scholarly journals Evaluation of Concentration Dependent Antimicrobial Efficacy of Herbal And Non Herbal Dentifrices against Salivary Microflora – An In vitro Study

2018 ◽  
Vol 11 (2) ◽  
pp. 711-718 ◽  
Author(s):  
Aroonika. S. Bedre ◽  
Radhika Arjunkumar ◽  
Muralidharan N. P
Keyword(s):  
Saliva Sample ◽  
In Vitro Study ◽  
Antimicrobial Efficacy ◽  
Diffusion Method ◽  
Viable Option ◽  
Herbal Products ◽  
Zones Of Inhibition ◽  
Plaque Control ◽  
Agar Well Diffusion Method

This study focuses on evaluating the concentration dependent antimicrobial efficacy of herbal dentifrice (tooth paste) in comparison with a conventional non dentifrice. One non -herbal dentifrice and three herbal dentifrices were selected for this study. Saliva samples were collected from 10 healthy individuals. All toothpaste samples were diluted in saline in 25%, 50% and 100% concentrations. Their antimicrobial activity was determined by modified agar well diffusion method. Five wells were cut at equidistance in each of the nutrient agar plates. The plates were seeded with saliva sample. Dentifrice dilutions were introduced into the wells. The plates were incubated overnight and the diameter of zones of inhibition was measured. The antimicrobial efficacy was similar in herbal and non-herbal dentifrices and also in their different concentrations. We can advocate herbal dentifrices, as there is a sudden surge in the concern over using chemical and non-herbal products. Thus, comparable properties with standard pastes makes herbal pastes a viable option for plaque control.

scholarly journals Antibacterial Inhibitory Effects of Punica Granatum Gel on Cariogenic Bacteria: An in vitro Study

2017 ◽  
Vol 10 (2) ◽  
pp. 152-157 ◽  
Author(s):  
Grazielle Millo ◽  
Apa Juntavee ◽  
Ariya Ratanathongkam ◽  
Natsajee Nualkaew ◽  
Peerapattana, Jomjai ◽  
...  
Keyword(s):  
High Performance ◽  
In Vitro Study ◽  
Punica Granatum ◽  
Antibacterial Activities ◽  
Diffusion Method ◽  
Inhibitory Effects ◽  
Cariogenic Bacteria ◽  
Zones Of Inhibition ◽  
Time Kill

ABSTRACT Aim This study evaluated the in vitro antibacterial effects of the formulated Punica granatum (PG) gel against Streptococcus mutans, Streptococcus sanguinis, and Lactobacillus casei. Materials and methods The PG extract was dissolved in water at 500 mg/mL. High performance liquid chromatography (HPLC) was used for identification and quantification of chemical marker punicalagin. Minimum bactericidal concentration (MBC) and time-kill assay (TKA) were investigated. Antibacterial activities of the formulated PG gel, 2% chlorhexidine (CHX) gel and blank gel were tested by measuring the zones of inhibition through agar well diffusion method. Results The HPLC results showed presence of punicalagin at 2023.58 ± 25.29 μg/mL in the aqueous PG extract and at 0.234% (w/w) in the formulated PG gel. The MBC for S. mutans, S. Sanguinis, and L. casei were 250, 125, and 500 mg/mL respectively. The TKA of 500 mg/mL aqueous PG extract showed total inhibition of S. mutans, S. Sanguinis, and L. casei at 6, 1, and 24 hours contact time respectively. Agar well diffusion revealed that for S. mutans, CHX gel > PG gel > blank gel; for S. sanguinis, CHX gel = PG gel > blank gel; for L. casei, CHX gel > PG gel = blank gel. Comparison of the PG gel potency showed that S. sanguinis = S. mutans > L. casei. Conclusion The PG gel equivalent to 0.234% punicalagin (w/w) inhibited S. mutans and S. sanguinis but not L. casei within 24 hours incubation period and has the potential to be used for caries prevention. How to cite this article Millo G, Juntavee A, Ratanathongkam A, Nualkaew N, Peerapattana J, Chatchiwiwattana S. Antibacterial Inhibitory Effects of Punica Granatum Gel on Cariogenic Bacteria: An in vitro Study. Int J Clin Pediatr Dent 2017;10(2):152-157.

scholarly journals Antifungal activity of Ventilago maderspatana on Trichophyton rubrum - An in vitro study

2020 ◽  
Vol 5 (05) ◽  
pp. 179-185
Author(s):  
Rajalekshmi S ◽  
Beni PB ◽  
Gopikrishna S
Keyword(s):  
Antifungal Activity ◽  
Specific Treatment ◽  
In Vitro Study ◽  
Cross Sectional Study ◽  
Chloroform Extract ◽  
Diffusion Method ◽  
Cross Sectional ◽  
Dermatophyte Species ◽  
Agar Well Diffusion Method

