Dengue virus serotype 4 and chikungunya virus coinfection in a traveller returning from Luanda, Angola, January 2014

A concurrent dengue virus serotype 4 and chikungunya virus infection was detected in a woman in her early 50s returning to Portugal from Luanda, Angola, in January 2014. The clinical, laboratory and molecular findings, involving phylogenetic analyses of partial viral genomic sequences amplified by RT-PCR, are described. Although the circulation of both dengue and chikungunya viruses in Angola has been previously reported, to our knowledge this is the first time coinfection with both viruses has been detected there.

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NS-1 antigen positive Dengue Infection and molecular characterization of Dengue Viruses in a private Medical College Hospitalin Dhaka, Bangladesh

Bangladesh Journal of Medical Science ◽

10.3329/bjms.v17i4.38334 ◽

2018 ◽

Vol 17 (4) ◽

pp. 669-673

Author(s):

Mahmuda Siddiqua ◽

Ahmed Nawsher Alam ◽

AKM Muraduzzaman ◽

Tahmina Shirin

Keyword(s):

Virus Infection ◽

Dengue Virus ◽

Dengue Infection ◽

Medical Science ◽

Cost Effective ◽

Rt Pcr ◽

Dengue Virus Infection ◽

Medical College ◽

Virus Serotype ◽

Ns1 Antigen

Introduction: Detection of dengue virus infection as soon as possible is critical for management of dengue virus infected patients. Immuno-chromatographic (ICT) tests are easy, cost effective method for dengue virus antigen detection.The sensitivity and specificity of ICT should compare with a gold standard test like RT-PCR. Aim of this study was to compare two test methods (ICT and RT-PCR), observe dengue serotype and seasonal impact on dengue infection.Methodology & result: The patients of Ibn Sina Medical College Hospital from October 2015 to October 2017 were tested for dengue NS1 antigen by ICT method. Out of 3201 sample tested 32.39% were found positive and 89 of which were re-tested for RT-PCR for comparison. Eighty eight of 89 NS1 positive cases showed positive by RT-PCR method giving an accuracy of 98.87%. Among the RT-PCR positive cases 45 were further analyzed for serotype. DEN-1, DEN-2 or both DEN- 1 and DEN-2 were found in 21, 23 and 1cases respectively. No cases of DEN-3 or DEN-4 were detected.Conclusion: This study showed that easily available and cost effective dengue NS1 antigen detection method (ICT) is as effective as molecular test (RT-PCR). DEN-1 and DEN-2 serotype were prevalent during last few years in Bangladesh. Continuous monitoring of dengue virus serotype is important for prevention and control of sudden epidemic by other serotype. Alert to be more during post monsoon when the peak of dengue virus infection was observed.Bangladesh Journal of Medical Science Vol.17(4) 2018 p.669-673

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First Complete Genome Sequence of a Chikungunya Virus Strain Isolated from a Patient Diagnosed with Dengue Virus Infection in Malaysia

Genome Announcements ◽

10.1128/genomea.00876-16 ◽

2016 ◽

Vol 4 (4) ◽

Cited By ~ 1

Author(s):

Man Kwan Ooi ◽

Han Ming Gan ◽

Ahmad Rohani ◽

Sharifah Syed Hassan

Keyword(s):

Virus Infection ◽

Dengue Virus ◽

Genome Sequence ◽

Complete Genome Sequence ◽

Complete Genome ◽

Infected Patient ◽

Chikungunya Virus ◽

Dengue Virus Infection ◽

Virus Serotype ◽

Dengue Virus Serotype 2

Here, we report the complete genome sequence of a chikungunya virus coinfection strain isolated from a dengue virus serotype 2-infected patient in Malaysia. This coinfection strain was determined to be of the Asian genotype and contains a novel insertion in the nsP3 gene.

