Antibiotic Susceptibility Pattern and Biofilm Production of Multidrug - Resistant Organisms (MDROs) Isolated from Suez - Canal University Hospitals

2018 ◽  
Vol 27 (4) ◽  
pp. 113-121
Author(s):  
Hashem , Asmaa A. ◽  
Taha , Samaa A. ◽  
Anani , Maha M.
Keyword(s):  
Antibiotic Susceptibility ◽  
Multidrug Resistant ◽  
Suez Canal ◽  
Susceptibility Pattern ◽  
University Hospitals ◽  
Antibiotic Susceptibility Pattern ◽  
Biofilm Production ◽  
Multidrug Resistant Organisms

scholarly journals Phenotypic and genotypic characterization of biofilm producing clinical coagulase negative staphylococci from Nepal and their antibiotic susceptibility pattern

2021 ◽  
Vol 20 (1) ◽  
Author(s):  
Sarita Manandhar ◽  
Anjana Singh ◽  
Ajit Varma ◽  
Shanti Pandey ◽  
Neeraj Shrivastava
Keyword(s):  
Tissue Culture ◽  
Congo Red ◽  
Antibiotic Susceptibility ◽  
Clinical Samples ◽  
Susceptibility Pattern ◽  
Coagulase Negative Staphylococci ◽  
Antibiotic Susceptibility Pattern ◽  
Biofilm Production ◽  
Culture Plate ◽  
Tissue Culture Plate

Abstract Background Coagulase-negative staphylococci (CNS) survive as commensals of skin, anterior nares and external canals of human and were regarded as non-infectious pathogens. However, they are emerging as a major cause of nosocomial infectious due to their ability to form biofilms and high resistance to several classes of antibiotics. This study examines the biofilm forming abilities of 214 clinical CNS isolates using phenotypic and genotypic methods, and determines their antibiotic susceptibility patterns. Methods A total of 214 clinical isolates collected from different clinical samples were identified as CNS and their antibiotic susceptibility determined by CLSI guidelines. The biofilm forming ability of all isolates was determined by three phenotypic methods; Congo red agar (CRA) method, tube adherence method (TM) and tissue culture plate (TCP) method and by genotypic method for the detection of icaAD genes. Results Among all the isolates, S. epidermidis (57.5%) was found the most frequently, followed by S. saprophyticus (18.7%), S. haemolyticus (11.2%), S. hominis (7%), and S. capitis (5.6%). Antibiotic susceptibility pattern demonstrated 91.6% isolates were resistant to penicillin and 66.8% to cefoxitin while 91.1% isolates were susceptible to chloramphenicol. Constitutive and inducible clindamycin resistant phenotype as measured by D-test was seen among 28% and 14.5% of isolates respectively. Tissue culture plate method detected biofilm production in 42.1% isolate followed by 31.8% through tube method while 20.1% isolates were found to produce slime in Congo red agar method. The genotypic assay revealed presence of icaA and icaD genes in 19.2% isolates. Conclusion The study shows a high prevalence of biofilm formation and inducible clindamycin resistance in CNS isolates, indicating the importance of in-vitro biofilm production test and D-test in routine laboratory diagnostics. Implementation of efficient diagnostic techniques for detection of biofilm production in clinical samples can help manage staphylococcal infections and minimize risks of treatment failures in hospitals.

scholarly journals Antibiotic Susceptibility Pattern of Bacterial Pathogens Isolated from Infected Lesions

2019 ◽  
Vol 16 (41) ◽  
pp. 446-451
Author(s):  
Jatan Bahadur Sherchan ◽  
Pranita Gurung
Keyword(s):  
Risk Factors ◽  
Wound Infection ◽  
Antibiotic Susceptibility ◽  
Multidrug Resistant ◽  
Resistant Bacteria ◽  
Microbiological Analysis ◽  
Susceptibility Pattern ◽  
Multidrug Resistant Bacteria ◽  
Antibiotic Susceptibility Pattern ◽  
Number Of Patients

Background: Wound infection with multidrug resistant bacteria along with risk factors is a major burden and challenge to the health care persons. This study focuses on antibiotic susceptibility pattern of bacterial isolates and risk factors of patients with infected wounds.Methods: This was a cross-sectional study conducted between November 2017 to June 2018 in Kathmandu University Hospital. Pus sample and wound swabs collected from patients during the study period were included. All microbiological processing were done following standard guidelines and patient’s information was collected following ethical guidelines.Results: Total number of patients observed for wound infection was 2,763. Pathogenic bacteria were detected in 252(9.12%) samples. 167(66.27%) were multidrug resistant. 118(46.82%) had risk factors. Among risk factors 14 had malignancy, 12 had diabetes, 32 were above age 60 without other risk factors, 45 received prior antibiotics and had critical illness and 15 were renal failure patients. Multidrug resistant bacteria was detected more among patients with risk factors 101(85.59%) in comparison to patients without risk factor 66(49.25%). When p value was calculated it was found significant. (p=<0.05).Conclusions: Significant amount of multidrug resistant bacteria were found in wounds of patients with risk factors. Prevention of wound infection by taking care of postoperative wounds, controlling and treating the risk factors, avoiding misuse of antibiotics and early microbiological analysis of infected wound might help to reduce the burden in healthcare centers.Keywords: Extended spectrum beta-lactamase; methicillin resistant staphylococcus aureus; multidrug resistant.

Sign in / Sign up

Export Citation Format

Share Document