VIRUS INACTIVATION OF PLASMA BY METHYLENE BLUE/LIGHT EXPOSURE

The purpose of this article is to provide background information and the current understanding of a less familiar cause of female breast cancer; exposure to ultraviolet light at night. Breast cancer is a common disease that causes significant morbidity and mortality in women. There are several risk factors for breast cancer, most of which are genetic and environmental in nature. An often-overlooked risk factor is exposure to blue light during night shift work, which decreases melatonin production. One of the many cancer-preventing properties of melatonin is to limit estrogen production. Increased lifetime exposure to estrogen is a well-known cause of breast cancer. Awareness of nighttime blue light exposure as a breast cancer risk factor by women doing night shift work and those exposed to nighttime light via smartphones and laptops, is essential information to know so that protective measures can be taken.

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The Alterations in Methylene Blue/Light-Treated Frozen Plasma Proteins Revealed by Proteomics

Transfusion Medicine and Hemotherapy ◽

10.1159/000515119 ◽

2021 ◽

pp. 1-8

Author(s):

Tiange Wu ◽

Xiaoning Wang ◽

Kai Ren ◽

Xiaochen Huang ◽

Jiankai Liu

Keyword(s):

Mass Spectrometry ◽

Methylene Blue ◽

Western Blot ◽

Blue Light ◽

Differentially Expressed ◽

Enzyme Linked Immunosorbent Assay ◽

Differentially Expressed Protein ◽

Significant Difference ◽

Modified Proteins ◽

Frozen Plasma

Introduction: The aim of this study was to investigate the modified proteins in methylene blue/light-treated frozen plasma (MB-FP) compared with fresh frozen plasma (FFP) in order to gain a better application of MB/light-treated plasma in clinic transfusion. Methods: MB-FP and FFP were collected from Changchun central blood station, and a trichloroacetic acid/acetone precipitation method was used to remove albumin for the enrichment of lower abundance proteins. The plasma protein in MB-FP and FFP were separated using two-dimensional gel electrophoresis (2-DE) and the differentially expressed protein spots were analyzed using mass spectrometry. Finally, the differentially expressed proteins were tested using Western blot and enzyme-linked immunosorbent assay (ELISA). Results: Approximately 14 differentially expressed protein spots were detected in the MB-FP, and FFP was chosen as the control. After 2-DE comparison analysis and mass spectrometry, 8 significantly differentially expressed protein spots were identified, corresponding to 6 different proteins, including complement C1r subcomponent (C1R), inter-alpha-trypsin inhibitor heavy chain H4 (ITI-H4), keratin, type II cytoskeletal 1 (KRT1), hemopexin (HPX), fibrinogen gamma chain (FGG), and transthyretin (TTR). Western blot showed no significant difference in the expression level of KRT1 between MB-FP and FFP (p > 0.05). Both Western blot and ELISA indicated that the level of HPX was significantly higher in FFP than in MB-FP (p < 0.05). Conclusion: This comparative proteomics study revealed that some significantly modified proteins occur in MB-FP, such as C1R, ITI-H4, KRT1, HPX, FGG, and TTR. Our findings provide more theoretical data for using MB-FP in transfusion medicine. However, the relevance of the data for the transfusion of methylene blue/light-treated plasma remains unclear. The exact modification of these proteins and the effects of these modified proteins on their functions and their effects in clinical plasma infusion need to be further studied.

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Effects of pre-bedtime blue-light exposure on ratio of deep sleep in healthy young men

Sleep Medicine ◽

10.1016/j.sleep.2021.05.046 ◽

2021 ◽

Author(s):

Masao Ishizawa ◽

Takuya Uchiumi ◽

Miki Takahata ◽

Michiyasu Yamaki ◽

Toshiaki Sato

Keyword(s):

Blue Light ◽

Light Exposure ◽

Young Men ◽

Deep Sleep

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Blue light-induced retinal damage: a brief review and a proposal for examining the hypothetical causal link between person digital device use and retinal injury

