scholarly journals BeerDeCoded: the open beer metagenome project

F1000Research ◽  
2017 ◽  
Vol 6 ◽  
pp. 1676 ◽  
Author(s):  
Jonathan Sobel ◽  
Luc Henry ◽  
Nicolas Rotman ◽  
Gianpaolo Rando
Keyword(s):  
Fungal Species ◽  
Public Understanding ◽  
Metagenomic Data ◽  
Its Sequencing ◽  
Sample Collection ◽  
For Profit ◽  
Sequencing Technologies ◽  
Wild Yeast ◽  
Detection And Identification ◽  
Understanding Of Science

Next generation sequencing has radically changed research in the life sciences, in both academic and corporate laboratories. The potential impact is tremendous, yet a majority of citizens have little or no understanding of the technological and ethical aspects of this widespread adoption. We designed BeerDeCoded as a pretext to discuss the societal issues related to genomic and metagenomic data with fellow citizens, while advancing scientific knowledge of the most popular beverage of all. In the spirit of citizen science, sample collection and DNA extraction were carried out with the participation of non-scientists in the community laboratory of Hackuarium, a not-for-profit organisation that supports unconventional research and promotes the public understanding of science. The dataset presented herein contains the targeted metagenomic profile of 39 bottled beers from 5 countries, based on internal transcribed spacer (ITS) sequencing of fungal species. A preliminary analysis reveals the presence of a large diversity of wild yeast species in commercial brews. With this project, we demonstrate that coupling simple laboratory procedures that can be carried out in a non-professional environment with state-of-the-art sequencing technologies and targeted metagenomic analyses, can lead to the detection and identification of the microbial content in bottled beer.

scholarly journals BeerDeCoded: the open beer metagenome project

F1000Research ◽  
2017 ◽  
Vol 6 ◽  
pp. 1676 ◽  
Author(s):  
Jonathan Sobel ◽  
Luc Henry ◽  
Nicolas Rotman ◽  
Gianpaolo Rando
Keyword(s):  
Fungal Species ◽  
Public Understanding ◽  
Metagenomic Data ◽  
Its Sequencing ◽  
Sample Collection ◽  
For Profit ◽  
Sequencing Technologies ◽  
Wild Yeast ◽  
Detection And Identification ◽  
Understanding Of Science

Next generation sequencing has radically changed research in the life sciences, in both academic and corporate laboratories. The potential impact is tremendous, yet a majority of citizens have little or no understanding of the technological and ethical aspects of this widespread adoption. We designed BeerDeCoded as a pretext to discuss the societal issues related to genomic and metagenomic data with fellow citizens, while advancing scientific knowledge of the most popular beverage of all. In the spirit of citizen science, sample collection and DNA extraction were carried out with the participation of non-scientists in the community laboratory of Hackuarium, a not-for-profit organisation that supports unconventional research and promotes the public understanding of science. The dataset presented herein contains the targeted metagenomic profile of 39 bottled beers from 5 countries, based on internal transcribed spacer (ITS) sequencing of fungal species. A preliminary analysis reveals the presence of a large diversity of wild yeast species in commercial brews. With this project, we demonstrate that coupling simple laboratory procedures that can be carried out in a non-professional environment, with state-of-the-art sequencing technologies and targeted metagenomic analyses, can lead to the detection and identification of the microbial content in bottled beer.

scholarly journals A Pilot Study on Baseline Fungi and Moisture Indicator Fungi in Danish Homes

2021 ◽  
Vol 7 (2) ◽  
pp. 71
Author(s):  
Birgitte Andersen ◽  
Jens C. Frisvad ◽  
Robert R. Dunn ◽  
Ulf Thrane
Keyword(s):  
Pilot Study ◽  
Indoor Environment ◽  
Fungal Growth ◽  
Fungal Species ◽  
Building Envelope ◽  
Its Sequencing ◽  
Indoor Environments ◽  
Outdoor Air ◽  
Detection And Identification ◽  
Dust Sampling

