Molecular investigation and phylogeny of species of the Anaplasmataceae infecting animals and ticks in Senegal

Abstract Background Our study aimed to assess the diversity of the species of Anaplasmataceae in Senegal that infect animals and ticks in three areas: near Keur Momar Sarr (northern region), Dielmo and Diop (Sine Saloum, central region of Senegal), and in Casamance (southern region of Senegal). Methods A total of 204 ticks and 433 blood samples were collected from ruminants, horses, donkeys and dogs. Ticks were identified morphologically and by molecular characterization targeting the 12S rRNA gene. Molecular characterization of species of Anaplasmataceae infecting Senegalese ticks and animals was conducted using the 23S rRNA, 16S rRNA, rpoB and groEL genes. Results Ticks were identified as Rhipicephalus evertsi evertsi (84.3%), Hyalomma rufipes (8.3%), Hyalomma impeltatum (4.9%), R. bursa (1.5%) and R. muhsamae (0.9%). The overall prevalence of Anaplasmataceae infection in ticks was 0.9%, whereas 41.1% of the sampled animals were found infected by one of the species belonging to this family. We identified the pathogen Anaplasma ovis in 55.9% of sheep, A. marginale and A. centrale in 19.4% and 8.1%, respectively, of cattle, as well as a putative new species of Anaplasmataceae. Two Anaplasma species commonly infecting ruminants were identified. Anaplasma cf. platys, closely related to A. platys was identified in 19.8% of sheep, 27.7% of goats and 22.6% of cattle, whereas a putative new species, named here provisionally “Candidatus Anaplasma africae”, was identified in 3.7% of sheep, 10.3% of goats and 8.1% of cattle. Ehrlichia canis and Anaplasma platys were identified only from dogs sampled in the Keur Momar Sarr area. Ehrlichia canis was identified in 18.8% of dogs and two R. e. evertsi ticks removed from the same sheep. Anaplasma platys was identified in 15.6% of dogs. Neither of the dogs sampled from Casamance region nor the horses and donkeys sampled from Keur Momar Sarr area were found infected by an Anaplasmataceae species. Conclusions This study presents a summary of Anaplasmataceae species that infect animals and ticks in three areas from the northern, central and southern regions of Senegal. To our knowledge, our findings demonstrate for the first time the presence of multiple Anaplasmataceae species that infect ticks and domestic animals in Senegal. We recorded two potentially new species commonly infecting ruminants named here provisionally as Anaplasma cf. platys and “Candidatus Anaplasma africae”. However, E. canis was the only species identified and amplified from ticks. None of the other Anaplasmataceae species identified in animals were identified in the tick species collected from animals.

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Detection and molecular characterization of Hepatozoon canis, Babesia vogeli, Ehrlichia canis, and Anaplasma platys in dogs from Metro Manila, Philippines

Korean Journal of Veterinary Research ◽

10.14405/kjvr.2017.57.2.79 ◽

2017 ◽

Vol 57 (2) ◽

pp. 79-88

Author(s):

Davin Edric V. Adao ◽

Charles Michael T. Herrera ◽

Luiza H. Galarion ◽

Nicole R. Bolo ◽

Rhodora S. Carlos ◽

...

Keyword(s):

Molecular Characterization ◽

Ehrlichia Canis ◽

Hepatozoon Canis ◽

Anaplasma Platys ◽

Babesia Vogeli ◽

Metro Manila

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The occurrence of pathogens in Rhipicephalus microplus ticks from cattle in Madagascar

Veterinární Medicína ◽

10.17221/59/2016-vetmed ◽

2016 ◽

Vol 61 (No. 9) ◽

pp. 516-523 ◽

Cited By ~ 4

Author(s):

A. Matysiak ◽

P. Dudko ◽

K. Dudek ◽

M. Dudek ◽

A. Junkuszew ◽

...

Keyword(s):

Strong Correlation ◽

Anaplasma Phagocytophilum ◽

Rhipicephalus Microplus ◽

Ehrlichia Canis ◽

Babesia Bovis ◽

Strong Interactions ◽

Anaplasma Ovis ◽

First Time ◽

Ehrlichia Muris

Rhipicephalus microplus is one of the most important ectoparasites of cattle in tropical and subtropical regions. In ticks collected from cattle the pathogens Babesia bovis, Anaplasma spp. and Ehrlichia spp. can be detected. Here, we report the first detection of the pathogen Anaplasma phagocytophilum in Madagascar from ticks infesting cattle. Furthermore, we report for the first time Anaplasma ovis, Ehrlichia canis, Ehrlichia ewingii and Ehrlichia muris in both R. microplus and in Madagascar. We show no correlation between the detection of B. bovis, Ehrlichia spp. and Anaplasma spp. DNA within the same tick. Previous reports have demonstrated strong interactions between A. marginale, A. centrale and A. ovis in the same tick, as well between these pathogens and A. phagocytophilum. A strong correlation also existed between the occurrences of Ehrlichia species within the same tick. Our findings suggest that R. microplus ticks are potential vectors and reservoirs of many tick-borne diseases of cattle.