Medical Hypothesis, Discovery & Innovation in Optometry ◽

10.51329/mehdioptometry118 ◽

2021 ◽

Vol 1 (3) ◽

pp. 129-134

Author(s):

Michael R. Kozlowski

Keyword(s):

Blue Light ◽

Additional Data ◽

Light Exposure ◽

High Energy ◽

Causal Link ◽

Retinal Damage ◽

Digital Devices ◽

Retinal Injury ◽

Device Use ◽

Harmful Effects

Background: There is growing concern that the increased use of personal digital devices, which emit a high proportion of their light in the blue wavelengths, may have harmful effects on the retina. Extensive historical as well as current research demonstrates that exposure to high energy visible light (blue light) can damage the retina under certain circumstances. There are, however, no studies that directly address whether blue light at the intensities emitted by digital devices can potentially cause such harm. The present review aimed to examine whether blue light exposure from computers, tablets, and cell phones can, when used habitually over a prolonged period of time, be harmful to the retinal. Methods: A search of the literature on blue light-induced retinal damage was performed using a number of scientific search engines, including BioOne Complete™, Google Scholar™, Paperity™, PubMed™, and ScienceOpen™. Studies most significant for addressing the question of possible harmful effects of blue light emitted by personal digital devices were selected from this search and reviewed. Results: The data from the selected studies were summarized and their limitations in addressing the question of whether the blue light from personal digital devices is capable of producing retinal damage were addressed. Based on these limitations, a practical experimental protocol for collecting the additional data needed was proposed. Data from pilot experiments are presented that indicate the practicality of this approach. Conclusions: The currently available data on the effects of blue light on the retina are not sufficient to refute the hypothesis that the use of personal digital devices could, over a lifetime, produce retinal damage. Additional studies, such as those proposed in this article, are needed to resolve this issue.

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Interrelation between Qai’lullah, Blue Light Exposure and Neurocognitive Performances: A Short Review

Journal of Quranic Sciences and Research ◽

10.30880/jqsr.2021.02.02.001 ◽

2021 ◽

Vol 02 (02) ◽

Author(s):

Nur Farhana Fadzil ◽

◽

Siti Amira Othman ◽

Keyword(s):

Job Performance ◽

Blue Light ◽

Memory Consolidation ◽

Light Exposure ◽

Short Review ◽

High Energy ◽

Neurocognitive Performance ◽

Night Sleep ◽

Visual Contrast ◽

The World

Qai’lullah or napping is a phenomenon that is widely practiced in the world. Islam advocates mid-day napping as it is primarily practiced by the Prophet Muhammad (pbuh). Scientists and scholars also acknowledge the benefits beyond this practice after various research and studies done. Hence, this article emphasizes topic of sleep in Islamic insight, their stages of sleeps according to Quran and the practiced of Qai’lullah or mid-day napping. The high-energy blue light exposure from the natural source, Sun and also digital screens reported reduce visual contrast and affect the sharpness and clarity by creating glares lead to mental and physical fatigue. Thus, a short nap in the mid-afternoon helps to boost memory, lift our mood, and improve job performance. The effect associated with qai’lullah are also being reviewed including improved the neurocognitive performance, alertness, recover the loss night sleep and enhanced the quality and increased memory consolidation in people.

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Blue Light Exposure In The Morning and Low-Dose Aripiprazole Administration at Night Combined to Effectively Treat Wake-Up Difficulty due to Prolonged Sleep Time

Biomedical Journal of Scientific & Technical Research ◽

10.26717/bjstr.2019.12.002299 ◽

2019 ◽

Vol 12 (4) ◽

Author(s):

Toshiaki Shiomi

Keyword(s):

Blue Light ◽

Low Dose ◽

Light Exposure ◽

Sleep Time

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Blue light is a universal tactic signal forEscherichia coli

10.1101/211474 ◽

2017 ◽

Author(s):

Tatyana Perlova ◽

Martin Gruebele ◽

Yann R. Chemla

Keyword(s):

