Geographic distribution of the cagA, vacA, iceA, oipA and dupA genes of Helicobacter pylori strains isolated in China

Abstract Background There are geographic variations in the genotypes of Helicobacter pylori (H. pylori) cagA, vacA, iceA, oipA and dupA. The aim of the study was to investigate the distribution of these genotypes among H. pylori strains from five regions of China and their association with clinical outcomes. Materials and methods Gastric biopsy specimens were obtained from 348 patients with different gastrointestinal diseases in the five regions of China. The regional distribution was 89 patients from Shandong, 91 from Guangxi, 57 from Hunan, 58 from Qinghai and 53 from Heilongjiang. The presence of cagA, vacA, iceA, oipA and dupA genotypes was determined by polymerase chain reaction (PCR) from H. pylori DNA. Results A total of 269 H. pylori isolates were obtained, of which 74 isolates were from Shandong, 78 from Guangxi, 46 from Hunan, 33 from Qinghai and 38 from Heilongjiang. The cagA-positive status was predominant in the five regions. The predominant vacA genotypes were s1c (73.4%), m2 (70.6%) and i1 (92.9%). In strains from Shandong, s1a and m1 were dominant. By contrast, s1c was dominant in Guangxi and i1 was dominant in Hunan and Heilongjiang. The prevalence of m2 subtype in Qinghai (78.8%) was significantly higher than that in other regions (P < 0.05). The predominant iceA genotype was iceA1 and the frequency of iceA1 was significantly more prevalent in Hunan than in other regions (P < 0.05). The oipA status “on” gene was more frequent in Shandong (91.9%) and Guangxi (91%) than in Heilongjiang (71.7%) (P < 0.05). Conversely, the dupA-positive status was less than half in Shandong (31.1%) and Guangxi (15.4%), whereas it was 73.9% in Hunan and 81.8% in Qinghai (P < 0.001). There were no significant associations between the cagA, vacA, iceA, oipA genotypes and clinical outcomes. The dupA-positive strains were more common in peptic ulcer disease (PUD) patients than in non-ulcer dyspepsia (NUD) patients in Shandong and Guangxi (P < 0.05), but the association was not observed in other geographic regions. Conclusions There was significant geographic diversity of H. pylori genotypes in different regions of China and the presence of dupA gene can be considered as a marker for the development of gastroduodenal diseases. However, the cagA, iceA, vacA and oipA genes cannot be regarded for prediction of the clinical presentation of H. pylori infection in China.

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Analysis of Helicobacter pylori Genotypes in Afghani and Iranian Isolates

Polish Journal of Microbiology ◽

10.33073/pjm-2010-009 ◽

2010 ◽

Vol 59 (1) ◽

pp. 61-66 ◽

Cited By ~ 8

Author(s):

HOSSEIN DABIRI ◽

MEHDI BOLFION ◽

AKBAR MIRSALEHIAN ◽

MARYAM REZADEHBASHI ◽

FERESHTEH JAFARI ◽

...

Keyword(s):

Helicobacter Pylori ◽

Peptic Ulcer ◽

Clinical Outcomes ◽

Positive Culture ◽

Chain Reaction ◽

Vacuolating Cytotoxin ◽

Ulcer Disease ◽

Polymerase Chain ◽

H Pylori ◽

Iranian Patients

The geographical variation in Helicobacter pylori genotypes is an observed phenomenon. Cytotoxin associated genes A (cagA) and E (cagE), and vacuolating cytotoxin (vacA) genotypes of H. pylori are associated with peptic ulcer disease (PUD). This study compared the distribution of these genotypes in Iranian and Afghani isolates and their association with clinical outcomes. H. pylori infected patients, as proven by positive culture, were recruited prospectively. A total of 70 patients, 55 Iranian (26 men and 29 women, mean age 48 +/- 18 years) and 15 Afghani immigrants (13 men and 2 women, mean age 34.8 +/- 11 years) living in Tehran, Iran were enrolled in this study. DNA was extracted from isolated H. pylori and polymerase chain reaction was carried out to determine the cagA and cagE status and vacA alleles. The number of gastric cancer, peptic ulcer and gastritis cases was 11, 23 and 36, respectively. The cagA positive isolates were more common in Iranian (67%) than Afghani isolates (60%). cagE was positive in 53% of Afghani compared to 51% of Iranian isolates. The most common vacA s-region genotype was s1; 80% in Afghani and 67% in Iranian. The slml was a frequently observed genotype in Afghani strains (53%) while s1m2 (47%) was more common in strains isolated from Iranian patients. There is a difference in the H. pylori strains between Iranian and Afghani groups, for instance Iranian isolates were similar to European isolates while Afghani isolates were similar to isolates from India. However, there was no significant association between cagA, cagE and vacA genotypes and clinical outcomes in Iranian and Afghani patients.

