scholarly journals Investigation of genetic diversity of poplar variety samples (populus l.) based on ssr-markers

2017 ◽  
Vol 6 (4) ◽  
pp. 105-111
Author(s):  
Федулова ◽  
Tat'yana Fedulova ◽  
Кондратьева ◽  
Anna Kondrat'eva ◽  
Евлаков ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Genetic Identification ◽  
Populus Alba ◽  
Polymorphic Markers ◽  
Microsatellite Primers ◽  
White Poplar ◽  
Ssr Primers ◽  
Different Origins ◽  
Selection Of ◽  
Variety Testing

The object of the research were 28 plants with valuable breeding genotypes of the initial 40-year-old variety-testing cultures of white poplar (Populus alba L.), gray poplar (P. сanescens Sm.), presented by variety of samples of different origins. The purpose of the research was the selection of the most effective microsatellite primers for genetic identification of valuable breeding genotypes of poplar. The selection of high-polymorphic markers was conducted by testing a group of 12 SSR primers.

scholarly journals Comparative analysis of genetic diversity among the maize inbred lines (Zea mays L.) obtained by RAPD and SSR markers

2008 ◽  
Vol 51 (1) ◽  
pp. 183-192 ◽  
Author(s):  
Silvia Graciele Hülse de Souza ◽  
Valéria Carpentieri-Pípolo ◽  
Claudete de Fátima Ruas ◽  
Valdemar de Paula Carvalho ◽  
Paulo Maurício Ruas ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Ssr Markers ◽  
Zea Mays L ◽  
Inbred Lines ◽  
Pedigree Data ◽  
Dice Coefficient ◽  
Quantitative Characters ◽  
Maize Inbred Lines ◽  
Ssr Primers ◽  
Selection Of

The RAPD and SSR markers were used to compare the genetic diversity among the 16 maize inbred lines. Twenty-two primers were used in the RAPD reactions, resulting in the amplification of 265 fragments, while 16 pairs of SSR primers resulted in 75 fragments. The similarity based on Dice coefficient for the RAPD ranged from 53 to 84% and for the SSR from 11 to 82%. The dendrogram obtained by the RAPD showed five groups, while dendrogram obtained by the SSR showed three groups and one isolated line. The association constructed from the markers and the principal coordinate’s analysis separated lines into two groups according to endosperm color, either orange or yellow. The RAPD were effective to validate pedigree data, while the SSR were effective to recognize the differences between the quantitative characters. Because they assess the distinct regions of the genome, the selection of one or other marker would depend on the characteristics of the material used and the objectives of the project.

scholarly journals Evaluation of physiological indicators of plants of the genus Populus in arid conditions of Zhezkazgan

2020 ◽  
Vol 100 (4) ◽  
pp. 66-71
Author(s):  
Gulzhanat Maxutbekova ◽  
Keyword(s):  
Climatic Conditions ◽  
Green Spaces ◽  
Populus Alba ◽  
Populus Balsamifera ◽  
White Poplar ◽  
Physiological Indicators ◽  
Arid Conditions ◽  
Industrial Regions ◽  
Resistance Assessment ◽  
Selection Of

The creation of modern green spaces should be based on the selection of a sustainable assortment. Resistance is evaluated on the basis of a number of physiological indicators. Preliminary testing is of particular value for industrial regions with difficult climatic conditions. The purpose of this study was to study the resistance of 4 species of poplars to drought, winter conditions, the effects of diseases and pests, and atmospheric pollution. Resistance assessment was carried out for the species Populus alba, Populus balsamifera, Populus canadensis, Populus nigra, covering the period from 2015 to 2019. The results of visual observations showed that all species of poplars have high winter resistance — 4–5 points. Maximum winter resistance is noted for white poplar, minimum — for canadian poplar. The gas stability of poplars is estimated at 3–5 points. The greatest stability is noted in balsamic poplar, the minimum — in canadian poplar. Drought resistance among poplar species is estimated at 3–4 points. White and canadian poplar showed the least resistance to drought, while canadian poplar showed the maximum. Estimated scores of resistance to diseases and pests were 4–5 points, the highest indicators were noted for balsamic poplar, the minimum — for black poplar. On the basis of the identified indicators, the directions of poplar use in green construction are proposed.

