scholarly journals Reactive oxygen production induced by near-infrared radiation in three strains of the Chl d-containing cyanobacterium Acaryochloris marina

F1000Research ◽  
2013 ◽  
Vol 2 ◽  
pp. 44 ◽  
Author(s):  
Lars Behrendt ◽  
Marc Staal ◽  
Simona M Cristescu ◽  
Frans JM Harren ◽  
Martin Schliep ◽  
...  
Keyword(s):  
Infrared Radiation ◽  
Near Infrared ◽  
Reactive Oxygen ◽  
Oxygenic Photosynthesis ◽  
Ros Generation ◽  
The Novel ◽  
Light Conditions ◽  
Near Infrared Radiation ◽  
Opposite Behavior ◽  
Acaryochloris Marina

Cyanobacteria in the genus Acaryochloris have largely exchanged Chl a with Chl d, enabling them to harvest near-infrared-radiation (NIR) for oxygenic photosynthesis, a biochemical pathway prone to generate reactive oxygen species (ROS). In this study, ROS production under different light conditions was quantified in three Acaryochloris strains (MBIC11017, HICR111A and the novel strain CRS) using a real-time ethylene detector in conjunction with addition of 2-keto-4-thiomethylbutyric acid, a substrate that is converted to ethylene when reacting with certain types of ROS. In all strains, NIR was found to generate less ROS than visible light (VIS). More ROS was generated if strains MBIC11017 and HICR111A were adapted to NIR and then exposed to VIS, while strain CRS demonstrated the opposite behavior. This is the very first study of ROS generation and suggests that Acaryochloris can avoid a considerable amount of light-induced stress by using NIR instead of VIS for its photosynthesis, adding further evolutionary arguments to their widespread appearance.

Use of near-infrared radiation for oxygenic photosynthesis via photon up-conversion

2012 ◽  
Vol 37 (10) ◽  
pp. 8859-8863 ◽  
Author(s):  
Taras Antal ◽  
Emilia Harju ◽  
Laura Pihlgren ◽  
Mika Lastusaari ◽  
Taina Tyystjärvi ◽  
...  
Keyword(s):  
Infrared Radiation ◽  
Near Infrared ◽  
Oxygenic Photosynthesis ◽  
Near Infrared Radiation

scholarly journals Substantial near-infrared radiation-driven photosynthesis of chlorophyll f-containing cyanobacteria in a natural habitat

eLife ◽  
2020 ◽  
Vol 9 ◽  
Author(s):  
Michael Kühl ◽  
Erik Trampe ◽  
Maria Mosshammer ◽  
Michael Johnson ◽  
Anthony WD Larkum ◽  
...  
Keyword(s):  
Infrared Radiation ◽  
Laser Scanning ◽  
Near Infrared ◽  
Natural Habitat ◽  
Red Light ◽  
Laser Scanning Microscopy ◽  
Oxygenic Photosynthesis ◽  
Confocal Laser ◽  
Near Infrared Radiation ◽  
Molecular Physiology

Far-red absorbing chlorophylls are constitutively present as chlorophyll (Chl) d in the cyanobacterium Acaryochloris marina, or dynamically expressed by synthesis of Chl f, red-shifted phycobiliproteins and minor amounts of Chl d via far-red light photoacclimation in a range of cyanobacteria, which enables them to use near-infrared-radiation (NIR) for oxygenic photosynthesis. While the biochemistry and molecular physiology of Chl f-containing cyanobacteria has been unraveled in culture studies, their ecological significance remains unexplored and no data on their in situ activity exist. With a novel combination of hyperspectral imaging, confocal laser scanning microscopy, and nanoparticle-based O2 imaging, we demonstrate substantial NIR-driven oxygenic photosynthesis by endolithic, Chl f-containing cyanobacteria within natural beachrock biofilms that are widespread on (sub)tropical coastlines. This indicates an important role of NIR-driven oxygenic photosynthesis in primary production of endolithic and other shaded habitats.

scholarly journals Biofilm Growth and Near-Infrared Radiation-Driven Photosynthesis of the Chlorophylld-Containing Cyanobacterium Acaryochloris marina

2012 ◽  
Vol 78 (11) ◽  
pp. 3896-3904 ◽  
Author(s):  
Lars Behrendt ◽  
Verena Schrameyer ◽  
Klaus Qvortrup ◽  
Luisa Lundin ◽  
Søren J. Sørensen ◽  
...  
Keyword(s):  
Blue Light ◽  
Infrared Radiation ◽  
Near Infrared ◽  
Growth Mode ◽  
Population Doubling ◽  
Near Infrared Radiation ◽  
Visible Radiation ◽  
Biofilm Growth ◽  
Content Type ◽  
Acaryochloris Marina

