g/L, respectively. Over 80% of the fatty acids was C16:0, C18:0, C18:1, and C18:2. All kinds of single sugar components in the two byproducts were readily converted into lipids, with yields ranging between 0.116 g/g and 0.138 g/g. Three α-galactosidases and five β-galactosidases in the strain were cloned and analyzed. Changes of transcriptional levels indicated GalB and GalC were key α-galactosidases, and GalG was key β-galactosidase. In 10 L fermentor, lipid production from SM and WP achieved 6.45 g/L and 6.13 g/L, respectively. β-galactosidase was responsible for lactose hydrolysis; sucrase and α-galactosidase both contributed to the efficient hydrolysis of raffinose and stachyose in a cooperation manner. Conclusions This is a new way to produce lipids from raw materials containing galactose-based carbohydrates. This finding revealed the significance of sucrase in the direct hydrolysis of galactose-based carbohydrates in raw materials for the first time and facilitated the understanding of the efficient utilization of galactose-based carbohydrates to manufacture lipid or other chemicals in bioprocess. Graphic abstract

Valorization of agro-industrial wastes to produce hydrolytic enzymes by fungal solid-state fermentation

2018 ◽  
Vol 37 (2) ◽  
pp. 149-156 ◽  
Author(s):  
C. Marzo ◽  
A.B. Díaz ◽  
I. Caro ◽  
A. Blandino
Keyword(s):  
Solid State ◽  
Solid State Fermentation ◽  
Reducing Sugars ◽  
Raw Materials ◽  
Hydrolytic Enzymes ◽  
Industrial Wastes ◽  
Added Value ◽  
Orange Peels ◽  
Enzyme Cocktail ◽  
Hydrolysis Of

Nowadays, significant amounts of agro-industrial wastes are discarded by industries; however, they represent interesting raw materials for the production of high-added value products. In this regard, orange peels (ORA) and exhausted sugar beet cossettes (ESBC) have turned out to be promising raw materials for hydrolytic enzymes production by solid state fermentation (SSF) and also a source of sugars which could be fermented to different high-added value products. The maximum activities of xylanase and exo-polygalacturonase (exo-PG) measured in the enzymatic extracts obtained after the SSF of ORA were 31,000 U·kg-1 and 17,600 U·kg-1, respectively; while for ESBC the maximum values reached were 35,000 U·kg-1 and 28,000 U·kg-1, respectively. The enzymatic extracts obtained in the SSF experiments were also employed for the hydrolysis of ORA and ESBC. Furthermore, it was found that extracts obtained from SSF of ORA, supplemented with commercial cellulase, were more efficient for the hydrolysis of ORA and ESBC than a commercial enzyme cocktail typically used for this purpose. In this case, maximum reducing sugars concentrations of 57 and 47 g·L-1 were measured after the enzymatic hydrolysis of ESBC and ORA, respectively.

scholarly journals Acid Hydrolysis of Brewers’ Industrial Wastes and Their Use for Lipid Production by Oleaginous Yeasts

2019 ◽  
Vol 17 (5) ◽  
pp. 336-344
Author(s):  
Saithip Sae-ngae ◽  
Benjamas Cheirsilp ◽  
Thanwadee Tachapattaweawrakul Suksaroj ◽  
Punyanich Intharapat
Keyword(s):  
Acid Hydrolysis ◽  
Lipid Production ◽  
Industrial Wastes ◽  
Oleaginous Yeasts ◽  
Hydrolysis Of

ON THE PROCESSING OF HEMATITE RAW MATERIALS AT THE KRYVOROZHSK MINING AND PROCESSING PLANT OF OXIDIZED ORE ON TECHNOLOGY OF DIRECT RECOVERY ITmk3

2018 ◽  
pp. 32-37
Author(s):  
P.I. Loboda ◽  
Younes Razaz ◽  
S. Grishchenko
Keyword(s):  
Magnetic Properties ◽  
Cast Iron ◽  
Iron Content ◽  
Raw Materials ◽  
Direct Reduction ◽  
Processing Plant ◽  
High Iron Content ◽  
High Iron ◽  
Direct Restoration ◽  
First Time

Purpose. To substantiate the efficiency of processing hematite raw materials at the Krivoy Rog Mining and Processing Plant of Oxidized Ores using the direct reduction technology itmk3®. Metodology. Analysis of the results of the itmk3® direct restoration technology developed by Kobe Steel Ltd., Japan and Hares Engineering GmbX, Austria, with a view to using it to process Krivbass hematite ores into granulated iron (so-called “nuggets”). Findings. The involvement in the production of hematite ores (oxidized quartzite) of Krivbass with high iron content, but with low magnetic properties for their processing into granular cast iron is grounded. Originality. The use of itmk3® direct reduction technology from Kobe Steel Ltd., Japan and Hares Engineering GmbH, Austria for the processing of Krivbass hematite ores into granular cast iron is justified for the first time. Practical value. The efficiency of the use of hematite ores (oxidized quartzite) has been substantiated, which can significantly reduce the costs in the mining cycle for the economical production of metallurgical products.

