Serum Activities of Tartrate-Resistant Acid Phosphatase and Bone Specific Alkaline Phosphatase as Indices of Bone Metabolism in the Cow

Abstract Context: Patients with congenital adrenal hyperplasia (CAH) receive glucocorticoids as replacement therapy. Glucocorticoid therapy is the most frequent cause of drug-induced osteoporosis. Objective: The objective of the study was to evaluate bone mineral density (BMD) and bone metabolism in CAH patients. Design: This was a cross-sectional observational study. Setting: The study was conducted at a referral center for pediatric endocrinology. Patients and Other Participants: Thirty young patients with the classical form of CAH (aged 16.4–29.7 yr) treated with glucocorticoid from diagnosis (duration of treatment 16.4–29.5 yr) and 138 healthy controls (aged 16.0–30.0 yr) were enrolled. Main Outcome Measures: BMD was measured in the lumbar spine and whole body by dual-energy x-ray absorptiometry. Bone formation and resorption rates were estimated by serum measurements of bone-specific alkaline phosphatase and C-terminal telopeptide of type I collagen, respectively. Results: CAH patients were shorter than controls (women −6.8 and men −13.3 cm). Therefore, several methods were used to account for the effect of this difference on bone measurements. Whole-body BMD measurements were significantly lower, compared with controls (P < 0.03), after controlling for height (on average −2.5% in females and −9.3% in male patients). No differences were found in lumbar spine measurements. Bone-specific alkaline phosphatase and C-terminal telopeptide of type I collagen serum concentrations were higher in CAH patients than control subjects (P < 0.04). BMD measurements and bone metabolism markers did not correlate with the actual glucocorticoid dose or mean dose over the previous 7 yr. Conclusions: Young adult patients with the classical form of CAH have decreased bone density values, compared with healthy controls. This may put them at risk of developing osteoporosis early in life.

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Indicators of bone metabolism in patients of psychiatric clinic on the background of dimephosphone intake

Kazan medical journal ◽

10.17750/kmj2016-669 ◽

2016 ◽

Vol 97 (5) ◽

pp. 669-673

Author(s):

R Yu Il’ina ◽

O O Pasynkova ◽

L R Mukhamedzhanova ◽

L E Ziganshina

Keyword(s):

Alkaline Phosphatase ◽

Acid Phosphatase ◽

Mental Disorders ◽

Bone Metabolism ◽

Phosphatase Activity ◽

Serum Calcium ◽

Alkaline Phosphatase Activity ◽

Psychiatric Patients ◽

Control Group ◽

Tartrate Resistant Acid Phosphatase

Aim. To study the indicators of bone metabolism in patients of psychiatric clinics on the background of dimephosphone oral intake.Methods. Research was conducted at the Republican Clinical Psychiatric Hospital named after V.M. Bekhterev (Kazan). 64 patients (36 men and 28 women) aged 45 to 68 years (mean age 56.8±6.9 years) with various forms of mental disorders taking psychotropic drugs for the period of 3 years and over were examined. Resorption markers were determined - D-pyridinoline in urine, serum tartrate-resistant acid phosphatase, alkaline phosphatase, and the content of total and ionized serum calcium. All patients were divided into two groups: those who received dimephosphone in the combined systemic therapy and those who did not. The average duration of the dimephosphone administration was 22±4.2 days, the patients undergone on average 2.6±1.44 courses of treatment during 6 months.Results. The activity of tartrate-resistant acid phosphatase in psychiatric patients was higher compared with those of the control group. The maximum value was registered in patients who were not taking dimephosphone. The alkaline phosphatase activity was the highest in mentally healthy patients of the control group. In psychiatric patients treated with dimephosphone, alkaline phosphatase activity was 20% lower compared with the control group. The minimum values of alkaline phosphatase were registered in patients with mental disorders not taking dimephosphone. When studying the concentration of total and ionized serum calcium there were no significant differences between patients of comparison group and psychiatric patients.Conclusion. Shift in processes of bone remodeling toward its resorption indicates a high risk of pathological fractures in psychiatric patients and the need for osteoporosis prevention; identified changes in biochemical markers of osteoporosis on the background of dimephosphone intake allow to recommend it for the treatment of diagnosed osteoporosis in a psychiatric hospital settings.

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Investigation of the effect of anti-epileptic drugs on bone metabolism using osteoprotegerin and bone-specific alkaline phosphatase: The direct effects of antiepileptic drugs on bone metabolism

Journal of Surgery and Medicine ◽

10.28982/josam.958297 ◽

2021 ◽

Vol 5 (9) ◽

pp. 974-977

Author(s):

Buket TUĞAN YILDIZ ◽

Tuba Tülay KOCA ◽

Muhammed SEYİTHANOĞLU ◽

Duygun ALTINTAŞ AYKAN

Keyword(s):

Alkaline Phosphatase ◽

Bone Metabolism ◽

Antiepileptic Drugs ◽

Direct Effects ◽

Specific Alkaline Phosphatase ◽

Bone Specific Alkaline Phosphatase

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Opposite correlation of 25-hydroxy-vitamin D- and 1,25-dihydroxy-vitamin D-metabolites with gestational age, bone- and lipid-biomarkers in pregnant women

Scientific Reports ◽

10.1038/s41598-021-81452-9 ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Oleg Tsuprykov ◽

Saban Elitok ◽

Claudia Buse ◽

Chang Chu ◽

Bernhard Karl Krämer ◽

...

