Painful Neuroma

Neurosurgery ◽  
1981 ◽  
Vol 9 (5) ◽  
pp. 589-600 ◽  
Author(s):  
Arthur F. Battista ◽  
Humberto M. Cravioto ◽  
Gleb N. Budzilovich
Keyword(s):  
Electron Microscopy ◽  
Connective Tissue ◽  
Significant Role ◽  
Axonal Regeneration ◽  
Light And Electron Microscopy ◽  
Neural Tissue ◽  
Distal Portion ◽  
Neuroma Formation

Abstract Sixteen months after resection of a painful neuroma and fascicle ligation of the proximal nerve segments, the fascicleligated neural tissue was examined by light and electron microscopy. No significant neuroma formation was found in the fascicle-ligated regions. The distal portion consisted mainly of connective tissue fibroblasts with an absence of neural elements. Maintaining the perineurium intact by fascicle ligation seems to alter significantly axonal regeneration in humans, as is indicated by minimal neuroma formation. Similar findings after fascicle ligation in animals have also been reported. This lack of neuroma formation after resection of a painful neuroma and fascicle ligation may play a significant role in relieving pain in patients with painful neuromas.

scholarly journals Immunofluorescence and electron microscopy of the cytoplasmic surface of the human erythrocyte membrane and its interaction with Sendai virus.

1979 ◽  
Vol 83 (2) ◽  
pp. 338-347 ◽  
Author(s):  
M Büechi ◽  
T Bächi
Keyword(s):  
Electron Microscopy ◽  
Plasma Membrane ◽  
Plasma Membranes ◽  
Sendai Virus ◽  
Light And Electron Microscopy ◽  
Distal Portion ◽  
Double Labeling ◽  
Virus Particles ◽  
Viral Antigens ◽  
Cytoplasmic Surface

A method was developed for directly observing the inner surfaces of plasma membranes by light and electron microscopy. Human erythrocytes were attached to cover slips (glass or mica) treated with aminopropylsilane and glutaraldehyde, and then disrupted by direct application of a jet of buffer, which removed the distal portion of the cells, thus exposing the cytoplasmic surface (PS) of the flattened membranes. Antispectrin antibodies and Sendai virus particles were employed as sensitive markers for, respectively, the PS and the external surface (ES) of the membrane; their localization by immunofluorescence or electron microscopy demonstrated that the major asymmetrical features of the plasma membrane were preserved. The fusion of Sendai virus particles with cells was investigated using double-labeling immunofluorescence techniques. Virus adsorbed to the ES of cells at 4 degrees C was not accessible to fluorescein-labeled antibodies applied from the PS side. After incubation at 37 degrees C, viral antigens could be detected at the PS. These antigens, however, remained localized and did not diffuse from the site of attachment, as is usually seen in viral antigens accessible on the ES. They may therefore represent internal viral antigens not incorporated into the plasma membrane as a result of virus-cell fusion.

The ultrastructure of the cardiac Purkinje strand in the dog: a morphometric analysis

1983 ◽  
Vol 217 (1207) ◽  
pp. 191-213 ◽  
Keyword(s):  
Electron Microscopy ◽  
Electrical Properties ◽  
Connective Tissue ◽  
Surface Area ◽  
Series Resistance ◽  
Membrane Surface ◽  
Light And Electron Microscopy ◽  
Total Surface Area ◽  
Membrane Surface Area ◽  
Extracellular Ions

