scholarly journals Screening for larvicidal activity of ethanolic and aqueous extracts of selected plants against Aedes aegypti and Aedes albopictus larvae

2016 ◽  
Vol 4 (2) ◽  
pp. 143-147
Author(s):  
Michael Russelle Alvarez ◽  
◽  
Francisco Heralde ◽  
Noel Quiming ◽  
◽  
...  
Keyword(s):  
Aedes Aegypti ◽  
Larvicidal Activity ◽  
Aedes Albopictus ◽  
Aqueous Extracts

Repellent and Larvicidal Activity of the Essential Oil From Eucalyptus nitens Against Aedes aegypti and Aedes albopictus (Diptera: Culicidae)

2017 ◽  
Vol 54 (3) ◽  
pp. 670-676 ◽  
Author(s):  
Agustín Alvarez Costa ◽  
Cecilia V. Naspi ◽  
Alejandro Lucia ◽  
Héctor M. Masuh
Keyword(s):  
Essential Oil ◽  
Aedes Aegypti ◽  
Larvicidal Activity ◽  
Aedes Albopictus ◽  
Eucalyptus Nitens

scholarly journals Identification of entomopathogenic nematodes and symbiotic bacteria from Nam Nao National Park in Thailand and larvicidal activity of symbiotic bacteria against Aedes aegypti and Aedes albopictus

PLoS ONE ◽  
2018 ◽  
Vol 13 (4) ◽  
pp. e0195681 ◽  
Author(s):  
Temsiri Yooyangket ◽  
Paramaporn Muangpat ◽  
Raxsina Polseela ◽  
Sarunporn Tandhavanant ◽  
Aunchalee Thanwisai ◽  
...  
Keyword(s):  
Aedes Aegypti ◽  
Larvicidal Activity ◽  
Aedes Albopictus ◽  
National Park ◽  
Entomopathogenic Nematodes ◽  
Symbiotic Bacteria

scholarly journals Larvicidal activity of Vietnamese Solanum nigrum on mosquitoes Aedes aegypti and Aedes albopictus (Diptera: Culicidae)

2020 ◽  
Vol 52 (1) ◽  
Author(s):  
T.D.H. Dinh ◽  
Q.T. Le ◽  
T.D. Nguyen ◽  
T.Q.T. Nguyen ◽  
A.S. Ho ◽  
...  
Keyword(s):  
Aedes Aegypti ◽  
Larvicidal Activity ◽  
Aedes Albopictus ◽  
Plant Extract ◽  
Ethanolic Extract ◽  
Laboratory Strain ◽  
Hemorrhagic Fever ◽  
Solanum Nigrum ◽  
Hot Water ◽  
Control Effort

A Vietnamese domestic plant namely Solanum nigrum (S. nigrum) was subjected to test for larvicidal activity on two majors Dengue hemorrhagic fever (DHF) vectors Aedes aegypti (Ae. aegypti) and Aedes albopictus (Ae. albopictus). The plant was processed to get infusions in hot water or extracted in ethanol. Laboratory and field larval strains of two Aedes species were exposed to the infusions and extract at increasing concentrations for one hour and followed-up intensively for up to 72 hours. The obtained results of bioassay showed larvicidal effects of extract on all mosquito strains. The effects on laboratory strain of Ae. aegypti larvae were correlated with infusions and extract concentrations. Chopped plant infusions in hot water indicated mortality up to 77.3% of larvae. Ground plant infusions killed all of exposed larvae at day 3 postexposure. Median lethal concentrations (LC50,s) of chopped and ground plant infusions were 10.25 and 7.54%, respectively. Ethanolic extract had very strong effect on experimental subjects. Within 72 hours, 100% of laboratory strain of Ae. aegypti larvae died after exposure to extract at 100 parts per million (ppm) or higher concentrations. Ethanolic plant extract showed similar larvicidal effect on field strains of Ae. aegypti and Ae. albopictus. The percentage mortality of field strains larvae reached 100% after exposure to 100 ppm of plant extract. At concentrations of 1000 ppm, 100% of exposed larvae died with 8 hours. LC50 on tested larvae was 25.07-33.60 ppm. Strong larvicidal activity of S. nigrum suggests the possible application in DHF vector control effort.

