scholarly journals GC-MS Profiling and Antifungal Activity of Secondary Metabolite from Endophytic Fungus of Giloy

2021 ◽  
Vol 18 (4) ◽  
pp. 651-659
Author(s):  
Akanksha Raj Sriwastava ◽  
Vivek Srivastava
Keyword(s):  
Secondary Metabolites ◽  
Secondary Metabolite ◽  
Endophytic Fungus ◽  
Liquid Extraction ◽  
Tinospora Cordifolia ◽  
Surface Sterilization ◽  
Research Fields ◽  
Liquid Liquid Extraction ◽  
Antifungal Potential ◽  
Bioactive Potential

The endophytic microbiota is considered to be one of the consistent and noble souce of potential and unique natural amalgams. These natural amalgams carry diverse pharmaceutical significance which the reason for their importance among research fields. The diversity of plants carries much more diversity of the endophytes as their mutual parts where both are benefited from each other. The current work deals with the isolation of the endophytic fungus from Tinospora cordifolia, for which the leaves were used after the surface sterilization, followed by the production of secondary metabolite by the endophytic isolates through submerged fermentation technique. The produced metabolite was extracted by liquid-liquid extraction technique, which was further used for evaluating its antifungal potential against Candida albicans and the obtained results show their considerable potential. The GC-MS profiling of secondary metabolite was conducted to determine the presence of some bioactive compounds in them, and as a result, some potential compounds detected are Levoglucosenone, Silanediol, Nonane, D-Allose, 5-Hydroxymethylfurfural. Since these compounds are biologically important in various aspects which gives the diversified application to the secondary metabolites. The study concludes the potential of secondary metabolites from endophytic fungus of Tinospora cordifolia and further investigation can be approached on determining the same from other plants, and also evaluating another bioactive potential of secondary metabolites.

scholarly journals Solvent extraction and spectroscopy identification of bioactive compounds from medicinal shrub Tamarix gallica

2020 ◽  
Vol 10 (11) ◽  
pp. 456
Author(s):  
Moussa Mohammed Elamin
Keyword(s):  
Solvent Extraction ◽  
Phenolic Compounds ◽  
Secondary Metabolites ◽  
Bioactive Compounds ◽  
Liquid Extraction ◽  
Spectroscopy Analysis ◽  
Phytochemical Study ◽  
Liquid Liquid Extraction ◽  
Tamarix Gallica

This research is interested in chemical valorization of the medicinal shrub called Tamarix gallica. The phytochemical study of the extracts of this plant showed that it is rich in phenolic compounds especially the flavonoids. The liquid-liquid extraction by n-butanol and acetate of ethyl of the leaves allowed to us the extraction of the secondary metabolites of class of the flavonoids.The identification of the isolated flavonoids is made by the spectroscopy methods: UV, IR, NMRH, from where we extracted some structures of flavonols class. Keywords: Tamarix gallica, chemical valorization, flavonoids, extraction, spectroscopy analysis, chromatography methods.

scholarly journals IDENTIFIKASI SENYAWA METABOLIT SEKUNDER DAUN PETAI CINA (Leucaena leucocephala (Lamk.) De Wit) DAN UJI AKTIVITAS ANTIBAKTERI Staphylococcus aureus

2019 ◽  
Vol 4 (1) ◽  
pp. 232-239
Author(s):  
Ermi Abriyani ◽  
Neneng Nurfalah
Keyword(s):  
Staphylococcus Aureus ◽  
Secondary Metabolites ◽  
Leucaena Leucocephala ◽  
Liquid Extraction ◽  
Diffusion Method ◽  
Extraction Column ◽  
Disk Diffusion Method ◽  
Antibacterial Test ◽  
Liquid Liquid Extraction ◽  
Paper Disk

ABSTRAK Salah satu tumbuhan yang digunakan sebagai obat tradisional adalah petai cina (Leucaena leucocephala (Lamk.) de Wit). Secara etnobotani, masyarakat Indonesia telah memanfaatkan daun petai cina sebagai obat-obatan diantaranya sebagai obat luka dan obat bengkak. Petai cina diketahui potensial untuk dikembangkan lebih lanjut pada penyakit infeksi. Berdasarkan hal ini dilakukan penelitian mengenai identifikasi metabolit sekunder daun petai cina. Metode yang digunakan yaitu soxhlet, uji fitokimia, ekstraksi cair-cair, kromatografi kolom, uji antibakteri mengunakan metode difusi paper disk dengan konsentrasi 10% b/v, 20% b/v, 30% b/v, 40% b/v, 50%. b/v. Pada pengujian karakteristik senyawa metabolit sekunder daun petai cina memiliki potensi yaitu senyawa alkaloid, flavonoid, tanin, polifenol dan steroid. Pada pengujian antibakteri menunjukkan konsentrasi 50% zona hambat paling besar dengan hasil pengukuran 6,16 mm pada ekstrak etanol dan pada ekstak n- heksana 4,44 mm.    Kata Kunci:            Daun Petai Cina (Leucaena leucocephala (Lamk.) de Wit)), Ekstraksi cair-cair, Kromatografi lapis tipis, Kromatografi kolom, St ABSTRACK One of the plants used as traditional medicine is (Leucaena leucocephala (Lamk.) De Wit). Ethnobotany, Indonesian people have used Chinese petai leaves as medicines, including wound medicine and swollen medicine. Chinese petai is known to be potential for further development in infectious diseases. Based on this, a study was conducted on the identification of secondary metabolites of Chinese petai leaves. The method used is soxhlet, phytochemical test, liquid-liquid extraction, column chromatography, antibacterial test using paper disk diffusion method with a concentration of 10% b / v, 20% b / v, 30% b / v, 40% b / v, 50%. b / v. In testing the characteristics of secondary metabolites of Chinese petai leaves, they have the potential of alkaloid compounds, flavonoids, tannins, polyphenols and steroids. The antibacterial test for a concentration of 50% has the largest inhibition zone with results of measurements of 6.16 mm on ethanol extract and on n-hexane extract 4.44 mm. Keywords: (Leucaena leucocephala (Lamk.) De Wit)), Liquid- liquid Extraction Cromatography column, Thin Layer Cromatography, Staphylococcus aureus