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Molecular and serological detection of Ehrlichia canis in naturally exposed dogs in Iran: an analysis on associated risk factors

Revista Brasileira de Parasitologia Veterinária ◽

10.1590/s1984-29612014002 ◽

2014 ◽

Vol 23 (1) ◽

pp. 16-22 ◽

Cited By ~ 10

Author(s):

Nadi Maazi ◽

Abdolali Malmasi ◽

Parviz Shayan ◽

Seyed Mahdi Nassiri ◽

Taghi Zahraei Salehi ◽

...

Keyword(s):

Risk Factors ◽

Rural Areas ◽

Ehrlichia Canis ◽

Rrna Gene ◽

Igg Antibodies ◽

Serological Detection ◽

Blood Samples ◽

Associated Risk Factors ◽

First Time ◽

The 16S Rrna Gene

The general aim of this study, which was conducted for the first time in Iran, was to evaluate the seroprevalence and geographical distribution of Ehrlichia canis in a dog population in Iran, followed by molecular confirmation using PCR and sequencing. Blood samples were collected from 240 dogs in different areas of Alborz and Tehran Provinces and initially analyzed using the immunofluorescent antibody (IFA) test to detect anti-Ehrlichia canis IgG antibodies. Subsequently, nested PCR was performed based on a fragment of the 16S rRNA gene of E. canis on serologically positive samples. The results showed that 40/240 dogs (16.6%) presented anti-Ehrlichia canis IgG antibodies and that nine of the blood samples from the 40 seropositive dogs (22.5%) contained E. canis DNA, which was confirmed by sequencing. The seroprevalence of E. canis tended to be higher in purebred, one to three-year-old male dogs living in the Plain zone, in rural areas; however, this difference was not statistically significant.

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Characterisation of a population of Pratylenchus hippeastri from bromeliads and description of two related new species, P. floridensis n. sp. and P. parafloridensis n. sp., from grasses in Florida

Nematology ◽

10.1163/138855410x495809 ◽

2010 ◽

Vol 12 (6) ◽

pp. 847-868 ◽

Cited By ~ 13

Author(s):

Francesca De Luca ◽

Alberto Troccoli ◽

Larry W. Duncan ◽

Sergei A. Subbotin ◽

Lieven Waeyenberge ◽

...

Keyword(s):

New Species ◽

Molecular Level ◽

South Florida ◽

Rrna Gene ◽

Female Type ◽

Rdna Sequences ◽

Lesion Nematodes ◽

Type Population ◽

Root Lesion Nematodes ◽

First Time

Abstract Morphological and molecular analyses confirmed the presence of Pratylenchus hippeastri in regulatory samples collected in commercial bromeliad operations from genera Guzmania, Neoregelia and Vriesea in central and south Florida, USA. Specimens of P. hippeastri from bromeliads contained males which were not detected in the type population from amaryllis and are described herein for the first time. The rDNA sequences of these males matched those of P. hippeastri female type material. Pratylenchus hippeastri and root-lesion nematodes from several hosts in Florida were characterised at the morphological and molecular level, whereas other samples from Russia and South Africa were characterised at the molecular level only. Sequence and phylogenetic analysis using the ITS rRNA gene of these root-lesion nematodes revealed the presence of eight putative new species (spH1-H8) closely related to P. hippeastri. Here we describe two Florida representatives of the amphimictic root-lesion nematodes from Bahia grass (N1) and maidencane (N2), previously characterised by Inserra et al. in 1996 and Duncan et al. in 1999, as two new species phylogenetically related to P. hippeastri and named P. floridensis n. sp. and P. parafloridensis n. sp., respectively. The small round or oval (rarely rectangular and occasionally oblong) and enlarged spermatheca and the bluntly pointed or subacute tail with smooth and occasionally indented terminus separate P. floridensis n. sp. from P. parafloridensis n. sp., which has a quadrangular spermatheca and a subhemispherical or bluntly pointed tail with generally smooth and rarely indented terminus. However, these characters may overlap in some specimens making a morphological separation problematic without the use of molecular analysis. The close phylogenetic relationships shared by the species characterised in this study indicate that they are representatives of a P. hippeastri species complex.