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Nine year trends of dengue virus infection in Mumbai, Western India

Journal of Laboratory Physicians ◽

10.4103/jlp.jlp_169_16 ◽

2017 ◽

Vol 9 (04) ◽

pp. 296-302 ◽

Cited By ~ 5

Author(s):

Jayanthi Shastri ◽

Manita Williamson ◽

Nilima Vaidya ◽

Sachee Agrawal ◽

Om Shrivastav

Keyword(s):

Virus Infection ◽

Dengue Virus ◽

Dengue Fever ◽

Monsoon Season ◽

Western India ◽

Rt Pcr ◽

Dengue Virus Infection ◽

Virus Serotype ◽

Wide Range ◽

Ns1 Antigen

Abstract INTRODUCTION: Dengue virus (DENV) causes a wide range of diseases in humans, from acute febrile illness Dengue fever (DF) to life-threatening Dengue hemorrhagic fever (DHF) or Dengue shock syndrome (DSS). Factors believed to be responsible for spread of Dengue virus infection include explosive population growth, unplanned urban overpopulation with inadequate public health systems, poor standing water and vector control, climate changes and increased international recreational, business, military travel to endemic areas. All of these factors must be addressed to control the spread of Dengue and other mosquito-borne infections. The detection of Dengue virus RNA by reverse transcriptase PCR (RT-PCR) in human serum or plasma samples is highly indicative of acute Dengue fever. Moreover, the method is able to identify the Dengue virus serotype by demonstrating defined sequence homologies in the viral genomic RNA. METHODS AND RESULTS: During the nine year period of this study analysis, 6767 strongly suspected cases were tested by RT-PCR. 1685 (24.9%) were Dengue PCR positive and confirmed as Dengue cases. Observations on the seasonality were based on the nine year's data as the intensity of sampling was at its maximum during monsoon season. Dengue typing was done on 100 positive samples after storage of Dengue RNA at – 80°C. Dengue serotypes were detected in 69 samples of which Dengue 2 was most predominant. 576 samples were processed for NS1 antigen and PCR simultaneously. 19/576 were positive (3.3 %) for NS1 as well as by PCR. 23/576 samples were negative for NS1 antigen, but were positive by RT-PCR. The remaining 534 samples which were negative for NS1 antigen were also negative by Dengue RT-PCR. CONCLUSION: In this study we sought to standardize rapid, sensitive, and specific fluorogenic probe-based RT-PCR assay to screen and serotype a representative range of Dengue viruses that are found in and around Mumbai. Qualitative Dengue virus TaqMan assays could have tremendous utility for the epidemiological investigation of Dengue illness and especially for the study of the viremic response with candidate live-attenuated dengue virus vaccines.

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CORRELATION OF DENGUE VIRUS SEROTYPE AND DVI SEVERITY IN ADULT PATIENTS

INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY ◽

10.24293/ijcpml.v24i2.1322 ◽

2018 ◽

Vol 24 (2) ◽

pp. 185

Author(s):

Suci Andriani ◽

Aryati Aryati ◽

Usman Hadi

Keyword(s):

Virus Infection ◽

Dengue Virus ◽

Real Time ◽

Dengue Hemorrhagic Fever ◽

Hemorrhagic Fever ◽

Adult Patients ◽

Rt Pcr ◽

Dengue Virus Infection ◽

Virus Serotype ◽

Degree Of Severity

The clinical manifestation of dengue virus infection is often not clear, varies widely from mild to severe. Exposure of dengue virus which serotype is different from a previous infection is a risk factor for the severe manifestation of dengue virus infection. Dengue hemorrhagic fever is classified into four degrees of severity based on clinical manifestations and laboratory results. Real-time RT-PCR Dengue can detect dengue virus serotype in early dengue virus infection. The aimed of this study was to prove the correlation between dengue virus serotype and degree of severity in adult patients. This study was a cross-sectional observational design done in February until July 2016. Subjects consisted of 100 dengue virus infection patients. Serum of the patients was examined using Real-time RT-PCR Dengue (Simplexa™ Dengue). It was shown that from 46 patients with DENV-3 serotype was 63%, DENV-2 serotype 17.4%, DENV-1 serotypes 17.4% and mixed infection of DENV-1 and DENV-3 serotype 2.2%. There was not any DENV-4 serotype. Dengue Hemorrhagic Fever (DHF) stage I was 47.8%, DHF stage II was 30.4%, DHF stage III was 10.9% and Dengue Fever was 10.9%. There was not any DHF stage IV. There was not enough evidence that DENV-3 correlated with the degree of severity (p= 0.510). Based on this research, DENV-3 serotype was the dominant serotype prevalent at the Dr. Soetomo Hospital. There was no correlation between viral dengue serotype and severity in dengue adult patients in this study.