PERSPECTIVE DEVELOPMENT OF WORLD PRODUCTION OF FEEDS FOR AQUACULTURE: ALTERNATIVE SOURCES OF RAW MATERIALS

2017 ◽  
pp. 67-78
Author(s):  
Lina Yurievna Lagutkina
Keyword(s):  
Raw Materials ◽  
Limiting Factors ◽  
Current Status ◽  
Feed Production ◽  
The World ◽  
Market Trends ◽  
Alternative Sources ◽  
World Industry ◽  
First Time ◽  
A Current

The author of the article discloses the prospects of development of the world feed production for aquaculture based on the analysis of key innovative technological and market trends. The author specifies that shortage, high cost, low ecological compatibility of traditional raw materials - fish flour - are among major limiting factors in the development of production of feeds for aquaculture. This fact, in turn, limits sustainable development of aquaculture both in Russia, and in the world in general. The article presents the overview of a current status of the world industry of feed production in aquaculture, where the regional situation is studied, as well. For the first time, there is given the outlook of innovative technologies in feed production based on the alternative sources of protein (on the example of projects of leading aquabiotechnological companies) which will determine industry’s objectives for the mid-term perspective.

Safety assessment of biological raw materials in the system of medicines quality assurance

2020 ◽  
pp. 63-72
Author(s):  
Yu. Olefir ◽  
E. Sakanyan ◽  
I. Osipova ◽  
V. Dobrynin ◽  
M. Smirnova ◽  
...  
Keyword(s):  
Raw Materials ◽  
State Level ◽  
The State ◽  
Spongiform Encephalopathy ◽  
Safety Standards ◽  
Wide Range ◽  
The Russian Federation ◽  
Key Aspects ◽  
First Time ◽  
Medicines Quality

The entry of a wide range of biotechnological products into the pharmaceutical market calls for rein-forcement of the quality, efficacy and safety standards at the state level. The following general monographs have been elaborated for the first time to be included into the State Pharmacopoeia of the Russian Federation, XIV edition: "Viral safety" and "Reduction of the risk of transmitting animal spongiform encephalopathy via medicinal products". These general monographs were elaborated taking into account the requirements of foreign pharmacopoeias and the WHO recommendations. The present paper summarises the key aspects of the monographs.

DISTRIBUTION OF HEXOSAMINE IN ELEGTROPHORETIC FRACTIONS OF AVIAN, BOVINE, PORCINE, AND HUMAN SERA

1967 ◽  
Vol 45 (4) ◽  
pp. 541-550 ◽  
Author(s):  
A. P. Gaunce ◽  
P. A. Anastassiadis
Keyword(s):  
Human Blood ◽  
Filter Paper ◽  
Cationic Resin ◽  
Human Sera ◽  
Hydrolysis Of ◽  
Direct Hydrolysis

The distribution of hexosamine among the proteins of avian, bovine, porcine, and human blood sera was studied by electrophoresis on filter paper. Hexosamine was determined after direct hydrolysis of stained sections of the paper, followed by chromatography of hydrolysates on cationic resin. Some substantial and statistically significant differences in hexosamine and protein contents of the zones were found among species.

scholarly journals Location of Biorefineries Based on Olive-Derived Biomass in Andalusia, Spain

Energies ◽  
2021 ◽  
Vol 14 (11) ◽  
pp. 3052
Author(s):  
Diego Cardoza ◽  
Inmaculada Romero ◽  
Teresa Martínez ◽  
Encarnación Ruiz ◽  
Francisco J. Gallego ◽  
...  
Keyword(s):  
Rural Areas ◽  
Raw Materials ◽  
Value Added ◽  
Olive Tree ◽  
Point Of View ◽  
Industrial Wastes ◽  
Olive Pomace ◽  
Crop Fields ◽  
Lignocellulosic Feedstock ◽  
Olive Stones

A biorefinery integrated process based on lignocellulosic feedstock is especially interesting in rural areas with a high density of agricultural and agro-industrial wastes, which is the case for olive crop areas and their associated industries. In the region of Andalusia, in the south of Spain, the provinces of Jaén, Córdoba and Seville accumulate more than 70% of the olive wastes generated in Spain. Therefore, the valorisation of these wastes is a matter of interest from both an environmental and a social point of view. The olive biorefinery involves a multi-product process from different raw materials: olive leaves, exhausted olive pomace, olive stones and olive tree pruning residues. Biorefinery processes associated with these wastes would allow their valorisation to produce bioenergy and high value-added renewable products. In this work, using geographic information system tools, the biomass from olive crop fields, mills and olive pomace-extracting industries, where these wastes are generated, was determined and quantified in the study area. In addition, the vulnerability of the territory was evaluated through an environmental and territorial analysis that allowed for the determination of the reception capacity of the study area. Then, information layers corresponding to the availability of the four biomass wastes, and layers corresponding to the environmental fragility of the study area were overlapped and they resulted in an overall map. This made it possible to identify the best areas for the implementation of the biorefineries based on olive-derived biomass. Finally, as an example, three zones were selected for this purpose. These locations corresponded to low fragility areas with a high availability of biomass (more than 300,000 tons/year) in a 30 km radius, which would ensure the biomass supply.

