Changes of Collagen Content in Skin, Femur and Uterus of 17β-Estradiol Benzoate-Treated Rats

Endocrinology ◽  
1966 ◽  
Vol 79 (3) ◽  
pp. 486-492 ◽  
Author(s):  
Q. T. SMITH ◽  
DONNA J. ALLISON
Keyword(s):  
Estradiol Benzoate ◽  
Collagen Content ◽  
17Β Estradiol

scholarly journals Electrochemical sensing of estradiol benzoate using hydroxyapatite with three-dimensional channel frameworks

2017 ◽  
Vol 9 (40) ◽  
pp. 5868-5872
Author(s):  
Yu Wen ◽  
Jing Cui ◽  
Bei Shao ◽  
Zouting Cheng ◽  
Liuzheng Wang ◽  
...  
Keyword(s):  
Daily Life ◽  
Three Dimensional ◽  
Estradiol Benzoate ◽  
Electrochemical Sensing ◽  
Metastatic Tumour ◽  
Tumour Formation ◽  
17Β Estradiol

Ubiquitous estradiol benzoate (17β-estradiol-3-benzoate) in the environment and daily life has become a hot issue in society since it has been proven to be closely associated with metastatic tumour formation.

Modulation of pituitary responsiveness to LHRH by androgens in ovariectomized female rats

1977 ◽  
Vol 55 (2) ◽  
pp. 188-192
Author(s):  
Padmaja N. Kulkarni ◽  
Alan A. Simpson ◽  
William H. Moger
Keyword(s):  
Luteinizing Hormone ◽  
Follicle Stimulating Hormone ◽  
Estradiol Benzoate ◽  
Female Rats ◽  
Ovariectomized Rats ◽  
Luteinizing Hormone Releasing Hormone ◽  
Daily Dose ◽  
17Β Estradiol ◽  
High Doses ◽  
Pronounced Inhibition

The effect of androgens on pituitary response to luteinizing-hormone-releasing hormone (LHRH) and their ability to modify effects of 17β-estradiol (E2) on pituitary responsiveness to LHRH were tested in ovariectomized rats maintained on a daily dose of 0.25 μg estradiol benzoate per rat for 6 d before androgen administration.Testosterone propionate (TP) (4, 40, 400, or 4000 μg per rat), administered 24 h before LHRH (500 ng per rat), had no significant effect on luteinizing hormone (LH) or follicle-stimulating hormone (FSH) response. Similar doses of dihydrotestosterone (DHT) did not significantly alter the LH response but significantly suppressed the FSH response. Even the lowest dose completely blocked the FSH response to LHRH. TP in combination with 4 or 400 μg of E2 suppressed the stimulatory effect of E2 on both LH and FSH response to LHRH in a dose-related manner. DHT and E2 in combination affected LH response inconsistently, whereas their ratio determined FSH response; there was pronounced inhibition of FSH response in rats given high doses of DHT combined with low doses of E2; DHT inhibition of FSH response in animals receiving 4 μg of DHT with 400 μg E2 was partially overcome by the stimulatory effect of E2. Our results indicate that TP and DHT affect LH and FSH response to LHRH differently. The ratio of androgen to estrogen is important in determining the response to LHRH.

17β-Estradiol Benzoate Decreases the AHP Amplitude in CA1 Pyramidal Neurons

2002 ◽  
Vol 88 (2) ◽  
pp. 621-626 ◽  
Author(s):  
Ashok Kumar ◽  
Thomas C. Foster
Keyword(s):  
Pyramidal Neurons ◽  
Brain Aging ◽  
Hippocampal Slices ◽  
Estradiol Benzoate ◽  
Female Rats ◽  
Aged Rats ◽  
Age Related ◽  
Channel Inhibition ◽  
17Β Estradiol ◽  
Dependent Processes

Disruption of Ca2+ homeostasis is hypothesized to mediate several electrophysiological markers of brain aging. Recent evidence indicates that estradiol can rapidly alter Ca2+-dependent processes in neurons through nongenomic mechanisms. In the current study, electrophysiological effects of 17β-estradiol benzoate (EB) on the Ca2+-activated afterhyperpolarization (AHP) were investigated using intracellular sharp electrode recording in hippocampal slices from ovariectomized Fischer 344 female rats. The AHP amplitude was enhanced in aged (22–24 mo) compared with young (5–8 mo) rats and direct application of EB (100 pM) reduced the AHP in aged rats. The age-related difference was due, in part, to the increased AHP amplitude of aged animals, since an EB-mediated decrease in the AHP could be observed in young rats when the extracellular Ca2+ was elevated to increase the AHP amplitude. In aged rats, bath application of EB occluded the ability of the L-channel blocker, nifedipine (10 μM), to attenuate the AHP. The results support a role for EB in modifying hippocampal Ca2+-dependent processes in a manner diametrically opposite that observed during aging, possibly through L-channel inhibition.

