Methods for the Detection of Helminth Eggs in Stool Samples

2006 ◽  
pp. 341-345
Keyword(s):  
Helminth Eggs ◽  
Stool Samples

scholarly journals Diversity and prevalence of gastrointestinal parasites with zoonotic potential of Green Monkeys in Bandia Reserve in Senegal

2021 ◽  
Vol 7 (1) ◽  
pp. 65-69
Author(s):  
Kacou Martial N'da ◽  
Laibané Dieudonné Dahourou ◽  
Oubri Bassa Gbati ◽  
Rianatou Bada Alambedji
Keyword(s):  
Staining Technique ◽  
Gastrointestinal Parasites ◽  
Human Populations ◽  
Zoonotic Potential ◽  
Gastrointestinal Parasite ◽  
Chlorocebus Aethiops ◽  
Helminth Eggs ◽  
Stool Samples ◽  
Zoonotic Parasites ◽  
Green Monkeys

Background and Aim: Parasitic and infectious diseases are ubiquitous threats to primate and human populations. This study was carried out to study the diversity and frequency of gastrointestinal parasites with zoonotic potential in green monkeys in the Bandia Reserve located in Senegal. Materials and Methods: For this study, 164 stool samples of Chlorocebus aethiops sabaeus (green monkey) were collected from October to November 2018 from Bandia Reserve. Stool samples were processed using the merthiolate-iodine-formalin staining technique and the modified Ritchie method. The slides were examined under a microscope and the identification of parasites was based on the morphology of protozoan cysts and helminth eggs. The analysis of data was conducted using R version 3.4.3 with p=0.05. Results: A total of six species of parasites were found, including five protozoa (Entamoeba coli, Entamoeba histolytica/ dispar, Entamoeba hartmanni, Endolimax nana, and Iodamoeba butschlii) and one nematode (Strongyloides spp.). The overall prevalence of gastrointestinal parasite was 76.2% (95% CI: 69.7%-82.7%). Two parasites with zoonotic potential, mainly E. histolytica/dispar with a prevalence of 13.4% (95% CI: 8.2%-18.6%) and Strongyloides spp. with a prevalence of 6.7% (95% CI: 2.9%-10.5%), were found. Conclusion: This study indicated that the monkeys of the Bandia Reserve are infested by zoonotic parasites and can, therefore, ensure transmission to visitors to the Reserve. The parasitological data that we report are the first available for these species of monkeys in the Bandia Reserve.

scholarly journals Helminth Associated Infections Among Residents of Karachi

2020 ◽  
Vol 11 (2) ◽  
pp. 96-100
Author(s):  
Aly Khan ◽  
Nasira Khatoon ◽  
S. Shahid Shaukat ◽  
Samina Waheed ◽  
Adnan Khan ◽  
...  
Keyword(s):  
Age Groups ◽  
Developed Countries ◽  
Gastrointestinal Parasites ◽  
Ethical Review ◽  
Chi Square ◽  
Male And Female ◽  
Helminth Eggs ◽  
Chi Square Test ◽  
Inflammatory Bowel ◽  
Stool Samples

Background: Parasitic Helminth parasite affects approximately 2 million individuals yearly throughout the world. These infections are extremely important while often receive inadequate consideration. Helminths are among the gastrointestinal parasites mainly involved in an increased ratio of inflammatory bowel disease, especially in underdeveloped and developed countries. Objectives: To study the prevalence of helminth eggs in stool samples collected from five hospitals of Gulistan-e-Johar area of Karachi, Sindh among different age groups of male and female patients, the targeted age groups were < 1-14, 15-40 and > 40 yrs. Methodology: All the samples included in the study, were collected according to ethical review board policy of the participating hospital. Stool Samples were obtained from a total of 1042 patients, of which 198 samples were found to be positive for helminth eggs. The helminths included were Ascaris lumbricoides, Ancylostoma duodenales and Hymenolepis nana. The association between helminth infection (A. lumbricoides, A. duodenale and H. nana) and age group was found to be significant. Results: Data was structured into male and female, and the association between sex and the intensity of infection gave highly significant results based on the chi-square test. Males showed a noticeable prevalence of helminths than females. Single, double and triple infections were also recorded. Conclusion: Present study could be very beneficial for developing programs not only for awareness in the urban and rural population but also for the control of infections due to helminths in Pakistan.