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Phenotypic and Genotypic Analysis of Resistant Helicobacter pylori Strains Isolated from Children with Gastrointestinal Diseases

Diagnostics ◽

10.3390/diagnostics10100759 ◽

2020 ◽

Vol 10 (10) ◽

pp. 759

Author(s):

Monika Maria Biernat ◽

Aldona Bińkowska ◽

Łukasz Łaczmański ◽

Paweł Biernat ◽

Paweł Krzyżek ◽

...

Keyword(s):

Helicobacter Pylori ◽

Antibiotic Resistance ◽

Point Mutations ◽

Gastrointestinal Diseases ◽

Drug Susceptibility Testing ◽

Test Method ◽

Ulcer Disease ◽

Genotypic Analysis ◽

Clinical Diagnoses ◽

H Pylori

Antibiotic resistance of Helicobacter pylori is currently a global issue. The aim of this study was to analyze actual antibiotic resistance rates of H. pylori strains isolated from children with primary infections and to compare the incidence of mutations that determine resistance to clarithromycin (CH) and metronidazole (MET) in children with different clinical diagnoses. A total of 91 H. pylori strains were isolated from 108 children with primary infections. Drug susceptibility testing of the strains was performed using E-test method. Classical sequencing of DNA fragments was used to detect point mutations for CH and MET resistance. Resistance to CH was detected in 31% of isolated strains (28/91), while resistance to MET and CH was detected in 35% (32/91) of strains. A2143G was the most frequently detected mutation and was dominant among strains isolated from children with peptic ulcer disease (80%). Mutations in the rdxA gene were found significantly more frequently among MET-resistant strains than MET-sensitive strains (p = 0.03, Chi2 = 4.3909). In children, a higher frequency of H. pylori multiresistant strains was observed compared with the previous study in the same area. Differences were found in the occurrence of point mutations among H. pylori strains resistant to CH isolated from children with different clinical diagnoses.

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Analysis of Helicobacter pylori Prevalence in Chittagong, Bangladesh, Based on PCR and CLO Test

Microbiology Insights ◽

10.4137/mbi.s39858 ◽

2016 ◽

Vol 9 ◽

pp. MBI.S39858 ◽

Cited By ~ 2

Author(s):

Abdul Musaweer Habib ◽

Jibran Alam ◽

Bashudev Rudra ◽

Abdul Quader ◽

Mohammad Al-Forkan

Keyword(s):

Helicobacter Pylori ◽

Current Trend ◽

Chain Reaction ◽

Gastric Diseases ◽

Age And Gender ◽

Ulcer Disease ◽

Specific Polymerase Chain Reaction ◽

Polymerase Chain ◽

And Gender ◽

H Pylori

The pathogenic bacterium Helicobacter pylori is a causative agent of gastric diseases in Bangladesh as well as throughout the world. This study aimed at analyzing the prevalence of H. pylori infection among dyspeptic patients in Chittagong, the second most populous city of Bangladesh, using 16S rRNA-based H. pylori-specific Polymerase Chain Reaction and Campylobacter-like organism test. We found that 67% of the population under study was positive for H. pylori infection. Gastric ulcer and duodenal ulcer disease showed statistically significant association with H. pylori infection; however, no association of H. pylori infection was observed in terms of age and gender. This study would play a crucial role in managing H. pylori-induced gastric diseases by understanding the current trend of H. pylori infection in the Chittagong region of Bangladesh.

