scholarly journals SPECTRUM OF SOLUBLE PROTEINS OF SPRING WHEAT LEAVES FOR FOLIAR TREATMENT BY CHELATE MICROERTILER ZHUSS-2

2017 ◽  
Vol 12 (2) ◽  
pp. 34-37
Author(s):  
Валентина Пахомова ◽  
Valentina Pahomova ◽  
Насия Даминова ◽  
Nasiya Daminova ◽  
Наталья Кузнецова ◽  
...  
Keyword(s):  
Molecular Mass ◽  
Spring Wheat ◽  
Control Sample ◽  
Soluble Proteins ◽  
Single Treatment ◽  
Molecular Weights ◽  
Foliar Treatment ◽  
Quantitative Changes ◽  
Wheat Leaves

The spectrum of soluble proteins of spring wheat leaves was studied for foliar treatment with chelated copper, molybdenum-containing microfertilizer ZhUSS-2 (0.1% solution). Electrophoretically, polypeptides with a molecular mass of 13.2 - 145 kD were detected. The qualitative composition of soluble proteins in the leaves of spring wheat plants under the influence of foliar treatment during the vegetation did not change, however, their quantitative changes were observed. During a single treatment of spring wheat in the tillering phase, an increase in polypeptides with a molecular mass of 145 and 94 kD was recorded; the content of polypeptides with molecular weights of 13.2 - 66 kD decreased in comparison with the control sample. When the plants were sprayed twice in the tillering and tubing phases, the content of these polypeptides as well as polypeptides with molecular weights of 45 and 41 kD increased. The content of other detected polypeptides also decreased compared to the control variant. During a three-fold treatment of spring wheat plants in the tillering, tubing and blossoming-flowering phases with the ZhUSS-2 preparation, the number of all detected polypeptides increased in comparison with the control sample. Thus, the response of the metabolism of soluble proteins in the cells of spring wheat leaves to the action of foliar top dressing with copper, molybdenum-containing chelate microfertilizer of ZhUSS-2 brand in different phases of vegetation is different and depends on the dose of exposure. Particular attention should be paid to the role of two polypeptides in the treatment of vegetative plants by ZhUSS-2 M.M. 94 kD and, mainly, 145 kD. There is a reason to believe that copper molybdenum-containing chelate microfertilizer ZhUSS-2 exhibits the action of an abiogenic elicitor, which leads to activation of the gene apparatus of cells. The possible functional role of these proteins is discussed.

Exploring the role of identity fusion and group identification in predicting parochialism amongst Indonesian Islamic groups

2019 ◽  
Author(s):  
Christopher Michael Kavanagh ◽  
Susilo Wibisono ◽  
Rohan Kapitány ◽  
Whinda Yustisia ◽  
Idhamsyah Eka Putra ◽  
...  
Keyword(s):  
Group Identification ◽  
Control Sample ◽  
Theoretical Models ◽  
Religious Groups ◽  
Diverse Range ◽  
Beliefs And Practices ◽  
Identity Fusion ◽  
Beliefs And Practice ◽  
Fusion Theory

Indonesia is the most populous Islamic country and as such is host to a diverse range of Islamic beliefs and practices. Here we examine how the diversity of beliefs and practices among Indonesian Muslims relates to group bonding and parochialism. In particular, we examine the predictive power of two distinct types of group alignment, group identification and identity fusion, among individuals from three Sunni politico-religious groups - a fundamentalist group (PKS), a moderate group (NU), and a control sample of politically unaffiliated citizens. Fundamentalists were more fused to targets than moderates or citizens, but contrary to fusion theory, we found across all groups, that group identification (not fusion) better predicted parochialism, including willingness to carry out extreme pro-group actions. We discuss how religious beliefs and practice impact parochial attitudes, as well as the implications for theoretical models linking fusion to extreme behaviour.

scholarly journals CRISP2, CATSPER1 and PATE1 Expression in Human Asthenozoospermic Semen

