Análise do nicho trófico e da influência das precipitações no forrageamento de Melipona eburnea Friese (Apidae: Meliponina) criadas no Acre, Brasil

Resumo. O presente trabalho teve como objetivo identificar plantas utilizadas por Melipona eburnea Friese para coleta de pólen e verificar a influência das precipitações no forrageamento dessa espécie. Para isso, realizou-se duas coletas semanais, de outubro de 2015 a junho de 2016, em três colônias de M. eburnea. Abelhas operárias transportando cargas de pólen foram capturadas na entrada de suas colônias, utilizando-se rede entomológica. As cargas de pólen foram retiradas das corbículas das operárias com o auxílio de pinça e, posteriormente, levadas ao Laboratório para serem analisadas. Os grãos de pólen foram fotomicrografados e identificados através da comparação com o pólen das plantas em floração na área estudada. Os dados foram obtidos a partir do número de grãos de pólen nas amostras com a média de precipitação para cada mês. Identificou-se 44 espécies botânicas, distribuídas em 18 famílias, as principais foram: Fabaceae, Myrtaceae, Euphorbiaceae e Arecaceae. Foi observado que a maior quantidade de pólen fornecido para M. eburnea ocorreu no período de menor volume pluviométrico. M. eburnea apresenta comportamento generalista na coleta de recursos polínicos, alterando seu nicho trófico, de acordo com a intensidade de floração. Plantas nativas representam a principal fonte de pólen para M. eburnea.Analysis of the trophic niche and the influence of the precipitations on the foraging of Melipona eburnea Friese (Apidae: Meliponina) created in Acre, Brazil Abstract. The present work had as objective to identify plants used to collect pollen per Melipona eburnea Friese and to verify the influence of the precipitations in the foraging of this specie. For tath, two weekly collections were carried out, from October 2015 to June 2016, in three colonies of M. eburnea. Worker bees carrying pollen loads were captured at the entrance of their colonies, using an entomological net. The pollen loads were removed from the workers' corbulas with the aid of forceps and then taken to the laboratory for analysis. The pollen grains were photomicrographs and identified by comparison with the pollen of the flowering plants in the studied area. The data were obtained from the number of pollen grains in the samples with the average precipitation for each month. It was identified 44 pollen types distributed in 18 families, the main ones being: Fabaceae, Myrtaceae, Euphorbiaceae and Arecaceae. It was observed that the highest amount of pollen supplied to M. eburnea occurred in the period of lower rainfall volume. M. eburnea presents general behavior in the collection of pollinic resources. Native plants represent the main source of pollen for M. eburnea.

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Pollen harvest features of the Central Amazonian bee Scaptotrigona fulvicutis Moure 1964 (Apidae: Meliponinae), in Brazil

Acta Botanica Brasilica ◽

10.1590/s0102-33062007000100002 ◽

2007 ◽

Vol 21 (1) ◽

pp. 11-20 ◽

Cited By ~ 21

Author(s):

Antonio Carlos Marques-Souza ◽

Maria Lúcia Absy ◽

Warwick Estevam Kerr

Keyword(s):

Plant Species ◽

Secondary Forest ◽

Pollen Grains ◽

Fruit Trees ◽

Pollen Loads ◽

Pollen Types ◽

Large Groups

Over a twelve-month period, pollen loads transported by Scaptotrigona fulvicutis Moure 1964 were collected from the workers corbiculae right after the hive entrance closure in an area of old secondary forest mixed with some exotic fruit trees and ornamentals. Once the pollen grains were identified, their monthly frequency in the samples and grouping by botanical family established that Mimosaceae, Myrtaceae and Sapindaceae were the most frequently visited. The workers harvested the pollen from 97 plant species distributed in 73 genera and 36 families, mostly: Stryphnodendron guianense (Aubl.) Benth. in April (57,37%) and Schefflera morototoni (Aubl.) Frodin in May (54,73%). The harvested pollen types abundance matrix showed that there was little species dissimilarity between the months, which resulted in the formation of two large groups.