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Clinical outcome after infection with Helicobacter pylori does not appear to be reliably predicted by the presence of any of the genes of the cag pathogenicity island

Gut ◽

10.1136/gut.43.6.752 ◽

1998 ◽

Vol 43 (6) ◽

pp. 752-758 ◽

Cited By ~ 75

Author(s):

P J Jenks ◽

F Mégraud ◽

A Labigne

Keyword(s):

Duodenal Ulcer ◽

Helicobacter Pylori ◽

Clinical Outcome ◽

Insertion Sequence ◽

Pathogenicity Island ◽

Polymerase Chain ◽

Non Ulcer Dyspepsia ◽

H Pylori ◽

Outcome After Infection

Background—The development of clinical disease after infection with Helicobacter pylori has been reported to be associated with expression of the cagA gene. Recently, it has been shown that cagA is part of a multigene locus, described as the cag pathogenicity island (PAI). The role of this region in determining clinical outcome remains to be established.Aims—To investigate whether the presence ofcagA is always associated with the presence of the complete cag PAI and to evaluate the distribution of selected cag genes in 73 H pylori strains isolated from patients in France.Methods—Clinical strains of H pyloriwere screened for selected genes of the cag PAI by polymerase chain reaction and colony hybridisation.Results—Of 64 strains that harboured thecagA gene, 57 (89%) also contained the entirecag PAI. The entire cag PAI was found in 85% (48/56) and 53% (9/17) of duodenal ulcer and non-ulcer dyspepsia isolates, respectively. Eight strains had deletions within thecag PAI, including deletion of the cagA gene in one isolate; the deletions were not associated with the insertion sequence IS605. Of eight strains lacking the cag PAI, four were isolated from patients with duodenal ulcer.Conclusion—The cag PAI is not a uniform, conserved entity. Although the presence of thecag PAI is highly associated with duodenal ulcer, the clinical outcome of infection with H pylori is not reliably predicted by any gene of the cag PAI.

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Geographic Distribution of The cagA, vacA, iceA, oipA and dupA Genes of Helicobacter Pylori Strains Isolated in China

10.21203/rs.3.rs-136079/v1 ◽

2020 ◽

Author(s):

Zhijing Xue ◽

Hong Yang ◽

Dongxing Su ◽

Xiangfeng Song ◽

Xin Deng ◽

...

Keyword(s):

Helicobacter Pylori ◽

Regional Distribution ◽

Chain Reaction ◽

Geographical Differences ◽

Biopsy Specimens ◽

Geographic Differences ◽

Polymerase Chain ◽

The Difference ◽

H Pylori ◽

Geographical Characteristics

Abstract Background: There were geographical differences in the distribution of Helicobacter pylori (H. pylori) genotypes (cagA, vacA, iceA, oipA and dupA, et al). The population in different regions in China have grant different patterns of gastroduodenal diseases which are associated with these genotypes, but the geographical characteristics of H. pylori genotypes were still unknown. Materials and Methods: Gastric biopsy specimens were obtained from 348 patients from five regions in China. The regional distribution was 89 patients from Shandong, 91 from Guangxi, 57 from Hunan, 58 from Qinghai and 53 from Heilongjiang. DNA extracted from cultured isolates were analyzed by polymerase chain reaction (PCR) to determine the presence of cagA, vacA, iceA, oipA and dupA genotypes.Results: A total of 269 H. pylori isolates were obtained, of which 74 isolates were from Shandong, 78 from Guangxi, 46 from Hunan, 33 from Qinghai and 38 from Heilongjiang. The cagA gene was predominant in all the five regions (e.g. 100% in Hunan, Qinghai and Heilongjiang). The predominant vacA genotypes in the 269 isolates were s1a (88.1%) and m1(72.1%). vacA s1b was not detected in our study. In strains from Guangxi and Hunan, s1c was dominant; in contrast, s1a was dominant in Shandong, Qinghai and Heilongjiang. The prevalence of m1 strains in Heilongjiang (92.1%) was significantly higher (P<0.001) than in Shandong (60.8%) and Qinghai (51.5%). The dominant vacA subtype combination was s1a/m1 (62.8%) and detection of vacA s1a/m1 was significantly high 34 (89.5%) in Heilongjiang strains (P<0.001). The prevalence of iceA alleles in Hunan and Qinghai was much higher than that in the other three regions, and the difference was statistically significant. The oipA-positive strains were more prevalent in Guangxi (100%) and Hunan (100%) than in Qinghai (78.8%) (P<0.001). Conversely, the dupA-positive strains were less than half in Guangxi (15.4%) and Shandong (32.4%), whereas it was 73.9% in Hunan and 81.8% in Qinghai (P<0.001).Conclusions: There are significant geographic differences in the distribution of H. pylori genotypes. These datas may be used to explain the gastroduodenal diseases patterns in different geographic regions of China.

