scholarly journals Ocean Lagrangian Trajectories (OLTraj): Lagrangian analysis for non-expert users

2021 ◽  
Vol 1 ◽  
pp. 117
Author(s):  
Giorgio Dall'Olmo ◽  
Francesco Nencioli ◽  
Thomas Jackson ◽  
Robert J. W. Brewin ◽  
John A. Gittings ◽  
...  
Keyword(s):  
Technical Skills ◽  
New Product ◽  
Worked Examples ◽  
Biogeochemical Cycles ◽  
Lagrangian Analysis ◽  
Geostrophic Currents ◽  
Set Up

Lagrangian analysis is becoming increasingly important to better understand the ocean's biological and biogeochemical cycles. Yet, biologists and chemists often lack the technical skills required to set up such analyses. Here, we present a new product of pre-computed ocean Lagrangian trajectories (OLTraj) targeting non-expert users, and demonstrate how to use it by means of worked examples. OLTraj is based on satellite-derived geostrophic currents, which allows one to directly compare it with other in-situ or satellite products. We anticipate that OLTraj will foster a new interest in Lagrangian applications in ocean biology and biogeochemistry.

scholarly journals Ocean Lagrangian Trajectories (OLTraj): Lagrangian analysis for non-expert users

2021 ◽  
Vol 1 ◽  
pp. 117
Author(s):  
Giorgio Dall'Olmo ◽  
Francesco Nencioli ◽  
Thomas Jackson ◽  
Robert J. W. Brewin ◽  
John A. Gittings ◽  
...  
Keyword(s):  
Technical Skills ◽  
New Product ◽  
Worked Examples ◽  
Biogeochemical Cycles ◽  
Lagrangian Analysis ◽  
Geostrophic Currents ◽  
Set Up

Lagrangian analysis is becoming increasingly important to better understand the ocean's biological and biogeochemical cycles. Yet, biologists and chemists often lack the technical skills required to set up such analyses. Here, we present a new product of pre-computed ocean Lagrangian trajectories (OLTraj) targeting non-expert users, and demonstrate how to use it by means of worked examples. OLTraj is based on satellite-derived geostrophic currents, which allows one to directly compare it with other in-situ or satellite products. We anticipate that OLTraj will foster a new interest in Lagrangian applications in ocean biology and biogeochemistry.

scholarly journals Comparison of Hydrocarbon-Degrading Consortia from Surface and Deep Waters of the Eastern Mediterranean Sea: Characterization and Degradation Potential

Energies ◽  
2021 ◽  
Vol 14 (8) ◽  
pp. 2246
Author(s):  
Georgia Charalampous ◽  
Efsevia Fragkou ◽  
Konstantinos A. Kormas ◽  
Alexandre B. De Menezes ◽  
Paraskevi N. Polymenakou ◽  
...  
Keyword(s):  
Mediterranean Sea ◽  
Oil Spills ◽  
Eastern Mediterranean ◽  
Sole Source ◽  
Eastern Mediterranean Sea ◽  
Deep Waters ◽  
Degradation Rates ◽  
Polycyclic Aromatic ◽  
Set Up

The diversity and degradation capacity of hydrocarbon-degrading consortia from surface and deep waters of the Eastern Mediterranean Sea were studied in time-series experiments. Microcosms were set up in ONR7a medium at in situ temperatures of 25 °C and 14 °C for the Surface and Deep consortia, respectively, and crude oil as the sole source of carbon. The Deep consortium was additionally investigated at 25 °C to allow the direct comparison of the degradation rates to the Surface consortium. In total, ~50% of the alkanes and ~15% of the polycyclic aromatic hydrocarbons were degraded in all treatments by Day 24. Approximately ~95% of the total biodegradation by the Deep consortium took place within 6 days regardless of temperature, whereas comparable levels of degradation were reached on Day 12 by the Surface consortium. Both consortia were dominated by well-known hydrocarbon-degrading taxa. Temperature played a significant role in shaping the Deep consortia communities with Pseudomonas and Pseudoalteromonas dominating at 25 °C and Alcanivorax at 14 °C. Overall, the Deep consortium showed a higher efficiency for hydrocarbon degradation within the first week following contamination, which is critical in the case of oil spills, and thus merits further investigation for its exploitation in bioremediation technologies tailored to the Eastern Mediterranean Sea.

