scholarly journals Effect of phenothiazines, disodium ethylenediaminetetraacetic acid and diethyl maleate on in vitro rat colonic transport of cefmetazole and inulin.

1987 ◽  
Vol 10 (2) ◽  
pp. 63-71 ◽  
Author(s):  
TAKUMI SUZUKA ◽  
ATSUSHI FURUYA ◽  
AKIRA KAMADA ◽  
TOSHIAKI NISHIHATA
Keyword(s):  
Ethylenediaminetetraacetic Acid ◽  
Diethyl Maleate ◽  
Disodium Ethylenediaminetetraacetic Acid ◽  
Colonic Transport

scholarly journals CSE (Ceftriaxone+ Sulbactam+ Disodium Edta): A Possible Solution to the Global Antimicrobial Resistance Pandemic

2020 ◽  
Vol 14 (3) ◽  
pp. 2039-2045
Author(s):  
Shalini Upadhyay ◽  
Anita Chakravarti ◽  
Tanisha Bharara ◽  
Sushmita Yadav
Keyword(s):  
Bacterial Infections ◽  
Ethylenediaminetetraacetic Acid ◽  
Beta Lactamase ◽  
Global Public Health ◽  
Bacterial Strains ◽  
First Line ◽  
Significant Treatment ◽  
Mortality And Morbidity ◽  
Disodium Ethylenediaminetetraacetic Acid

The menace of multidrug resistance among bacterial infections is an issue of global public health. Treatment of these superbugs with first line antibiotics is associated with significant treatment failure leading to increased mortality and morbidity. Carbapenems and polymyxins are the saviour antibiotics in case of such infections. But the problem is compounded when these antimicrobials also fail. The addition of beta-lactamase inhibitor like sulbactam and disodium ethylenediaminetetraacetic acid broaden the in vitro antibacterial action of ceftriaxone. This novel combination has been found to be effective in most of the drug resistant bacterial strains.

scholarly journals Ultrafine-Grained Ti-31Mo-Type Composites with HA and Ag, Ta2O5 or CeO2 Addition for Implant Applications

Materials ◽  
2021 ◽  
Vol 14 (3) ◽  
pp. 644
Author(s):  
Patrycja Sochacka ◽  
Mieczyslawa U. Jurczyk ◽  
Kamil Kowalski ◽  
Przemyslaw K. Wirstlein ◽  
Mieczyslaw Jurczyk
Keyword(s):  
Ethylenediaminetetraacetic Acid ◽  
Sample Surface ◽  
Open Circuit ◽  
Periodontal Ligament Fibroblasts ◽  
Second Stage ◽  
Ultrafine Grained ◽  
Normal Human ◽  
Modified Surface ◽  
Synthesized Materials

Ultrafine-grained Ti31Mo alloy and Ti31Mo5HA, Ti31Mo5HA-Ag (or Ta2O5, CeO2) composites with a grain size of approximately 2 μm were produced by the application of mechanical alloying and powder metallurgy. Additionally, the surface of the Ti31Mo alloy was modified. In the first stage, the specimens were immersed in 5M NaOH for 24 h at 60 °C. In the second stage, hydroxyapatite (HA) was deposited on the sample surface. The cathodic deposition at −5 V vs. open circuit potential (OCP) in the electrolyte containing 0.25M CaNa2-EDTA (di-calcium ethylenediaminetetraacetic acid), 0.25M K2HPO4 in 1M NaOH at 120 °C for 2 h was applied. The bulk Ti31Mo alloy is a single β-type phase. In the alkali-modified surface titanium oxide, Ti3O is formed. After hydrothermal treatment, the surface layer mostly consists of the Ca10(PO4)6(OH)2 (81.23%) with about 19% content of CaHPO4·2H2O. Using optical profiler, roughness 2D surface topography parameters were estimated. The in vitro cytocompatibility of synthesized materials was studied. The cell lines of normal human osteoblasts (NHost) and human periodontal ligament fibroblasts (HPdLF) was conducted in the presence of tested biomaterials. Ultrafine-grained Ti-based composites altered with HA and Ag, Ta2O5 or CeO2 have superior biocompatibility than the microcrystalline Ti metal. NHost and HPdLF cells in the contact with the synthesized biomaterial showed stable proliferation activity. Biocompatibility tests carried out indicate that the ultrafine-grained Ti31Mo5HA composites with Ag, Ta2O5, or CeO2 could be a good candidate for implant applications.

Attachment of Oral Cytophaga Species to Hydroxyapatite-Containing Surfaces

1980 ◽  
Vol 29 (2) ◽  
pp. 768-777 ◽  
Author(s):  
Roger A. Celesk ◽  
Jack London
Keyword(s):  
Ethylenediaminetetraacetic Acid ◽  
Root Surface ◽  
Cell Suspensions ◽  
Model Systems ◽  
Proteinase K ◽  
Resting Cells ◽  
Streptococcus Sanguis ◽  
Number Of Binding Sites ◽  
Attachment Process

Model systems simulating the cementum portion of teeth were used to characterize the attachment process by which certain species of oral Cytophaga initiate the colonization of the tooth root surface in vitro. The adsorption of these bacteria to spheroidal hydroxyapatite beads and mechanically powdered root material followed Langmuir isotherm kinetics. From such data, the number of binding sites per 20 mg of substrate and the affinity constants were evaluated for two strains of Cytophaga sp. Resting cells of the two strains tested adhered relatively tenaciously to hydroxyapatite beads in numbers similar to those observed with cells of Streptococcus sanguis . Attachment of bacteria to the substrates was partially inhibited by (i) coating the substrates with human serum or saliva, (ii) pretreating cell suspensions with proteinase K or phospholipase C or D, or (iii) exposing the cells to temperatures greater than 60°C for 15 min. Treating resting cell suspensions with pronase, neuraminidase, phospholipase A2, or 0.1 M ethylenediaminetetraacetic acid had no effect on the attachment process.

