scholarly journals Porous Polycaprolactone Scaffold Engineered with Naringin Loaded Bovine Serum Albumin Nanoparticles for Bone Tissue Engineering

2017 ◽  
Vol 14 (4) ◽  
pp. 1355-1362
Author(s):  
Balraj Sundaram ◽  
M. C. John Milton
Keyword(s):  
Tissue Engineering ◽  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Bovine Serum ◽  
X Ray Diffraction ◽  
Engineering Applications ◽  
X Ray ◽  
Albumin Nanoparticles

ABSTRACT: A biodegradable three-dimensional scaffolds have gathered attention and are widely studied for bone tissue engineering applications. In the present study, porous polycaprolactone scaffold entrapped with naringin loaded bovine serum albumin nanoparticles (PS-N-BSANP) has been engineered. Further, the prepared nanoparticles and interconnected porous scaffolds were characterized by scanning electron microscopy, X-ray diffraction and fourier transform infrared spectroscopy analysis. X- ray diffraction showed amorphization of naringin in PS-N-BSANP. In addition, sustained naringin release profile was observed from PS-N-BSANP for 12 days which showed a cumulative release of 52.54 micromolar (µM). Furthermore, conditioned medium from PS-N-BSANP showed an increased calcium deposition and collagen matrix formation under osteogenic conditions with C3H10T1/2 cell line. These results suggest that PS-N-BSANP enhanced the osteogenic differentiation potential in bone tissue engineering applications due to the controlled release of naringin.

scholarly journals Novel Bovine Serum Albumin Protein Backbone Reassembly Study: Strongly Twisted β-Sheet Structure Promotion upon Interaction with GO-PAMAM

Polymers ◽  
2020 ◽  
Vol 12 (11) ◽  
pp. 2603
Author(s):  
Andra Mihaela Onaș ◽  
Iuliana Elena Bîru ◽  
Sorina Alexandra Gârea ◽  
Horia Iovu
Keyword(s):  
Secondary Structure ◽  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Infrared Spectrometry ◽  
X Ray Diffraction ◽  
Protein Backbone ◽  
Raman Spectrometry ◽  
X Ray ◽  
Β Sheet

This study investigates the formation of a graphene oxide-polyamidoamine dendrimer complex (GO-PAMAM) and its association and interaction with bovine serum albumin (BSA). Fourier-transform infrared spectrometry and X-ray photoelectron spectrometry indicated the formation of covalent linkage between the GO surface and PAMAM with 7.22% nitrogen content in the GO-PAMAM sample, and various interactions between BSA and GO-PAMAM, including π-π* interactions at 291.5 eV for the binding energy value. Thermogravimetric analysis highlighted the increasing thermal stability throughout the modification process, from 151 to 192 °C for the 10% weight loss temperature. Raman spectrometry and X-ray diffraction analysis were used in order to examine the complexes’ assembly, showing a prominent (0 0 2) lattice in GO-PAMAM. Dynamic light scattering tests proved the formation of stable graphenic and graphenic-protein aggregates. The secondary structure rearrangement of BSA after interaction with GO-PAMAM was investigated using circular dichroism spectroscopy. We have observed a shift from 10.9% β-sheet composition in native BSA to 64.9% β-sheet composition after the interaction with GO-PAMAM. This interaction promoted the rearrangement of the protein backbone, leading to strongly twisted β-sheet secondary structure architecture.

The Effect of Bovine Serum Albumin on the Crystalline Characteristics of the CaP Biomimetic Coating

2005 ◽  
Vol 288-289 ◽  
pp. 363-366 ◽  
Author(s):  
Yao Wu ◽  
Ji Yong Chen ◽  
Yu Mei Xiao ◽  
Bang Cheng Yang ◽  
Xing Dong Zhang
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Crystal Size ◽  
X Ray Diffraction ◽  
X Ray ◽  
Natural Bone ◽  
Biomimetic Coating ◽  
Crystallite Sizes ◽  
Compound Coating

It is very necessary to develop a real biomimetic compound coating of CaP with organic component and investigate quantitatively the effects of different bovine serum albumin (BSA) concentration on the crystallite properties of the coprecipitated CaP layer. Bioactivated Ti was immersed in Ca-P solution with different BSA contents to obtain different biomimetic coating. The coatings were analyzed with scanning electron microscopy (SEM) and X-ray diffraction (XRD). With the increase of BSA, the crystals on the coating grew more slowly but packed more closely. The preferential crystallographic direction of 002 of hydroxyapatite became less distinguishable and the crystallinity of the deposited hydroxyapatite decreased gradually. The crystallite sizes reduced with the addition of BSA proteins. Accordingly, when a certain content of BSA protein was added to the Ca-P solution, Ti surface would form a real biomimetic coating with the crystal size and crystallinity similar to the natural bone.