Keyword(s):

Vitamin D ◽

Alkaline Phosphatase ◽

Pregnant Women ◽

Clinical Chemistry ◽

Water Soluble ◽

Target Cells ◽

Vitamin D Metabolites ◽

Specific Alkaline Phosphatase ◽

Bone Specific Alkaline Phosphatase ◽

Group B

Abstract25-Hydroxyvitamin D (25OHD) and 1,25-dihydroxyvitamin D (1,25(OH)2D) need to be bound to carrier proteins to be transported to their target cells. The majority of either 25OHD or 1,25(OH)2D is bound to vitamin D-binding protein (DBP), a smaller fraction is bound to albumin and only very small amounts of 25OHD or 1,25(OH)2D are free. Albumin-bound 25OHD or 1,25(OH)2D is relatively easily available after dissociation from albumin. Thus, the sum of free and albumin-bound forms is called bioavailable 25OHD and bioavailable 1,25(OH)2D. Total 25OHD and 1,25(OH)2D are defined as the sum of free, albumin-bound and DBP-bound 25OHD and 1,25(OH)2D, respectively. This cross-sectional study in 427 pregnant women compared the correlation of the six vitamin D compounds with biomarkers of bone health, lipid metabolism, kidney function, endocrine parameters, and group B water-soluble vitamins. Among the 25OHD metabolites analysed, total 1,25(OH)2D showed clearly the best correlation with calcium, bone-specific alkaline phosphatase, adiponectin, LDL, HDL, urea, thyroxine, and group B water-soluble vitamins. When comparing the three 25OHD metabolites, both free 25OHD and bioavailable 25OHD showed overall good correlations with calcium, bone-specific alkaline phosphatase, adiponectin, LDL, HDL, urea, thyroxine, triiodothyronine, and group B water-soluble vitamins, The correlations of 1,25(OH)2D and 25OHD metabolites went always in opposite directions. Only PTH correlates always inversely with all six vitamin D compounds. In conclusion, free 25(OH)D and bioavailable 25(OH)D are more precise determinants of the vitamin D status than total 25(OH)D in normal pregnancy, whereas total 1,25(OH)2D is superior to free and bioavailable 1,25(OH)2D. Except for PTH, correlations of 25(OH)D and 1,25(OH)2D metabolites with typical clinical chemistry readouts go in opposite directions.

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Acute Response of Sclerostin to Whole-body Vibration with Blood Flow Restriction

International Journal of Sports Medicine ◽

10.1055/a-1422-3376 ◽

2021 ◽

Author(s):

Kyle S Gapper ◽

Sally Stevens ◽

Rona Antoni ◽

Julie Hunt ◽

Sarah J Allison

Keyword(s):

Alkaline Phosphatase ◽

Blood Flow ◽

Whole Body Vibration ◽

Blood Flow Restriction ◽

Whole Body ◽

Specific Alkaline Phosphatase ◽

Body Vibration ◽

Bone Specific Alkaline Phosphatase ◽

Flow Restriction ◽

Acute Response

AbstractBlood flow restriction may augment the skeletal response to whole-body vibration. This study used a randomised, crossover design to investigate the acute response of serum sclerostin and bone turnover biomarkers to whole-body vibration with blood flow restriction. Ten healthy males (mean±standard deviation; age: 27±8 years) completed two experimental conditions separated by 7 days: (i) whole-body vibration (10 1-minute bouts of whole-body vibration with 30 s recovery) or (ii) whole-body vibration with lower-body blood flow restriction (10 cycles of 110 mmHg inflation with 30 s deflation during recovery). Fasting blood samples were obtained immediately before and immediately after exercise, then 1 hour, and 24 hours after exercise. Serum samples were analysed for sclerostin, cross-linked C-terminal telopeptide of type I collagen, and bone-specific alkaline phosphatase. There was a significant time × condition interaction for bone-specific alkaline phosphatase (p=0.003); bone-specific alkaline phosphatase values at 24 hours post-exercise were significantly higher following whole-body vibration compared to combined whole-body vibration and blood flow restriction (p=0.028). No significant time × condition interaction occurred for any other outcome measure (p>0.05). These findings suggest that a single session of whole-body vibration combined with blood flow restriction does not significantly affect serum sclerostin or bone turnover biomarkers.

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Bone-Derived Serum Enzymes and Bone Density in Perimenopausal Caucasian Women

Annals of Clinical Biochemistry International Journal of Laboratory Medicine ◽

10.1177/000456329303000214 ◽

1993 ◽

Vol 30 (2) ◽

pp. 191-194 ◽

Cited By ~ 4

Author(s):

J D Johnston ◽

S Koneru ◽

T Kuwana ◽

S B Rosalki

Keyword(s):

Alkaline Phosphatase ◽

Acid Phosphatase ◽

Bone Density ◽

Femoral Neck ◽

Serum Levels ◽

Bone Alkaline Phosphatase ◽

Serum Enzymes ◽

Tartrate Resistant Acid Phosphatase ◽

Vertebral Bone ◽

Caucasian Women

Serum levels of bone-origin alkaline phosphatase and of tartrate-resistant acid phosphatase were measured in Caucasian women aged 41–69 years who had volunteered for bone densitometry. Bone alkaline phosphatase and tartrate-resistant acid phosphatase were inversely correlated with vertebral bone density and with femoral neck bone density. Bone alkaline phosphatase and acid phosphatase were also significantly correlated, consistent with the concept of ‘coupling’ between osteoblast and osteoclast activity.

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