scholarly journals Analysis of Igf2 and Igf2r Genes Expression by RT-PCR in Bovine Fetuses.

2000 ◽  
Vol 17 (3) ◽  
pp. 124-127
Author(s):  
Yosuke Kawase ◽  
Ayako Moriki ◽  
Yoshiaki Minato ◽  
Seizo Hamano ◽  
Kiyoshi Matsukawa ◽  
...  
Keyword(s):  
Rt Pcr ◽  
Genes Expression ◽  
Bovine Fetuses

The monocyte transcriptome during pregnancy in multiple sclerosis: prominent expression of the Fc-receptor CD64

2010 ◽  
Vol 17 (4) ◽  
pp. 389-396 ◽  
Author(s):  
Rinze F Neuteboom ◽  
Evert Verbraak ◽  
Annet F Wierenga-Wolf ◽  
Jane SA Voerman ◽  
Marjan van Meurs ◽  
...  
Keyword(s):  
Multiple Sclerosis ◽  
Cell Signalling ◽  
Immune Functions ◽  
Rt Pcr ◽  
Third Trimester ◽  
Fc Gamma Receptor ◽  
The Third ◽  
Gamma Receptor ◽  
Genes Expression ◽  
Biological Interest

Background: During the third trimester of pregnancy multiple sclerosis (MS) disease activity is reduced. It is not fully understood which factors mediate this disease amelioration.Objective: To study alterations of the monocyte transcriptome during pregnancy in MS patients, using a genomewide approach to identify differentially regulated genes.Methods: Women with MS and healthy controls were longitudinally studied, including a visit before pregnancy.Results: RNA-microarray analysis was performed in six patients. We found a significant increase of CD64 (Fc gamma receptor 1a, FcgR1a) during the third trimester compared with baseline, confirmed by RT-PCR in a group of ten patients. Analysis with Ingenuity software was performed using all genes expression of which was altered at least 1.5-fold in at least five out of six patients. Major networks that were altered during MS pregnancy were: cell-to-cell signalling and interaction, immune response, and cell signalling. From the genes selected for Ingenuity analysis, seven additional candidate genes, selected for their biological interest, were tested using RT-PCR in ten patients with MS and nine controls. We found an increased expression of JAK2 and STAT1 directly postpartum in patients with MS and in controls.Conclusion: The increased CD64 expression during pregnancy is indicative of enhanced innate immune functions.

Genes Expression of Key Enzymes in Phenylpropanes Metabolism Pathway in Cucumber with RT-PCR

2013 ◽  
Vol 746 ◽  
pp. 53-57 ◽  
Author(s):  
Yu Qiao ◽  
Xue Jiao Meng ◽  
Xiao Xia Jin ◽  
Guo Hua Ding
Keyword(s):  
Disease Resistance ◽  
Secondary Metabolism ◽  
Environmental Conditions ◽  
Phenylpropanoid Metabolism ◽  
Rt Pcr ◽  
Gene Expressions ◽  
Key Enzymes ◽  
Metabolism Pathway ◽  
Genes Expression ◽  
Higher Temperature

Phenylpropanoid metabolism is an important secondary metabolism pathway in plants. Lignin, as a secondary metabolite was produced by secondary metabolism pathway, plays important roles on disease resistance in plants. PAL, C4H, and CHS are key enzymes involved in the secondary metabolism pathway and critical on disease resistance. We hypothesize that the expression of key enzymes of secondary metabolism pathway will be affected by abnormal environmental conditions; therefore provide the resistance to severe environment for plant. In this study, we analysed the gene expressions of PAL, C4H, and CHS under higher temperature and infection of germ (Pseudoperonosporacubensis) in Cucumber JingChun No.4, which is highly resistant to downy mildew. The gene expressions were quantified by semi-quantitative RT-PCR. Our results showed that the expression of C4H was consistently higher and not affected by germ inoculation. However, the expressions of PAL and CHS were increased at 2 h and 25 h, respectively, after inoculating with germs. Interestingly, we found that the expressions of all these three genes were decreased with treatment of higher temperature. Our results demonstrated that PAL, C4H and CSH are important in secondary metabolism and affect the potential resistant ability of plant to various severe growing environments.