Hydroxyapatite/Biopolymers Composite Scaffolds for Bone Tissue Engineering

2011 ◽  
Vol 493-494 ◽  
pp. 826-831
Author(s):  
A.C.B.M. Fook ◽  
Thiago Bizerra Fideles ◽  
R.C. Barbosa ◽  
G.T.F.S. Furtado ◽  
G.Y.H. Sampaio ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Freeze Drying ◽  
Three Dimensional ◽  
Porous Scaffolds ◽  
X Ray Diffraction ◽  
X Ray ◽  
Interconnected Pores

The application of a hybrid composite consisting of biopolymer and calcium phosphate, similar morphology and properties of natural bone, may be a way to solve the problem of the fragility of ceramics without reducing its mechanical properties, retaining the properties of biocompatibility and high bioactivity. This work aims at the preparation and characterization of three-dimensional scaffolds composite HA / biopolymers (chitosan and gelatin). The freeze-drying technique was employed in this study to obtain these frameworks and partial results showed the effectiveness of this method. This involved the study of structural, chemical and morphological frameworks, in order to direct the research suggested the application. The X Ray Diffraction (XRD) and infrared spectroscopy and Fourier transform (FTIR) results confirmed the formation of hydroxyapatite (HA) phase and the presence of characteristic bands of HA and biopolymers in all compositions. The microstructure of the scaffolds study conducted by Scanning Electron Microscopy (SEM) revealed the formation of longitudinally oriented microchannels with interconnected pores. In all compositions the porous scaffolds showed varying sizes and mostly larger than 100μm, and is therefore considered materials with potential for application in bone tissue engineering.

scholarly journals Nanosized Mesoporous Bioactive Glass/Poly(lactic-co-glycolic Acid) Composite-Coated CaSiO3Scaffolds with Multifunctional Properties for Bone Tissue Engineering

2014 ◽  
Vol 2014 ◽  
pp. 1-12 ◽  
Author(s):  
Mengchao Shi ◽  
Dong Zhai ◽  
Lang Zhao ◽  
Chengtie Wu ◽  
Jiang Chang
Keyword(s):  
Drug Delivery ◽  
Tissue Engineering ◽  
Mechanical Strength ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Glycolic Acid ◽  
X Ray Diffraction ◽  
X Ray ◽  
Simulated Body Fluids ◽  
Mesoporous Bioactive Glass

It is of great importance to prepare multifunctional scaffolds combining good mechanical strength, bioactivity, and drug delivery ability for bone tissue engineering. In this study, nanosized mesoporous bioglass/poly(lactic-co-glycolic acid) composite-coated calcium silicate scaffolds, named NMBG-PLGA/CS, were successfully prepared. The morphology and structure of the prepared scaffolds were characterized by scanning electron microscopy and X-ray diffraction. The effects of NMBG on the apatite mineralization activity and mechanical strength of the scaffolds and the attachment, proliferation, and alkaline phosphatase activity of MC3T3 cells as well as drug ibuprofen delivery properties were systematically studied. Compared to pure CS scaffolds and PLGA/CS scaffolds, the prepared NMBG-PLGA/CS scaffolds had greatly improved apatite mineralization activity in simulated body fluids, much higher mechanical property, and supported the attachment of MC3T3 cells and enhanced the cell proliferation and ALP activity. Furthermore, the prepared NMBG-PLGA/CS scaffolds could be used for delivering ibuprofen with a sustained release profile. Our study suggests that the prepared NMBG-PLGA/CS scaffolds have improved physicochemical, biological, and drug-delivery property as compared to conventional CS scaffolds, indicating that the multifunctional property of the prepared scaffolds for the potential application of bone tissue engineering.

scholarly journals Development and Characterization of Cellulose/Iron Acetate Nanofibers for Bone Tissue Engineering Applications

Polymers ◽  
2021 ◽  
Vol 13 (8) ◽  
pp. 1339
Author(s):  
Hamouda M. Mousa ◽  
Kamal Hany Hussein ◽  
Mostafa M. Sayed ◽  
Mohamed K. Abd El-Rahman ◽  
Heung-Myong Woo
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Photoelectron Spectroscopy ◽  
Cell Attachment ◽  
Future Application ◽  
Apatite Formation ◽  
Gravimetric Analysis ◽  
Engineering Applications ◽  
X Ray

In tissue engineering, design of biomaterial with a micro/nano structure is an essential step to mimic extracellular matrix (ECM) and to enhance biomineralization as well as cell biocompatibility. Composite polymeric nanofiber with iron particles/ions has an important role in biomineralization and collagen synthesis for bone tissue engineering. Herein, we report development of polymeric cellulose acetate (CA) nanofibers (17 wt.%) and traces of iron acetates salt (0.5 wt.%) within a polymeric solution to form electrospinning nanofibers mats with iron nanoparticles for bone tissue engineering applications. The resulting mats were characterized using field emission scanning electron microscopy (FESEM), transmission electron microscope (TEM), Fourier transform infrared (FTIR), thermal gravimetric analysis (TGA), differential scanning calorimetry (DSC), X-ray diffraction (XRD), and X-ray photoelectron spectroscopy (XPS). The resulted morphology indicated that the average diameter of CA decreased after addition of iron from (395 ± 30) to (266 ± 19) nm and had dense fiber distributions that match those of native ECM. Moreover, addition of iron acetate to CA solution resulted in mats that are thermally stable. The initial decomposition temperature was 300 °C of CA/Fe mat > 270 °C of pure CA. Furthermore, a superior apatite formation resulted in a biomineralization test after 3 days of immersion in stimulated environmental condition. In vitro cell culture experiments demonstrated that the CA/Fe mat was biocompatible to human fetal-osteoblast cells (hFOB) with the ability to support the cell attachment and proliferation. These findings suggest that doping traces of iron acetate has a promising role in composite mats designed for bone tissue engineering as simple and economically nanoscale materials. Furthermore, these biomaterials can be used in a potential future application such as drug delivery, cancer treatment, and antibacterial materials.

