scholarly journals Screening of ZIKA virus infection among dengue-like illness patients with negative RT-PCR for dengue virus in Punjab–Pakistan

2021 ◽  
Vol 37 (3) ◽  
Author(s):  
Somia Iqtadar ◽  
Thuan Huu Vo ◽  
Mehreen Mehmood ◽  
Muhammad Salman ◽  
Mamunur Rahman Malik ◽  
...  
Keyword(s):  
Virus Infection ◽  
Confidence Interval ◽  
Dengue Virus ◽  
Zika Virus ◽  
Chikungunya Virus ◽  
False Negative ◽  
Clinical Samples ◽  
Structural Protein ◽  
Rt Pcr ◽  
Zika Virus Infection

Objective: To detect ZIKV using reverse transcription-polymerase chain reaction (RT-PCR) among clinical samples tested negative for Dengue virus (DENV) by RT-PCR in Punjab, 2016. Methods: A descriptive cross-sectional study was carried out for duration of two months. Total of 506 samples were collected within seven days from onset of illness from all over hospitals of Punjab, Pakistan of which 350 were selected simply randomly to test for presence of ZIKV by using “Trioplex Real-Time RT-PCR Assay (Trioplex)”. Cohen’s kappa coefficient (κ) and 95% confidence interval (CI) were used to assess the degree of concordance between DENV positive results of non-structural protein 1 (NS1) and IgM solid-phase enzyme immunoassay (ELISA). Results: No samples were positive for any ZIKV, DENV or Chikungunya virus (CHIKV) by Trioplex. Among the 350 samples, 26 samples were positive concordant and the degree of concordance between NS1- and IgM-ELISA was 13% and κ coefficient was -0.71 (95% CI -0.79, -0.63). Conclusion: At study time, no samples were positive for ZIKV. Strengthening laboratory capacity to confirm arboviruses for Punjab’s laboratories is warranted. Trioplex RT-PCR has 100% sensitivity so there are nominal chances of false negative results. Establishing syndromic surveillance for Zika and conducting a sero-surveillance survey for Zika in areas with high human and Aedes mosquito density are recommended in Punjab. List of Abbreviations: ZIKV: Zika virus, DENV: Dengue virus, CI: Confidence interval, NS1: Non-structural protein 1, HRP: Horseradish peroxidase, IQR: interquartile range, CHIKV: Chikungunya virus, YF: Yellow fever, JE: Japanese encephalitis, PoE: Points of entry. doi: https://doi.org/10.12669/pjms.37.3.3369 How to cite this:Iqtadar S, Vo TH, Mehmood M, Salman M, Malik MR, Masud F, et al. Screening of ZIKA virus infection among dengue-like illness patients with negative RT-PCR for dengue virus in Punjab–Pakistan. Pak J Med Sci. 2021;37(3):---------. doi: https://doi.org/10.12669/pjms.37.3.3369 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

scholarly journals Zika virus infection in a traveller returning to Europe from Brazil, March 2015

2015 ◽  
Vol 20 (23) ◽  
Author(s):  
L Zammarchi ◽  
D Tappe ◽  
C Fortuna ◽  
M E Remoli ◽  
S Günther ◽  
...  
Keyword(s):  
Virus Infection ◽  
Dengue Virus ◽  
Zika Virus ◽  
Chikungunya Virus ◽  
Healthcare Systems ◽  
Mosquito Vectors ◽  
Zika Virus Infection ◽  
Hands And Feet

We report a case of laboratory-confirmed Zika virus infection imported into Europe from the Americas. The patient developed fever, rash, and oedema of hands and feet after returning to Italy from Brazil in late March 2015. The case highlights that, together with chikungunya virus and dengue virus, three major arboviruses are now co-circulating in Brazil. These arboviruses represent a burden for the healthcare systems in Brazil and other countries where competent mosquito vectors are present.

scholarly journals Zika virus infection in a traveller returning from the Maldives, June 2015

2016 ◽  
Vol 21 (2) ◽  
Author(s):  
Essi Marjana Korhonen ◽  
Eili Huhtamo ◽  
Teemu Smura ◽  
Hannimari Kallio-Kokko ◽  
Markku Raassina ◽  
...  
Keyword(s):  
Virus Infection ◽  
Dengue Virus ◽  
Zika Virus ◽  
Denv Infection ◽  
Distribution Range ◽  
Rt Pcr ◽  
Zikv Infection ◽  
Igm Antibodies ◽  
Zika Virus Infection