scholarly journals Improvement of Enzymatic Saccharification of Cellulose-Containing Raw Materials Using Aspergillus niger

Processes ◽  
2021 ◽  
Vol 9 (8) ◽  
pp. 1360
Author(s):  
Ekaterina Budenkova ◽  
Stanislav Sukhikh ◽  
Svetlana Ivanova ◽  
Olga Babich ◽  
Vyacheslav Dolganyuk ◽  
...  
Keyword(s):  
Enzymatic Hydrolysis ◽  
Aspergillus Niger ◽  
Filter Paper ◽  
Raw Materials ◽  
Enzymatic Saccharification ◽  
Wood Chip ◽  
Cellulase Activity ◽  
Wood Chips ◽  
Acid Pretreatment ◽  
Hydrolysis Of

Enzymatic hydrolysis of cellulose-containing raw materials, using Aspergillus niger, were studied. Filter paper, secondary cellulose-containing or starch-containing raw materials, miscanthus cellulose after alkaline or acid pretreatment, and wood chip cellulose, were used as substrates. The study focused on a wild A. niger strain, treated, or not (control), by ultraviolet (UV) irradiations for 45, 60, or 120 min (UV45, UV60, or UV120), or by UV irradiation for 120 min followed by a chemical treatment with NaN3 + ItBr for 30 min or 80 min (UV120 + CH30 or UV120 + CH80). A mixture of all the A. niger strains (MIX) was also tested. A citrate buffer, at 50 mM, wasthe most suitable for enzymatic hydrolysis. As the UV exposure time increased to 2 h, the cellulase activity of the surviving culturewas increased (r = 0.706; p < 0.05). The enzymatic activities of the obtained strains, towards miscanthus cellulose, wood chips, and filter paper, were inferior to those obtained with commercial enzymes (8.6 versus 9.1 IU), in some cases. Under stationary hydrolysis at 37 °C, pH = 4.7, the enzymatic activity of A. niger UV120 + CH30 was 24.9 IU. The enzymatic hydrolysis of secondary raw materials, using treated A. niger strains, was themost effective at 37 °C. Similarly, the most effective treatment of miscanthus cellulose and wood chips occurred at 50 °C. The maximum conversion of cellulose to glucose was observed using miscanthus cellulose (with alkaline pretreatment), and the minimum conversion was observed when using wood chips. The greatest value of cellulase activity was evidenced in the starch-containing raw materials, indicating that A. niger can ferment not only through cellulase activity, but also via an amylolytic one.

scholarly journals How Far Away Are World Economies from Circularity: Assessing the Capacity of Circular Economy Policy Packages in the Operation of Raw Materials and Industrial Wastes

2021 ◽  
Vol 13 (8) ◽  
pp. 4394
Author(s):  
Margarita Ignatyeva ◽  
Vera Yurak ◽  
Alexey Dushin ◽  
Vladimir Strovsky ◽  
Sergey Zavyalov ◽  
...  
Keyword(s):  
The Netherlands ◽  
Waste Management ◽  
South Korea ◽  
Circular Economy ◽  
Raw Materials ◽  
Industrial Wastes ◽  
New Guidelines ◽  
The 1960S ◽  
Degree Level ◽  
The Eu

Nowadays, circular economy (CE) is on the agenda, however, this concept of closed supply chains originated in the 1960s. The current growing quantity of studies in this area accounts for different discourses except the holistic one, which mixes both approaches—contextual and operating (contextual approach utilizes the thorough examination of the CE theory, stricture of the policy, etc.; the operating one uses any kind of statistical data)—to assess the capacity of circular economy regulatory policy packages (CERPP) in operating raw materials and industrial wastes. This article demonstrates new guidelines for assessing the degree level of capacity (DLC) of CERPPs in the operation of raw materials and industrial wastes by utilizing the apparatus of the fuzzy set theory. It scrupulously surveys current CERPPs in three regions: the EU overall, Finland and Russia; and assesses for eight regions—the EU overall, Finland, Russia, China, Greece, France, the Netherlands and South Korea—the DLC of CERPPs in operating raw materials and industrial wastes. The results show that EU is the best in CE policy and its CERPP is 3R. The following are South Korea and China with the same type of CERPP. Finland, France and the Netherlands have worse results than EU with the type of CERPP called “integrated waste management” because of the absence of a waste hierarchy (reduce, recover, recycle). Russia closes the list with the type of CERPP “basic waste management”.

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