scholarly journals Involvement of JAK-STAT-SOCS3 Pathway in the Regression of Mice X-Zone of Adrenal Cortex

2021 ◽  
Vol 5 (Supplement_1) ◽  
pp. A70-A71
Author(s):  
Thais Barabba Auricino ◽  
Eduarda Correa Bento ◽  
Claudimara Ferini Pacicco Lotfi
Keyword(s):  
Adrenal Cortex ◽  
Lipid Droplets ◽  
Virgin Female ◽  
Differentiation Process ◽  
Rt Pcr ◽  
Male And Female ◽  
Abnormal Distribution ◽  
Corticosterone Secretion ◽  
Genes Expression ◽  
Hematoxylin Eosin

Abstract Previously, we showed that the histological markers of the mice X-zone of adrenal cortex were still present in adult male and female postpartum SF1/SOCS3KO mice. Abnormal distribution of lipid droplets along the adrenal cortex and reduced ACTH-induced corticosterone secretion were observed in SF1/SOCS3KO mice (1). Here we have examined the adrenals of the SF1/SOCS3KO male and virgin female at 3, 8, 15 and 30 weeks through morphological and molecular analysis. Hematoxylin-eosin stains showed X-zone retention in the SF1/SOCS3KO mice adrenals regardless of the postnatal age analyzed. CYP17A1-positive cells were immunolocalized in the X-zone of SF1/SOCS3KO mice that were confirmed by immunoblotting. A fetal adrenal enhancer (FAdE) and Pik3c2g and 20αHSD genes expression were analyzed by RT-PCR, and these genes were present in the male SF1/SOCS3KO mice up to the age of 8 and 15 weeks, but not in the control mice. Therefore, we showed retention of X-zone in the adrenal cortex of SOCS3KO mice up to the age of 30 week, which suggest the involvement of JAK/STAT/SOCS3 signaling pathway in the differentiation process of adrenal cortex. Reference: (1) Pedroso et al., J Endocrinol.2017 Dec;235(3):207–222.

scholarly journals Effect of Valproic Acid on miRNAs Affecting Histone Deacetylase in a Model of Anaplastic Thyroid Cancer

2021 ◽  
Author(s):  
Nur Selvi Günel ◽  
Nihal Birden ◽  
Cansu Çalışkan Kurt ◽  
Bakiye Göker Bağca ◽  
behrouz shademan ◽  
...  
Keyword(s):  
Valproic Acid ◽  
Thyroid Cancer ◽  
Histone Deacetylase ◽  
Expression Patterns ◽  
Endocrine System ◽  
Anaplastic Thyroid Cancer ◽  
Anaplastic Thyroid Carcinoma ◽  
Rt Pcr ◽  
Surgical Interventions ◽  
Genes Expression

Abstract Thyroid cancer is the most common malignant tumor of the endocrine system seen in the thyroid gland. More than 90% of thyroid cancers comprise papillary thyroid cancer (PTC) and follicular thyroid cancer (FTC). Although anaplastic thyroid carcinoma (ATC) accounts for less than 2% of thyroid cancer. But patients' lifespan after diagnosis is about 6 months. Surgical interventions, radioactive iodine use, and chemotherapy are not sufficient in the treatment of ATC, so alternative therapies are needed. The WST-1 assay test was performed to evaluate the anti-proliferative effects of Valproic acid (VPA). Also, Effect of VPA on miRNAs affecting histone deacetylase were determined by Quantitative RT-PCR. In BRAFV600E / mut SW1736 (SW1736) cell line IC50 dose for VPA found 1.6 mg/ml. in our study, the level of oncogenic genes expression in cells treated with VPA, including miR-184, miR-222-5p, miR-124-3p, and miR-328-3p, decreased. Also, the expression of tumor inhibitory genes is miR-323-5p, miR-182-5p, miR-138-5p, miR-217, miR-15a-5p, miR-29b-3p, miR-324-5p and miR-101- 5p increased significantly. VPA can ad-just countless gene expression patterns, including microRNAs (miRNAs), by targeting histone deacetylase (HDAC). We got very promising results with VPA.

scholarly journals Inhibitory AKT2 And Vegr2 Genes Expression of Avicennia Marina Using RT-PCR On Widr Cells