Use of a crystallization robot to set up sitting-drop vapor-diffusion crystallization andin situcrystallization screens

2000 ◽  
Vol 33 (2) ◽  
pp. 344-349 ◽  
Author(s):  
Christopher F. Snook ◽  
Michael D. Purdy ◽  
Michael C. Wiener
Keyword(s):  
Membrane Proteins ◽  
Protein Crystallization ◽  
Integral Membrane Proteins ◽  
Vapor Diffusion ◽  
Liquid Handling ◽  
General Utility ◽  
Crystallization Experiments ◽  
Automated Screening ◽  
Set Up

A commercial crystallization robot has been modified for use in setting up sitting-drop vapor-diffusion crystallization experiments, and for setting up protein crystallization screensin situ. The primary aim of this effort is the automated screening of crystallization of integral membrane proteins in detergent-containing solutions. However, the results of this work are of general utility to robotic liquid-handling systems. Sources of error that can prevent the accurate dispensing and mixing of solutions have been identified, and include local environmental, machine-specific and solution conditions. Solutions to each of these problems have been developed and implemented.

Exploring Nature's plasticity with a flexible probing tool, and finding new ways for its electronic distribution

2002 ◽  
Vol 30 (2) ◽  
pp. 78-82 ◽  
Author(s):  
M. Beier ◽  
M. Baum ◽  
H. Rebscher ◽  
R. Mauritz ◽  
A. Wixmerten ◽  
...  
Keyword(s):  
Three Dimensional ◽  
In Situ Synthesis ◽  
Light Modulation ◽  
Genomic Assays ◽  
Electronic Distribution ◽  
Spatial Light Modulation ◽  
Microarray Fabrication ◽  
Set Up ◽  
Microarray Format

Concepts and results are described for the use of a single, but extremely flexible, probing tool to address a wide variety of genomic questions. This is achieved by transforming genomic questions into a software file that is used as the design scheme for potentially any genomic assay in a microarray format. Microarray fabrication takes place in three-dimensional microchannel reaction carriers by in situ synthesis based on spatial light modulation. This set-up allows for maximum flexibility in design and realization of genomic assays. Flexibility is achieved at the molecular, genomic and assay levels. We have applied this technology to expression profiling and genotyping experiments.

scholarly journals Monitoring compliance with sulfur content regulations of shipping fuel by in situ measurements of ship emissions

2015 ◽  
Vol 15 (17) ◽  
pp. 10087-10092 ◽  
Author(s):  
L. Kattner ◽  
B. Mathieu-Üffing ◽  
J. P. Burrows ◽  
A. Richter ◽  
S. Schmolke ◽  
...  
Keyword(s):  
Sulfur Content ◽  
Air Pollutant ◽  
Monitoring Site ◽  
Data Set ◽  
The North ◽  
Position Data ◽  
Gas Measurements ◽  
Set Up ◽  
Carbon Dioxide Co2

Abstract. In 1997 the International Maritime Organisation (IMO) adopted MARPOL Annex VI to prevent air pollution by shipping emissions. It regulates, among other issues, the sulfur content in shipping fuels, which is transformed into the air pollutant sulfur dioxide (SO2) during combustion. Within designated Sulfur Emission Control Areas (SECA), the sulfur content was limited to 1 %, and on 1 January 2015, this limit was further reduced to 0.1 %. Here we present the set-up and measurement results of a permanent ship emission monitoring site near Hamburg harbour in the North Sea SECA. Trace gas measurements are conducted with in situ instruments and a data set from September 2014 to January 2015 is presented. By combining measurements of carbon dioxide (CO2) and SO2 with ship position data, it is possible to deduce the sulfur fuel content of individual ships passing the measurement station, thus facilitating the monitoring of compliance of ships with the IMO regulations. While compliance is almost 100 % for the 2014 data, it decreases only very little in 2015 to 95.4 % despite the much stricter limit. We analysed more than 1400 ship plumes in total and for months with favourable conditions, up to 40 % of all ships entering and leaving Hamburg harbour could be checked for their sulfur fuel content.

Genetic gap analysis of wildHordeumtaxa

2012 ◽  
Vol 10 (3) ◽  
pp. 242-253 ◽  
Author(s):  
Holly Vincent ◽  
Roland von Bothmer ◽  
Helmut Knüpffer ◽  
Ahmed Amri ◽  
Jan Konopka ◽  
...  
Keyword(s):  
Gap Analysis ◽  
Plant Genetic Resources ◽  
Ex Situ ◽  
Conservation Strategies ◽  
Gene Pools ◽  
Barley Plant ◽  
Genetic Reserve ◽  
Set Up ◽  
Global Inventory