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Bumblebee pollination of understorey shrub species in a tropical montane forest in Costa Rica

Journal of Tropical Ecology ◽

10.1017/s0266467400001632 ◽

2000 ◽

Vol 16 (5) ◽

pp. 657-672 ◽

Cited By ~ 7

Author(s):

Renate A. Wesselingh ◽

Heino C. M. Burgers ◽

Hans C. M. Den Nijs

Keyword(s):

Costa Rica ◽

Seed Set ◽

Pollen Grains ◽

Montane Forest ◽

Shrub Species ◽

Pollen Loads ◽

Visitation Rates ◽

Pollen Types ◽

Bumblebee Pollination ◽

Fruit And Seed Set

This study addresses the pollination ecology of shrub species in an upper montane forest in Costa Rica, where the diversity of insect visitors is much below that of the well-studied tropical forests of lower elevations. Data are presented on visitation rates by the tropical bumblebee Bombus ephippiatus to flowers of three species, pollen loads on bumblebee bodies, and pollen loads on stigmas of two of the species. High visitation rates to flowers of Palicourea brenesii were found, a species which produces copious amounts of nectar. Visitation rates to nectarless Leandra subseriata and Deppea grandiflora were much lower. Conspecific pollen comprised on average 90% of the pollen load on stigmas of Leandra, while Palicourea stigmas carried only 26 to 55% conspecific pollen. With only two ovules per flower, fruit and seed set in Palicourea were not limited by a shortage of conspecific pollen. In Leandra, 20–25% of the flowers received too few conspecific pollen grains for maximum seed set among 440 ovules per flower. Individual bumblebees showed no constancy in plant choice, judging from the high number of different pollen types (6–12) on their bodies. Workers returned frequently to the same Palicourea patch without visiting nearby Palicourea plants, which suggests the use of foraging paths.

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Spectrum of Pollen Stored by Melipona mandacaia (Smith, 1863) (Hymenoptera: Apidae, Meliponini) in an Urban Arid Landscape

Sociobiology ◽

10.13102/sociobiology.v64i3.1257 ◽

2017 ◽

Vol 64 (3) ◽

pp. 284 ◽

Cited By ~ 1

Author(s):

Thiago Francisco de Souza Carneiro Neto ◽

Patrícia Luiza Oliveira-Rebouças ◽

Josineide Edinalva Pereira ◽

Poliana Martins Duarte ◽

Maria Herbênia Lima Cruz Santos ◽

...

Keyword(s):

Leucaena Leucocephala ◽

Significant Positive Correlation ◽

Pollen Grains ◽

Trophic Niche ◽

Mimosa Pudica ◽

Pollen Collection ◽

Accumulation Curve ◽

Analysis Of Similarity ◽

Pollen Types ◽

Abiotic Variables

The objective of this study was to identify the species of pollen supplying plants that constitute the trophic niche of Melipona mandacaia in an urban area in the Caatinga domain (a tropical arid landscape). The collection of pollen in the colonies was carried out every 15 days, from October 2014 to September 2015. The pollen was removed directly from the storage pots in three distinct colonies. A total of 24 samples were analyzed and compared with the reference pollen collection, pollen catalogs and specialized literature. For the quantitative analysis, at least 1000 pollen grains per sample were identified. A total of 39 pollen types were identified, distributed in 17 botanical families, being one an indeterminate type. The most represented family was the Fabaceae (n = 16). The most frequent types were Leucaena leucocephala, Mimosa pudica and Melochia sp. There was a significant positive correlation between temperature and the number of pollen types throughout the study. Relative humidity and rainfall were abiotic variables that did not present a significant correlation. The rarefaction curve showed that probably most of the pollen types collected by the bees studied were sampled, since the accumulation curve showed a progressive tendency to stabilization, indicating that there was sample adequacy of the pollen types. The analysis of similarity revealed a high sharing of pollen sources between colonies.