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Serum Helicobacter pylori Specific Antibodies Among Children With Undernourishment: A Case Control Study

Bangladesh Journal of Microbiology ◽

10.3329/bjm.v28i1.11805 ◽

2012 ◽

Vol 28 (1) ◽

pp. 25-31

Author(s):

Warda Haque ◽

Chowdhury Rafiqul Ahsan ◽

Jamalun Nessa ◽

Mahmuda Yasmin

Keyword(s):

Helicobacter Pylori ◽

Drinking Water ◽

Nutritional Status ◽

Breast Feeding ◽

Poverty Reduction ◽

Feeding Practice ◽

Ulcer Disease ◽

Non Ulcer Dyspepsia ◽

Immunoblot Technique ◽

H Pylori

Infection with Helicobacter pylori has been implicated in the development of acute and chronic gastritis, peptic ulcer disease, non-ulcer dyspepsia, and gastric MALT (mucosa-associated lymphoid tissue) lymphoma and extragastroduodenal disorders. H. pylori infection may lead to development of hypochlorhydria and subsequent malnourishment. This study examined the affiliation between H. pylori infection and some sociodemographic and nutritional variables including breast feeding practice, poverty, and availability of safe drinking water. Three hundred children with age ranging from zero to twelve years were enrolled in the study. Their nutritional status was assessed following standard protocol. A precoded questionnaire was filled up for obtaining data. Serostatus of anti H. pylori antibody was determined by in-house ELISA using formalin fixed H. pylori whole cell antigen. The cut-off value of the ELISA was further validated by Immunoblot technique. Among the 149 case (malnourished) children, 107 (72%) contained anti-H. pylori antibodies and among the control (nonmalnourished) population (n=151), 91 (60%) possessed anti-H. pylori antibodies, with an overall seroprevalence of 66%. Malnourishment was found to be more widespread among female (56%) than male children (44%). Highly significant association (p = 0.000) was reported between poor socioeconomic status and development of malnourishment. The degree of severity of malnourishment increased inversely with the practice of exclusive breast-feeding up to 5 months of age (p = 0.010). The odds ratio relating breastfeeding to malnourishment reflected 52% reduction in the risk of development of malnourishment among breast-fed children. The use of unsafe drinking water was associated with 5.33 times higher odds of developing malnourishment. Poverty reduction strategies associated with improvement of hygiene condition and promotion of breast feeding can actively contribute to the improvement of the nutritional status of the children of Bangladesh. DOI: http://dx.doi.org/10.3329/bjm.v28i1.11805 Bangladesh J Microbiol, Volume 28, Number 1, June 2011, pp 25-31

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Molecular Identification of Helicobacter pylori and IceA Genes Frequency from Dental Plaques Isolated from People Using PCR Method

International Journal of Medical Laboratory ◽

10.18502/ijml.v7i3.4137 ◽

2020 ◽

Author(s):

Zahra Salari ◽

Atefeh Ranjkesh ◽

Emad Behboudi

Keyword(s):