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Screening of ZIKA virus infection among dengue-like illness patients with negative RT-PCR for dengue virus in Punjab–Pakistan

Pakistan Journal of Medical Sciences ◽

10.12669/pjms.37.3.3369 ◽

2021 ◽

Vol 37 (3) ◽

Author(s):

Somia Iqtadar ◽

Thuan Huu Vo ◽

Mehreen Mehmood ◽

Muhammad Salman ◽

Mamunur Rahman Malik ◽

...

Keyword(s):

Virus Infection ◽

Confidence Interval ◽

Dengue Virus ◽

Zika Virus ◽

Chikungunya Virus ◽

False Negative ◽

Clinical Samples ◽

Structural Protein ◽

Rt Pcr ◽

Zika Virus Infection

Objective: To detect ZIKV using reverse transcription-polymerase chain reaction (RT-PCR) among clinical samples tested negative for Dengue virus (DENV) by RT-PCR in Punjab, 2016. Methods: A descriptive cross-sectional study was carried out for duration of two months. Total of 506 samples were collected within seven days from onset of illness from all over hospitals of Punjab, Pakistan of which 350 were selected simply randomly to test for presence of ZIKV by using “Trioplex Real-Time RT-PCR Assay (Trioplex)”. Cohen’s kappa coefficient (κ) and 95% confidence interval (CI) were used to assess the degree of concordance between DENV positive results of non-structural protein 1 (NS1) and IgM solid-phase enzyme immunoassay (ELISA). Results: No samples were positive for any ZIKV, DENV or Chikungunya virus (CHIKV) by Trioplex. Among the 350 samples, 26 samples were positive concordant and the degree of concordance between NS1- and IgM-ELISA was 13% and κ coefficient was -0.71 (95% CI -0.79, -0.63). Conclusion: At study time, no samples were positive for ZIKV. Strengthening laboratory capacity to confirm arboviruses for Punjab’s laboratories is warranted. Trioplex RT-PCR has 100% sensitivity so there are nominal chances of false negative results. Establishing syndromic surveillance for Zika and conducting a sero-surveillance survey for Zika in areas with high human and Aedes mosquito density are recommended in Punjab. List of Abbreviations: ZIKV: Zika virus, DENV: Dengue virus, CI: Confidence interval, NS1: Non-structural protein 1, HRP: Horseradish peroxidase, IQR: interquartile range, CHIKV: Chikungunya virus, YF: Yellow fever, JE: Japanese encephalitis, PoE: Points of entry. doi: https://doi.org/10.12669/pjms.37.3.3369 How to cite this:Iqtadar S, Vo TH, Mehmood M, Salman M, Malik MR, Masud F, et al. Screening of ZIKA virus infection among dengue-like illness patients with negative RT-PCR for dengue virus in Punjab–Pakistan. Pak J Med Sci. 2021;37(3):---------. doi: https://doi.org/10.12669/pjms.37.3.3369 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Imunocompetent Mice Model for Dengue Virus Infection

The Scientific World JOURNAL ◽

10.1100/2012/525947 ◽

2012 ◽

Vol 2012 ◽

pp. 1-12 ◽

Cited By ~ 11

Author(s):

Denise Gonçalves ◽

Rafael de Queiroz Prado ◽

Eric Almeida Xavier ◽

Natália Cristina de Oliveira ◽

Paulo Marcos da Matta Guedes ◽

...

Keyword(s):

Animal Model ◽

Virus Infection ◽

Dengue Virus ◽

Viral Disease ◽

Mice Model ◽

Rt Pcr ◽

Dengue Virus Infection ◽

Dengue Disease ◽

The Family ◽

The World

Dengue fever is a noncontagious infectious disease caused by dengue virus (DENV). DENV belongs to the familyFlaviviridae, genusFlavivirus, and is classified into four antigenically distinct serotypes: DENV-1, DENV-2, DENV-3, and DENV-4. The number of nations and people affected has increased steadily and today is considered the most widely spread arbovirus (arthropod-borne viral disease) in the world. The absence of an appropriate animal model for studying the disease has hindered the understanding of dengue pathogenesis. In our study, we have found that immunocompetent C57BL/6 mice infected intraperitoneally with DENV-1 presented some signs of dengue disease such as thrombocytopenia, spleen hemorrhage, liver damage, and increase in production of IFNγand TNFαcytokines. Moreover, the animals became viremic and the virus was detected in several organs by real-time RT-PCR. Thus, this animal model could be used to study mechanism of dengue virus infection, to test antiviral drugs, as well as to evaluate candidate vaccines.