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Foraging preferences of the native stingless bee Melipona seminigra pernigra (Apidae: Meliponini) in campo rupestre on canga of Serra dos Carajás, southeastern Amazonia

Biota Neotropica ◽

10.1590/1676-0611-bn-2020-1004 ◽

2021 ◽

Vol 21 (1) ◽

Author(s):

José Tasso Felix Guimarães ◽

Luciano Costa ◽

Daniela Cristina Zappi ◽

Wilson Filgueira Batista Junior ◽

Karen da Silva Lopes ◽

...

Keyword(s):

Native Plants ◽

Pollen Grains ◽

Mining Area ◽

Stingless Bee ◽

Campo Rupestre ◽

Pollen Data ◽

Dry Period ◽

Foraging Preferences ◽

Significant Difference ◽

Pollen Types

Abstract: Honey pollen samples of Melipona seminigra pernigraMoure & Kerr 1950 sampled between 2017 and 2019 from experimental apiaries installed in campo rupestre on canga (CRC) vegetation of the Serra dos Carajás aimed to evaluated seasonal floral availability of undisturbed and mining-influenced areas. Around one hundred pollen types were identified mainly belonging to Fabaceae, Myrtaceae and Euphorbiaceae (31, 6 and 5 species, respectively). Mining area presented the highest pollen richness, almost twice those identified in the undisturbed areas. 80% of the pollen types are rare with concentrations ≤ 2,000 pollen grains/10 g, while the remaining were the most abundant, frequent and the primary bee sources. These latter correspond mostly to native plants species such as Tapirira guianensis Aubl., Protium spp., Aparisthmium cordatum (A.Juss.) Baill., Mimosa acutistipula var. ferrea Barneby, Periandra mediterranea (Vell.) Taub., Miconia spp., Pleroma carajasense K.Rocha, Myrcia splendens (Sw.) DC., Serjania spp. and Solanum crinitum Lam. All pollen types were identified during both seasons, but higher concentration values are related to the dry period (June-September). The statistical analysis of the pollen data indicated that there was no significant difference between undisturbed and mining-influenced areas, since primary bee sources of this study are widespread used in revegetation of mined areas.

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Pollen loads of eucalypt and other pollen types in birds in NW Spain

Data in Brief ◽

10.1016/j.dib.2015.09.012 ◽

2015 ◽

Vol 5 ◽

pp. 348-350

Author(s):

María Calviño-Cancela ◽

Max Neumann

Keyword(s):

Nw Spain ◽

Pollen Loads ◽

Pollen Types

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Pollen Taphonomy in a Canyon Stream

Quaternary Research ◽

10.1016/0033-5894(87)90006-8 ◽

1987 ◽

Vol 28 (3) ◽

pp. 393-406 ◽

Cited By ~ 51

Author(s):

Patricia L. Fall

Keyword(s):

Surface Soil ◽

Pollen Grains ◽

Close Correlation ◽

Soil Samples ◽

Alluvial Deposits ◽

Modern Pollen ◽

Pollen Types ◽

Pollen Rain ◽

Arid Steppe ◽

Turbulent Water

AbstractSurface soil samples from the forested Chuska Mountains to the arid steppe of the Chinle Valley, Northeastern Arizona, show close correlation between modern pollen rain and vegetation. In contrast, modern alluvium is dominated by Pinus pollen throughout the canyon; it reflects neither the surrounding floodplain nor plateau vegetation. Pollen in surface soils is deposited by wind; pollen grains in alluvium are deposited by a stream as sedimentary particles. Clay-size particles correlate significantly with Pinus, Quercus, and Populus pollen. These pollen types settle, as clay does, in slack water. Chenopodiaceae-Amaranthus, Artemisia, other Tubuliflorae, and indeterminate pollen types correlate with sand-size particles, and are deposited by more turbulent water. Fluctuating pollen frequencies in alluvial deposits are related to sedimentology and do not reflect the local or regional vegetation where the sediments were deposited. Alluvial pollen is unreliable for reconstruction of paleoenvironments.