scholarly journals Effect of Incorporation of Bead-Beating during DNA Extraction for Detection of Trichuris trichiura in Stool Samples in Community Settings: A Systematic Review

2021 ◽  
Author(s):  
Divya Rana ◽  
Nischal Pokhrel
Keyword(s):  
Dna Extraction ◽  
Odds Ratio ◽  
Quantitative Polymerase Chain Reaction ◽  
Risk Of Bias ◽  
Community Settings ◽  
Real Patient ◽  
Bead Beating ◽  
Helminth Eggs ◽  
Stool Samples ◽  
Google Search

Objectives: This meta-analysis was designed to assess the effect of addition of a bead-beating step during DNA extraction to effectively isolate Trichuris trichura DNA for quantitative Polymerase Chain Reaction (qPCR)-based diagnosis. Abstract was reported according to PRISMA-DTA abstract checklist. Methods: Eligibility criteria: qPCR-based molecular studies comparing the inclusion of bead-beating step during the DNA extraction from stool samples with extraction without the step were included in the analysis. Information sources: Studies using real patient samples in community settings were included. PubMed and Google search engine were searched in December 2019. Risk of bias and applicability: Risk of bias and applicability were assessed using QUADAS-2 checklist. Synthesis of results: Odds ratio for individual studies were combined to estimate Random Effects Model odds ratio. Additional literature were searched to discuss biochemical nature of helminth eggs. Results:Included studies: A total of six independent sub-studies were gathered from two published original articles. Division of the two major studies into six sub-studies was indispensable due to natures of the study carried. 128 of total 192 samples (in all studies) were positive for Trichiuris trichiura when bead-beating was used during DNA extraction compared to 108/192 when bead-beating was excluded. Combined odds ratio was 1.66 (95% CI: 1.059 to 2.602). Biochemical nature of helminth eggs was discussed. Discussions: Strengths and limitations: Though only two article were included in the study, six exclusive individual sub-studies were analyzed. Inherent differences in the background prevalence of helminth in study population could impact sensitivity of qPCR. Interpretation: It was found that the inclusion of the bead-beating step during DNA extraction significantly increased the sensitivity of the test.

C. difficile, Other Pathogens Often in ED Stool Samples

2006 ◽  
Vol 40 (11) ◽  
pp. 17
Author(s):  
JOHN R. BELL
Keyword(s):  
Stool Samples

Rapid detection of Rotavirus antigen in stool sample of acute diarrheic children

2012 ◽  
Vol 6 (1) ◽  
pp. 11-13
Author(s):  
Sushmita Roy ◽  
S.M. Shamsuzzaman ◽  
K.Z. Mamun
Keyword(s):  
Stool Sample ◽  
Rapid Detection ◽  
Cross Sectional Study ◽  
Rapid Diagnosis ◽  
Age Group ◽  
College Hospital ◽  
Cross Sectional ◽  
Rotavirus Infection ◽  
Medical College ◽  
Stool Samples