scholarly journals Larvicidal and Enzymatic Inhibition Effects of Annona muricata Seed Extract and Main Constituent Annonacin against Aedes aegypti and Aedes albopictus (Diptera: Culicidae)

2019 ◽  
Vol 12 (3) ◽  
pp. 112 ◽  
Author(s):  
Rodrigues ◽  
Silva ◽  
Pinto ◽  
Lima dos Santos ◽  
Carneiro de Freitas ◽  
...  
Keyword(s):  
Aedes Aegypti ◽  
Bioactive Compounds ◽  
Larvicidal Activity ◽  
Aedes Albopictus ◽  
New Products ◽  
Seed Extract ◽  
Annona Muricata ◽  
Main Constituent ◽  
Environmentally Sustainable ◽  
Lc50 And Lc90

The mosquitoes Aedes aegypti and Aedes albopictus are vectors of arboviruses that cause dengue, zika and chikungunya. Bioactive compounds from plants are environmentally sustainable alternatives to control these vectors and thus the arboviruses transmitted by them. The present study evaluated the larvicidal activity of an acetogenin-rich fraction (ACERF) and its main constituent annonacin obtained from Annona muricata seeds on Ae. aegypti and Ae. albopictus. The larvicidal assays were performed using different concentrations to calculate the LC50 and LC90 values observed 24 h after exposure to the treatment. Annonacin was more active against Ae. aegypti (LC50 2.65 μg·mL−1) in comparison with Ae. albopictus (LC50 8.34 μg·mL−1). In contrast, the acetogenin-rich fraction was more active against Ae. albopictus (LC50 3.41 μg·mL−1) than Ae. aegypti (LC50 12.41 μg·mL−1). ACERF and annonacin treated larvae of Ae. aegypti and Ae. albopictus showed significant differences in the inhibition of their metabolic enzymes when compared to untreated larvae. The results demonstrate the relevant larvicidal action of the acetogenin-rich fraction and annonacin showing the potential to develop new products for the control of Ae. aegypti and Ae. albopictus.

scholarly journals Larvicidal Activity in vivo of Ethanolic and Aqueous Extracts From Moringa (Moringa oleifera Lam.) on Aedes aegypti L. (Diptera: Culicidae)

2019 ◽  
Vol 11 (8) ◽  
pp. 129
Author(s):  
Antonio Carlos Leite Alves ◽  
Toshik Iarley da Silva ◽  
Francisco Roberto de Azevedo ◽  
Estelita Pereira Lima ◽  
Renata Rocha Virgulino ◽  
...  
Keyword(s):  
Aedes Aegypti ◽  
Larvicidal Activity ◽  
Moringa Oleifera ◽  
Chemical Constituents ◽  
Control Treatment ◽  
Control Group ◽  
Distilled Water ◽  
Aqueous Extracts ◽  
Extract Concentration

The insecticides properties of Moringa oleifera (moringa) were evaluated in Aedes aegypti larvae, throughout an entirely randomized trial, represented by ethanolic and aqueous extracts obtained from leaves, flowers, barks, seeds and moringa roots, besides the control group. Five batches of 10 in 3rd stadium larvae were distributed in distilled water, added an extract concentration of 50 mL L-1, and the control treatment (distilled water). The test reading was measured after 24, 48 and 72 hours after larvae exposure, were considered dead those who did not respond to a mechanical stimulation of a clamp. The seed ethanolic extract produced the best performance after 24 hours (34% mortality), but after 48 hours, the flower extract was the more potent (38% mortality). The largest larvicidal activity was observed with the extract concentration of 90 mL L-1. The results indicate that moringa has larvacides properties against Aedes, but its chemical constituents need to be isolated and tested separately to enhance your larvicidal activity.

scholarly journals PirAB Toxin from Photorhabdus asymbiotica as a Larvicide against Dengue Vectors

2009 ◽  
Vol 75 (13) ◽  
pp. 4627-4629 ◽  
Author(s):  
Arunee Ahantarig ◽  
Nantarat Chantawat ◽  
Nicholas R. Waterfield ◽  
Richard ffrench-Constant ◽  
Pattamaporn Kittayapong
Keyword(s):  
Biological Control ◽  
Aedes Aegypti ◽  
Larvicidal Activity ◽  
Aedes Albopictus ◽  
Consensus Sequence ◽  
Related Protein ◽  
Enterobacterial Repetitive Intergenic Consensus ◽  
Control Efficacy ◽  
Dengue Vectors