We report a Zika virus (ZIKV) infection in a patient with fever and rash after returning to Finland from Maldives, June 2015. The patient had dengue virus (DENV) IgG and IgM antibodies but pan-flavivirus RT-PCR and subsequent sequencing showed presence of ZIKV RNA in urine. Recent association of ZIKV with microcephaly highlights the need for laboratory differentiation of ZIKV from DENV infection and the circulation of ZIKV in areas outside its currently known distribution range.

scholarly journals Distinguishing Secondary Dengue Virus Infection From Zika Virus Infection With Previous Dengue by a Combination of 3 Simple Serological Tests

2017 ◽  
Vol 65 (11) ◽  
pp. 1829-1836 ◽  
Author(s):  
Wen-Yang Tsai ◽  
Han Ha Youn ◽  
Carlos Brites ◽  
Jih-Jin Tsai ◽  
Jasmine Tyson ◽  
...  
Keyword(s):  
Virus Infection ◽  
Dengue Virus ◽  
Zika Virus ◽  
Dengue Virus Infection ◽  
Serological Tests ◽  
Zika Virus Infection

scholarly journals MAIT cells are activated in acute Dengue virus infection and after in vitro Zika virus infection

2018 ◽  
Vol 12 (1) ◽  
pp. e0006154 ◽  
Author(s):  
Dominic Paquin-Proulx ◽  
Vivian I. Avelino-Silva ◽  
Bianca A. N. Santos ◽  
Nathália Silveira Barsotti ◽  
Fabiana Siroma ◽  
...  
Keyword(s):  
Virus Infection ◽  
Dengue Virus ◽  
Zika Virus ◽  
Dengue Virus Infection ◽  
Mait Cells ◽  
Zika Virus Infection

scholarly journals Zika emergence in the French Territories of America and description of first confirmed cases of Zika virus infection on Martinique, November 2015 to February 2016

2016 ◽  
Vol 21 (28) ◽  
Author(s):  
Elise Daudens-Vaysse ◽  
Martine Ledrans ◽  
Noellie Gay ◽  
Vanessa Ardillon ◽  
Sylvie Cassadou ◽  
...  
Keyword(s):  
Virus Infection ◽  
Zika Virus ◽  
Significant Proportion ◽  
Clinical Signs ◽  
Main Tool ◽  
Case Definition ◽  
Epidemiological Surveillance ◽  
Suspected Case ◽  
Rt Pcr ◽  
Zika Virus Infection

Following of the emergence of Zika virus in Brazil in 2015, an epidemiological surveillance system was quickly implemented in the French overseas Territories of America (FTA) according to previous experience with dengue and chikungunya and has detected first cases of Zika. General practitioners and medical microbiologists were invited to report all clinically suspected cases of Zika, laboratory investigations were systematically conducted (RT-PCR). On 18 December, the first autochthonous case of Zika virus infection was confirmed by RT-PCR on French Guiana and Martinique, indicating introduction of Zika virus in FTA. The viral circulation of Zika virus was then also confirmed on Guadeloupe and Saint-Martin. We report here early findings on 203 confirmed cases of Zika virus infection identified by RT-PCR or seroneutralisation on Martinique Island between 24 November 2015 and 20 January 2016. All cases were investigated. Common clinical signs were observed (maculopapular rash, arthralgia, fever, myalgia and conjunctival hyperaemia) among these patients, but the rash, the foundation of our case definition, may be absent in a significant proportion of patients (16%). These results are important for the implementation of a suspected case definition, the main tool for epidemiological surveillance, in territories that may be affected by ZIKV emergence, including Europe.

scholarly journals Evaluation of real-time nucleic acid sequence-based amplification for detection of Chikungunya virus in clinical samples

2009 ◽  
Vol 58 (9) ◽  
pp. 1168-1172 ◽  
Author(s):  
J.-N. Telles ◽  
K. Le Roux ◽  
P. Grivard ◽  
G. Vernet ◽  
A. Michault
Keyword(s):  
Nucleic Acid ◽  
Real Time ◽  
Chikungunya Virus ◽  
False Negative ◽  
Nucleic Acid Sequence ◽  
Clinical Samples ◽  
Acid Extraction ◽  
Plasma Samples ◽  
Rt Pcr ◽  
Nucleic Acid Extraction