To facilitate the updating ofin situandex situconservation strategies for wild taxa of the genusHordeumL., a combined ecogeographic survey and gap analysis was undertaken. The analysis was based on the Global Inventory of Barley Plant Genetic Resources held by ICARDA plus additional datasets, resulting in a database containing 17,131 wildHordeumaccessions. The analysis concluded that a genetic reserve should be established in the Mendoza Province of Argentina, as this is the most species-rich area globally forHordeum. A network of reserves should also be set up across the Fertile Crescent in Israel, Palestine, Syria, Jordan, Lebanon and Turkey to provide effective conservation within the centres of diversity for gene pools 1B (Hordeum vulgaresubsp.spontaneum(C. Koch) Thell.) and 2 (Hordeum bulbosumL.). The majority of the species were deemed under-collected, so further collecting missions are required worldwide where possible. Althoughex situandin situconservation strategies have been developed, there needs to be further investigation into the ecological environments thatHordeumspecies occupy to ensure that any adaptive traits expressed are fully conserved. Additionally, studies are required to characterize existing collections and test the viability of rare species accessions held in genebanks to determine whether furtherex situcollections are required alongside the proposedin situconservation.

scholarly journals Coralivoría del gasterópodo Jenneria pustulata (Ovulidae: Pediculariinae) en dos arrecifes coralinos del PNN isla Gorgona

2018 ◽  
Vol 47 (2) ◽  
Author(s):  
Mauro Giovanni Zucconi ◽  
Levy D. Obonaga ◽  
Edgardo Londoño-Cruz
Keyword(s):  
Coral Reefs ◽  
Coral Species ◽  
Size Structure ◽  
Coral Tissue ◽  
Laboratory Conditions ◽  
Set Up ◽  
Biological Dynamics ◽  
Colombian Pacific

Coral reefs are very important and highly biodiverse ecosystems that are exposed to various stressors, including biological ones, such as parasitism and corallivory – the direct consumption of coral tissue by a predator. Knowledge on the effects of corallivory on the coral reefs in the Colombian Pacific is poor. Therefore a study was set up to quantify the abundance of and the corallivory rate by the snail Jenneria pustulata in La Azufrada and Playa Blanca coral reefs (Gorgona Island, Colombia). Snails were manually sampled from the underside of Pocillopora sp. colonies and measured in situ to determine their size structure for each reef. To measure possible damage caused by corallivory, several snails were kept under controlled laboratory conditions for 24 h. Snail sizes and corallivory varied significantly between reefs (P=0.0001; P«0.001). Snails from Playa Blanca were larger than snails from La Azufrada, while corallivory was higher in La Azufrada than in Playa Blanca. Although corallivory rates by J. pustulata are smaller than rates reported for other predators in different coral species, it is recommended to continue this kind of investigations in order to increase the knowledge on biological dynamics of this species and to understand how they affect the reefs at Gorgona Island.

Colonization of depopulated crinoids by symbionts: who comes from the bottom and who from the water column?

2015 ◽  
Vol 95 (8) ◽  
pp. 1607-1612 ◽  
Author(s):  
E.S. Mekhova ◽  
P.Y. Dgebuadze ◽  
V.N. Mikheev ◽  
T.A. Britayev
Keyword(s):  
Water Column ◽  
Field Experiments ◽  
Dispersal Ability ◽  
Long Distance ◽  
Sandy Substrate ◽  
Limited Dispersal ◽  
Set Up ◽  
Almost All ◽  
High Degree

Previous experiments with the comatulid Himerometra robustipinna (Carpenter, 1881) demonstrated intensive host-to-host migration processes for almost all symbiotic species both within host aggregations and among hosts separated by several metres. The aim of this study was to check the ability of symbionts to complete long-distance migrations, by means of two in situ experiments which depopulated the crinoid host. Two different sets of field experiments were set up: exposure of depopulated crinoids (set 1) on stony ‘islands’ isolated from native crinoid assemblages by sandy substrate, and (set 2) in cages suspended in the water column. Hosts from set 1 were exposed for 1, 2, 3 and 4 weeks to assess whether substrate has an influence on the symbionts' long-distance migrations. In set 2 cages were exposed for 10–11 days, aiming to check whether symbionts were able to disperse through the water column with currents. These experiments allow the conclusion that post-settled symbionts can actively migrate among their hosts. Symbionts are able to reach their hosts by employing two different ‘transport corridors’, by drifting or swimming in water column, and by moving on the bottom. Comparison of experimental results allows the division of symbionts into two conventional groups according to the dispersal ability of their post-settled stages: (1) species able to complete long-distance migrations, (2) species unable to migrate or having limited dispersal ability. The finding of the free-living shrimp Periclimenes diversipes Kemp, 1922 in set 2 raises the question about the factors that affect such a high degree of specialization of crinoid assemblages.

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