scholarly journals The Effect of Noni Leaves Extract (Morinda citrifolia L) on Wound Healing Percentage of Traumatic Ulser in Oral Mucosa of Wistar Rats (Rattus norvegicus) by in Vivo

2017 ◽  
Vol 10 (4) ◽  
pp. 1735-1740 ◽  
Author(s):  
Sri Rezeki ◽  
Sunnati Sunnati ◽  
Nadia Vidirachmilla
Keyword(s):  
Wound Healing ◽  
Oral Mucosa ◽  
Rattus Norvegicus ◽  
Wistar Rats ◽  
Morinda Citrifolia

scholarly journals Overexpression of the Oral Mucosa-Specific microRNA-31 Promotes Skin Wound Closure

2019 ◽  
Vol 20 (15) ◽  
pp. 3679 ◽  
Author(s):  
Lin Chen ◽  
Alyne Simões ◽  
Zujian Chen ◽  
Yan Zhao ◽  
Xinming Wu ◽  
...  
Keyword(s):  
Wound Healing ◽  
Oral Mucosa ◽  
Wound Closure ◽  
Expression Patterns ◽  
Skin Wound ◽  
Skin Wound Healing ◽  
Specific Expression ◽  
Keratinocyte Proliferation

Wounds within the oral mucosa are known to heal more rapidly than skin wounds. Recent studies suggest that differences in the microRNAome profiles may underlie the exceptional healing that occurs in oral mucosa. Here, we test whether skin wound-healing can be accelerating by increasing the levels of oral mucosa-specific microRNAs. A panel of 57 differentially expressed high expresser microRNAs were identified based on our previously published miR-seq dataset of paired skin and oral mucosal wound-healing [Sci. Rep. (2019) 9:7160]. These microRNAs were further grouped into 5 clusters based on their expression patterns, and their differential expression was confirmed by TaqMan-based quantification of LCM-captured epithelial cells from the wound edges. Of these 5 clusters, Cluster IV (consisting of 8 microRNAs, including miR-31) is most intriguing due to its tissue-specific expression pattern and temporal changes during wound-healing. The in vitro functional assays show that ectopic transfection of miR-31 consistently enhanced keratinocyte proliferation and migration. In vivo, miR-31 mimic treatment led to a statistically significant acceleration of wound closure. Our results demonstrate that wound-healing can be enhanced in skin through the overexpression of microRNAs that are highly expressed in the privileged healing response of the oral mucosa.

scholarly journals PENGARUH EKSTRAK DAGING LIDAH BUAYA (Aloe Vera) TERHADAP PENYEMBUHAN ULSERASI MUKOSA MULUT PADA MALE WISTAR RATS

2015 ◽  
Vol 1 (1) ◽  
pp. 25
Author(s):  
Laila Fitrotuz Zahroh ◽  
Rahmawati Sri Praptiningsih ◽  
Moh. Baehaqi
Keyword(s):  
Oral Mucosa ◽  
Wistar Rats ◽  
Healing Process ◽  
Research Result ◽  
Aloe Vera ◽  
Control Group ◽  
Control Group Design ◽  
Significant Difference ◽  
Male Wistar Rats

Background: Oral mucosa ulceration which often occurs usually in the form of white-yellowish spot with concave surface, reddish edge and pain. Based on previous research, Aloe vera process anti-inflammation substance that could help quickening ulceration healing process. This research aims to know the effect of Aloe vera flesh extract on Male wistar rats oral mucosa ulceration in-vivo. Method: this research was quasi experimental research with the post-test only control group design using Male wistar rats as the testing animal. In the research, there were three treatment groups: The first groups which was given aquadest treatment, second groups with Aloe vera flesh extract, and third groups which was given chlorhexidine gluconate 0,2% treatment. The data collecting was based on histopathology observation concerning the increase of fibroblast quantity. Result: The research result based on comparison test among the three groups with One Way Anova showed that on Day 3th, the average quantity of fibroblast didn't have significant difference between the treatment group and control group positive that was p>0,05, meanwhile on Day 7th every group showed significant difference p<0,05. Conclusion: It concluded that Aloe vera flesh extract has influence on the healing of Male wistar rats oral mucosa ulceration as shown by fibroblast increasing quantity.

