Sequential changes in the structural microenvironment of germinal cells during annual spermatogenesis in Asteroids

2020 ◽  
pp. 423-423
Author(s):  
Charles W. Walker
Keyword(s):  
Germinal Cells

scholarly journals Lead Acetate Induced Cytotoxicity in Male Germinal Cells of Swiss Mice.

2003 ◽  
Vol 41 (3) ◽  
pp. 291-294 ◽  
Author(s):  
U.R. ACHARYA ◽  
S. ACHARYA ◽  
M. MISHRA
Keyword(s):  
Lead Acetate ◽  
Swiss Mice ◽  
Germinal Cells

scholarly journals Encephalomyocarditis (EMC) virus-induced testicular lesion in BALB/c mice

1994 ◽  
Vol 28 (4) ◽  
pp. 330-334 ◽  
Author(s):  
M. Shigesato ◽  
K. Hirasawa ◽  
M. Takeda ◽  
K. Doi
Keyword(s):  
Virus Titre ◽  
Male Mice ◽  
Inflammatory Infiltration ◽  
Emc Virus ◽  
Testicular Lesions ◽  
Almost All ◽  
Germinal Cells ◽  
Old Mice

Characteristics of encephalomyocarditis (EMC) virus-induced testicular lesions were investigated in 4- and 8-week-old BALB/c male mice after intraperitoneal (i.p.) and intratesticular (left) (i.t.) inoculation of the D variant of EMC virus (EMC-D). Apart from variation in severity and incidence, the histopathological nature of the resultant testicular lesion was similar in all infected mice, and was characterized by degeneration and necrosis of germinal cells and spermatogonia with inflammatory infiltration. Almost all the inoculated left testes of the i.t. group developed marked lesions. In general, the virus titre in the testis and incidence of testicular lesions were higher in 4-week-old mice than in 8-week-old mice. In addition, testicular lesions developed earlier and with a higher incidence in the PBS-inoculated right testis of the i.t. group than in either testis of the i.p. group of the same age.

Absence of a nuclear androgen receptor in isolated germinal cells of rat testis

1977 ◽  
Vol 9 (2) ◽  
pp. 159-167 ◽  
Author(s):  
J.A. Grootegoed ◽  
M.J. Peters ◽  
E. Mulder ◽  
F.F.G. Rommerts ◽  
H.J. Van Der Molen
Keyword(s):  
Androgen Receptor ◽  
Rat Testis ◽  
Germinal Cells

Echinococcus multilocularis: In vitro secretion of antigen by hybridomas from metacestode germinal cells and murine tumor cells

1989 ◽  
Vol 68 (2) ◽  
pp. 186-191 ◽  
Author(s):  
Angela Dieckmann-Schuppert ◽  
Andreas Ruppel ◽  
Reinhard Burger ◽  
Werner Frank
Keyword(s):  
Tumor Cells ◽  
Echinococcus Multilocularis ◽  
Murine Tumor ◽  
Germinal Cells ◽  
Murine Tumor Cells

Testicular effects of a postnatal GnRH antagonist in domestic cats

2021 ◽  
Keyword(s):  
Gnrh Antagonist ◽  
Domestic Cat ◽  
Domestic Cats ◽  
Low Concentrations ◽  
Cellular Debris ◽  
Reduced Height ◽  
Faecal Samples ◽  
Luminal Area ◽  
Germinal Cells

Abstract The aim of this study was to describe the histological effects of two high postnatal doses of the potent third-generation GnRH antagonist, acyline in the domestic cat testicle. Secondly, the physical, endocrine, and steroidogenic findings of this pharmaceutical protocol are also reported. Twelve postnatal littermate male kittens were administered acyline in a dose of 2.2 mg/100 g SC weekly for 2 weeks (ACY; n = 6), or placebo (PL; n = 6). All the animals were followed up until puberty when they were castrated. Serial faecal samples were collected until the age of 10 weeks for testosterone (T) measurement. The kittens achieved puberty without either age (236.5 ± 19.7 vs. 221.7 ± 23.7 days) or body weight (3.05 ± 0.15 vs. 2.78 ± 0.28 kg, P > 0.05) differences between ACY and PL, respectively. Acyline suppressed faecal T concentrations for 3 weeks (P < 0.01). From the fourth week on, both groups had low concentrations up to the end of the follow-up period (P > 0.05). Histological assessment of the testes showed that ACY cats presented a reduced height of the epithelium (P < 0.01) due to the diminished number of germinal cells accompanied by an enlarged luminal area (P < 0.01) with cellular debris (P < 0.01). The immunostaining of P450c17 also appeared partially diminished in ACY testes.

Changes in mRNA length accompany translational regulation of the somatic and testis-specific cytochrome c genes during spermatogenesis in the mouse

Development ◽  
1990 ◽  
Vol 110 (1) ◽  
pp. 249-257
Author(s):  
L.E. Hake ◽  
A.A. Alcivar ◽  
N.B. Hecht
Keyword(s):  
Cytochrome C ◽  
Translational Regulation ◽  
Northern Blot Analysis ◽  
Size Class ◽  
Mrna Levels ◽  
Germinal Cell ◽  
Somatic Tissues ◽  
Mrna Length ◽  
Pachytene Spermatocytes ◽  
Germinal Cells