Cells ◽  
2021 ◽  
Vol 10 (8) ◽  
pp. 1956
Author(s):  
Francesco Manfrevola ◽  
Bruno Ferraro ◽  
Carolina Sellitto ◽  
Domenico Rocco ◽  
Silvia Fasano ◽  
...  
Keyword(s):  
Sperm Motility ◽  
Molecular Mechanisms ◽  
Gradient Centrifugation ◽  
Circular Rnas ◽  
Amino Acid Supplementation ◽  
Differential Analysis ◽  
Mrna Targets ◽  
Motility Regulation ◽  
Quantitative Changes

The etiology of human asthenozoospermia is multifactorial. The need to unveil molecular mechanisms underlying this state of infertility is, thus, impelling. Circular RNAs (circRNAs) are involved in microRNA (miRNA) inhibition by a sponge activity to protect mRNA targets. All together they form the competitive endogenous RNA network (ceRNET). Recently, we have identified differentially expressed circRNAs (DE-circRNAs) in normozoospermic and asthenozoospermic patients, associated with high-quality (A-spermatozoa) and low-quality (B-spermatozoa) sperm. Here, we carried out a differential analysis of CRISP2, CATSPER1 and PATE1 mRNA expression in good quality (A-spermatozoa) and low quality (B-spermatozoa) sperm fractions collected from both normozoospermic volunteers and asthenozoospermic patients. These sperm fractions are usually separated on the basis of morphology and motility parameters by a density gradient centrifugation. B-spermatozoa showed low levels of mRNAs. Thus, we identified the possible ceRNET responsible for regulating their expression by focusing on circTRIM2, circEPS15 and circRERE. With the idea that motility perturbations could be rooted in quantitative changes of transcripts in sperm, we evaluated circRNA and mRNA modulation in A-spermatozoa and B-spermatozoa after an oral amino acid supplementation known to improve sperm motility. The profiles of CRISP2, CATSPER1 and PATE1 proteins in the same fractions of sperm well matched with the transcript levels. Our data may strengthen the role of circRNAs in asthenozoospermia and shed light on the molecular pathways linked to sperm motility regulation.

Bacteriophages immunomodulate the response of monocytes

2021 ◽  
pp. 153537022199515
Author(s):  
Lídia Perea ◽  
Lorena Rodríguez-Rubio ◽  
Juan C Nieto ◽  
Carlos Zamora ◽  
Elisabet Cantó ◽  
...  
Keyword(s):  
Cytokine Production ◽  
Statistical Significance ◽  
Control Sample ◽  
Polymyxin B ◽  
Dependent Manner ◽  
Direct Role ◽  
Gram Positive Bacteria ◽  
Hla Dr ◽  
Tnf Α

Bacteriophages are present in fluids from cirrhosis patients. However, their effect on the immune response is unknown. In this work, we explore the role of phages in the phenotype, function, and cytokine production of monocytes. We stimulated healthy monocytes with five different butanol-purified phage suspensions infective for Gram-negative and Gram-positive bacteria. We studied the expression of the monocyte markers involved in lipopolysaccharide recognition (LPS; CD14), antigen presentation (HLA-DR) and co-stimulation (CD86), and the concentration of induced cytokines (TNF-α, IFN-α, and IL-10) by phages. To confirm the direct role of phages without the interference of contaminating soluble LPS in phage suspensions, polymyxin B was added to the cell cultures. Phagocytosis experiments were assessed by flow cytometry using labeled phage suspensions. We observed that butanol-purified phages reduced the surface levels of CD14 and CD86 in monocytes and increased the secreted levels of TNF-α and IL-10 compared with the control sample containing only butanol buffer. All phage suspensions showed downregulation of HLA-DR expression but only Staphylococcus aureus phage contaminated with Escherichia coli reached statistical significance. The addition of polymyxin B did not restore the monocytic response induced by phages, suggesting that the effect was not caused by the presence of LPS. Monocytes were able to phagocyte phages in a dose- and time-dependent manner. To conclude, the phagocytosis of butanol-purified phages altered the phenotype and cytokine production of monocytes suggesting they become tolerogenic.