Purkinje strands from both ventricles of adult mongrel dogs were excised, and electrical properties were studied by the voltage-clamp technique. The strands were then examined with light and electron microscopy and structural properties were analysed by morphometric techniques. The canine Purkinje strand contains (by volume) about 28% myocyte and 55% dense outer connective tissue. The remainder of the volume is taken up by the inner shell of loosely packed connective tissue within 10 μm of a myocyte membrane. These volume fractions vary considerably from one strand to another. Clefts less than 10 μm wide occupy 18% of the myocyte volume and clefts less than 1 μm wide occupy 1%. The membrane surface area of the myocytes can be divided into three categories by reference to the size of the adjacent cleft. About 47.8% of the membrane surface area faces clefts wider than 1 μm, another 22.2% faces clefts between 0.1 and 1 μm wide, and the final 30% faces clefts less than 0.1 μm wide. The surface area facing the narrowest clefts (less than 0.1 μm wide) is divided between nexuses 3%, desmosomes 10%, and unspecialized membrane 17% (each figure is expressed as a percentage of the total surface area of myocyte membrane). The canine Purkinje strand has a more favourable anatomy than the sheep Purkinje strand for most physiological experiments. We expect that the complicating effects of series resistance and change in the concentration of extracellular ions will be much smaller than in sheep strands, but still not negligible.

Observations on the nephridial system of the cestode Moniezia expansa (Rud., 1805)

Parasitology ◽  
1969 ◽  
Vol 59 (2) ◽  
pp. 449-459 ◽  
Author(s):  
R. E. Howells
Keyword(s):  
Electron Microscopy ◽  
Fine Structure ◽  
Epithelial Cells ◽  
Connective Tissue ◽  
Intercellular Space ◽  
Technical Assistance ◽  
Light And Electron Microscopy ◽  
Flame Cell ◽  
Research Scholarship ◽  
Research Facilities

The nephridial system of M. expansa has been studied using light and electron microscopy, and a number of histochemical techniques have been used on sections of the worm. The organization of the nephridial system and the fine structure of the flame cells and the nephridial ducts are described. Pores, which connect the nephridial lumen to the intercellular space of the connective tissue, exist at the junction of a flame cell and a nephridial duct. These pores may be considered nephrostomes and the system therefore is not protonephridial as defined by Hyman (1951).The epithelium lining the nephridial ducts has a structure which suggests that it is metabolically active. It is postulated that the beating of the cilia of the flame cells draws fluid into the ducts via the nephrostomes, with absorption and/or secretion of solutes being carried out by the epithelial cells of the duct walls. The function of the nephridial system is discussed.I am grateful to Professor James Brough for the provision of research facilities at the Department of Zoology, University College, Cardiff, andtoDrD. A. Erasmus for much helpful advice during the course of the work. I wish to thank Professors W. Peters and T. Wilson for critically reading the manuscript and Miss M. Williams and Mr T. Davies for expert technical assistance.I also wish to thank the Veterinary Inspector and his staff at the Roath Abattoir, Cardiff, for their kind co-operation and assistance in obtaining material.The work was carried out under the tenure of an S.R.C. research scholarship.

The structure of the antennal heart of Aedes aegypti (Linnaeus)

1997 ◽  
Vol 75 (3) ◽  
pp. 444-458 ◽  
Author(s):  
B. D. Sun ◽  
J. M. Schmidt
Keyword(s):  
Electron Microscopy ◽  
Connective Tissue ◽  
Aedes Aegypti ◽  
Light And Electron Microscopy ◽  
Single Layer ◽  
Epidermal Cells ◽  
Muscle Fibres ◽  
Columnar Cells

The structure of the antennal heart of Aedes aegypti (L.) (Diptera: Culicidae) was observed using light and electron microscopy. The antennal heart consists of several distinct regions including a single layer of columnar cells, the chamber walls, the valve, the z-body, the muscle fibres, and the connective tissue filaments. The columnar cells are structurally similar to secretory and osmoregulatory cells. Features of tendinous epidermal cells typically involved in the attachment of muscles to the cuticle can be observed in various areas of the antennal heart when it is examined as a whole. A model describing the pumping mechanism of the antennal heart in A. aegypti is presented.

scholarly journals Localization of a renal kallikrein immunoreactive-like substance in rat ureter.