Assessment of molecular divergence using microsatellite markers linked to drought-yield QTLs of rice (Oryza sativa L.)

2020 ◽  
Vol 57 (2) ◽  
pp. 94-99
Author(s):  
ND Rathan ◽  
SK Singh ◽  
RK Singh ◽  
PR Vennela
Keyword(s):  
Genetic Diversity ◽  
Oryza Sativa ◽  
Oryza Sativa L ◽  
Polymorphic Marker ◽  
Polymorphic Markers ◽  
Rainfed Conditions ◽  
Transgressive Segregants ◽  
Rice Genotypes ◽  
Contemporary Study ◽  
Selection Of

The drought stress is the major limitation for the production and yield stability in the rice (Oryza sativa L.) growing areas particularly under rainfed conditions. The assessment of genetic diversity among the rice genotypes using molecular markers linked to drought-yield QTLs will help in the selection of divergent parents to produce superior recombinants and transgressive segregants to develop high yielding cultivars for drought conditions. So the current study was conducted to estimate the genetic diversity among the set of twenty four advanced drought tolerent rice genotypes and commercial checks using SSR markers linked to major drought-yield QTLs. A total of 31 alleles were detected by 13 polymorphic markers across 24 rice genotypes with a mean of 1.94 alleles per polymorphic marker. Among the polymorphic markers, 11 markers formed 2 alleles each, 2 markers have produced 3 alleles each. The PIC value observed in the contemporary study ranged from 0.239 to 0.499 with an average PIC value of 0.346. The dendrogram displayed a total five clusters on the basis of dissimilarity coefficient values, the cluster II had maximum ten genotypes followed by cluster IB-a with five genotypes and clusters IA-a, IA-b, IB-b with three genotypes each. IR 82475-110-2-2-1-2 and IR92937-178-2-2 (R-155) were found to be the most divergent genotypes.

Genetic diversity in European and Argentinian cultivated potatoes (Solanum tuberosum subsp. tuberosum) detected by inter-simple sequence repeats (ISSRs)

Genome ◽  
2002 ◽  
Vol 45 (3) ◽  
pp. 481-484 ◽  
Author(s):  
B Bornet ◽  
F Goraguer ◽  
G Joly ◽  
M Branchard
Keyword(s):  
Genetic Diversity ◽  
Solanum Tuberosum ◽  
Simple Sequence Repeats ◽  
Genetic Identification ◽  
Germplasm Management ◽  
Polymorphic Markers ◽  
Good Tool ◽  
Inter Simple Sequence Repeats ◽  
Cultivated Potatoes ◽  
Simple Sequence

In this study, the use of inter-simple sequence repeats (ISSR) to assess genetic diversity between cultivated potatoes (Solanum tuberosum subsp. tuberosum) is reported. ISSR technology rapidly reveals high polymorphic fingerprints and thus determines the genetic diversity among potato cultivars. Nine primers were selected according to the number of amplified markers and the level of polymorphism detected. Three primers (GAG(CAA)5, CTG(AG)8, and (AG)8) were used to cluster the 28 potato accessions and 77 polymorphic markers were sufficient to identify all of the accessions. Among the 15 simple sequence repeat (SSR) motifs tested, the most abundant were CAA and AG. Argentinian- and European-grown potatoes were easily distinguished, with a higher level of genetic diversity among potatoes from Argentina. An ISSR study using a limited number of cultivars and very few primers clearly differentiated between all cultivars, thus ISSR was revealed to be a good tool for the genetic identification of potato and for future germplasm-management programs.Key words: Solanum tuberosum subsp. tuberosum, potato, ISSR, genetic diversity.

scholarly journals Genetic diversity analysis of soybean (Glycine max (L.) Merr.) genotypes making use of SSR markers