The Chikungunya virus (CHIKV) is a member of the genus Alphavirus that is transmitted to humans by Aedes mosquitoes. In 2005 and 2006, the Indian Ocean island of La Réunion was hit with an unprecedented CHIKV fever outbreak that infected 300 000 people. In the present study, we describe the evaluation of real-time nucleic acid sequence-based amplification (RT-NASBA) for the detection of CHIKV in clinical samples. A co-extracted and co-amplified chimerical CHIKV RNA sequence was used as an internal control to eliminate false-negative results. The detection threshold of the assay was determined from quantified CHIKV-positive plasma, and estimated to be 200 copies per NASBA reaction. The specificity of the assay was determined using blast analyses and non-cross-reactivity using an O'nyong-nyong virus culture and 250 CHIKV RT-PCR-negative plasma samples. A 100 % specificity was found and no invalid result was obtained, showing the good quality of the nucleic acid extraction. The assay was then evaluated using 252 CHIKV-positive RT-PCR plasma samples. The samples were all tested positive, including those with low viral load. This evaluation showed that the RT-NASBA is a rapid (5 h from sample nucleic acid extraction to detection), sensitive, specific and reliable method for the routine diagnosis of CHIKV in clinical samples.

scholarly journals Zika Virus Infection in a Cohort of Pregnant Women with Exanthematic Disease in Manaus, Brazilian Amazon

Viruses ◽  
2020 ◽  
Vol 12 (12) ◽  
pp. 1362
Author(s):  
Elijane de Fátima Redivo ◽  
Camila Bôtto Menezes ◽  
Márcia da Costa Castilho ◽  
Marianna Brock ◽  
Evela da Silva Magno ◽  
...  
Keyword(s):  
Virus Infection ◽  
Pregnant Women ◽  
Zika Virus ◽  
Descriptive Analysis ◽  
First Trimester ◽  
Adverse Outcomes ◽  
Rt Pcr ◽  
Zika Virus Infection ◽  
Adverse Pregnancy ◽  
In Pregnancy

The epidemic transmission of Zika virus (ZIKV) in Brazil has been identified as a cause of microcephaly and other neurological malformations in the babies of ZIKV-infected women. The frequency of adverse outcomes of Zika virus infection (ZIKVi) in pregnancy differs depending on the characteristics of exposure to infection, the time of recruitment of research participants, and the outcomes to be observed. This study provides a descriptive analysis—from the onset of symptoms to delivery—of a cohort registered as having maternal ZIKVi in pregnancy, from November 2015 to December 2016. Suspected cases were registered at a referral center for infectious and tropical diseases in Manaus, in the Amazonian region of Brazil. Of 834 women notified, 762 women with confirmed pregnancies were enrolled. Reverse-transcriptase polymerase chain reaction (RT-PCR) confirmed ZIKVi in 42.3% of the cohort. In 35.2% of the cohort, ZIKV was the sole infection identified. Severe adverse pregnancy outcomes (miscarriage, stillbirth, or microcephaly) were observed in both RT-PCR ZIKV-positive (5.0%) and ZIKV-negative (1.8%) cases (RR 3.1; 95% IC 1.4–7.3; p < 0.05), especially during the first trimester of pregnancy (RR 6.2, 95% IC 2.3–16.5; p < 0.001). Although other infectious rash diseases were observed in the pregnant women in the study, having confirmed maternal ZIKVi was the most important risk factor for serious adverse pregnancy events.

scholarly journals Dengue virus serotype 4 and chikungunya virus coinfection in a traveller returning from Luanda, Angola, January 2014

2014 ◽  
Vol 19 (10) ◽  
Author(s):  
R Parreira ◽  
S Centeno-Lima ◽  
A Lopes ◽  
D Portugal-Calisto ◽  
A Constantino ◽  
...  
Keyword(s):  
Virus Infection ◽  
Dengue Virus ◽  
Chikungunya Virus ◽  
Clinical Laboratory ◽  
Phylogenetic Analyses ◽  
Genomic Sequences ◽  
Rt Pcr ◽  
Virus Serotype ◽  
First Time

A concurrent dengue virus serotype 4 and chikungunya virus infection was detected in a woman in her early 50s returning to Portugal from Luanda, Angola, in January 2014. The clinical, laboratory and molecular findings, involving phylogenetic analyses of partial viral genomic sequences amplified by RT-PCR, are described. Although the circulation of both dengue and chikungunya viruses in Angola has been previously reported, to our knowledge this is the first time coinfection with both viruses has been detected there.

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