Cell—matrix interactions: influence of noncollagenous proteins from dentin on cultured dental cells

Development ◽  
1986 ◽  
Vol 96 (1) ◽  
pp. 195-209
Author(s):  
Herve Lesot ◽  
Anthony J. Smith ◽  
Jean-Marie Meyer ◽  
Aline Staubli ◽  
Jean Victor Ruch
Keyword(s):  
Protein Fractions ◽  
Noncollagenous Proteins ◽  
Embryonic Mouse ◽  
Cytoskeleton Organization ◽  
Preferential Localization ◽  
Basal Pole ◽  
Cell Organization ◽  
Tooth Germs ◽  
Cell Matrix Interactions ◽  
Millipore Filters

Matrix-mediated epitheliomesenchymal interactions control dental cytodifferentiations. Experiments were performed in order to study the effects of noncollagenous proteins extracted from dentin on cultured enamel organs and dental papillae. Seven noncollagenous protein fractions were prepared from rabbit incisor dentin and used as substrates to coat Millipore filters. Embryonic mouse tooth germs were dissociated and the isolated tissues were cultured for 4 days on these different substrates as well as on noncoated Millipore filters. When compared to control cultures, only two protein fractions affected the behaviour of epithelial cells. A slight elongation of the cell body and a preferential localization of the nuclei at the basal pole of the cells in contact with the filter was observed with protein fractions 5 and 6. When dental papillae were cultured on Millipore filters coated either with protein fraction 2 or fraction 6, the mesenchymal cells in contact with the filter elongated, polarized and demonstrated a high metabolic activity. Such modifications in the cell organization, implying changes in the cytoskeleton organization and, or, activity, never occurred spontaneously or in the presence of isolated collagens (I–V), laminin or fibronectin.

Histological and Biochemical Observations of Developing Enameloid of the Sea Bream

1987 ◽  
Vol 1 (2) ◽  
pp. 191-195 ◽  
Author(s):  
K. Kawasaki ◽  
S. Shimoda ◽  
M. Fukae
Keyword(s):  
Proteolytic Activity ◽  
Developmental Stages ◽  
Proteolytic Enzymes ◽  
Sea Bream ◽  
Total Protein Content ◽  
Noncollagenous Proteins ◽  
Saturated Atmosphere ◽  
Tooth Germs ◽  
Water Saturated ◽  
Cryostat Sections

In order to study changes in the enameloid matrix of the Sea Bream during the course of its development, we selected the developmental tooth germs of this fish as representative of three different developmental stages: "chalk-like", "cheese-like", and "soft" enameloid. The protein, calcium, and phosphate contents of each sample were analyzed. The changes of the total protein content in each sample suggest that a major part of the proteins decreased during maturation, although newly formed enameloid of the Sea Bream contains collagen and noncollagenous proteins. The existence of proteolytic activity was examined by placement of undemineralized cryostat sections of unfixed tooth germs on exposed and processed photographic films and then incubation for 30 min in a water-saturated atmosphere at 37°C. Proteolytic activity could be detected in the enameloid matrix, which appeared to be in a "cheese-like" stage. It is suggested that proteolytic enzymes play an important role in the removal of proteins during the maturation of enameloid, although the detailed mechanism of the process is still obscure.

Tissue interactions in embryonic mouse tooth germs

Development ◽  
1970 ◽  
Vol 24 (1) ◽  
pp. 159-171
Author(s):  
Edward J. Kollar ◽  
Grace R. Baird
Keyword(s):  
Enamel Organ ◽  
Oral Epithelium ◽  
Embryonic Mouse ◽  
Tooth Shape ◽  
Structural Specificity ◽  
Dental Papilla ◽  
Tissue Interactions ◽  
Dental Epithelium ◽  
Tooth Germs

The ability of fragments of incisor enamel organ and lip-furrow epithelium from 15- and 16-day old embryonic mice to regulate into harmonious tooth constructions is described. The cervical loop and upper half portions of the incisor enamel organ were confronted with incisor or molar dental papillae. Similar combinations were made from lip-furrow epithelium and incisor or molar papillae. The cultures were grown in the anterior chambers of homologous host eyes. The epithelial fragments from the incisor enamel organ when associated with the dental papillae reconstruct teeth typical in all respects; enamel and dentin matrices are deposited. Lip-furrow epithelium arises from the oral epithelium and is temporally and spatially related to the incisor dental epithelium proper. This ectopic epithelium was confronted by incisor and molar papillae. Harmonious teeth developed in these explants. It is concluded that the ability of the dental papillae to elicit new cytodifferentiative and biochemical syntheses from the lip-furrow epithelium indicates that the dental papillae act inductively during tooth ontogeny. The shape of the teeth reconstructed from enamel organ fragments and lip-furrow epithelium were incisiform or molariform in response to the incisor or molar dental papillae. These data confirm the conclusion that the structural specificity for tooth shape resides in the dental papilla.