scholarly journals Effect of Different Irrigation Solutions on the Diffusion of MTA Cement into the Root Canal Dentin

Materials ◽  
2020 ◽  
Vol 13 (23) ◽  
pp. 5472
Author(s):  
José Pedro Martinho ◽  
Sara França ◽  
Siri Paulo ◽  
Anabela Baptista Paula ◽  
Ana Sofia Coelho ◽  
...  
Keyword(s):  
Root Canal ◽  
Ethylenediaminetetraacetic Acid ◽  
Treatment Success ◽  
Confocal Laser ◽  
Control Group ◽  
Middle Section ◽  
Group 2 ◽  
Root Canal Dentin ◽  
Group 1

(1) Aim: This study aims to analyze the in vitro infiltration of a silicate root canal sealer into dentinal tubules after using different endodontic irrigating solutions. (2) Methods: Twenty-nine teeth with single roots were separated into three groups according to the final irrigation protocol: G1 n = 10) = 17% EDTA (ethylenediaminetetraacetic acid) + 3.0% sodium hypochlorite (NaOCl), G2 (n = 10) = 17% EDTA + 2.0% chlorhexidine and G3 (Control group, n = 9) = 17% EDTA + saline solution. Root canals were filled using cold lateral compaction technique with MTA Fillapex sealer and gutta-percha. The sealer was labeled with rhodamine B. The teeth were segmented at the middle and third apical sections, which were visualized using 10× confocal laser microscopy to determine the sealer penetration percentage. (3) Results: In the apical section, no statistically significant differences were found between the groups regarding sealer penetration. In the middle section, Group 1 obtained the highest percentage, and Group 2 the lowest (p = 0.004). Group 1 also presented statistically significant differences in the Control Group (p = 0.031) and had close sealer penetration values. Meanwhile, the Control Group (p = 0.023) and Group 2 (p = 0.029) revealed a significant decrease of sealer penetration between the apical and middle sections. (4) Conclusion: The obtained results support that final irrigation with NaOCl promoted similar sealer penetration in the apical and middle sections. On the other hand, a significant decrease in the sealer penetration of the middle section was observed for the chlorhexidine and saline groups. Compared to other irrigant solutions, NaOCl promotes more uniform sealer penetration, which can correlate with better sealing and, consequently, higher endodontic treatment success.

Partial purification and characterization of cysteine proteinases in eccrine sweat

1987 ◽  
Vol 252 (6) ◽  
pp. R1119-R1129
Author(s):  
H. Yokozeki ◽  
T. Hibino ◽  
K. Sato
Keyword(s):  
Concanavalin A ◽  
Cathepsin B ◽  
Ethylenediaminetetraacetic Acid ◽  
Sweat Glands ◽  
Partial Purification ◽  
Aminopeptidase Activity ◽  
Specific Substrate ◽  
Cysteine Proteinases ◽  
Eccrine Sweat

Attempts were made to purify and characterize cysteine proteinases in human eccrine sweat and further clarify their origin. Benzoyl-DL-arginine-beta-naphthylamide (BANA) and L-leucine beta-naphthylamide (LeuNA) hydrolases in thermally induced sweat were sequentially purified by Sephacryl S-200 chromatography and chromatofocusing, which yielded two major peaks of BANA hydrolase activity, BANA-I and BANA-II. Both enzymes are cysteine proteinases as evidenced by stimulation of enzymic activity by dithiothreitol and ethylenediaminetetraacetic acid and its inhibition by iodoacetic acid, (PCMB), and trans-epoxysuccinyl-L-leucylamido-(4-guanidino)-butane (E-64). Unlike BANA-II, BANA-I showed an additional aminopeptidase activity, an affinity to concanavalin A-Sepharose but no affinity to organomercurial sepharose and failed to hydrolyze benzyloxycarbonyl-phenylalanyl-arginine 4-methyl 7-coumarylamide (Z-Phe-Arg-NMec), a specific substrate for cathepsin B, which is poorly sensitive to leupeptin [inhibitor constant (Ki) = 1 X 10(-5) M] and relatively heat resistant. These and other characteristics such as its isoelectric points (PI) (= 5.8) and the Km for Arg-NMec (0.1 mM) and BANA (0.71 mM) all support the possibility that BANA-I is closely related to cathepsin H. In contrast, BANA-II is sensitive to Zn2+, leupeptin (Ki = 5.5 X 10(-9) M), is not adsorbed by concanavalin A- (Con-A)Sepharose, but is bound to organomercurial sepharose. It has a specificity to Z-Phe-Arg-NMec but not to Arg-NMec, has the molecular weight of 27, PI of 5.2, the pH optima for BANA (6.0), and the Km for BANA of 3.3 mM and the Km for Z-Phe-Arg-NMec of 0.1 mM. These features resemble those of liver cathepsin B. Leupeptin-sensitive BANA hydrolase was observed in the glandular extract of isolated sweat glands, which was increased after stimulation with methacholine and isoproterenol in vitro. The data are consistent with the notion that cathepsins B- and H-like enzymes are present in eccrine sweat and the former may be derived from the sweat gland.

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