In many complaint cases regarding bad indoor environments, there is no evidence of visible fungal growth. To determine if the problems are fungi-related, dust sampling is the method of choice among building surveyors. However, there is a need to differentiate between species belonging to a normal, dry indoor environment and species belonging to a damp building envelope. The purposes of this pilot study were to examine which fungal species are present in problem-free Danish homes and to evaluate different detection and identification methods. Analyses showed that the fungal diversity outside was different from the diversity inside and that the composition of fungal species growing indoors was different compared to those found as spores, both indoors and outdoors. Common for most homes were Pseudopithomyceschartarum, Cladosporiumallicinum and Alternaria sect. Infectoriae together with Botrytis spp., Penicilliumdigitatum and Pen. glabrum. The results show that ITS sequencing of dust samples is adequate if supported by thorough building inspections and that food products play as large a role in the composition of the baseline spora as the outdoor air and surrounding vegetation. This pilot study provides a list of baseline fungal species found in Danish homes with a good indoor environment.

Understanding understanding: a model for the public learning of radioactivity

1999 ◽  
Vol 8 (4) ◽  
pp. 267-284 ◽  
Author(s):  
Steve Alsop
Keyword(s):  
Informal Learning ◽  
Background Radiation ◽  
Public Understanding Of Science ◽  
Public Understanding ◽  
Change Model ◽  
Potential Health ◽  
The Public ◽  
Understanding Of Science ◽  
Conceptual Change Model

While much of the work in the public understanding of science has focused on the public's appreciation of science and their familiarity with key scientific concepts, understanding the processes involved in learning science has largely been ignored. This article documents a study of how particular members of the public learn about radiation and radioactivity, and proposes a model to describe their learning—the Informal Conceptual Change Model [ICCM]. ICCM is a multidimensional framework that incorporates three theoretical dimensions—the cognitive, conative, and affective. The paper documents each of these dimensions, and then illustrates the model by drawing upon data collected in a case study. The emphasis of the analysis is on understanding how the members of the public living in an area with high levels of background radiation learn about the science of this potential health threat. The summarizing comments examine the need for a greater awareness of the complexities of informal learning.

The roles of rhetoric in the public understanding of science

1994 ◽  
Vol 3 (1) ◽  
pp. 3-23 ◽  
Author(s):  
Alan G. Gross
Keyword(s):  
Rhetorical Analysis ◽  
Public Understanding Of Science ◽  
Public Understanding ◽  
Minor Role ◽  
Contextual Model ◽  
Deficit Model ◽  
The Public ◽  
Science Rhetoric ◽  
Understanding Of Science ◽  
Social Scientific

In the public understanding of science, rhetoric has two distinct roles: it is both a theory capable of analysing public understanding and an activity capable of creating it. In its analytical role, rhetoric reveals two dominant models of public understanding: the deficit model and the contextual model. In the deficit model, rhetoric acts in the minor role of creating public understanding by accommodating the facts and methods of science to public needs and limitations. In the contextual model, rhetoric and rhetorical analysis play major roles. Rhetorical analysis provides an independent source of evidence to secure social scientific claims; in addition, it supplies the grounds for a rhetoric of reconstruction, one that reconstitutes the fact and facts of science in the public interest.

scholarly journals Training in Astronomy

1998 ◽  
Vol 11 (2) ◽  
pp. 910-911
Author(s):  
M. Gerbaldi
Keyword(s):  
Science Education ◽  
Theoretical Models ◽  
Public Understanding Of Science ◽  
Public Understanding ◽  
Primary Science ◽  
University Colleges ◽  
University Level ◽  
Understanding Of Science ◽  
Physical Laws ◽  
The University

Astronomy offers a unique opportunity for promoting the science teaching in its present crisis. Astronomy can be introduced at various levels and become the medium by which both primary science education and public understanding of science are stimulated.At the University level, astronomy can be introduced in the curricula of university colleges and be a subject for M.Sc. and Ph.D. degrees. Astronomy, can give students the opportunity to work scientifically from observations and known physical laws in order to derive knowledge in another field of science. Astronomy can be taught with less formalism and more experimentation, giving students a feel for the link between a phenomenon and its theoretical representation, and how and why a given observation can be represented by different theoretical models.