Polymerase Chain Reaction ◽

Helicobacter Pylori ◽

Chain Reaction ◽

Ulcer Disease ◽

Two Samples ◽

Pcr Method ◽

Dental Plaques ◽

Polymerase Chain ◽

H Pylori ◽

Development And Evolution

Background and Aims: Helicobacter pylori (H. pylori) is a gram-negative, microaerophilic, spiral-shaped flagellated bacterium that is urease, catalase and oxidase positive. One of its pathogenicity factors is the iceA gene. H. pylori has recently been recognized as a genetic indicator for the development and evolution of duodenal ulcer disease in the East. This study aimed to determine the presence of this bacterium in gingival plaques in non-endocrine patients in Bojnourd city, and the polymerase chain reaction technique examined the percentage of iceA gene. Materials and Methods: A total of 100 samples of dental plaque were taken and transferred to a tube that has been physiologically placed. After DNA extraction, primer design was performed, and then the polymerase chain reaction was performed for the whole sample. Results: Of 100 samples examined in this study, two samples of H. pylori were positive (2%), and the frequency of the iceA gene of two samples was positive (100%). Conclusion: In the Bojnord city, the frequency of iceA gene in people is high, and the frequency of H. pylori in tooth plaques is low. Also, iceA gene can be considered as an indicator for predicting the contamination and risk of H. pylori infection in the region. To confirm the results, more molecular studies are required in other populations.

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HELICOBACTER PYLORI cagA VIRULENCE GENE AND SEVERE ESOGASTRODUODENAL DISEASES: IS THERE AN ASSOCIATION?

Arquivos de Gastroenterologia ◽

10.1590/s0004-2803.202100000-85 ◽

2021 ◽

Vol 58 (4) ◽

pp. 468-475

Author(s):

Ana Karoline Silva OLIVEIRA ◽

Lucas Luiz de Lima SILVA ◽

Marina Pacheco MIGUEL ◽

Angel José Vieira BLANCO ◽

Lilian Carla CARNEIRO ◽

...

Keyword(s):

Polymerase Chain Reaction ◽

Helicobacter Pylori ◽

Clinical Outcomes ◽

Gastric Adenocarcinoma ◽

Gastric Lymphoma ◽

Caga Gene ◽

Chain Reaction ◽

Increased Risk ◽

Polymerase Chain ◽

H Pylori

ABSTRACT BACKGROUND: Helicobacter pylori colonizes approximately half of the world’s human population. Its presence in the gastric mucosa is associated with an increased risk of gastric adenocarcinoma, gastric lymphoma, and peptic ulcer disease. In Brazil, the high prevalence of H. pylori infection is a serious health problem. H. pylori virulence factors are associated with an increased risk of serious gastrointestinal disorders. The cagA gene encodes a cytotoxin-A-associated antigen (CagA) that is involved in bacterial pathogenicity. H. pylori strains carrying the cag pathogenicity island (cag-PAI) are significantly associated with severe clinical outcomes and histopathological changes. OBJECTIVE: The present study aims to investigate the prevalence of the cagA gene among H. pylori isolates from patients with different gastric pathologies. Further, the study hopes to verify its association with clinical outcomes. In addition, phylogenetic analysis was performed on cagA-positive H. pylori strains from patients with severe and non-severe diseases. METHODS: Gastric specimens were collected through a biopsy from 117 patients with different esogastroduodenal diseases. DNA was extracted from these gastric specimens and the polymerase chain reaction was performed to amplify the gene fragments corresponding to the 16S ribosomal RNA and cagA genes using specific primers. The polymerase chain reaction products of selected samples positive for cagA were sequenced. The sequences were aligned with reference sequences from the National Center for Biotechnology Information (NCBI) (Bethesda/USA), and a phylogenetic tree was constructed. RESULTS: H. pylori was detected in 65.9% (77/117) of Brazilian patients with different gastroduodenal disorders. Overall, 80.5% (62/77) of the strains were cagA-positive. The ages of patients with cagA-positive strains (15 males and 47 females) ranged from 18 to 74 years. The lesions were categorized as non-severe and severe according to the endoscopic and histopathological reports the most prevalent non-severe esogastroduodenal lesion was gastritis 54/77 (70.12%), followed by esophagitis 12/77 (15.58%) and duodenitis 12/77 (15.58%). In contrast, the most prevalent severe lesions were atrophy 7/77 (9.09%), followed by metaplasia 3/77 (3.86%) and gastric adenocarcinoma 2/77 (2.59%). Phylogenetic analyses performed with the partial sequences of the cagA gene obtained from local strains were grouped in the same clade. No differences in phylogenetic distribution was detected between severe and non-severe diseases. CONCLUSION: The cagA gene is highly prevalent among H. pylori isolates from gastric lesions in Brazilian patients. The presence of the cagA gene was not considered a marker of the severity of esogastroduodenal lesions in the present study. This is the first study to investigate the phylogenetic population structure of H. pylori strains in a Brazilian capital, which may improve our understanding of the clinical outcome of H. pylori infection.