A metalloproteinase extracellularly released byCrithidia deanei

2003 ◽  
Vol 49 (10) ◽  
pp. 625-632 ◽  
Author(s):  
Claudia Masini d'Avila-Levy ◽  
Rodrigo F Souza ◽  
Rosana C Gomes ◽  
Alane B Vermelho ◽  
Marta H Branquinha
Keyword(s):  
Molecular Mass ◽  
Polyacrylamide Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
Gel Filtration ◽  
Residual Activity ◽  
Major Protein ◽  
Motile Cells ◽  
Sds Page ◽  
Triton X

Actively motile cells from a cured strain of Crithidia deanei released proteins in phosphate buffer (pH 7.4). The molecular mass of the released polypeptides, which included some proteinases, ranged from 19 to 116 kDa. One of the major protein bands was purified to homogeneity by a combination of anion-exchange and gel filtration chromatographs. The apparent molecular mass of this protein was estimated to be 62 kDa by sodium dodecyl sulfate – polyacrylamide gel electrophoresis (SDS–PAGE). The incorporation of gelatin into SDS–PAGE showed that the purified protein presented proteolytic activity in a position corresponding to a molecular mass of 60 kDa. The enzyme was optimally active at 37 °C and pH 6.0 and showed 25% of residual activity at 28 °C for 30 min. The proteinase was inhibited by 1,10-phenanthroline and EDTA, showing that it belonged to the metalloproteinase class. A polyclonal antibody to the leishmanial gp63 reacted strongly with the released C. deanei protease. After Triton X-114 extraction, an enzyme similar to the purified metalloproteinase was detected in aqueous and detergent-rich phases. The detection of an extracellular metalloproteinase produced by C. deanei and some other Crithidia species suggests a potential role of this released enzyme in substrate degradation that may be relevant to the survival of trypanosomatids in the host.Key words: endosymbiont, trypanosomatid, extracellular, proteinase.

Quantitative changes in blood enzymes (catalase, amylase, lipase) under the influence of artificial sun

1929 ◽  
Vol 25 (9) ◽  
pp. 932-937
Author(s):  
N. A. Diveeva
Keyword(s):  
Modern Medicine ◽  
Quantitative Changes

Modern medicine in its most important fields is under the sign of the study of enzymes. Ziber-Shumova, noting the all-encompassing role of enzymes in biology, says: We are not able to imagine, generally speaking, life without enzymatic processes. The concept of life is associated with the concept of a number of the most diverse enzymatic processes.

HLA-G Expression in the Skin of Patients with Systemic Sclerosis

2009 ◽  
Vol 36 (6) ◽  
pp. 1230-1234 ◽  
Author(s):  
ISABELA J. WASTOWSKI ◽  
PERCIVAL D. SAMPAIO-BARROS ◽  
ELIANE M.I. AMSTALDEN ◽  
GUSTAVO MARTELLI PALOMINO ◽  
JOÃO FRANCISCO MARQUES-NETO ◽  
...  
Keyword(s):  
Systemic Sclerosis ◽  
Control Sample ◽  
Healthy Controls ◽  
Skin Disorders ◽  
Disease Prognosis ◽  
Laboratory Variables ◽  
Skin Biopsies ◽  
Dermal Cells

Objective.To determine HLA-G expression in skin biopsies from patients with systemic sclerosis (SSc), and its association with epidemiological, clinical, and laboratory variables and survival.Methods.Paraffin-embedded skin biopsies obtained from 21 SSc patients (14 limited SSc, 7 diffuse SSc) and from 28 healthy controls were studied. HLA-G expression was evaluated by immunohistochemistry.Results.HLA-G molecules were detected in 57% of skin biopsies from patients with SSc (9 from limited SSc, 3 from diffuse SSc), whereas no control sample expressed HLA-G (p = 0.000004). In patients, HLA-G molecules were consistently observed within epidermal and some dermal cells. HLA-G expression was associated with a lower frequency of vascular cutaneous ulcers (p = 0.0004), telangiectasias (p = 0.008), and inflammatory polyarthralgia (p = 0.02). After a 15-year followup, SSc patients who exhibited HLA-G survived longer than patients who did not.Conclusion.HLA-G is expressed in skin biopsies from patients with SSc, and this is associated with a better disease prognosis. This suggests a modulatory role of HLA-G in SSc, as observed in other skin disorders.