“It just goes against the grain.” Public understandings of genetically modified (GM) food in the UK

2002 ◽  
Vol 11 (3) ◽  
pp. 273-291 ◽  
Author(s):  
Alison Shaw
Keyword(s):  
Genetic Modification ◽  
Genetically Modified ◽  
Public Attitudes ◽  
Policy Implications ◽  
Public Understanding ◽  
Empirical Literature ◽  
Gm Food ◽  
Scientific Innovation ◽  
Understanding Of Science ◽  
The Uk

This paper reports on one aspect of qualitative research on public understandings of food risks, focusing on lay understandings of genetically modified (GM) food in a UK context. A range of theoretical, conceptual, and empirical literature on food, risk, and the public understanding of science are reviewed. The fieldwork methods are outlined and empirical data from a range of lay groups are presented. Major themes include: varying “technical” knowledge of science, the relationship between knowledge and acceptance of genetic modification, the uncertainty of scientific knowledge, genetic modification as inappropriate scientific intervention in “nature,” the acceptability of animal and human applications of genetic modification, the appropriate boundaries of scientific innovation, the necessity for GM foods, the uncertainty of risks in GM food, fatalism about avoiding risks, and trust in “experts” to manage potential risks in GM food. Key discussion points relating to a sociological understanding of public attitudes to GM food are raised and some policy implications are highlighted.

scholarly journals Differences between the Leaf Mycobiome of Coffea arabica and Wild Coffee Species and Their Modulation by Caffeine/Chlorogenic Acid Content

2021 ◽  
Vol 9 (11) ◽  
pp. 2296
Author(s):  
Leandro Pio de Sousa ◽  
Oliveiro Guerreiro Filho ◽  
Jorge Maurício Costa Mondego
Keyword(s):  
Chlorogenic Acid ◽  
Acid Content ◽  
Information Seeking ◽  
Coffea Arabica ◽  
Pathogen Resistance ◽  
Quantitative Information ◽  
Fungal Species ◽  
Its Sequencing ◽  
Wild Coffee ◽  
The Relationship

The study of microbes associated with the coffee tree has been gaining strength in recent years. In this work, we compared the leaf mycobiome of the traditional crop Coffea arabica with wild species Coffea racemosa and Coffea stenophylla using ITS sequencing for qualitative information and real-time PCR for quantitative information, seeking to relate the mycobiomes with the content of caffeine and chlorogenic acid in leaves. Dothideomycetes, Wallemiomycetes, and Tremellomycetes are the dominant classes of fungi. The core leaf mycobiome among the three Coffea species is formed by Hannaella, Cladosporium, Cryptococcus, Erythrobasidium, and Alternaria. A network analysis showed that Phoma, an important C. arabica pathogen, is negatively related to six fungal species present in C. racemosa and C. stenophylla and absent in C. arabica. Finally, C. arabica have more than 35 times the concentration of caffeine and 2.5 times the concentration of chlorogenic acid than C. stenophylla and C. racemosa. The relationship between caffeine/chlorogenic acid content, the leaf mycobiome, and genotype pathogen resistance is discussed.

scholarly journals Studying behaviours and attitudes towards science and technology

Impact ◽  
2021 ◽  
Vol 2021 (4) ◽  
pp. 27-29
Author(s):  
Naoko Kato-Nitta
Keyword(s):  
Cultural Capital ◽  
Social Stratification ◽  
Science Communication ◽  
Data Science ◽  
Social Scientist ◽  
Science And Technology ◽  
Public Understanding ◽  
Communication Model ◽  
Statistical Mathematics ◽  
Understanding Of Science

What makes research important is an important philosophical question that is a consideration for many researchers. Further important considerations are the public's perception of science and how an individual's perception of science and technology is shaped. These are some of the complex ideas that social scientist Dr Naoko Kato-Nitta, Department of Statistical Data Science, Institute of Statistical Mathematics, Japan, is exploring. She is working on a series of projects related to public perceptions and attitudes towards different scientific disciplines and fields. She hopes that answering such important questions will facilitate the creation of a science communication model for the public understanding of science. Kato-Nitta's research focuses on human behaviour and psychology and how it relates to issues at the interface of technology and society. A key question that she is seeking to answer from the standpoint of cultural capital is how the extent of the general public's participation in science communication can be determined. In the first research to connect social stratification theory and science communication research, Kato-Nitta divided the concept of Bourdieu's cultural capital into two sub-concepts: scientific and technical cultural capital and literary and artistic cultural capital. She went on to consider how these two types of cultural capital affect the exhibit-viewing behaviours of the general public.

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