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Chikungunya virus and dengue virus coinfection: a case report from Bangladesh

IMC Journal of Medical Science ◽

10.3329/imcjms.v12i1.35178 ◽

2018 ◽

Vol 12 (1) ◽

pp. 42-43

Author(s):

Muhammad Abdur Rahim ◽

Shahana Zaman ◽

Samira Rahat Afroze ◽

Hasna Fahmima Haque ◽

Farhana Afroz ◽

...

Keyword(s):

Case Report ◽

Virus Infection ◽

Dengue Virus ◽

Chikungunya Virus ◽

Dengue Virus Infection ◽

Emerging Infection ◽

Diagnostic Work ◽

Work Up ◽

Diagnostic Work Up ◽

Body Ache

A case of concurrent chikungunya virus and dengue virus infection is reported here. The patient presented with fever and generalized body ache. Diagnostic work-up revealed chikungunya-dengue co-infection. Dengue is endemic in Bangladesh while chikungunya is a recently emerging infection. As both the viruses are transmitted by a common vector, Aedes spp., such co-infections are likely to increase in coming years.IMC J Med Sci 2018; 12(1): 42-43

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Complete Genome Sequences of Chikungunya Viruses Isolated from Plasma Specimens Collected from Haitians in 2014

Genome Announcements ◽

10.1128/genomea.00148-17 ◽

2017 ◽

Vol 5 (15) ◽

Cited By ~ 4

Author(s):

Sarah K. White ◽

J. Glenn Morris ◽

Maha A. Elbadry ◽

Valery Madsen Beau De Rochars ◽

Bernard A. Okech ◽

...

Keyword(s):

Human Plasma ◽

Complete Genome ◽

Chikungunya Virus ◽

Phylogenetic Analyses ◽

Genomic Sequences ◽

Genome Sequences ◽

Chikungunya Fever ◽

Asian Lineage ◽

Virus Isolates

ABSTRACT Ten chikungunya virus isolates from human plasma collected in Haiti from May to August 2014, in the midst of a chikungunya fever outbreak, were fully sequenced. The resulting genomic sequences are nearly identical, and phylogenetic analyses indicate they belong to the Asian lineage of the virus.

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First Imported Case of Chikungunya Virus Infection in a Travelling Canadian Returning from the Caribbean

Canadian Journal of Infectious Diseases and Medical Microbiology ◽

10.1155/2016/2980297 ◽

2016 ◽

Vol 2016 ◽

pp. 1-5

Author(s):

Christian Therrien ◽

Guillaume Jourdan ◽

Kimberly Holloway ◽

Cécile Tremblay ◽

Michael A. Drebot

Keyword(s):

Virus Infection ◽

Dengue Virus ◽

Neutralizing Antibodies ◽

Chikungunya Virus ◽

Joint Stiffness ◽

Clinical Feature ◽

Chikv Infection ◽

Imported Case ◽

The Caribbean ◽

Hands And Feet

This is the first Canadian case of Chikungunya virus (CHIKV) infection reported in a traveller returning from the Caribbean. Following multiple mosquito bites in Martinique Island in January 2014, the patient presented with high fever, headaches, arthralgia on both hands and feet, and a rash on the trunk upon his return to Canada. Initial serological testing for dengue virus infection was negative. Support therapy with nonsteroidal anti-inflammatory drugs was administered. The symptoms gradually improved 4 weeks after onset with residual arthralgia and morning joint stiffness. This clinical feature prompted the clinician to request CHIKV virus serology which was found to be positive for the presence of IgM and neutralizing antibodies. In 2014, over four hundred confirmed CHIKV infection cases were diagnosed in Canadian travellers returning from the Caribbean and Central America. Clinical suspicion of CHIKV or dengue virus infections should be considered in febrile patients with arthralgia returning from the recently CHIKV endemic countries of the Americas.

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