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Analysis of babA, cagE and cagA Genes in Helicobacter pylori from Upper Gastric Patients in the North of Iran

Infectious Disorders - Drug Targets ◽

10.2174/1871526518666180515113218 ◽

2019 ◽

Vol 19 (3) ◽

pp. 274-278 ◽

Cited By ~ 1

Author(s):

Saba Fakhrieh Asl ◽

Mehrnaz Pourvahedi ◽

Ali Mojtahedi ◽

Mohammad Shenagari

Keyword(s):

Helicobacter Pylori ◽

Gastric Diseases ◽

Specific Primers ◽

The North ◽

Different Types ◽

North Of Iran ◽

Gastric Biopsies ◽

Pcr Method ◽

Non Ulcer Dyspepsia ◽

H Pylori

Objective:Helicobacter pylori is a Gram-negative bacterium which has a serious effect on up to half of the world’s population and has been related to different gastric diseases. The goal of this study was to assess the frequency of babA, cagE and cagA genotypes among H. pylori strains isolated from gastric biopsies of endoscopic patients in the north of Iran.Methods:The present study was performed on 90 strains of H. pylori isolated from patients with gastric diseases (Gastric ulcer (GU), Duodenal ulcer (DU), Gastritis (G), Non-ulcer dyspepsia (NUD) and Gastric adenocarcinoma (GC)). DNA was extracted from all isolated strains and PCR method was performed to detect the prevalence of babA2, cagE and cagA genes using specific primers.Results:Among 90 samples of H. pylori, babA2, cagE, and cagA genes were detected in 42.2%, 30% and 82.2% of strains respectively. The statistical analysis showed that the prevalence of cagA gene in GU, G, DU, and NUD was significantly higher than other genes. Moreover, cagA, and babA2 genes were significantly more prevalent in GC patients compared to cagE gene. Our isolates exhibited 8 distinct arrangements of virulence patterns. The occurrence of cagA (35.6%) was the most prevalent pattern followed by cagA/babA2 (20%) and cagA/babA2/cagE (14.4%).Conclusion:In summary, as first report from Guilan province in the north of Iran, we showed significant association between the presence of babA2, cagE, and cagA genes in different types of gastric disorders.

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The correlation between lncRNAs and Helicobacter pylori in gastric cancer

Pathogens and Disease ◽

10.1093/femspd/ftaa004 ◽

2019 ◽

Vol 77 (9) ◽

Author(s):

Narges Dastmalchi ◽

Seyed Mahdi Banan Khojasteh ◽

Mirsaed Miri Nargesi ◽

Reza Safaralizadeh

Keyword(s):

Gastric Cancer ◽

Helicobacter Pylori ◽

Molecular Mechanisms ◽

Gastrointestinal Diseases ◽

Mechanisms Of Action ◽

Molecular Pathways ◽

Gastric Diseases ◽

Non Coding Rnas ◽

H Pylori ◽

The Relationship

ABSTRACT Helicobacter pylori infection performs a key role in gastric tumorigenesis. Long non-coding RNAs (lncRNAs) have demonstrated a great potential to be regarded as effective malignancy biomarkers for various gastrointestinal diseases including gastric cancer (GC). The present review highlights the relationship between lncRNAs and H. pylori in GC. Several studies have examined not only the involvement of lncRNAs in H. pylori-associated GC progression but also their molecular mechanisms of action. Among the pertinent studies, some have addressed the effects of H. pylori infection on modulatory networks of lncRNAs, while others have evaluated the effects of changes in the expression level of lncRNAs in H. pylori-associated gastric diseases, especially GC. The relationship between lncRNAs and H. pylori was found to be modulated by various molecular pathways.

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