Preparation and In Vivo Study of an Anti-Infective Highly Volatile Wound Dressing Material

2010 ◽  
Vol 123-125 ◽  
pp. 355-358
Author(s):  
Mubarak A. Khan ◽  
Jahid M.M. Islam ◽  
Md. Arifur Rahman ◽  
H.U. Shekhar
Keyword(s):  
Wound Healing ◽  
Rattus Norvegicus ◽  
Wound Dressing ◽  
Ventral Side ◽  
Gelatin Solution ◽  
Spray System ◽  
High Volatility ◽  
Healing Wounds ◽  
Antibiotic Dosing

In this research, a highly volatile membrane forming solution of gelatin with quick membrane forming property (30 sec) was prepared to investigate the suitability of solution spray system in the treatment of wound at dermal surface. A nontoxic Aromatic Ketone (AK) and a highly volatile solvent (VS) were introduced in gelatin solution to ensure high volatility. The solution was subjected to antibiotic dosing using Ciprofloxacin to ensure its anti-infectiveness. The pH of the raw gelatin solution and the highly volatile gelatin solution were 4.95 and 6.17 respectively. Viscosity of gelatin/AK/VS solution was 62.12mPa.s. Wound healing characteristic of the highly volatile solution (gelatin/AK/VS) was evaluated using a rat (Rattus norvegicus) model. Full-thickness wounds were created on the ventral side of the Rattus norvegicus and were dressed with the volatile solution and “eco-plaster” (control). Wound healing and bioadhesion were monitored in 3-days interval. The results revealed that prepared volatile solution was more effective in preventing blood loss as well as healing wounds than the conventional wound dresser.

scholarly journals EFEKTIVITAS PEMBERIAN EKSTRAK JAHE MERAH (ZINGIBER OFFICINALE) TERHADAP JUMLAH SEL FIBROBLAS DALAM PROSES PENYEMBUHAN ULKUS PADA MUKOSA MULUT TIKUS PUTIH JANTAN (RATTUS NORVEGICUS)

2016 ◽  
Vol 3 (2) ◽  
pp. 98
Author(s):  
Prima Agusmawanti
Keyword(s):  
Wound Healing ◽  
Oral Mucosa ◽  
Rattus Norvegicus ◽  
Healing Process ◽  
White Male ◽  
Zingiber Officinale ◽  
Male Rats ◽  
Wound Healing Process ◽  
P Value ◽  
Red Ginger

Background: In the wound healing process there are a variety of processes, including the most important are fibroplasia process that has a very important role in the wound healing process. Red ginger (Zingiber officinale) contains active ingredients include a mixture of components of essential oils and resins that oleoresin which has anti-inflammatory effects. The aim is determine the effect of red ginger extract against the number of fibroblasts in healing ulcers on the oral mucosa white male rats (Rattus norvegicus)Method: Samples using 24 male rats 2-3 months of age and body weight of 120-200 grams. Mandibular labial mucosa injured mice using H2O2 solution for 2x1 days. The samples were divided into 4 groups, where each group each of 4individuals: group 1 without any treatment, group 2 was treated application ginger gel extract 4%, group 3 was given treatment application ginger gel extract 8% and group 4 treated with applications aloclair gel. On the fourth day after the injury,the rats were decapitated, then do making preparations for histology to count the number of fibroblast cells.Result: The results Oneway ANOVA p value <0.05 and post Hoc Test LSD p value <0.05 which means that the extract of red ginger significantly affects the number of fibroblast cells and between groups have significant differencesConclusion: Extract red ginger gel is effective against the number of fibroblasts in healing ulcers on the oral mucosa white male rats.

scholarly journals Acceleration of post-tooth extraction socket healing after continuous aerobic and anaerobic physical exercise in Wistar rats (Rattus norvegicus)

2020 ◽  
Vol 53 (4) ◽  
pp. 196
Author(s):  
Aqsa Sjuhada Oki ◽  
Moch Febi Alviansyah ◽  
Christian Khoswanto ◽  
Retno Pudji Rahayu ◽  
Muhammad Luthfi
Keyword(s):  
Wound Healing ◽  
Physical Exercise ◽  
Rattus Norvegicus ◽  
Wistar Rats ◽  
Tooth Extraction ◽  
Anaerobic Exercise ◽  
Control Group ◽  
Continuous Training ◽  
Significant Difference ◽  
Aerobic And Anaerobic

Background: Physical exercise has been proven to accelerate wound healing. Physical training itself consists of aerobic (continuous training) and anaerobic (interval training) exercise. The effectiveness of continuous physical exercise on post-tooth extraction wound healing is the focus of this study. Purpose: This study aims to investigate the differences in post-tooth extraction wound healing in Wistar rats (Rattus norvegicus) after aerobic and anaerobic exercise based on the number of fibroblasts and neovascularisation. Methods: Wistar rats were divided into three groups: the control group (K1); K2 undertook continuous aerobic exercise, swimming at 50% maximum swimming capacity (MSC) with an additional 3% bodyweight load; K3 undertook anaerobic continuous exercise, swimming at 65% MSC with a 6% load. The rats swam three times per week for six weeks. The number of fibroblasts and neovascularisation were examined three days after tooth extraction. Data was analysed using the one-way analysis of variance (ANOVA) and Least Significant Difference (LSD) tests (p<0.05). Results: There was a significant difference in the number of fibroblasts between the K2 and K3 groups. There was no significant difference between K2 and K3 in the amount of neovascularisation. Conclusion: There were differences in the number of fibroblasts but not neovascularisation after tooth extraction in Wistar rats given aerobic and anaerobic continuous training.