2019 ◽  
pp. 1113-1119
Author(s):  
Keitumetse Kujane ◽  
Moosa M Sedibe ◽  
Alina Mofokeng
Keyword(s):  
Genetic Diversity ◽  
Ssr Markers ◽  
Polymorphic Information Content ◽  
Molecular Data ◽  
Dna Ladder ◽  
Ssr Primers ◽  
Soybean Genotypes ◽  
Glycine Max L ◽  
Simple Sequence ◽  
Selection Of

In this study, we aimed to investigate the genetic diversity and polymorphism among 30 soybean genotypes maintained by the ARC using simple sequence repeat (SSR) markers. Soybean genotypes were characterized using 20 SSR primers. DNA was extracted using the standard cetyl trimethylammonium bromide method and amplified using PCR. Allele size was determined via comparison with a 100 base pair (bp) DNA ladder. Molecular data were analyzed, and a dendrogram and matrix were generated using GGT 2.0 software. A total of 216 alleles with an average of 10.8 alleles per locus were detected. The allele sizes ranged between 2 and 33 bp with an average of 18.7 bp. The polymorphic information content among genotypes varied from 0.85 (Satt001) to 0.75 (Satt43) with an average of 0.716, and heterozygosity ranged from 0.87 to 0.78 with an average of 0.7485. The most diverse genotypes were B 66 S 31, 69S 7, and R5-4-2 M, which indicated the efficiency of the SSR markers for the detection of genetic diversity. The results of the current study revealed the diversity among the soybean genotypes tested, which might aid breeders in the future in the selection of parents for breeding.

scholarly journals Morphological and SSR Marker Based Diversity Analysis of Lentil (Lens esculenta) Genotypes using Yield and Yield Contributing Characters

2020 ◽  
Author(s):  
M. A. Malek ◽  
M. M. Chowdhury ◽  
M. A. Haque ◽  
M. Rasel ◽  
M. R. Molla ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Market Price ◽  
Group Method ◽  
Improvement Program ◽  
Breeding Programs ◽  
Pair Group ◽  
Ssr Primers ◽  
Selection For ◽  
Set Up ◽  
Selection Of

Lentil is an important pulse crop with high nutritional value and high market price worldwide. Molecular markers have emerged as useful tools to assess the genetic diversity across crops. The study was conducted to explore genetic diversity of twenty lentil genotypes considering yield and yield attributing traits. Among all genotypes, BARI Masur-6, BARI Masur-7 followed by genotypes BD-3806 and BD-4090 showed the highest value of yield attributing traits therefore, these genotypes are considered as best performer. The results of cluster analysis based on the Ward’s method grouped the genotypes into three clusters and the genotypes of cluster III revealed the maximum value for yield per plants which indicated their importance in the selection for yield improvement program of lentil. Afterwards, 20 genotypes were evaluated through 7 sets of SSR primers to assess genetic diversity among the genotypes. Among them, four sets of primers viz., SSR 19, SSR 33, SSR 90 and SSR 213 showed high polymorphism which suggesting the greater genetic diversity in the genotypes. The unweighted pair group method of arithmetic mean (UPGMA) dendrogram based on Nei’s (1973) genetic distance led the genotypes into four major clusters which showed a bit deviation with the morphological cluster. The findings of this study will be very useful for selection of appropriate parents and the genetic understanding for the set up for future systematic lentil breeding programs.

Growing seedlings white poplar (populus alba l.) Based on the collection in vitro and evaluation of its cost

2016 ◽  
Vol 6 (1) ◽  
pp. 28-44 ◽  
Author(s):  
Табацкая ◽  
Tatyana Tabatskaya ◽  
Машкина ◽  
Olga Mashkina ◽  
Морковина ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Ex Situ Conservation ◽  
Hybrid Poplar ◽  
Ex Situ ◽  
Triploid Hybrid ◽  
Populus Alba ◽  
White Poplar ◽  
Nutrient Media ◽  
Axillary Meristems

New data on the effectiveness of using established in vitro growing number of lectures trudnorazmnozhaemyh triploid hybrid poplar ex situ conservation for white representatives of valuable genetic diversity and cultivation of planting mate-rial to create targeted plantations. A collection of clones over 20 years supported by Xia rare subculturing (based on the proliferation of axillary meristems) ste-sterile microplants (at intervals of every 5-6 months) to bezgormonal-nyh nutrient media under conditions in vitro.

scholarly journals Main and Interaction Effects of Factors on Softwood Cutting of White Poplar (Populus alba L.).

