scholarly journals Chemical Composition and Bioactivity of the Essential Oils Derived from Artemisia Annua Against the Red Flour Beetle

2019 ◽  
Vol 16 (2) ◽  
pp. 463-476
Author(s):  
Mamata Deb ◽  
Dolly Kumar

Tribolium casteneum is the most resistant stored grains pest causing more than 40% grains loss per year. Replacement of the conventional fumigants with an eco-friendly alternative seemed an intelligent move to control the pest which has inclined global research towards the efficacy of pesticidal plants. In the race of finding a better insecticidal candidate, we focused on to the chemical composition of the essential oils (EO) derived with polar and non-polar solvents from Artemisia annua and their possible bioactivity against the pest species. GC-MS analyses of Chloroform and n-Hexane derived EOs showed the dominance of Oxygenated Sesquiterpene in the extract. Adults were found more vulnerable to n-Hexane EO (LD50= 0.71 mg adultˉ1) than to chloroform derived EO (LD50= 0.97 mg adultˉ1) in contact toxicity assays. In the fumigant bioassay both the adults and larvae were found susceptible towards n-Hexane EO with LD50 0.66 & 0.53 mg L airˉ1 respectively. Evaluation of the biomolecular profile of adults and larvae at their lethal doses to understand the molecular mechanism underlying oxidative stress has shown significant downfall (p˂0.01) in the activities of protein, AChE, GST, GSH whereas up regulation of LPO was distinctly marked. The basic knowledge of employing potential solvent in eluting EOs of A. annua would prove to be an efficient environmental friendly management tool against T. casteneum.

2014 ◽  
Vol 2014 ◽  
pp. 1-6 ◽  
Author(s):  
Asgar Ebadollahi ◽  
Jalal Jalali Sendi ◽  
Alireza Aliakbar ◽  
Jabraeil Razmjou

Utilization of synthetic acaricides causes negative side-effects on nontarget organisms and environment and most of the mite species such as two spotted spider mite,Tetranychus urticaeKoch, are becoming resistant to these chemicals. In the present study, essential oils of fennel,Foeniculum vulgareMill., and lavender,Lavandula angustifoliaMiller, were hydrodistilled using Clevenger apparatus and chemical composition of these oils was analyzed by GC-MS. Anethole (46.73%), limonene (13.65%), andα-fenchone (8.27%) in the fennel essential oil and linalool (28.63%), 1,8-cineole (18.65%), and 1-borneol (15.94%) in the lavender essential oil were found as main components. Contact and fumigant toxicity of essential oils was assessed against adult females ofT. urticaeafter 24 h exposure time. The essential oils revealed strong toxicity in both contact and fumigant bioassays and the activity dependeds on essential oil concentrations. Lethal concentration 50% for the population of mite (LC50) was found as 0.557% (0.445–0.716) and 0.792% (0.598–1.091) in the contact toxicity and 1.876 μL/L air (1.786–1.982) and 1.971 μL/L air (1.628–2.478) in the fumigant toxicity for fennel and lavender oils, respectively. Results indicated thatF. vulgareandL. angustifoliaessential oils might be useful for managing of two spotted spider mite,T. urticae.


2019 ◽  
Vol 9 (4) ◽  
pp. 148-156
Author(s):  
Reguig M’hamed dit Salah Dine ◽  
Labani Abdelrahamene ◽  
Terres Mohamed ◽  
Mellal Tahar

Chemical control using synthetic products against pests of stored foods converted with restrictions such as the toxicity of living things and ecological risks. Today the world opinion is oriented towards a new fight against pests, among the alternative strategy, the use of natural insecticides of plant origin. This work aims to evaluate the insecticide effect of essential oils of Rosmarinus eriocalyx leaves against a wheat pest stored Sitophilus granarius and the evaluation of their antioxidant activity in vitro. The insecticide activity of essential oils of this plant was assessed by the filter paper contact test method and the repulsion test by the method of preference and determination of lethal doses DL50 and DL90, and the percentage of repulsion, measurement of anti-radical activity by trapping free radical DPPH and determining EC50. With regard to the chemical composition of the volatile oils of R. eriocalyx, 1.8-cineol (48.47%), camphor (17.04%), 'pinene' (7.58%), 'pinene' (6.99%) were the main compounds, the results showed that the essential oil of the R.eriocalyx leaves has a very high rate of repulsion PR= 96.25% at the dose of 20 µl/ml after 120 minutes of exposure, the mortality rates of the wheat weevil in the toxicity tests have increased with increased doses and over time. lethal doses for the dose 20 µl/ml has 50% DL50 = 0.306 µl/cm2 and 90% DL90 = 2.513 µl/cm2 for 72 hours of exposure, , in the test system DPPH one determines the trapping activity of radical free of essential oil 23.794% ± 2.908% EC50 = 8.585 ± 2,608 mg/ml. The activity of synthetic antioxidants of gallic acid, quercetin and ascorbic acid were also determined in parallel experiments as positive control. The essential oils of R.eriocalyx leaves can be used as a natural alternative to synthetic insecticides to control pests of stored foodstuffs.