The mouse testis contains two isotypes of cytochrome c, which differ in 14 of 104 amino acids: cytochrome cs is present in all somatic tissues and cytochrome cT is testis specific. The regulation of cytochrome cS and cytochrome cT gene expression during spermatogenesis was examined by Northern blot analysis using specific cDNA probes. Total RNA was isolated from adult tissues, enriched germinal cell populations and polysomal gradients of total testis and isolated germinal cells. Three cytochrome cS mRNAs were detected averaging 1.3 kb, 1.1 kb and 0.7 kb in all tissues examined; an additional 1.7 kb mRNA was observed in testis. Isolated germinal cells through prepuberal pachytene spermatocytes contained only the three smaller mRNAs; the 1.7 kb mRNA was enriched in round spermatids. All three smaller cytochrome cS mRNAs were present on polysomes; the 1.7 kb mRNA was non-polysomal. Cytochrome cT mRNA of 0.6-0.9 kb was detected in testis; mRNA levels were low in early spermatogonia and peaked in prepuberal pachytene spermatocytes. In adult pachytene spermatocytes, a subset of the cytochrome cT mRNAs, 0.7-0.9 kb, was present on polysomes; a shortened size class, 0.6-0.75 kb, was non-polysomal. A distinct, primarily non-polysomal, cytochrome cT 0.7 kb mRNA was present in round spermatids. These results indicate that (1) both cytochrome cS and cytochrome cT mRNAs are present in early meiotic cells, (2) a 1.7 kb cytochrome cS mRNA is post-meiotically expressed and non-polysomal and (3) cytochrome cS and cytochrome cT mRNAs are each developmentally and translationally regulated during spermatogenesis.

scholarly journals Toxicity and Reproductive Parameters Impairment of Cypermethrin in Male Guinea Pig (Cavia porcellus)

2018 ◽  
Vol 6 (2) ◽  
pp. 130 ◽  
Author(s):  
Bertin Narcisse Vemo ◽  
Augustave Kenfack ◽  
Ferdinand Ngoula ◽  
Edouard Akono Nantia ◽  
Claude Cedric Njieudeu Ngaleu ◽  
...  
Keyword(s):  
Body Weight ◽  
Guinea Pig ◽  
Sperm Count ◽  
Prostatic Acid Phosphatase ◽  
Domestic Animals ◽  
Seminiferous Tubules ◽  
Reproductive Parameters ◽  
Serum Concentrations ◽  
Total Protein Level ◽  
Germinal Cells

Cypermethrin is a large spectrum action insecticide, globally employed to control pests in agriculture and some human and domestic animals ectoparasites. This study aimed to evaluate its toxicity and reproduction impairment in male guinea pig. Forty adult male guinea pigs were divided into 4 groups and orally submitted to 0, 92, 137.5 and 275 mg/kg body weight/day for 90 days. The weight of the liver increased significantly, while that of kidneys decreased significantly in treated animals compared to controls. Serum concentrations of creatinine, urea, ALAT, ASAT, total cholesterol, prostatic acid phosphatase increased significantly, while the testicular total protein level decreased significantly in groups given the insecticide relatively to the control. The testes weight, libido, serum level of testosterone, mobility, sperm count and the percentage of spermatozoa with entire plasma membrane decreased significantly in animals exposed to cypermethrin with reference to controls. The percentages of abnormal spermatozoa increased significantly in animals submitted to 137.5 or 275 mg/kg body weight (bw) of cypermethrin compared to control ones. On the testis histological sections of pesticide-treated animals, immature germinal cells were observed in the lumen of seminiferous tubules. Cypermethrin was toxic in male guinea pig and damaged reproductive parameters.

L'activité 3β-hydroxystéroïde déshydrogénasique dans les gonades en différenciation de Pleurodeles waltlii (Amphibien, Urodèle); visualisation sur coupes sériées à l'aide d'une nouvelle technique histochimique

Development ◽  
1977 ◽  
Vol 42 (1) ◽  
pp. 29-42
Author(s):  
Par G. Collenot ◽  
A. Collenot
Keyword(s):  
Enzyme Activity ◽  
Posterior Part ◽  
Hydroxysteroid Dehydrogenase ◽  
Serial Sections ◽  
Genital Ridge ◽  
Larval Life ◽  
Ultrastructural Studies ◽  
Pleurodeles Waltlii ◽  
Cytochemical Technique ◽  
Germinal Cells

3β-hydroxysteroid dehydrogenase activity in the larval gonads of Pleurodeles waltlii (amphibian, urodele); a new histochemical technique for serial sections. In order to detect a 3β-hydroxysteroid dehydrogenase (3β-HSDH) activity in the larval gonads of the newt Pleurodeles waltlii an original histochemical method has been derived from the cytochemical technique used by Bara & Anderson (1973) in their ultrastructural studies on the same enzyme activity. The new technique which is described here, allows the incubation in toto of the larval gonads, their paraffin embedding and their sectioning into serial sections of 7 μm thickness. Unisexual, male and female, offsprings were used. The 3β-HSDH activity could be detected, in the larval gonads, at the level of the inner cells which constitute the medulla inside the cavity of the genital ridge, as early as the end of the first third of the larval life, at a stage when the gonads are not yet differentiated. When an ovary differentiates, the enzyme activity remains at the level of the ovarian sac which will constitute the theca of the follicles in the mature ovary. When a testis differentiates, the medullary positive cells which associate with the germinal cells constitute the testicular ampullae or cysts and (later) become negative; but, at the level of the posterior part of the testis, several clusters of interstitial cells showed a 3β-HSDH activity in metamorphosed and juvenile animals. The results are discussed in relation with former reports about the steroid-synthesizing cellular sites in the mature ovaries and testes of urodelean amphibians.

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