Tissue interactions in embryonic mouse tooth germs

Development ◽  
1970 ◽  
Vol 24 (1) ◽  
pp. 173-186
Author(s):  
Edward J. Kollar ◽  
Grace R. Baird
Keyword(s):  
Hair Follicles ◽  
The Other ◽  
Embryonic Mouse ◽  
Plantar Surface ◽  
Tissue Interactions ◽  
Other Hand ◽  
Dental Epithelium ◽  
Tooth Germs

The response of embryonic mouse dental epithelium and mesoderm to tissues of ectopic origin was examined. Isolated molar or incisor mesoderm was confronted with epithelium isolated from the plantar surface of the embryonic mouse foot plate or from the snout. Harmoniously structured teeth were formed from the foot epithelium and incisor or molar mesoderm. These data are interpreted as an unequivocal demonstration of the inductive role of the dental mesenchyme. Teeth were absent in confrontations of dental mesenchyme and snout epithelium. The presence of hair follicles in these explants is described and discussed with reference to other integumental epithelio-mesenchymal interactions. Dental epithelium forms keratinizing surface-like epithelium and invading bands of epithelium in association with foot mesoderm; definitive structures are not formed. On the other hand, when incisor or molar epithelium is associated with snout mesoderm, hair follicles are seen in addition to keratinizing surface-like epithelial configurations. The roles of the epithelial and mesenchymal tissues and the nature of epithelio-mesenchymal interactions in the developing mouse integument are discussed.

The influence of the dental papilla on the development of tooth shape in embryonic mouse tooth germs

Development ◽  
1969 ◽  
Vol 21 (1) ◽  
pp. 131-148
Author(s):  
Edward J. Kollar ◽  
Grace R. Baird
Keyword(s):  
Tissue Differentiation ◽  
Embryonic Mouse ◽  
Tooth Shape ◽  
Structural Specificity ◽  
Dental Papilla ◽  
Epithelial Structure ◽  
Tooth Germs ◽  
Labile State

Studies of epithelio-mesenchymal interactions during embryonic organogenesis have led to a number of conclusions regarding the nature of cellular and tissue differentiation (McLoughlin, 1963; Grobstein, 1967). For example, the importance of both the epithelium and the mesenchyme and the dependence of some systems on a limited number of specific mesenchymal tissues have been pointed out (Hilfer, 1968). Intimately connected with the analysis of the factors that elicit differentiation during such interactions is the question of structural specificity of the differentiated structure. Is the directive for the final form of the structure resident in the epithelium, in the mesoderm, or in both? Can a seemingly stable epithelium undergo transformation to a more labile state and respond to a new interaction with the result that a new epithelial structure is formed (Billingham & Silvers, 1963, 1968)?

scholarly journals Cellular crowding guides and debundles the microtubule cytoskeleton

2019 ◽  
Author(s):  
A. Z. Płochocka ◽  
N. A. Bulgakova ◽  
L. Chumakova
Keyword(s):  
Mathematical Modelling ◽  
Biological Systems ◽  
Follicular Epithelium ◽  
Microtubule Cytoskeleton ◽  
Cytoskeleton Organization ◽  
Cell Organization ◽  
The Mean ◽  
Cellular Components ◽  
The Impact

Cytoplasm is densely packed with macromolecules causing cellular crowding, which alters interactions inside cells and differs between biological systems. Here we investigate the impact of crowding on microtubule cytoskeleton organization. Using mathematical modelling, we find that only anisotropic crowding affects the mean microtubule direction, but any crowding reduces the number of microtubules that form bundles. We validate these predictions in vivo using Drosophila follicular epithelium. Since cellular components are transported along microtubules, our results identify cellular crowding as a novel regulator of this transport and cell organization.