2020 ◽  
Vol 8 (1) ◽  
pp. 1-4
Author(s):  
Qurrohman, T ◽  
Hasibuan, P.A.Z. ◽  
Basyuni, M
Keyword(s):  
Polymerase Chain Reaction ◽  
Cancer Chemoprevention ◽  
Avicennia Marina ◽  
Hexane Extract ◽  
Rt Pcr ◽  
Chain Reaction ◽  
Genes Expression ◽  
Regulated Expression ◽  
Pcr Method ◽  
Polymerase Chain

Objectives: To evaluate the activities the anticancer of n-hexane extract of Avicennia marina leaves on WiDr cells in down-regulated of expression of Akt2 and VEGFR2 genes. Methods: Avicennia marina leaves  were dried and extracted with n-hexane, analyzed the down-regulated Akt 2 and VEGFR2 expression which was determined the Reverse Transcription Polymerase Chain Reaction (RT-PCR) method. Results:N-hexane extract of Avicennia marina, in which there were down regulated expression Akt 2 and VEGFR2 of 0.43 and 0.50 WiDr cells. N-hexane extract of mangrove leaves has cancer chemoprevention activity with down-regulated on WiDr cells. Conclusions: :N-hexane extract of Avicennia marina leaves had anticancer activity with down-regulated on WiDr cells, suggest that significantly inhibit the expression of Akt2 and VEGFR2 genes.    

P.1.13 Time-dependent alterations in genes expression in rat brain after administration of antidepressants — a gene miccroarray, RT-PCR study

2009 ◽  
Vol 19 ◽  
pp. S13-S13
Author(s):  
M. Gaska ◽  
M. Kusmider ◽  
J. Solich ◽  
P. Palach ◽  
M. Dziedzicka-Wasylewska
Keyword(s):  
Rat Brain ◽  
Time Dependent ◽  
Rt Pcr ◽  
Genes Expression

Genome Wide Identification, Phylogeny and Expression Profiling Analysis of Shattering genes in Rapeseed and Mustard Plants

2021 ◽  
Author(s):  
Mahideen Afridi ◽  
Khurshid Ahmad ◽  
Shahana Seher Malik ◽  
Nazia Rehman ◽  
Muhammad Yasin ◽  
...  
Keyword(s):  
Expression Patterns ◽  
Phylogenetic Study ◽  
Rt Pcr ◽  
Protein Motif ◽  
Genome Wide ◽  
Genes Expression ◽  
Model Plant Arabidopsis Thaliana ◽  
Complete Genome Analysis ◽  
Profiling Analysis ◽  
Plant Arabidopsis Thaliana

Abstract Background: Non-synchronized pods shattering in the Brassicaceae family bring upon huge yield losses around the world. The shattering process was validated to be controlled by eight different genes in the model plant Arabidopsis thaliana, including SHATTERPROOF1, SHATTERPROOF2, FRUITFULL, INDEHISCENT, ALCATRAZ, NAC, REPLUMLESS and POLYGlACTOURANAZE. To obtain gene family & examine their expression patterns into fresh & mature silique, then completed genome wide identification, characterization, and expression analysis of shattering genes in B. napus and B. juncea.Results: Complete genome analysis of B. napus and B. juncea revealed 32 shattering genes, which were identified and categorize based on protein motif structure, exon-intron organization and phylogeny. The phylogenetic study revealed that these shattering genes contain little duplications that were determined with a distinct chromosome number. Motifs of 32 shattering proteins were also observed where motifs 6 were found to be more conserved. A single motif was observed for other genes like BrnS7, BrnS8, BrjS23 and BrjS26. Comparative genomics for synteny analysis was performed that validated a conserved pattern of blocks among these cultivars. RT-PCR based expressions profile showed higher expression of shattering genes in B. juncea as compared to B. napus. FUL gene was expressed more in the mature silique. ALC gene was not expressed in the fresh silique of B. napus but highly expressed in the mature silique. Conclusion: This study authenticates that shattering genes exist in the local cultivars of Brassica. ALC exhibited strong expression in both the mature and fresh silique of B. juncea. Our results showed that shattering genes expression occurred more in B. juncea as compared to B. napus. It also contributes to the screening of more candidate gene for further investigation and characterization.

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