2007 ◽  
Vol 56 (1-6) ◽  
pp. 287-294 ◽  
Author(s):  
A. Harfouche ◽  
N. Baoune ◽  
H. Merazga
Keyword(s):  
Vegetative Propagation ◽  
Populus Alba ◽  
White Poplar ◽  
Important Species ◽  
Softwood Cutting ◽  
Individual Scale ◽  
The Individual ◽  
Rooting Response ◽  
Root Suckers ◽  
Selection Of

Abstract White poplar is an important species for reforesting riparian sites in Algeria, but efficient techniques are still to be perfected. The study investigates the feasibility of softwood cutting as a method of propagation of the species. Results showed that sand or gravel, used as substrate, do not determine significant differences in rooting percent, but exert an influence on the quality and vigour of roots of cuttings. The findings, also, suggest that softwood cutting of the species would be most efficient if root suckers and/or sticklings were the sources of cuttings. Moreover, summer is the best period for achieving good results, while IBA treatments would have little effect on the rooting response of cuttings. Significant stand and individual within stand variation in rooting response of cuttings was found. However, the low or moderate broad sense heritabilities suggest that microsite effects at the individual scale could influence significantly the results. Overall, the results of the investigations suggest that selection of stands and, individuals within stands with good rooting ability, together with taking care with respect to factors such as the period of cuttings collection and the sources of cuttings, could achieve real progress in vegetative propagation of white poplar in Algeria.

WINTER WHEAT DEVELOPED BY THE UDMURT RESEARCH INSTITUTE OF AGRICULTURE IN COMPETITIVE VARIETY TESTING

2020 ◽  
pp. 5-16
Author(s):  
I.V. TORBINA ◽  
◽  
I.R. FARDEYEVA ◽  
Keyword(s):  
Winter Wheat ◽  
Crop Rotation ◽  
Biological Characteristics ◽  
Commercial Use ◽  
Breeding Material ◽  
Rotation Pattern ◽  
Selection Of ◽  
Variety Testing ◽  
Made In ◽  
Research Institute

The paper assesses the promising varieties of winter wheat in a competitive variety test by the main economic and biological characteristics that determine the suitability of the variety for commercial use. The object of research was the authors’ own breeding material. The experiments on the selection of winter wheat were made in the experimental crop rotation pattern of the Institute.

On syntaxonomy of floodplain forests (Salicetea purpureae Moor 1958) of the Irtysh and Black Irtysh rivers

2005 ◽  
pp. 82-92
Author(s):  
G. S. Taran
Keyword(s):  
Populus Alba ◽  
Floodplain Forests ◽  
White Poplar ◽  
Salix Alba ◽  
River Floodplain ◽  
White Willow ◽  
Irtysh River

The paper characterizes poplar (Populus alba, P. nig­ra) and white willow (Salix alba) forests of the Irtysh and the Black Irtysh river floodplain, together with the silvery salt tree (Halimodendron halodendron) community of the Black Irtysh river floodplain. The Black Irtysh floodplain willow and poplar forests are separated into a new alliance, Rubio dolichophyllae—Populion albae Taran all. nov. (Salicetalia purpureae Moor 1958, Salicetea purpureae Moor 1958), which includes the associations Rubio dolichophyllae—Popu­letum albae Taran 1997 and Rubio dolichophyllae—Salicetum albae Taran ass. nov. The Halimodendron halodendron community is probably related to the class Nerio-Tamaricetea Br.-Bl. et de Bolós 1958. White poplar forests in the Irtysh R. upper reaches, described within the Semipalatinsk city limits, belong to the association Heracleo dissecti—Populetum albae Taran 1997 and its subassociation H. d. —P. a. lamietosum albi Taran 1997 (Equiseto hyemalis—Populion nigrae Taran 1997, Salicetalia purpureae Moor 1958).

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