ABSTRACT We have evaluated Photorhabdus insect-related protein (Pir) from Photorhabdus asymbiotica against dengue vectors. PirAB shows larvicidal activity against both Aedes aegypti and Aedes albopictus larvae but did not affect the Mesocyclops thermocyclopoides predator. PirAB expressed the strongest toxicity compared to PirA, PirB, or the mixture of PirA plus PirB. Whether the presence of an enterobacterial repetitive intergenic consensus sequence in PirAB, but not in PirA, PirB, or the mixture of PirA plus PirB, has any impact on biological control efficacy needs further investigation.

scholarly journals Analysis of chemical compositions and larvicidal activity of nut extracts from Areca catechu Linn against Aedes (Diptera: Culicidae)

PLoS ONE ◽  
2021 ◽  
Vol 16 (11) ◽  
pp. e0260281
Author(s):  
Madhuri Bharathithasan ◽  
Darvin R. Ravindran ◽  
Dinesh Rajendran ◽  
Sim Ka Chun ◽  
S. A. Abbas ◽  
...  
Keyword(s):  
Aedes Aegypti ◽  
Larvicidal Activity ◽  
Aedes Albopictus ◽  
Chemical Compounds ◽  
Chemical Compositions ◽  
Dodecanoic Acid ◽  
Molecular Formula ◽  
Tetradecanoic Acid ◽  
Link Type ◽  
Areca Catechu

Background There is a growing need to use green alternative larvicidal control for Aedes larvae compared to chemical insecticides. Substantial reliance on chemical insecticides caused insecticide resistance in mosquito populations. Thus, research for alternate chemical compounds from natural products is necessary to control Aedes larvae. This study explores the analysis of chemical compositions from Areca catechu nut as a potential larvicide for Aedes (Diptera: Culicidae). Methods The Areca catechu nut collected from Ipoh, Perak, Malaysia was grounded into powder and used for Soxhlet extraction. The chemical analysis of the extracts and their structures were identified using the GCMS-QP2010 Ultra (Shimadzu) system. National Institute of Standards and Technology (NIST) Chemistry WebBook, Standard Reference Database 69 (https://webbook.nist.gov/chemistry/) and PubChem (https://pubchem.ncbi.nlm.nih.gov/), the two databases used to retrieve the synonyms, molecular formula, molecular weight, and 2-dimensional (2D) structure of chemical compounds. Next, following WHO procedures for larval bioassays, the extracts were used to asses larvicidal activity against early 4th instar larvae of Aedes aegypti and Aedes albopictus. Results The larvicidal activities were observed against early 4th stage larvae with different concentrations in the range from 200 mg/L to 1600 mg/L. The LC50 and LC95 of Aedes aegypti were 621 mg/L and 2264 mg/L respectively; whereas the LC50 and LC95 of Aedes albopictus were 636 mg/L and 2268 mg/L respectively. Mortality was not observed in the non-target organism test. The analysis using gas chromatography and mass spectrometer recovered several chemical compounds such as Arecaidine, Dodecanoic acid, Methyl tetradecanoate, Tetradecanoic acid <n->, and n-Hexadecanoic acid bioactive components. These chemical constituents were used as additive formulations in pesticides, pest control, insect repellent, and insecticidal agents. Conclusions Our study showed significant outcomes from the extract of Areca catechu nut and it deserves further investigation in relation to chemical components and larvicidal actions between different species of Aedes mosquitoes. Even though all these findings are fundamental, it may have some interesting potentials to be developed as natural bio-larvicidal products.

scholarly journals Larvicidal activity of Xenorhabdus and Photorhabdus bacteria against Aedes aegypti and Aedes albopictus

2018 ◽  
Vol 8 (1) ◽  
pp. 31 ◽  
Author(s):  
Apichat Vitta ◽  
Punnawat Thimpoo ◽  
Wipanee Meesil ◽  
Thatcha Yimthin ◽  
Chamaiporn Fukruksa ◽  
...  
Keyword(s):  
Aedes Aegypti ◽  
Larvicidal Activity ◽  
Aedes Albopictus
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