Isolation of <i>Salmonella</i> from Cecal Content and Ileocolic Lymph Nodes of Finishing Pigs

Verocytotoxin-producing Escherichia coli (VTEC) has emerged as a foodborne pathogen that can cause severe and potentially fatal illnesses, such as hemorrhagic colitis or the hemolytic uremic syndrome. In this study, 182 cattle at slaughter (119 dairy cows and 63 feedlot cattle) were randomly selected and tested for the presence of VTEC serogroups O26, O103, O111, O145, and O157 in their cecal content and lymphatic tissue (tonsils or mesenteric lymph nodes). A total of 364 samples were evaluated with an immunomagnetic separation technique followed by slide agglutination. Presumptive VTEC O26, O103, O111, O145, and O157 isolates were tested by Vero cell assay for verocytotoxin production and by multiplex PCR assay for the detection of vtx1, vtx2, eae, and E-hlyA genes. VTEC O157 was detected in 6 (3.3%) of 182 animals, and VTEC O26 was detected in 1 (0.5%) of 182 animals. No VTEC O103, VTEC O111, or VTEC O145 isolates were found in cattle feces, but one VTEC O91:H− vtx2+, eae−, E-hlyA+ strain nonspecifically cross-reacted with the VTEC O103 type. The prevalence of VTEC O157 in the lymphatic tissue of cattle was 1.1% in both tonsils (1 of 93 samples) and mesenteric lymph nodes (1 of 89 samples). Lymphatic tissue contamination was observed only in VTEC O157 intestinal carriers; two (33.3%) of six fecal carriers were simultaneously VTEC O157 lymphatic carriers. This finding suggests that VTEC O157 contamination of meat does not necessarily come from feces or the environment. No other VTEC serogroups were detected in the lymphatic tissue of slaughtered cattle.

Download Full-text

PSVI-19 The Association of Benzoic Acid and Essential Oils Increases the Cecal Biodiversity in Weaned Piglets and Improves Growth Performance in Finishing Pigs

Journal of Animal Science ◽

10.1093/jas/skab054.326 ◽

2021 ◽

Vol 99 (Supplement_1) ◽

pp. 200-200

Author(s):

Sudario Roberto Silva Junior ◽

Maíra Resende ◽

Rhuan F Chaves ◽

Athos Silveira Marques ◽

Pedro Massahiro de Matos Murata ◽

...

Keyword(s):

Benzoic Acid ◽

Essential Oils ◽

Growth Performance ◽

Block Design ◽

Average Daily Gain ◽

Antimicrobial Properties ◽

Basal Diet ◽

Finishing Pigs ◽

Cecal Content ◽

Weaned Piglets

Abstract Benzoic acid (BA) and essential oils (EO) have antimicrobial properties and can be used in antibiotic restriction situations. The objective of this study was to evaluate the effects of BA and EO on cecal content microbiota in weaned piglets and growth performance from the nursery to finishing phase. One hundred and twenty barrows were weaned at 23 days (6.40 ± 0.53 kg) and assigned into 3 treatments (10 replicates) in randomized block design: basal diet without additives (NC), basal diet with antibiotics (PC), and association of 0,3% benzoic acid and essential oil (BA+EO). Colistin sulphate (200 ppm) in the nursery diets and enramycin (10 and 5 ppm) in the growing and finishing diets were used in the PC treatment. The pigs were weighed 0, 42 and 132 days into the experiment. On day 9, one animal per pen (7 replicates) was euthanized and the cecal content was collected for microbiome analyses. Growth performance was analyzed by MIXED procedure (SAS, 2009) and the Tukey test was used to compare the means (P < 0.050). The averages for biodiversity between treatments were compared using the number of OTUs and the Kruskal Wallis test (P < 0.050), and the statistical program STAMP was used. In the microbiome analyses, the results of the taxonomic classification, by principal components analysis (PCA), showed a marked difference in bacterial communities per treatment and a significant increase in the number of operational taxonomic units (OTUs) was observed in the BA+EO group (P < 0.05). At 132 days, the final weight (P = 0.006) and total average daily gain (P = 0.028) of BA+EO and PC were higher than NC. In conclusion, supplementation with BA+EO increases biodiversity in cecal content in the post-weaning period, resulting in an improvement in the growth performance of finishing pigs.

Download Full-text

Simultaneous infections by different Salmonella strains in mesenteric lymph nodes of finishing pigs

BMC Veterinary Research ◽

10.1186/1746-6148-10-59 ◽

2014 ◽

Vol 10 (1) ◽

pp. 59 ◽

Cited By ~ 13

Author(s):

Victoria Garrido ◽

Samanta Sánchez ◽

Beatriz San Román ◽

Ana Zabalza-Baranguá ◽

Yasmin Díaz-Tendero ◽

...