1988 ◽  
Vol 36 (12) ◽  
pp. 1463-1469 ◽  
Author(s):  
C Orfila ◽  
J L Bascands ◽  
J M Suc ◽  
J P Girolami
Keyword(s):  
Electron Microscopy ◽  
Endoplasmic Reticulum ◽  
Connective Tissue ◽  
Light And Electron Microscopy ◽  
Normal Serum ◽  
Polyclonal Antiserum ◽  
Urinary Kallikrein ◽  
Renal Kallikrein ◽  
Pap Method ◽  
Immunochemical Techniques

An antibody against rat kallikrein was produced in rabbits and its localization was studied in various organs of the rat to confirm its specificity. The distribution of immunoreactive kallikrein was studied in rat ureter by use of immunochemical techniques. Ureteral tissue was fixed in Zamboni's-glutaraldehyde fixative and immunostained with indirect immunofluorescence and the peroxidase-antiperoxidase (PAP) method for light and electron microscopy. Preabsorption of the primary polyclonal antiserum with purified rat urinary kallikrein and substitution with normal serum were used as controls. By light microscopy, kallikrein was localized in the lamina propria and in the adventitial connective tissue surrounding the entire ureter. Immunoelectron microscopy confirmed this immunolocalization. Immunoreactive kallikrein was concentrated in fibroblasts of connective tissue and was not present in collagen fibers. Immunoreactivity was associated with the Golgi complex, free polyribosomes, and rough endoplasmic reticulum. No immunostaining was observed in other subcellular components of fibroblasts.

Antigen challenge induces a supraglottic but not a subglottic edema in the rat larynx

2005 ◽  
Vol 132 (5) ◽  
pp. 694-700 ◽  
Author(s):  
Anders Niklasson ◽  
Åke Dahlqvist
Keyword(s):  
Electron Microscopy ◽  
Mast Cells ◽  
Connective Tissue ◽  
Light And Electron Microscopy ◽  
Allergen Challenge ◽  
Type I ◽  
Subglottic Region ◽  
Mucosal Mast Cells ◽  
Type I Hypersensitivity ◽  
Head Neck

OBJECTIVE: To examine the reaction of mast cells in the larynx of sensitized rats after allergen challenge and determine whether a type I hypersensitivity reaction can elicit a supraglottic and subglottic edema. STUDY DESIGN: Rats were sensitized with ovalbumin (OA) and challenged 3 weeks later by intravenous injection of either OA or NaCl and perfused with glutaraldehyde. The larynges were removed and processed for light and electron microscopy. Sections from the epiglottis and subglottic region were examined. RESULTS: In challenged animals, the connective tissue mast cells (CTMC) in the epiglottic submucosa, but not the mucosal mast cells (MMC) in epithelium, were significantly reduced compared with the case of controls, and the connective tissue was edematous. In the subglottic region, the number of both MMC and CTMC was reduced (albeit not significantly), and there was no edema in the lamina propria. CONCLUSIONS: Allergen challenge induces a marked edema in the epiglottis but not in the subglottic area. SIGNIFICANCE: The results indicate that the supraglottic and subglottic regions react differently to allergen challenge. (Otolaryngol Head Neck Surg 2005;132:694-700.)

A Possibility of Using Ion-Track Matrices based on Polyesters to Create Artificial Connective Tissue

2021 ◽  
Vol 37 (5) ◽  
pp. 66-71
Author(s):  
D.V. Petrova ◽  
N.A. Koshlan ◽  
I.I. Vinogradov ◽  
Y.V. Bogdanova ◽  
I.V. Koshlan ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Tissue Engineering ◽  
Chemical Composition ◽  
Connective Tissue ◽  
Pore Diameter ◽  
Light And Electron Microscopy ◽  
Chinese Hamster ◽  
Proliferation Rate ◽  
Track Membranes ◽  
Polyethylene Terephtalate

Abstract-The cultivation of Chinese hamster fibroblasts (line V79) on ion-track membranes of various polyesters has been tested. Light and electron microscopy were used to take photographs of polyester membranes with fibroblasts grown on them, which indicated the attachment and proliferation of cells on ion-track adhesive surfaces. The greatest survival of fibroblasts was observed on ion-track membranes with a pore diameter of up to 0.5 μ. It was shown that the chemical composition of the track membrane affects the survival and proliferation rate of Chinese hamster fibroblasts. Polyethylene terephtalate and polyethylene naphtalate membranes provided the highest percentage of grown colonies and the greatest proliferation rate of cells. Track-etched polyester membranes can be used to create adhesive surfaces for the cultivation of fibroblasts and the production of artificial connective tissue. Key words: ion-track membranes and technologies, tissue engineering, fibroblasts, burn therapy

The Morphology of Vacuolated Cells in the Liver Following Administration of Vitamin A.