Inositol Hexasulphate, a Casein Kinase Inhibitor, Alters Enamel Formation in Cultured Embryonic Mouse Tooth Germs

2000 ◽  
Vol 79 (10) ◽  
pp. 1794-1801 ◽  
Author(s):  
M.A. Torres-Quintana ◽  
S. Lecolle ◽  
D. Septier ◽  
B. Palmier ◽  
S. Rani ◽  
...  
Keyword(s):  
Kinase Inhibitor ◽  
Casein Kinase ◽  
Enamel Formation ◽  
Embryonic Mouse ◽  
Tooth Germs

scholarly journals Trypanosoma cruzi Infection Induces a Global Host Cell Response in Cardiomyocytes

2011 ◽  
Vol 79 (5) ◽  
pp. 1855-1862 ◽  
Author(s):  
Patricio A. Manque ◽  
Christian Probst ◽  
Mirian C. S. Pereira ◽  
Rita C. P. Rampazzo ◽  
L. Shozo Ozaki ◽  
...  
Keyword(s):  
Trypanosoma Cruzi ◽  
Host Cell ◽  
Cellular Response ◽  
Content Type ◽  
Cytoskeleton Organization ◽  
Host Cell Response ◽  
Cell Matrix ◽  
Matrix Interactions ◽  
Chagasic Cardiomyopathy ◽  
Cell Matrix Interactions

ABSTRACTChagas' disease, caused by the hemoflagellate protozoanTrypanosoma cruzi, affects millions of people in South and Central America. Chronic chagasic cardiomyopathy, the most devastating manifestation of this disease, occurs in approximately one-third of infected individuals. Events associated with the parasite's tropism for and invasion of cardiomyocytes have been the focus of intense investigation in recent years. In the present study, we use murine microarrays to investigate the cellular response caused by invasion of primary murine cardiomyocytes byT. cruzitrypomastigotes. These studies identified 353 murine genes that were differentially expressed during the early stages of invasion and infection of these cells. Genes associated with the immune response, inflammation, cytoskeleton organization, cell-cell and cell-matrix interactions, apoptosis, cell cycle, and oxidative stress are among those affected during the infection. Our data indicate thatT. cruziinduces broad modulations of the host cell machinery in ways that provide insight into how the parasite survives, replicates, and persists in the infected host and ultimately defines the clinical outcome of the infection.

A Resorcinol-Formaldehyde Resin as an Embedding Material for Electron Microscopy of Membranes

1969 ◽  
Vol 27 ◽  
pp. 328-329 ◽  
Author(s):  
J. G. Robertson ◽  
D. F. Parsons
Keyword(s):  
Electron Microscopy ◽  
Osmium Tetroxide ◽  
Neutral Lipids ◽  
Lipid Extraction ◽  
Water Soluble ◽  
Formaldehyde Resin ◽  
Electron Microscope Autoradiography ◽  
Resorcinol Formaldehyde ◽  
Major Disadvantage ◽  
Cell Organization

The extraction of lipids from tissues during fixation and embedding for electron microscopy is widely recognized as a source of possible artifact, especially at the membrane level of cell organization. Lipid extraction is also a major disadvantage in electron microscope autoradiography of radioactive lipids, as in studies of the uptake of radioactive fatty acids by intestinal slices. Retention of lipids by fixation with osmium tetroxide is generally limited to glycolipids, phospholipids and highly unsaturated neutral lipids. Saturated neutral lipids and sterols tend to be easily extracted by organic dehydrating reagents prior to embedding. Retention of the more saturated lipids in embedded tissue might be achieved by developing new cross-linking reagents, by the use of highly water soluble embedding materials or by working at very low temperatures.

Preplate neurons in embryonic mouse cortex: Ultrastructural observations using photoconversion of the fluorescent lipophilic dye dil

1992 ◽  
Vol 50 (1) ◽  
pp. 906-907
Author(s):  
Linda C. Hassinger ◽  
James E. Crandall
Keyword(s):  
Mouse Embryos ◽  
Embryonic Mouse ◽  
Cortical Afferents ◽  
Mouse Cortex ◽  
Carbocyanine Dye ◽  
Increased Sensitivity

We have begun to look directly at small numbers of afferent axons to early generated neurons that form the preplate in the developing mouse cortex. The carbocyanine dye Dil (1’1, dioctadecyl-3,3,3’3’-tetramethyl-indocarbocyanine) has proved especially useful for this goal. DiI labels axons and their terminals with greater sensitivity and without some of the disadvantages of axon filling with HRP. The increased sensitivity provided by labeling embryonic axons with DiI has given us new insights into the development of cortical afferents. For instance, we reported originally that afferents from the thalamus were present below the cortex as early as embryonic day 15 (E15) based on HRP injections into mouse embryos. By using DiI placements into the thalamus in aldehyde-fixed brains, we now know that thalamic fibers reach the cortex 24 hrs earlier.

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