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Airborne pollen observations using a multi-wavelength Raman polarization lidar in Finland: characterization of pure pollen types

10.5194/acp-2020-794 ◽

2020 ◽

Author(s):

Xiaoxia Shang ◽

Elina Giannakaki ◽

Stephanie Bohlmann ◽

Maria Filioglou ◽

Annika Saarto ◽

...

Keyword(s):

Optical Properties ◽

Airborne Pollen ◽

Pollen Grains ◽

Wavelength Dependence ◽

Depolarization Ratio ◽

Mean Values ◽

Pollen Types ◽

Pine Pollen ◽

Significant Wavelength ◽

532 Nm

Abstract. We present a novel algorithm for characterizing the optical properties of pure pollen particles, based on the depolarization values obtained in lidar measurements. The algorithm was first tested and validated through a simulator, and then applied to the lidar observations during a four-month pollen campaign from May to August 2016 at the European Aerosol Research Lidar Network (EARLINET) station in Kuopio (62°44′ N, 27°33′ E), in Eastern Finland. Twenty types of pollen were observed and identified from concurrent measurements with Burkard sampler; Birch (Betula), pine (Pinus), spruce (Picea) and nettle (Urtica) pollen were most abundant, contributing more than 90 % of total pollen load, regarding number concentrations. Mean values of lidar-derived optical properties in the pollen layer were retrieved for four intense pollination periods (IPPs). Lidar ratios at both 355 and 532 nm ranged from 55 to 70 sr for all pollen types, without significant wavelength-dependence. Enhanced depolarization ratio was found when there were pollen grains in the atmosphere, and even higher depolarization ratio (with mean values of 25 % or 14 %) was observed with presence of the more non-spherical spruce or pine pollen. The depolarization ratio at 532 nm of pure pollen particles was assessed, resulting to 24 ± 3 % and 36 ± 5 % for birch and pine pollen, respectively. Pollen optical properties at 1064 nm and 355 nm were also estimated. The backscatter-related Ångström exponent between 532 and 1064 nm was assessed as ~ 0.8 (~ 0.5) for pure birch (pine) pollen, thus the longer wavelength would be better choice to trace pollen in the air. The pollen depolarization ratio at 355 nm of 17 % and 30 % were found for birch and pine pollen, respectively. The depolarization values show a wavelength dependence for pollen. This can be the key parameter for pollen detection and characterization.

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A study on the pollen morphology of the genus Combretum Loefl. and its taxonomic significance

South Asian Journal of Experimental Biology ◽

10.38150/sajeb.6(4).p131-142 ◽

2016 ◽

Vol 6 (4) ◽

pp. 131-142

Author(s):

Gamal E. B. E.B. El Ghazali

Keyword(s):

Pollen Grains ◽

Polar View ◽

Taxonomic Revision ◽

Molecular Classification ◽

Ecological Indicator ◽

Wide Range ◽

Pollen Types ◽

Specific Variation ◽

Palynological Study

Pollen grains of 92 species (from 99 collections) belonging to the genus Com-bretum (Combretaceae) were examined by Light Microscope (LM) and Scan-ning Electron Microscope (SEM). These species are representatives of vari-ous taxa outlined in the latest taxonomic revision of the genus. The genus belongs to a tropical or subtropical family, frequently encountered in various Quaternary deposits and acts as ecological indicator in various habitats. Pol-len grains of all members of the genus Combretum examined are hetero-colpate (with three simple apertures alternating with three composite ones) and exhibited wide range of inter-specific variation with respect to polar axes (P), Equatorial diameters (E), P/E ratios, shape classes, shape in polar view, costae pori, vestibula, protrusion of endoapertures and exine sculptur-ings. Species delimitation in these nine characters is tabulated. Four shape classes, eight exine sculpturings and seven pollen types were recognized. A key for identification to these pollen types is presented and the species in-cluded in each of these types are also outlined. Previous macro-morphological and molecular classification of the genus were discussed in the light of the present palynological study.