Rotavirus is one of the leading causes of pediatric diarrhea globally. Accurate and rapid diagnosis of Rotavirus diarrhea should reduce unnecessary use of antibiotics and ultimately reduce drug resistance. Study was designed for rapid diagnosis of Rotavirus antigen in stool sample by ICT (Immunochromatographic test) as well as to observe the seasonal variation of rotavirus infection. This cross sectional study was carried out in the department of Microbiology, Dhaka Medical College from January 2011 to December 2011. Eighty stool samples were collected from Dhaka Shishu Hospital and Dhaka Medical College Hospital. All samples were tested for rotavirus antigen by ICT. Among 80 patients, 42 (52.5%) samples were positive for rotavirus antigen. Among these 42 positive samples, 30 (71.43%) were from 0-12 months of age group, 10 (23.81%) from 13 to 24 months of age group and rest 2 (4.76%) from 25 to 36 months of age group. Rotavirus Ag was detected in stool samples from January to April and another peak episode from October to December. Considering the importance of Rotavirus associated diarrhea, rapid detection of Rotavirus infection in human is substantially needed and should be routinely practiced.DOI: http://dx.doi.org/10.3329/bjmm.v6i1.19354 Bangladesh J Med Microbiol 2012; 06(01): 11-13

An Epidemiological, Diagnostic and Therapeutic Study of Giardia lamblia in Anbar Province – Iraq

2019 ◽  
Vol 9 (01) ◽  
pp. 39-45
Author(s):  
Suad Shallal Shahatha
Keyword(s):  
Teaching Hospital ◽  
Rural Areas ◽  
Giardia Lamblia ◽  
Urban Areas ◽  
Culture Media ◽  
Age Groups ◽  
Stool Samples ◽  
Women And Children ◽  
Teucrium Polium L ◽  
Therapeutic Study

This study was carried out to investigate the epidemiology of Giardia lamblia parasites in patients who visited some of the hospitals in Anbar province, which included (Fallujah Teaching Hospital, Ramadi Teaching Hospital, Ramadi Teaching Hospital for Women and Children and Hit Hospital) during by examining 864 stool samples in a direct examination method, The results revealed the infection rate was 41.7 % and the percentage of infection among males 47.8% is higher than that of females 35.4% with significant differences (p≤0.05). The age groups (1-9) years recorded the highest rates 55.4% and the lowest rate 13.6% in the age group (40-49) years. The highest rate of infection was 62.5% during the month of June, while the month of October was the lowest rate 5% and significant differences. The incidence rate in rural areas was 50.6% higher than in the urban areas 32.5%. The study also included the effect of Teucrium polium L. on the parasite in the culture media HSP-1, the concentrations of 0.5-3 mg / mL significantly affected Giardia, it was noted whenever the greater the concentration, the greater the effect during different treatment periods (1-4) days, as the highest concentration 3 mg/ml killed all Giardia parasites on the fourth day of treatment.

Hepatic Encephalopathy and Liver Failure in Pediatric Patient with hepatitis A

2019 ◽  
Vol 9 (o3) ◽  
Author(s):  
Shaima’a Dakhel AbdulHassa
Keyword(s):  
Hepatic Encephalopathy ◽  
Liver Failure ◽  
Hepatitis A ◽  
Gene Sequence ◽  
Stool Examination ◽  
Age Group ◽  
Significant Difference ◽  
Medical City ◽  
Sequence Method ◽  
Stool Samples

Gairdia lamblia is one of parasites that cause intestinal problems within the human body, particularly private travelers and children. In this study a total of (100) diarrheal patients, 20 patients with Giardiasis were identified by fecal antigen. 9 out of 20(20%) of them were infected by fecal antigen, while 9(9%) of them were infected by using the screening general stool examination (GSE). The stool samples were collected from patient how vested the Medical City/ Baghdad and Tikrit teaching Hospital during the period from 1 st may 2018 to 1 February 2019. The results revealing a significant difference (p andlt; 0.05) between the two methods of detection for G. lamblia (Fecal antigen method and GSE). IT has been shown that out of 20 infected individuals 12(12%) were males and 8(8%) were females, indicating regarding no significant deference in the distribution of Giardiasis among genders. In regard the age, our results showed that highest infection rate 8(3.2%) was recorded in the age group (10-19) years, followed by the age group (20-2) years which was 692.4%). In this study five mutations were recorded at position (926, 1094, 1202and 1304), by using tpiA gene sequence method, and tpiB gene was on point mutation change (G254A), in the position (85) of triose phosphate isomease.

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