Porous Calcium Sulfate/Hydroxyapatite Whiskers Scaffold for Bone Tissue Engineering

2013 ◽  
Vol 738 ◽  
pp. 38-41 ◽  
Author(s):  
Ting Ting Yan ◽  
Xiao Pei Wu ◽  
Yong Shun Cui ◽  
Qing Hua Chen ◽  
Zhong Da Yang
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Calcium Sulfate ◽  
Porous Scaffolds ◽  
X Ray Diffraction ◽  
Composite Scaffolds ◽  
X Ray ◽  
Testing Techniques ◽  
Controllable Degradation

Porous calcium sulfate/hydroxyapatite whiskers composites scaffold is possible to be used in bone tissue engineering, for its biocompatibility, controllable degradation, and good mechanical properties. In this study, porous calcium sulfate/hydroxyapatite whisker composite scaffolds were fabricated by adding pore-forming agent and were characterized. The characteristics of the porous scaffolds were assessed by means of scanning electron microscopy (SEM), X-ray diffraction (XRD) and immersing testing techniques. It is shown that the scaffolds prepared in this article have the porous structure with the content of CaSO4 and HA. The scaffolds prepared in this article have been confirmed to be ideally used as biodegradable implants.

scholarly journals SYNTHESIS AND CHARACTERIZATION OF COMPOSITES BASED ON HYDROXYAPATITE NANOPARTICLES AND CHITOSAN EXTRACTED FROM SHELLS OF THE FRESHWATER CRAB Potamon algeriense

2020 ◽  
Vol XXV ◽  
pp. 132-142
Author(s):  
Mohammed Lakrat ◽  
Soufiane Fadlaoui ◽  
Mohamed Aaddouz ◽  
Ouahid El Asri ◽  
Mohammed Melhaoui ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Biomedical Applications ◽  
Freshwater Crab ◽  
Hydroxyapatite Nanoparticles ◽  
X Ray Diffraction ◽  
X Ray ◽  
Nanocrystalline Hydroxyapatite

Nanocrystalline hydroxyapatite (n-HAp), which has low crystallinity, has attracted great attention due to its similarity to the inorganic part of human bone. Therefore, many studies have focused on creating new formulations combining n-HAp with some biopolymers, such as chitosan, in order to imitate biological bone tissue. The importance of chitosan and its derivatives in biomedical applications has grown significantly in the last three decades due to its biodegradability and renewable source. Besides, chitosan and its derivatives present excellent biocompatibility and biofunctionality, which make them promising materials in bone tissue engineering. In the present study, the chitosan was, first, extracted from the shell of the freshwater crab species Potamon algeriense following demineralization, deproteinization, decolouration (raw chitin) and deacetylation (chitosan) steps. Then, a novel composite based on n-HAp and extracted chitosan (CTS) with varying chitosan contents, from 5% to 20% (w/w), was synthesized and characterized for potential application in tissue regeneration. The obtained composites were characterized using X-ray diffraction, Fourier transform infrared spectroscopy and thermogravimetric analysis. The precipitated n-HAp/CTS nanocomposites similar to natural bone are promising composites for bone tissue engineering applications.

scholarly journals Cuttlefish Bone-Derived Biphasic Calcium Phosphate Scaffolds Coated with Sol-Gel Derived Bioactive Glass

2019 ◽  
Author(s):  
Ana S. Neto ◽  
Daniela Brazete ◽  
José M.F. Ferreira
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Calcium Phosphates ◽  
Sol Gel ◽  
X Ray Diffraction ◽  
Bioactive Glasses ◽  
X Ray ◽  
Hydrothermal Transformation

The combination of calcium phosphates (CaP) with bioactive glasses (BG) has received an increased interest in the field of bone tissue engineering. In the present work, biphasic calcium phosphates (BCP) obtained by hydrothermal transformation (HT) of cuttlefish bone (CB) were coated with a Sr-, Mg- and Zn-doped sol-gel derived BG. The scaffolds were characterized by X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR) and scanning electron microscopy (SEM). The initial CB structure was maintained after HT and the scaffold functionalization did not jeopardize the internal structure. The results of in vitro bio-mineralization after immersing the BG coated scaffolds in simulated body fluid (SBF) showed extensive formation of bone-like apatite onto the surface of the scaffolds. Overall, the functionalized CB derived BCP scaffolds revealed promising properties for their use in bone tissue engineering field.

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