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Sporoderm morphogenesis in Euphorbia obesa and Croton gratissimus

Bothalia ◽

10.4102/abc.v14i3/4.1253 ◽

1983 ◽

Vol 14 (3/4) ◽

pp. 849-856 ◽

Cited By ~ 7

Author(s):

M. L. Frean

Keyword(s):

Pollen Morphology ◽

Pollen Grains ◽

Physiological Adaptation ◽

Structure Characteristic ◽

Pollen Type ◽

Electron Microscope Level ◽

Pollen Characters ◽

Pollen Types ◽

Unisexual Flowers ◽

Taxonomic Grouping

Pollen grains of the Euphorbiaceae show a number of pollen types which can be clearly distinguished. Generally different genera are characterized by a specific pollen type. Euphorbia obesa Hook. f. and Croton gratissimus Burch, subsp. subgratissimus (Prain) Burtt Davy, represent two genera within the Crotonoideae with different morphology, each type characteristic for the respective genus. Taxonomically, the genus Euphorbia with apetalous flowers consisting of a naked pistil surrounded by several staminate flowers within a cyathium, is considered more advanced than the genus Croton. In Croton the inflorescence is a raceme with unisexual flowers. The floral whorls of the male show numerous anthers and both calyx and a showy corolla are present. Both genera are insect pollinated. In both Euphorbia obesa and Croton gratissimus the pollen wall in section shows columellae, a structure characteristic of angiosperms. However the present ontogenetic studies show that the formation of the columellae differs entirely in the two pollen types. The final stratification of the wall as well as the morphology of the grains differ and evaluation of the exine structure indicates that phylogenetically Croton pollen shows more advanced characters than Euphorbia — contradicting the floral phylogeny. This study conducted at light and electron microscope level compares the two pollen types morphologically and ontogenetically, concentrating mainly on the formation of the exine which is tectate-perforate in the prolate tricolpate grain of Euphorbia obesa and semi-tectate in the anaperturate, spheroidal grain of Croton gratissimus. The aim of the study was to evaluate the significance of pollen characters in taxonomic and phylogenetic relationships within the Euphorbiaceae. The differing pollen morphology which is related to the taxonomic grouping of tribes within the subfamily (Crotonoideae) emphasizes diversity, which may result from physiological adaptation. The study shows that the same functional end may well be achieved in different ways and this may be a factor underlying the diversity in the heterogeneous family Euphorbiaceae.

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Precise Pollen Grain Detection in Bright Field Microscopy Using Deep Learning Techniques

Sensors ◽

10.3390/s19163583 ◽

2019 ◽

Vol 19 (16) ◽

pp. 3583 ◽

Cited By ~ 3

Author(s):

Ramón Gallardo-Caballero ◽

Carlos J. García-Orellana ◽

Antonio García-Manso ◽

Horacio M. González-Velasco ◽

Rafael Tormo-Molina ◽

...

Keyword(s):

Network Architecture ◽

Detection System ◽

Pollen Grains ◽

Pollen Grain ◽

Average Value ◽

Pollen Types ◽

Pollen Concentrations ◽

Pollen Counting ◽

Estimation System ◽

3D Information

The determination of daily concentrations of atmospheric pollen is important in the medical and biological fields. Obtaining pollen concentrations is a complex and time-consuming task for specialized personnel. The automatic location of pollen grains is a handicap due to the high complexity of the images to be processed, with polymorphic and clumped pollen grains, dust, or debris. The purpose of this study is to analyze the feasibility of implementing a reliable pollen grain detection system based on a convolutional neural network architecture, which will be used later as a critical part of an automated pollen concentration estimation system. We used a training set of 251 videos to train our system. As the videos record the process of focusing the samples, this system makes use of the 3D information presented by several focal planes. Besides, a separate set of 135 videos (containing 1234 pollen grains of 11 pollen types) was used to evaluate detection performance. The results are promising in detection (98.54% of recall and 99.75% of precision) and location accuracy (0.89 IoU as the average value). These results suggest that this technique can provide a reliable basis for the development of an automated pollen counting system.

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