Oral Administration of a Hop Water Extract Ameliorates the Development of Dermatitis Induced by the Periodical Topical Application of a Mite Antigen in Atopic Dermatitis Model NC/Nga Mice

The extracts of Schisandra chinensis (Turcz.) Baill. (Schisandraceae) have various therapeutic effects, including inflammation and allergy. In this study, gomisin M2 (GM2) was isolated from S. chinensis and its beneficial effects were assessed against atopic dermatitis (AD). We evaluated the therapeutic effects of GM2 on 2,4-dinitrochlorobenzene (DNCB) and Dermatophagoides farinae extract (DFE)-induced AD-like skin lesions with BALB/c mice ears and within the tumor necrosis factor (TNF)-α and interferon (IFN)-γ-stimulated keratinocytes. The oral administration of GM2 resulted in reduced epidermal and dermal thickness, infiltration of tissue eosinophils, mast cells, and helper T cells in AD-like lesions. GM2 suppressed the expression of IL-1β, IL-4, IL-5, IL-6, IL-12a, and TSLP in ear tissue and the expression of IFN-γ, IL-4, and IL-17A in auricular lymph nodes. GM2 also inhibited STAT1 and NF-κB phosphorylation in DNCB/DFE-induced AD-like lesions. The oral administration of GM2 reduced levels of IgE (DFE-specific and total) and IgG2a in the mice sera, as well as protein levels of IL-4, IL-6, and TSLP in ear tissues. In TNF-α/IFN-γ-stimulated keratinocytes, GM2 significantly inhibited IL-1β, IL-6, CXCL8, and CCL22 through the suppression of STAT1 phosphorylation and the nuclear translocation of NF-κB. Taken together, these results indicate that GM2 is a biologically active compound that exhibits inhibitory effects on skin inflammation and suggests that GM2 might serve as a remedy in inflammatory skin diseases, specifically on AD.

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A hot water extract of turmeric (Curcuma longa) suppresses acute ethanol-induced liver injury in mice by inhibiting hepatic oxidative stress and inflammatory cytokine production

Journal of Nutritional Science ◽

10.1017/jns.2016.43 ◽

2017 ◽

Vol 6 ◽

Cited By ~ 16

Author(s):

Ryusei Uchio ◽

Yohei Higashi ◽

Yusuke Kohama ◽

Kengo Kawasaki ◽

Takashi Hirao ◽

...

Keyword(s):

Oxidative Stress ◽

Liver Injury ◽

Oral Administration ◽

Alanine Aminotransferase ◽

Curcuma Longa ◽

Water Extract ◽

Hot Water ◽

Hepatic Tissue ◽

Hot Water Extract ◽

Acute Ethanol

AbstractTurmeric (Curcuma longa) is a widely used spice that has various biological effects, and aqueous extracts of turmeric exhibit potent antioxidant activity and anti-inflammatory activity. Bisacurone, a component of turmeric extract, is known to have similar effects. Oxidative stress and inflammatory cytokines play an important role in ethanol-induced liver injury. This study was performed to evaluate the influence of a hot water extract of C. longa (WEC) or bisacurone on acute ethanol-induced liver injury. C57BL/6 mice were orally administered WEC (20 mg/kg body weight; BW) or bisacurone (60 µg/kg BW) at 30 min before a single dose of ethanol was given by oral administration (3·0 g/kg BW). Plasma levels of aspartate aminotransferase and alanine aminotransferase were markedly increased in ethanol-treated mice, while the increase of these enzymes was significantly suppressed by prior administration of WEC. The increase of alanine aminotransferase was also significantly suppressed by pretreatment with bisacurone. Compared with control mice, animals given WEC had higher hepatic tissue levels of superoxide dismutase and glutathione, as well as lower hepatic tissue levels of thiobarbituric acid-reactive substances, TNF-α protein and IL-6 mRNA. These results suggest that oral administration of WEC may have a protective effect against ethanol-induced liver injury by suppressing hepatic oxidation and inflammation, at least partly through the effects of bisacurone.