scholarly journals Genomic and transcriptomic survey of an endophytic fungus Calcarisporium arbuscula NRRL 3705 and potential overview of its secondary metabolites

2020 ◽  
Author(s):  
Jintao Cheng ◽  
Fei Cao ◽  
Xinai Chen ◽  
Yongquan Li ◽  
Xuming Mao
Keyword(s):  
Natural Products ◽  
Secondary Metabolites ◽  
Gene Cluster ◽  
Secondary Metabolite ◽  
Endophytic Fungi ◽  
Endophytic Fungus ◽  
Housekeeping Genes ◽  
Gene Clusters ◽  
Biosynthetic Gene ◽  
Biosynthetic Gene Clusters

Abstract Background: Secondary metabolites as natural products from endophytic fungi are important sources of pharmaceuticals. However, there is currently little understanding of endophytic fungi at the omics levels about their potential in secondary metabolites. Calcarisporium arbuscula , an endophytic fungus from the fruit bodies of Russulaceae, produces a variety of secondary metabolites with anti-cancer, anti-nematode and antibiotic activities. A comprehensive survey of the genome and transcriptome of this endophytic fungus will help to understand its capacity to biosynthesize secondary metabolites and will lay the foundation for the development of this precious resource. Results: In this study, we reported the high-quality genome sequence of C. arbuscula NRRL 3705 based on Single Molecule Real-Time sequencing technology. The genome of this fungus is over 45 Mb in size, larger than other typical filamentous fungi, and comprises 10,001 predicted genes, encoding at least 762 secretory-proteins, 386 carbohydrate-active enzymes and 177 P450 enzymes. 398 virulence factors and 228 genes related to pathogen-host interactions were also predicted in this fungus. Moreover , 65 secondary metabolite biosynthetic gene clusters were revealed, including the gene cluster for the mycotoxin aurovertins. In addition, several gene clusters were predicted to produce mycotoxins, including aflatoxin, alternariol, destruxin, citrinin and isoflavipucine. Notably, two independent gene clusters were shown that are potentially involved in thebiosynthesis of alternariol. Furthermore, RNA-Seq assays showed that only expression of the aurovertin gene cluster is much stronger than expression of the housekeeping genes under laboratory conditions, consistent with the observation that aurovertins are the predominant metabolites. Gene expression of the remaining 64 gene clusters for compound backbone biosynthesis was all lower than expression of the housekeeping genes, which partially explained poor production of other secondary metabolites in this fungus. Conclusions : Our omics data, along with bioinformatics analysis, indicated that C. arbuscula NRRL 3705 contains a large number of biosynthetic gene clusters and has a huge potential to produce a profound number of secondary metabolites. This work also provides the basis for development of endophytic fungi as a new resource of natural products with promising biological activities. Keywords: Endophytic Fungus, Calcarisporium arbuscula , Genome, Transcriptome, Secondary Metabolite

Genome sequence of Novosphingobium malaysiense strain MUSC 273T, novel alpha-proteobacterium isolated from intertidal soil

2018 ◽  
Vol 1 (1) ◽  
Author(s):  
Hooi-Leng Ser ◽  
Wai-Fong Yin ◽  
Kok-Gan Chan ◽  
Nurul-Syakima Ab Mutalib ◽  
Learn-Han Lee
Keyword(s):  
Secondary Metabolites ◽  
Secondary Metabolite ◽  
Genome Sequence ◽  
Catalase Activity ◽  
Gene Clusters ◽  
Rrna Genes ◽  
Gram Negative ◽  
Genomic Features

Novosphingobium malaysiense strain MUSC 273T is a recently identified Gram-negative, aerobic alpha-proteobacterium. The strain was isolated from intertidal soil with strong catalase activity. The genome sequence comprises 5,027,021 bp, with 50 tRNA and 3 rRNA genes. Further analysis identified presence of secondary metabolite gene clusters within genome of MUSC 273T. Knowledge of the genomic features of the strain may allow further biotechnological exploitation, particularly for production of secondary metabolites as well as production of industrially important enzymes

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