scholarly journals Hindbrain Administration of Estradiol Inhibits Feeding and Activates Estrogen Receptor-α-Expressing Cells in the Nucleus Tractus Solitarius of Ovariectomized Rats

Endocrinology ◽  
2007 ◽  
Vol 149 (4) ◽  
pp. 1609-1617 ◽  
Author(s):  
Sumpun Thammacharoen ◽  
Thomas A. Lutz ◽  
Nori Geary ◽  
Lori Asarian
Keyword(s):  
Estrogen Receptor ◽  
Food Intake ◽  
Nucleus Tractus Solitarius ◽  
Estradiol Benzoate ◽  
Estrogen Receptor Α ◽  
Ovariectomized Rats ◽  
Afferent Projections ◽  
Inhibit Food Intake ◽  
17Β Estradiol ◽  
Direct Administration

17β-Estradiol (E2), acting via estrogen receptor (ER)-α, inhibits feeding in animals. One mechanism apparently involves an increase in the satiating potency of cholecystokinin (CCK) released from the small intestine by ingested food. For example, the satiating potency of intraduodenal lipid infusions is increased by E2 in ovariectomized rats; this increased satiation is dependent on CCK, and it is accompanied by increases in the numbers of ERα-positive cells that express c-Fos in a subregion of the caudal nucleus tractus solitarius (cNTS) that receives abdominal vagal afferent projections. To test whether direct administration of E2 to this area of the hindbrain is sufficient to inhibit food intake, we first implanted 0.2 μg estradiol benzoate (EB) in cholesterol or cholesterol alone either sc or onto the surface of the hindbrain over the cNTS. Food intake was significantly reduced after hindbrain EB implants but not after sc EB implants. Next we verified that equimolar hindbrain implants of E2 and EB had similar feeding-inhibitory effects and determined that only small amounts of E2 reached brain areas outside the dorsal caudal hindbrain after hindbrain implants of 3H-labeled E2. Neither plasma estradiol concentration nor plasma inflammatory cytokine concentration was increased by either hindbrain or sc EB implants. Finally, hindbrain EB implants, but not sc implants, increased c-Fos in ERα-positive cells in the cNTS after ip injection of 4 μg/kg CCK-8. We conclude that E2, acting via ERα in cNTS neurons, including neurons stimulated by ip CCK, is sufficient to inhibit feeding.

scholarly journals Optogenetic Activation of Arcuate Kisspeptin Neurons Generates a Luteinizing Hormone Surge-Like Secretion in an Estradiol-Dependent Manner

2021 ◽  
Vol 12 ◽  
Author(s):  
Xian-Hua Lin ◽  
Geffen Lass ◽  
Ling-Si Kong ◽  
Hui Wang ◽  
Xiao-Feng Li ◽  
...  
Keyword(s):  
Luteinizing Hormone ◽  
Brain Area ◽  
Estradiol Benzoate ◽  
Dependent Manner ◽  
Lh Surge ◽  
Expected Time ◽  
17Β Estradiol ◽  
Stimulation Period ◽  
Lh Release ◽  
Lh Secretion