The role of low-molecular-mass electrolyte drug substances in the modification of the chitosan matrix

2015 ◽  
Vol 57 (3) ◽  
pp. 244-251 ◽  
Author(s):  
E. I. Kulish ◽  
A. S. Shurshina ◽  
V. V. Chernova ◽  
V. P. Zakharov
Keyword(s):  
Molecular Mass ◽  
Chitosan Matrix ◽  
Drug Substances

Isolation of specific protease inhibitors from Neurospora crassa

1976 ◽  
Vol 54 (8) ◽  
pp. 699-703 ◽  
Author(s):  
Peter H. Yu ◽  
Maria R. Kula ◽  
Hsin Tsai
Keyword(s):  
Neurospora Crassa ◽  
Protease Inhibitors ◽  
Gel Filtration ◽  
Physiological Role ◽  
Exchange Chromatography ◽  
Proteinase K ◽  
Tryptophan Synthase ◽  
Molecular Weights ◽  
Specific Protease

Four natural protease inhibitors have been partially purified by heat treatment, ion-exchange chromatography and gel filtration from Neurospora crassa. The inhibitory activity has been estimated by measuring the inhibition of proteolysis of casein as well as by the protection of Neurospora tryptophan synthase from proteolytic inactivation. The inhibitors are all oligopeptides and possess molecular weights in the range 5000 – 24 000 and appear to be very specific to Neurospora proteases. They may be classified into two types. The first are specific to Neurospora alkaline protease and the second to acidic protease. None of them exhibited any effect on other proteases including trypsin, chymotrypsin, papain, pepsin, thermolysin, subtilisin and proteinase K. The possible physiological role of these inhibitors is discussed.

scholarly journals Yield of spring wheat due to lipid peroxidation under the influence of Bacillus oligonitrophilus bacterization

2020 ◽  
Author(s):  
V.M. Pakhomova ◽  
◽  
A.I. Daminova ◽  
A.Yu. Kozhevnikov ◽  
I.V. Galiyakhmetov ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Spring Wheat ◽  
Stem Elongation ◽  
Wheat Variety ◽  
Wheat Yield ◽  
Concentration Field ◽  
Biologically Active ◽  
Agroclimatic Zone ◽  
Antioxidant Mechanism ◽  
Foliar Treatment

The purpose of the article was to study the resistance of spring wheat under the influence of foliar treatment of plants with silicate bacteria according to lipid peroxidation (LP) and yield. The studies were conducted on spring wheat (variety ‘MiS’). The cultivation technology is typical wheat in this agroclimatic zone. The foliar treatment was carried out with a culture of bacteria Bacillus oligonitrophilus by spraying at the rate of 0.5 l/m2 of bacteria suspension containing 109 cells/ml (equally to 100% concentration). Field experiment design: option 1 – plants without treatment (control); option 2 – treatment with B. oligonitrophilus fertilizers at the stage of tillering; option 3 – treatment with B. oligonitrophilus fertilizers at the stage of tillering/stem elongation; option 4 – treatment with B. oligonitrophilus at the stage of tillering/stem elongation/heading. The activity of LP in wheat leaves was identified according to the content of malondialdehyde (MDA). The content of MDA was determined photometrically during interaction with thiobarbiturate. Wheat yield increase was observed in options 3 and 4 (by 11 and 15%, respectively). The content of MDA decreased in all phases of wheat vegetation in the same experimental options (by 14 and 21%, respectively), which indicated the antioxidant effect of the studied preparation. Thus, the bacterization of wheat with B. oligonitrophilus leads to the plant resistance increase. The protective effect is probably due to the action of a complex of their biologically active substances (metabolites) including the antioxidant mechanism of action.

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