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Distribution and molecular characterization of biosurfactant-producing bacteria

Biodiversitas Journal of Biological Diversity ◽

10.13057/biodiv/d210914 ◽

2020 ◽

Vol 21 (9) ◽

Author(s):

Nassir Alyousif ◽

YASIN Y.Y. AL LUAIBI ◽

WIJDAN HUSSEIN

Keyword(s):

16S Rrna ◽

16S Rrna Gene ◽

Molecular Characterization ◽

Bacillus Licheniformis ◽

Rrna Gene ◽

Bacterial Isolates ◽

Emulsification Activity ◽

Emulsification Index ◽

First Time

Abstract. Alyousif NA, Luaibi YYYA, Hussein W. 2020. Distribution and molecular characterization of biosurfactant-producing bacteria. Biodiversitas 21: 4034-4040. Biosurfactants (BSs) are biological surface-active compounds produced by several microorganisms with many areas of application, as such become an important product in biotechnology and consequence to be used in industries. In recent years, many researchers pay attention to BSs producers' microorganisms. The present study was aimed to isolate, identify, and screening BS producing bacteria from six various sites in two different cities in Iraq. Four samples were collected from four sites in Basrah governorate and the rest two samples from Al-Garraf oilfield in Thi-Qar governorate. A total of 33 different bacterial isolates were obtained, 20 out of the 33 were found to be biosurfactants producing isolates that detected through the emulsification index (E24%), oil spreading test, and emulsification activity. The isolated bacterial strains were more identified by 16S rRNA gene sequencing. The results showed that the biosurfactants producing isolates belonged to genera Bacillus, Pseudomonas, Enterobacter, Staphylococcus, Acinetobacter, and Aerococcus. Bacillus jeotgali and Aerococcus viridans are reporting as biosurfactant producing bacteria for the first time and Bacillus jeotgali is isolated for first time from crude oil of oilfield reservoir in this study in world. Moreover, six bacterial isolates were identified as new strains and deposited at NCBI Genbank under accession numbers MT261834 (Bacillus subtilis strain IRQNWYA3), MT261835 (Bacillus licheniformis strain IRQNWYB4), MT261836 (Pseudomonas stutzeri strain IRQNWYF2), MT261837 (Pseudomonas zhaodongensis strain IRQNWYF3), MT261838 (Pseudomonas sp. IRQNWYF4) and MT261839 (Bacillus licheniformis strain IRQNWYF5). A2 isolate that was identified as Pseudomonas aeruginosa has shown the highest values of emulsification activity and emulsification index (1.678±0.050 absorbance at 540 nm and 56.6% respectively) that show efficient potential of biosurfactant production. Phylogenetic tree was also constructed in this study based on 16S rRNA gene sequences of biosurfactant-producing bacteria to evaluate their close relationship and evolution between them.

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Prevalence, molecular characterization and risk factor analysis of Ehrlichia canis and Anaplasma platys in domestic dogs from Paraguay

Comparative Immunology Microbiology and Infectious Diseases ◽

10.1016/j.cimid.2018.11.015 ◽

2019 ◽

Vol 62 ◽

pp. 31-39 ◽

Cited By ~ 3

Author(s):

S. Pérez-Macchi ◽

R. Pedrozo ◽

P. Bittencourt ◽

A. Müller

Keyword(s):

Factor Analysis ◽

Risk Factor ◽

Molecular Characterization ◽

Ehrlichia Canis ◽

Domestic Dogs ◽

Risk Factor Analysis ◽

Anaplasma Platys

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Molecular Prevalence and Identification of Ehrlichia canis and Anaplasma platys from Dogs in Nay Pyi Taw Area, Myanmar

Veterinary Medicine International ◽

10.1155/2021/8827206 ◽

2021 ◽

Vol 2021 ◽

pp. 1-7

Author(s):

Myint Myint Hmoon ◽

Lat Lat Htun ◽

May June Thu ◽

Hla Myet Chel ◽

Yu Nandi Thaw ◽

...