1973 ◽  
Vol 31 ◽  
pp. 408-409
Author(s):  
Odell T. Minick ◽  
Hidejiro Yokoo ◽  
Fawzia Batti
Keyword(s):  
Electron Microscopy ◽  
Body Weight ◽  
Vitamin A ◽  
Light And Electron Microscopy ◽  
Liver Cells ◽  
Prominent Feature ◽  
Vascular Space ◽  
Vacuolated Cell ◽  
Vacuolated Cells ◽  
Vacuolated Cytoplasm

Vacuolated cells in the liver of young rats were studied by light and electron microscopy following the administration of vitamin A (200 units per gram of body weight). Their characteristics were compared with similar cells found in untreated animals.In rats given vitamin A, cells with vacuolated cytoplasm were a prominent feature. These cells were found mostly in a perisinusoidal location, although some appeared to be in between liver cells (Fig. 1). Electron microscopy confirmed their location in Disse's space adjacent to the sinusoid and in recesses between liver cells. Some appeared to be bordering the lumen of the sinusoid, but careful observation usually revealed a tenuous endothelial process separating the vacuolated cell from the vascular space. In appropriate sections, fenestrations in the thin endothelial processes were noted (Fig. 2, arrow).

Control of Autogenous Vein Grafts Prior to Reimplantation, By Electron Microscopy

1972 ◽  
Vol 30 ◽  
pp. 106-107
Author(s):  
John H. L. Watson ◽  
John L. Swedo ◽  
M. Vrandecic
Keyword(s):  
Electron Microscopy ◽  
Light And Electron Microscopy ◽  
Physiological Saline ◽  
Experimental Conditions ◽  
Vein Grafts ◽  
Arterial Blood ◽  
Saphenous Veins ◽  
Autogenous Vein ◽  
Control Of Parameters ◽  
Post Implantation

The ambient temperature and the nature of the storage fluids may well have significant effects upon the post-implantation behavior of venus autografts. A first step in the investigation of such effects is reported here. Experimental conditions have been set which approximate actual operating room procedures. Saphenous veins from dogs have been used as models in the experiments. After removal from the dogs the veins were kept for two hours under four different experimental conditions, viz at either 4°C or 23°C in either physiological saline or whole canine arterial blood. At the end of the two hours they were prepared for light and electron microscopy. Since no obvious changes or damage could be seen in the veins by light microscopy, even with the advantage of tissue specific stains, it was essential that the control of parameters for successful grafts be set by electron microscopy.

Ultrastructure of two neoplasms in culture compared with original biopsies

1984 ◽  
Vol 42 ◽  
pp. 50-51
Author(s):  
Joseph M. Harb ◽  
James T. Casper ◽  
Vlcki Piaskowski
Keyword(s):  
Electron Microscopy ◽  
Tissue Culture ◽  
Biopsy Specimen ◽  
Cultured Cells ◽  
Light And Electron Microscopy ◽  
Human Tumor ◽  
Soft Agar ◽  
Human Tumor Cells ◽  
Morphological Distinction ◽  
Tumor Types

The application of tissue culture and the newer methodologies of direct cloning and colony formation of human tumor cells in soft agar hold promise as valuable modalities for a variety of diagnostic studies, which include morphological distinction between tumor types by electron microscopy (EM). We present here two cases in which cells in culture expressed distinct morphological features not apparent in the original biopsy specimen. Evaluation of the original biopsies by light and electron microscopy indicated both neoplasms to be undifferentiated sarcomas. Colonies of cells propagated in soft agar displayed features of rhabdomyoblasts in one case, and cultured cells of the second biopsy expressed features of Ewing's sarcoma.

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