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Topical Application of Eupatilin Ameliorates Atopic Dermatitis-Like Skin Lesions in NC/Nga Mice

Annals of Dermatology ◽

10.5021/ad.2017.29.1.61 ◽

2017 ◽

Vol 29 (1) ◽

pp. 61 ◽

Cited By ~ 7

Author(s):

Ji Hyun Lee ◽

Ye Jin Lee ◽

Jun Young Lee ◽

Young Min Park

Keyword(s):

Atopic Dermatitis ◽

Topical Application ◽

Skin Lesions

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The Protective Effect of Oral Application of Corni Fructus on the Disorders of the Cornea, Conjunctiva, Lacrimal Gland and Retina by Topical Particulate Matter 2.5

Nutrients ◽

10.3390/nu13092986 ◽

2021 ◽

Vol 13 (9) ◽

pp. 2986

Author(s):

Hyesook Lee ◽

Min Yeong Kim ◽

Seon Yeong Ji ◽

Da Hye Kim ◽

So Young Kim ◽

...

Keyword(s):

Particulate Matter ◽

Oral Administration ◽

Lacrimal Gland ◽

Water Extract ◽

Cell Loss ◽

Inflammatory Factors ◽

Inner Plexiform Layer ◽

Protective Agent ◽

Corni Fructus ◽

Particulate Matter 2.5

Particulate matter 2.5 (PM2.5) may aggravate dry eye disease (DED). Corni Fructus (CF), which is fruit of Cornus officinalis Sieb. et Zucc., has been reported to have various beneficial pharmacological effects, whereas the effect of CF on the eye is still unknown. Therefore, in this study, we investigated the effect of oral administration of water extract of CF (CFW) on the eye, hematology, and biochemistry in a DED model induced by topical exposure to PM2.5. Furthermore, the efficacy of CFW compared with cyclosporine (CsA), an anti-inflammatory agent, and lutein, the posterior eye-protective agent. Sprague-Dawley rats were topically administered 5 mg/mL PM2.5 in both eyes four times daily for 14 days. During the same period, CFW (200 mg/kg and 400 mg/kg) and lutein (4.1 mg/kg) were orally administered once a day. All eyes of rats in the 0.05% cyclosporine A (CsA)-treated group were topically exposed to 20 μL of CsA, twice daily for 14 days. Oral administration of CFW attenuated the PM2.5-induced reduction of tear secretion and corneal epithelial damage. In addition, CFW protected against goblet cell loss in conjunctiva and overexpression of inflammatory factors in the lacrimal gland following topical exposure to PM2.5. Furthermore, CFW markedly prevented PM2.5-induced ganglion cell loss and recovered the thickness of inner plexiform layer. Meanwhile, CFW treatment decreased the levels of total cholesterol and low-density lipoprotein cholesterol in serum induced by PM2.5. Importantly, the efficacy of CFW was superior or similar to that of CsA and lutein. Taken together, oral administration of CFW may have protective effects against PM2.5-induced DED symptoms via stabilization of the tear film and suppression of inflammation. Furthermore, CFW may in part contribute to improving retinal function and lipid metabolism disorder.

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Interaction of organophosphorus insecticide (Ciovap(R)) with phenothiazine anthelmintic in sheep with or without Haemonchus and Trichostrongylus species infection

10.32469/10355/61075 ◽

1981 ◽

Author(s):

◽

Fouad Kasim Mohammad

Keyword(s):

Oral Administration ◽

Topical Application ◽

Gastrointestinal Parasites ◽

Organophosphorus Insecticide ◽

Cholinesterase Inhibition ◽

Clinical Effects ◽

Clinical Infection ◽

Species Introduction

"This study was undertaken to examine the extent of cholinesterase inhibition and clinical effects of repeated topical application of the organophosphorus insecticide (Ciovap (R)) and the oral administration of phenothiazine anthelmintic either alone or in combination in sheep with or without the added stress of clinical infection with gastrointestinal parasites Haemonchus and Trichostrongylus species."--Introduction.

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