Keyword(s):

Lymph Nodes ◽

Finishing Pigs ◽

Mesenteric Lymph Nodes ◽

Mesenteric Lymph

Download Full-text

Estimation of the Salmonella enterica prevalence in finishing swine

Epidemiology and Infection ◽

10.1017/s0950268803001249 ◽

2004 ◽

Vol 132 (1) ◽

pp. 127-135 ◽

Cited By ~ 40

Author(s):

H. S. HURD ◽

J. D. McKEAN ◽

R. D. GRIFFITH ◽

M. H. ROSTAGNO

Keyword(s):

Lymph Nodes ◽

Salmonella Enterica ◽

Faecal Sample ◽

Finishing Pigs ◽

Study Objective ◽

Prevalence Estimates ◽

Low Sensitivity ◽

Swine Herds ◽

On Farm ◽

Serum Elisa

The study objective was to evaluate three methods of Salmonella enterica prevalence estimation in swine herds (faecal culture, culture of abattoir-collected samples, and serum ELISA). From each of six swine herds, we necropsied approximately 100 finishing pigs (>70 kg); one-half on farm and the other half at the abattoir, after transport and approximately 2·5 h holding. We collected the same samples for S. enterica culture at both locations (1 g faecal, 10 g caecal contents, ileocaecal lymph nodes, superficial inguinal lymph nodes, 25 g of gluteal muscle for serum ELISA). On farm, the 1 g faecal sample only detected 13·3% (2/15) of all positive pigs necropsied on farm. However, with abattoir and on-farm results combined, the faecal sample detected 57·4% (74/129) of positive pigs. Abattoir-collected samples provided prevalence estimates much higher than on-farm collected samples (39·9 vs. 5·3%; P<0·001). This study shows that faecal samples have a low sensitivity for detecting infected pigs and that abattoir-collected samples overestimate the on-farm S. enterica prevalence. For most herds, serology overestimated the on-farm culture prevalence.

Download Full-text

Scanning electron microscopy of freeze-fractured prescapular lymph node of caprine

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100111331 ◽

1984 ◽

Vol 42 ◽

pp. 244-245

Author(s):

O. Faroon ◽

F. Al-Bagdadi ◽

T. G. Snider ◽

C. Titkemeyer

Keyword(s):

Lymph Node ◽

Lymph Nodes ◽

Lymphatic System ◽

Three Dimensional ◽

Meat Products ◽

The Body ◽

Morphological Data ◽

The World ◽

Cellular Constituent ◽

Scanning Electron

The lymphatic system is very important in the immunological activities of the body. Clinicians confirm the diagnosis of infectious diseases by palpating the involved cutaneous lymph node for changes in size, heat, and consistency. Clinical pathologists diagnose systemic diseases through biopsies of superficial lymph nodes. In many parts of the world the goat is considered as an important source of milk and meat products.The lymphatic system has been studied extensively. These studies lack precise information on the natural morphology of the lymph nodes and their vascular and cellular constituent. This is due to using improper technique for such studies. A few studies used the SEM, conducted by cutting the lymph node with a blade. The morphological data collected by this method are artificial and do not reflect the normal three dimensional surface of the examined area of the lymph node. SEM has been used to study the lymph vessels and lymph nodes of different animals. No information on the cutaneous lymph nodes of the goat has ever been collected using the scanning electron microscope.

Download Full-text

Microvasculature and structure of hemal lymph nodes in the wall of the rabbit bladder: a vascular corrosion casting and EM study

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100141743 ◽

1995 ◽

Vol 53 ◽

pp. 1074-1075

Author(s):

F.E. Hossler ◽

M.I. McKamey ◽

F.C. Monson

Keyword(s):

Lymph Nodes ◽

Light Microscopy ◽

Corrosion Casting ◽

Fixed Tissue ◽

Infusion Pressure ◽

India Ink ◽

Cacodylate Buffer ◽

Rabbit Bladder ◽

Lateral Walls ◽

Comprehensive Study

A comprehensive study of the microvasculature of the normal rabbit bladder, revealed unusual "capillary glomeruli" along the lateral walls. Here they are characterized as hemal lymph nodes using light microscopy, SEM, TEM, ink injection, and vascular casting.Bladders were perfused via a cannula placed in the abdominal aorta with either 2% glutaraldehyde in 0.1M cacodylate buffer (pH 7.4) for fixation, 10% India ink in 0.9% saline and 0.1M phosphate (pH 7.4) for vessel tracing, or resin (Mercoximethylmethacrylate: catalyst, 4:1:0.3; Ladd Research Industries) for vascular corrosion casting. Infusion pressure was 100mm Hg. Fixed tissue was sectioned from epon-araldyte resin, and stained with toluidine blue for light microscopy, and lead and uranium for TEM. Ink injected tissue was photographed directly from saline-filled bladders illuminated from below. Resin-filled tissue was macerated in 5% KOH and distilled water. Casts were critical point dried, sputter coated with goldpalladium, and examined by routine SEM at 10 KV.

Download Full-text