Blue Light ◽

Light Exposure ◽

Biological Significance ◽

Proton Motive Force ◽

Motive Force ◽

Swimming Velocity ◽

Bacterial Strains ◽

E Coli ◽

Before And After ◽

Swimming Bacteria

AbstractBlue light has been shown to elicit a tumbling response inE. coli, a non-phototrophic bacterium. The exact mechanism of this phototactic response is still unknown, and its biological significance remains unclear. Here, we quantify phototaxis inE. coliby analyzing single-cell trajectories in populations of free-swimming bacteria before and after light exposure. Bacterial strains expressing only one type of chemoreceptor reveal that all fiveE. colireceptors - Aer, Tar, Tsr, Tap and Trg - are capable of mediating a response to light. In particular, light exposure elicits a running response in Tap-only strain, the opposite of the tumbling response observed for all other strains. Light therefore emerges as a universal stimulus for allE. colichemoreceptors. We also show that blue light exposure causes a reversible decrease in swimming velocity, a proxy for proton motive force. We hypothesize that rather than sensing light directly, chemoreceptors sense light-induced perturbations in proton motive force.ImportanceOur findings provide new insights on the mechanism ofE. coliphototaxis, showing that all five chemoreceptor types respond to light and that their interactions play an important role in cell behavior. Our results also open up new avenues for examining and manipulatingE. colitaxis. Since light is a universal stimulus, it may provide a way to quantify interactions between different types of receptors. Since light is easier to control spatially and temporally than chemicals, it may be used to study swimming behavior in complex environments. Since phototaxis can cause migration ofE. colibacteria in light gradients, light may be used to control bacterial density for studying density-dependent processes in bacteria.

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Acclimating Cucumber Plants to Blue Supplemental Light Promotes Growth in Full Sunlight

Frontiers in Plant Science ◽

10.3389/fpls.2021.782465 ◽

2021 ◽

Vol 12 ◽

Author(s):

Chenqian Kang ◽

Yuqi Zhang ◽

Ruifeng Cheng ◽

Elias Kaiser ◽

Qichang Yang ◽

...

Keyword(s):

Blue Light ◽

Photosynthetic Capacity ◽

Light Exposure ◽

Chlorophyll Concentration ◽

Solar Light ◽

Shoot Biomass ◽

Light Use Efficiency ◽

Production Environment ◽

Full Sunlight ◽

Use Efficiency

Raising young plants is important for modern greenhouse production. Upon transfer from the raising to the production environment, young plants should maximize light use efficiency while minimizing deleterious effects associated with exposure to high light (HL) intensity. The light spectrum may be used to establish desired traits, but how plants acclimated to a given spectrum respond to HL intensity exposure is less well explored. Cucumber (Cucumis sativus) seedlings were grown in a greenhouse in low-intensity sunlight (control; ∼2.7 mol photons m–2 day–1) and were treated with white, red, blue, or green supplemental light (4.3 mol photons m–2 day–1) for 10 days. Photosynthetic capacity was highest in leaves treated with blue light, followed by white, red, and green, and was positively correlated with leaf thickness, nitrogen, and chlorophyll concentration. Acclimation to different spectra did not affect the rate of photosynthetic induction, but leaves grown under blue light showed faster induction and relaxation of non-photochemical quenching (NPQ) under alternating HL and LL intensity. Blue-light-acclimated leaves showed reduced photoinhibition after HL intensity exposure, as indicated by a high maximum quantum yield of photosystem II photochemistry (Fv/Fm). Although plants grown under different supplemental light spectra for 10 days had similar shoot biomass, blue-light-grown plants (B-grown plants) showed a more compact morphology with smaller leaf areas and shorter stems. However, after subsequent, week-long exposure to full sunlight (10.7 mol photons m–2 day–1), B-grown plants showed similar leaf area and 15% higher shoot biomass, compared to plants that had been acclimated to other spectra. The faster growth rate in blue-light-acclimated plants compared to other plants was mainly due to a higher photosynthetic capacity and highly regulated NPQ performance under intermittent high solar light. Acclimation to blue supplemental light can improve light use efficiency and diminish photoinhibition under high solar light exposure, which can benefit plant growth.

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