ABSTRACTThe cyanobacteriumAcaryochloris marinais the only known phototroph harboring chlorophyll (Chl)d. It is easy to cultivate it in a planktonic growth mode, andA. marinacultures have been subject to detailed biochemical and biophysical characterization. In natural situations,A. marinais mainly found associated with surfaces, but this growth mode has not been studied yet. Here, we show that theA. marinatype strain MBIC11017 inoculated into alginate beads forms dense biofilm-like cell clusters, as in naturalA. marinabiofilms, characterized by strong O2concentration gradients that change with irradiance. Biofilm growth under both visible radiation (VIS, 400 to 700 nm) and near-infrared radiation (NIR, ∼700 to 730 nm) yielded maximal cell-specific growth rates of 0.38 per day and 0.64 per day, respectively. The population doubling times were 1.09 and 1.82 days for NIR and visible light, respectively. The photosynthesis versus irradiance curves showed saturation at a photon irradiance ofEk(saturating irradiance) >250 μmol photons m−2s−1for blue light but no clear saturation at 365 μmol photons m−2s−1for NIR. The maximal gross photosynthesis rates in the aggregates were ∼1,272 μmol O2mg Chld−1h−1(NIR) and ∼1,128 μmol O2mg Chld−1h−1(VIS). The photosynthetic efficiency (α) values were higher in NIR-irradiated cells [(268 ± 0.29) × 10−6m2mg Chld−1(mean ± standard deviation)] than under blue light [(231 ± 0.22) × 10−6m2mg Chld−1].A. marinais well adapted to a biofilm growth mode under both visible and NIR irradiance and under O2conditions ranging from anoxia to hyperoxia, explaining its presence in natural niches with similar environmental conditions.

scholarly journals Substantial near-infrared radiation-driven photosynthesis of chlorophyll f-containing cyanobacteria in a natural habitat

2019 ◽  
Author(s):  
Michael Kühl ◽  
Erik Trampe ◽  
Maria Mosshammer ◽  
Michael Johnson ◽  
Anthony W. D. Larkum ◽  
...  
Keyword(s):  
Infrared Radiation ◽  
Laser Scanning ◽  
Near Infrared ◽  
Natural Habitat ◽  
Red Light ◽  
Laser Scanning Microscopy ◽  
Oxygenic Photosynthesis ◽  
Confocal Laser ◽  
Near Infrared Radiation ◽  
Molecular Physiology

AbstractFar-red absorbing chlorophylls are constitutively present as Chl d in the cyanobacterium Acaryochloris marina, or dynamically expressed by synthesis of Chl f and red-shifted phycobilins via far-red light photoacclimation in a range of cyanobacteria, which enables them to use near-infrared-radiation (NIR) for oxygenic photosynthesis. While the biochemistry and molecular physiology of Chl f-containing cyanobacteria has been unraveled in culture studies, their ecological significance remains unexplored and no data on their in situ activity exist. With a novel combination of hyperspectral imaging, confocal laser scanning microscopy, and nanoparticle-based O2 imaging, we demonstrate substantial NIR-driven oxygenic photosynthesis by endolithic, Chl f-containing cyanobacteria within natural beachrock biofilms that are widespread on (sub)tropical coastlines. This indicates an important role of NIR-driven oxygenic photosynthesis in primary production of endolithic and other shaded habitats.Impact statementCyanobacteria with chlorophyll f show substantial near-infrared radiation-driven photosynthesis in intertidal habitats.

scholarly journals Hyperthermia by near infrared radiation induced immune cells activation and infiltration in breast tumor

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Wan Fatin Amira Wan Mohd Zawawi ◽  
M. H. Hibma ◽  
M. I. Salim ◽  
K. Jemon
Keyword(s):  
Breast Cancer ◽  
T Cells ◽  
Side Effects ◽  
Immune Cells ◽  
Infrared Radiation ◽  
Near Infrared ◽  
Tumor Regression ◽  
Immunohistochemical Analysis ◽  
Therapeutic Effects ◽  
Near Infrared Radiation

AbstractBreast cancer is the most common cancer that causes death in women. Conventional therapies, including surgery and chemotherapy, have different therapeutic effects and are commonly associated with risks and side effects. Near infrared radiation is a technique with few side effects that is used for local hyperthermia, typically as an adjuvant to other cancer therapies. The understanding of the use of near NIR as a monotherapy, and its effects on the immune cells activation and infiltration, are limited. In this study, we investigate the effects of HT treatment using NIR on tumor regression and on the immune cells and molecules in breast tumors. Results from this study demonstrated that local HT by NIR at 43 °C reduced tumor progression and significantly increased the median survival of tumor-bearing mice. Immunohistochemical analysis revealed a significant reduction in cells proliferation in treated tumor, which was accompanied by an abundance of heat shock protein 70 (Hsp70). Increased numbers of activated dendritic cells were observed in the draining lymph nodes of the mice, along with infiltration of T cells, NK cells and B cells into the tumor. In contrast, tumor-infiltrated regulatory T cells were largely diminished from the tumor. In addition, higher IFN-γ and IL-2 secretion was observed in tumor of treated mice. Overall, results from this present study extends the understanding of using local HT by NIR to stimulate a favourable immune response against breast cancer.

scholarly journals Preparation of Y-Ba-Cu-O thin films by laser evaporation with near-infrared radiation

1989 ◽  
Vol 22 (2) ◽  
pp. 323-326 ◽  
Author(s):  
H Lengfellner ◽  
K F Renk ◽  
P Fickenscher ◽  
W Schindler
Keyword(s):  
Thin Films ◽  
Infrared Radiation ◽  
Near Infrared ◽  
Laser Evaporation ◽  
Near Infrared Radiation
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