scholarly journals Comparative study between the effects of hyaluronic acid and acid galactan purified from eggs of the mollusk Pomacea sp in wound healing

2004 ◽  
Vol 19 (1) ◽  
pp. 13-17 ◽  
Author(s):  
Ana Katarina Menezes da Cruz ◽  
Wogelsanger Oliveira Pereira ◽  
Elizeu Antunes dos Santos ◽  
Maria Goretti Freire Carvalho ◽  
Aldo da Cunha Medeiros ◽  
...  
Keyword(s):  
Wound Healing ◽  
Small Intestine ◽  
Hyaluronic Acid ◽  
Wistar Rats ◽  
Saline Solution ◽  
Giant Cells ◽  
Ion Exchange Chromatography ◽  
Exchange Chromatography ◽  
Healing Tissue

PURPOSE: To compare the effect of hyaluronic acid (HA) and of AG on the healing of intestine wounds. METHODS: The semi-purified extract of the eggs of the mollusc was obtained by fractionation with ammonium sulfate and purification for ion-exchange chromatography. The obtained galactans were eluted in water (neutral galactan) and in 0.1 and 0.2M NaCl (acidic galactans). The in vivo study was performed with 45 "Wistar" rats, separated in three groups (n=15). Solutions containing HA 1%, GA 1% or saline solution 0,9%, was placed topically on the sutures of wounds in the small intestine of the rats. After 05, 10 and 21 days the animals were sacrificed and biopsy of the healing tissue was done. RESULTS: The hystologic grading was more significant for HA and AG groups when compared to the group C. AG stimulated the appearance of macrophages, giant cells and increase in the concentration of collagen in the area of the wound when compared to HA. CONCLUSION: The topical use of GA in intestinal wounds promoted the anticipation of events that are important in the wound healing.

Effect of Topical Rambutan Honey Pharmaceutical Grade on Oral Mucosa Wound Healing Based on Tissue Wound Closure and Fibroblasts Proliferation in vivo

2015 ◽  
Vol 11 (7) ◽  
pp. 864-869
Author(s):  
E.R. Yuslianti ◽  
B.M. Bachtiar ◽  
D.F. Suniarti ◽  
A.B. Sutjiatmo ◽  
Euis Reni Yuslianti ◽  
...  
Keyword(s):  
Wound Healing ◽  
Oral Mucosa ◽  
Wound Closure

scholarly journals WOUND HEALING EFFECT OF KING ALFERD’S MUSHROOM (DALDINIA CONCENTRICA) USED BY TRIBES OF SIRUMALAI HILLS, TAMILNADU, INDIA

2017 ◽  
Vol 9 (7) ◽  
pp. 161 ◽  
Author(s):  
Rajeshwaran Thangaraj ◽  
Sumathy Raj ◽  
Kumuthakalavalli Renganathan
Keyword(s):  
Wound Healing ◽  
Wistar Rats ◽  
Field Trips ◽  
Traditional Use ◽  
Wound Healing Effect ◽  
Phytochemical Constituents ◽  
Daldinia Concentrica ◽  
Wound Healing Activity ◽  
Mushroom Sample

Objective: The present investigation is an attempt to an ethnomycological mushroom survey carried out among the paliyar tribals of five settlements in Sirumalai hills. Daldinia concentrica, mushroom reported by the tribals for the wound healing activity was evaluated in vivo in rat model. Methods: The indigenous information of the tribal people was collected through personal interviews during field trips and also the ethno reported mushroom Daldinia concetrica was analysed for its phytochemical constituents by Harborne method and its potential wound healing activity in Albino Wistar rats by excision wound model.Results: The exploration revealed that 92% of the respondents consume mushroom as food and four mushrooms are used in ethnomedicine in the study area namely, Daldinia concentrica, Calvatia gigantean, Termitomyces microcarpus and Podaxis pistillaris. Daldinia concentrica for wound healing, is the first ethnomycological report, thus the further study on Daldinia revealed that the mushroom sample consists of alkaloid 0.97±0.07 mg/kg, flavanoid 2.97±0.12 mg/kg, phenol 0.32±0.01 mg/kg, tannin 0.17±0.02 mg/kg, terpenoid 0.07±0.01 mg/kg and saponin 0.09±0.02 mg/kg; and it’s in vivo wound healing activity was found to be outstanding around 75–87% compared to that of the commercial ointment neosporin.Conclusion: We believe that wound healing activity of Daldinia mushroom was due to the synergestic effect of phytochemicals present in them. Our results throw in the insight of the traditional use of mushrooms in various treatments.

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