Keyword(s):

Ehrlichia Canis ◽

Rrna Gene ◽

Limited Information ◽

Anaplasma Platys ◽

Blood Samples ◽

Glta Gene ◽

Canine Monocytic Ehrlichiosis ◽

Veterinary Importance ◽

Healthy Dogs

Ticks are vectors of different types of viruses, protozoans, and other microorganisms, which include Gram-negative prokaryotes of the genera Rickettsiales, Ehrlichia, Anaplasma, and Borrelia. Canine monocytic ehrlichiosis caused by Ehrlichia canis and canine cyclic thrombocytopenia caused by Anaplasma platys are of veterinary importance worldwide. In Myanmar, there is limited information concerning tick-borne pathogens, Ehrlichia and Anaplasma spp., as well as genetic characterization of these species. We performed nested PCR for the gltA gene of the genus Ehrlichia spp. and the 16S rRNA gene of the genus Anaplasma spp. with blood samples from 400 apparently healthy dogs in Nay Pyi Taw area. These amplicon sequences were compared with other sequences from GenBank. Among the 400 blood samples from dogs, 3 (0.75%) were positive for E. canis and 1 (0.25%) was positive for A. platys. The partial sequences of the E. canis gltA and A. platys 16SrRNA genes obtained were highly similar to E. canis and A. platys isolated from different other countries.

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First study of Cryptosporidium spp. occurrence in eared doves (Zenaida auriculata)

Revista Brasileira de Parasitologia Veterinária ◽

10.1590/s1984-29612019016 ◽

2019 ◽

Vol 28 (3) ◽

pp. 489-492

Author(s):

Mércia de Seixas ◽

Alessandra Taroda ◽

Sérgio Tosi Cardim ◽

João Pedro Sasse ◽

Thais Agostinho Martins ◽

...

Keyword(s):

Molecular Characterization ◽

Protozoan Parasite ◽

18S Rrna Gene ◽

Pcr Analysis ◽

Rrna Gene ◽

Cryptosporidium Spp ◽

Potential Source ◽

Wide Range ◽

First Time

Abstract Cryptosporidium is a protozoan parasite with a wide range of hosts, including humans. However, only a few Cryptosporidium species have been described in birds (C. meleagridis, C. baileyi, C. galli and C. avium). The aim of this study was to investigate the occurrence of Cryptosporidium spp. in feces of eared doves (Zenaida auriculata), followed by molecular characterization of the parasite. A total of 196 animals of both sexes were trap-captured; the animals were culled and the intestinal contents were collected for DNA extraction. After extraction, a nested-PCR (nPCR), which amplifies a fragment of the 18S rRNA gene of Cryptosporidium spp., was performed. The amplicons obtained were purified and sequenced. PCR analysis revealed that 30 animals (15.3%) were positive for Cryptosporidium spp. There was no significant sex-dependent enrichment of Cryptosporidium occurrence (p > 0.05). Only 15 out of the 30 positive samples were successfully sequenced and their species determined, of which, 13 (86.7%) and 2 (13.3%) were C. meleagridis and C. galli, respectively. Herein, we present for the first time a molecular characterization of Cryptosporidium from feces of eared doves (Z. auriculata) and propose that these birds are a potential source of C. meleagridis infection in humans.

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Identification and molecular characterization of Chryseobacterium vrystaatense ST1 isolated from oligomineral water of southeast Serbia

Archives of Biological Sciences ◽

10.2298/abs1203877t ◽

2012 ◽

Vol 64 (3) ◽

pp. 877-883

Author(s):

S. Tasic ◽

M. Kojic ◽

S. Stankovic ◽

D. Obradovic

Keyword(s):

16S Rrna ◽

16S Rrna Gene ◽

Molecular Characterization ◽

Universal Primers ◽

Rrna Gene ◽

Bacterial Strains ◽

Biochemical Tests ◽

First Time

The isolation and molecular characterization of bacterial strains isolated from water sources in the Vlasina Mountain in southeast Serbia, confirmed the presence of a new species Chryseobacterium vrystaatense ST1. This Gram- negative species showed an extremely low level of biochemical reactivity in biochemical tests. The gene for 16S rRNA was amplified by PCR using universal primers and sequenced. Comparison of 16S rRNA gene sequence and phenotypic features indicated that the isolate ST belonged to Chryseobacterium vrystaatense. A BLAST search of sequenced 1088 nucleotides of the 16S rRNA gene with all sequences deposited in the NCBI collection showed the highest similarity (98%) with the strain Chryseobacterium vrystaatense sp. nov., designated as strain R-23533. The very high homology of these two strains allowed classification of our strain at the species level, but some differences indicate, and indirectly confirm, that the isolate ST is an authentic representative. On the basis of these results, we could conclude that Chryseobacterium vrystaatense ST was for first time isolated in Serbia, which is particularly important when one bears in mind that there are only three sequences of this species deposited in the NCBI collection.

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