Molecules ◽  
2021 ◽  
Vol 26 (21) ◽  
pp. 6698
Author(s):  
Arunaksharan Narayanankutty ◽  
Aswathi Moothakoottil Kuttithodi ◽  
Ahmed Alfarhan ◽  
Rajakrishnan Rajagopal ◽  
Damia Barcelo

Essential oils are biologically and environmentally safe pesticidal compounds yielded from aromatic plants. Spices are important sources of essential oils, and they are widely used in the medicine, food, and various other industries. Among the different spices, Allspice (Pimenta dioica) is underexplored in terms of its biological efficacy and a limited number of studies are available on the chemical composition of Allspice essential oil (AEO); thus, the present study evaluated the larvicidal property, the repellency, and the fumigant toxicity against common pests of stored products of AEO. AEO was found to inhibit the survival of larvae of such vectors as Aedis, Culex, and Armigeres species. Further, AEO was found to exert repellant effects against the pests of such stored products as Sitophilus, Callosobruchus, and Tribolium. Similarly, the fumigant toxicity was found to be high for AEO against these species. The contact toxicity of AEO was high against Sitophilus and Callosobruchus. Apart from that, the essential oil was found to be safe against a non-target organism (guppy fishes) and was found to be non-genotoxic in an Allium cepa model. Overall, the results of the present study indicate that the essential oil from Allspice could be used as an environmentally safe larvicidal and biopesticidal compound.


2021 ◽  
Vol 13 (4) ◽  
pp. 1180-1186
Author(s):  
S. Aisvarya ◽  
M. Kalyanasundaram ◽  
M. Kannan ◽  
A. Lakshmanan ◽  
T. Srinivasan

Sitophilus oryzae L. (Curculionidae; Coleoptera) is considered to be a serious internal feeder of stored cereals. The use of insecticides results in the development of resistance among the pests and residues in the produce. Diatomaceous Earth (DE) is from a natural source, environment-friendly, safe to humans and natural enemies. In addition, it is highly effective against a wide range of stored pest species and has no toxic residues on the treated seeds. The promising alternative to synthetic insecticides is the application of DE in storage pest management under physical control. With this background, the present study was aimed to find the efficacy of DE against rice weevil, S. oryzae L. and their effect on the agro-morphological characters of maize (Zea mays L.) seeds. Contact toxicity bioassays were carried out with different concentrations of DE against S. oryzae. The results of the bioassay studies revealed LD50 at the concentration of 1.27 mg/100 gm of maize seeds. Further, 100 per cent mortality was achieved at the dose of 15 mg/100 gm of maize seeds within six days of exposure. The effect of DE on the germination provided a significant increase in germinability of maize seeds (LD50= 94%, LD95= 98% and control= 96%). DE at the concentration of LD95 had a beneficial effect on the seedling parameters, especially germination% (98%) and seedling length (53.02 cm) of maize. The present study concluded that DE could be effectively utilised as an alternative management tool to chemical insecticides in the management of rice weevil under storage conditions.


2019 ◽  
Vol 20 (2) ◽  
pp. 338-343 ◽  
Author(s):  
SHAHLA AMINI ◽  
MAJID GHORBANI NOHOOJI ◽  
MOUSA KHANI ◽  
MOHAMMAD REZA LABBAFI ◽  
FARAHNAZ KHALIGHI-SIGAROODI

Amini S, Nohooji MG, Khani M, Labbafi MR, Khalighi-Sigaroodi f. 2019. Biological activity of some essential oil constituents in four Nepeta L. species against Sitophilus oryzae L.. Biodiversitas 20: 338-343. Recently, there has been a growing interest in research concerning the possible use of plant extracts as alternatives to synthetic insecticides. Essential oils are among the best-known natural substances with insecticidal properties. These compounds may act as fumigants, contact insecticides, repellents, antifeedants and may affect growth and development of the insects. In an attempt to find a natural and inexpensive method for the control of stored-product pests, contact toxicity of essential oils of Nepeta cataria, Nepeta pogonosperma, Nepeta glomerulosa. and Nepeta binaloudensis. were investigated on adult insects of Sitophilus oryzae L. (Coleoptera, Curculionidae) in vitro condition. Chemical Composition, repellency and fumigant toxicity of the essential oils were investigated. Chemical composition of the essential oils of above plants were identified by GC-MS. 4aα,7α,7aβ-nepetalactone (82.74 %) was major component of N. cataria and also different isomers of nepetalactone were among the major compounds in N. Pogonosperma and N. binaloudensis oils. In fumigants bioassay, N. glomerulosa (124.318μL/L air) showed the highest toxicity against S. oryzae adults, followed by N.pogonosperma (150.49μL/L air) and N. cataria (152.630μL/L air), respectively. Also, the S. oryzae was repelled by N. cataria (91.61%) and N. binaloudensis (91.50 %), respectively.


2019 ◽  
Vol 37 (No. 2) ◽  
pp. 81-92 ◽  
Author(s):  
Enis Ben Bnina ◽  
Hafedh Hajlaoui ◽  
Ikbal Chaieb ◽  
Moncef Ben Said ◽  
Hichem Ben Jannet

The chemical composition of volatile fractions from leaves, flowers and peels of Citrus aurantium growing in Tunisia obtained by hydrodistillation were analysed using GC and GC-MS. Furthermore, the isolated essential oils were evaluated for their in vitro antimicrobial activity against eight bacteria, eight phytopathogenic and nine human pathogenic fungi. The essential oils from peels and its main compound limonene have been found to possess strong contact toxicity against four storage-grain insects. The highest mortality rate was observed when the essential oil was applied against Cryptolestes ferrugineus, Liposcelis bostrychophila and Tribolium castaneum.


Planta Medica ◽  
2008 ◽  
Vol 74 (09) ◽  
Author(s):  
T Milošević ◽  
N Nićiforović ◽  
V Mihailović ◽  
S Solujić ◽  
N Vuković

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