Traditionally, the anteroventral periventricular (AVPV) nucleus has been the brain area associated with luteinizing hormone (LH) surge secretion in rodents. However, the role of the other population of hypothalamic kisspeptin neurons, in the arcuate nucleus (ARC), has been less well characterized with respect to surge generation. Previous experiments have demonstrated ARC kisspeptin knockdown reduced the amplitude of LH surges, indicating that they have a role in surge amplification. The present study used an optogenetic approach to selectively stimulate ARC kisspeptin neurons and examine the effect on LH surges in mice with different hormonal administrations. LH level was monitored from 13:00 to 21:00 h, at 30-minute intervals. Intact Kiss-Cre female mice showed increased LH secretion during the stimulation period in addition to displaying a spontaneous LH surge around the time of lights off. In ovariectomized Kiss-Cre mice, optogenetic stimulation was followed by a surge-like secretion of LH immediately after the stimulation period. Ovariectomized Kiss-Cre mice with a low dose of 17β-estradiol (OVX+E) replacement displayed a surge-like increase in LH release during period of optic stimulation. No LH response to the optic stimulation was observed in OVX+E mice on the day of estradiol benzoate (EB) treatment (day 1). However, after administration of progesterone (day 2), all OVX+E+EB+P mice exhibited an LH surge during optic stimulation. A spontaneous LH surge also occurred in these mice at the expected time. Taken together, these results help to affirm the fact that ARC kisspeptin may have a novel amplificatory role in LH surge production, which is dependent on the gonadal steroid milieu.

scholarly journals PERKEMBANGAN OOSIT INDUK Osteochilus hasselti C.V. YANG DIBERI HORMON ESTRADIOL-17β DAN PAKAN DENGAN KADAR PROTEIN BERBEDA

2014 ◽  
Vol 1 (1) ◽  
pp. 35
Author(s):  
Andri Prajaka Santo ◽  
Untung Susilo ◽  
Gratiana E Wijayanti
Keyword(s):  
Body Weight ◽  
Egg Production ◽  
Estradiol Benzoate ◽  
Randomized Design ◽  
17Β Estradiol ◽  
Completely Randomized Design ◽  
Post Spawning ◽  
Estradiol 17Β ◽  
Different Doses

The availability of fish seed is very important in fish propagation. Good quality of fish seeds were produced by a good brooder which characterized a large number of egg production at spawning. The aims of this research were to evaluate the oocytes development in the hard-lipped Barb (Osteochilus hasselti C.V.) given 17β-estradiol, different percentage of protein in the diet, and their combination. This research was conducted experimentally applying Factorial Completely Randomized Design. The first factor was percentage of protein in the diet consisted of 4 levels namely 25% (P1), 30% (P2), 35% (P3), and 40% (P4), the second factor was dose of 17β-estradiol benzoate (EB) consisted of 3 levels namely 0 µg/kg of body weight (D0), 126 µg/kg of body weight (D1), and 210 µg/kg of body weight (D2), thus there were 12 combinations of treatment with 3 replicates. The results showed that neither of protein proportion or 17β-estradiol affected the proportion of oocytes of any developmental stage (p>0,05) within the first two weeks post spawning. The treatments, however, significantly increased the proportion of oocytes at V3-V5, V6-V7, and post-vitellogenic stages (p<0,05) started at week 4th post spawning. The GSI increased in correlation to the proportions of oocytes at stage ≥ V6 (r=0,701; p<0,01) and the dose of 17β-estradiol (r = 0.357, p <0.05). There was no significant different on GSI amongst the experimental groups. The HSI tend to decrease as the dose of 17β-estradiol increased (r = -0.210, p> 0.05). In conclusion, the percentage of protein in the diet and different doses of 17β-estradiol improved oocyte development of Hard-Lipped Barb (Osteochilus hasselti C.V.).

Modification of Pineal Ultrastructure by Exogenous Hormones

1967 ◽  
Vol 25 ◽  
pp. 170-171
Author(s):  
R. A. Turner ◽  
A. E. Rodin ◽  
D. K. Roberts
Keyword(s):  
Electron Microscopy ◽  
Endoplasmic Reticulum ◽  
Rough Endoplasmic Reticulum ◽  
Estradiol Benzoate ◽  
Female Rats ◽  
List Type ◽  
Type Number ◽  
Pregnant Rats ◽  
Gonadal Atrophy ◽  
Pineal Ultrastructure

There have been many reports which establish a relationship between the pineal and sexual structures, including gonadal hypertrophy after pinealectomy, and gonadal atrophy after injection of pineal homogenates or of melatonin. In order to further delineate this relationship the pineals from 5 groups of female rats were studied by electron microscopy:ControlsPregnant ratsAfter 4 weekly injections of 0.1 mg. estradiol benzoate.After 8 daily injections of 150 mcgm. melatonin (pineal hormone).After 8 daily injections of 3 mg. serotonin (melatonin precursor).No ultrastructural differences were evident between the control, and the pregnancy and melatonin groups. However, the estradiol injected animals exhibited a marked increase in the amount and size of rough endoplasmic reticulum within the pineal cells.

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