The aim of the study was to contribute a single drug for specific dermatophyte species to encourage diagnosis specific treatment. The study had two arms one clinical part consisting of cross-sectional study of 80 participants and other part invitro study. The survey was conducted to know the prevalence of dermatophyte species among cutaneous dermatophytosis patients in Kottakkal population there by collecting the skin scrapings of the patients. As the second part of the study these skin scrapings were cultured in sabouraud dextrose agar (SDA) and the species were inoculated from their and identified. Thus from 80 participants 2-genus and 6 different species were obtained. Trichophyton and microsporum were the genus and among Trichophyton 4 species like T.rubrum, T.mentagrophytes, T.tonsurans and T.verrucossum and Microsporum genus consists of M.canis and Microsporumaudouinii. First MIC was calculated and after that antifungal activity was done through the agar well diffusion method using ventilago maderaspattana extracts against fluconazole. Three different extracts were used chloroform, methanol and ethanol. In this article the action of easily available low polarity chloroform extract against the most prevalent species T. rubrum is highlighted because it shows the highest mean zone of inhibition in agar well diffusion method.

scholarly journals Comparison of Antimicrobial Activity of Child Formula Dentifrices at different Concentrations: An in vitro Study

2017 ◽  
Vol 10 (2) ◽  
pp. 131-135
Author(s):  
Ritika Malhotra ◽  
ND Shashikiran
Keyword(s):  
Antimicrobial Activity ◽  
Minimum Inhibitory Concentration ◽  
Inhibitory Concentration ◽  
Fluoride Concentration ◽  
Agar Plate ◽  
In Vitro Study ◽  
Diffusion Method ◽  
Distilled Water ◽  
Zones Of Inhibition

ABSTRACT Aim The aim of the present in vitro study is to evaluate and compare antimicrobial efficacy of commercially available child's dental formulas in reduced concentrations containing different forms of fluoride against Streptococcus mutans activity. Materials and methods The selected dentifrices were prepared in dilutions of 1:1, 1:2, 1:4, 1:8, and 1:16 using sterile pyrogen-free distilled water. Various dilutions of the selected toothpaste slurries were incubated in the agar plate containing pure strains of S. mutans, and antimicrobial activity of each was assessed by measuring the diameter of zones of inhibition (in mm). Agar well plate diffusion method and minimum inhibitory concentration (MIC) determination were the methods used in this study. The inhibitory circle of each dentifrice was measured and MIC was achieved by considering the value of diameter of the circle. Results The results of the study showed that even at a lower concentration of fluoride, inhibition halos were obtained for all the formulations at different dilutions. Conclusion The kid's formulations having lower fluoride concentration show antimicrobial activity even after dilutions. Thus, commercially, the fluoride concentrations can be further lowered down in the dentifrices, thereby reducing the risk associated with fluoride. How to cite this article Malhotra R, Singla S, Shashikiran ND. Comparison of Antimicrobial Activity of Child Formula Dentifrices at different Concentrations: An in vitro Study. Int J Clin Pediatr Dent 2017;10(2):131-135.

scholarly journals IN VITRO STUDY OF AYURVEDIC CONDITIONER W.S.R. TO ANTIDANDRUFF ACTIVITY

2020 ◽  
Vol 8 (10) ◽  
pp. 4748-4752
Author(s):  
Jain Vatsala ◽  
Rao K. Shankar
Keyword(s):  
Antibacterial Activity ◽  
Treatment Options ◽  
Research Work ◽  
In Vitro Study ◽  
Vitro Study ◽  
Diffusion Method ◽  
Herbal Preparation ◽  
Skin Cells ◽  
Agar Well Diffusion Method

Objective: Dandruff is the unusually high shedding of dead skin cells from the scalp. It is a common scalp disorder affecting almost half of the post pubertal population. In the market many treatment options are available but herbal preparation is the best option to treat dandruff. Thus, in the present research work Ayurvedic conditioner prepared with plant extract (decoction) was studied to evaluate antidandruff activity against Staphyllococus aureus & Candida albicans. Method In vitro study was conducted to evaluate the antidandruff effect of Ayurvedic conditioner against S. aureus & C. albicans. The evaluation done by adopting Kirby-Bauer Agar Well diffusion method. Antibacterial activity was compared with the standard references. Result Study revealed that Ayurvedic conditioner was susceptible for the same microbes in less concentration. Conclusion Ayurvedic conditioner was found to be the most effective formulation against dandruff causing factors; s.aureus and c.albicans.

scholarly journals Antimicrobial Efficacy of Calcium Hydroxide and Triple Antibiotic Paste Combination on E. faecalis Biofilm- an In vitro Study

2020 ◽  
pp. 120-126
Author(s):  
Azima Hanin S. M. ◽  
Anjaneyulu K. ◽  
Muralidharan N. P.
Keyword(s):  
Calcium Hydroxide ◽  
In Vitro Study ◽  
Antimicrobial Efficacy ◽  
Diffusion Method ◽  
P Value ◽  
Tryptone Soya Agar ◽  
Triple Antibiotic Paste ◽  
High Alkalinity ◽  
Post Hoc

Calcium hydroxide (CH) is an intracanal medicament that has been widely used in endodontics, which can eliminate bacteria because of its high alkalinity. However, E. faecalis is resistant to CH. Triple antibiotic paste (TAP) is a mixture of ciprofloxacin, minocycline, and metronidazole, and is highly effective against E. faecalis. Hence the main aim of this study was to find the antimicrobial efficacy of CH and TAP combination against E. faecalis. The study was done by agar diffusion method, three wells were punched in Tryptone soya agar and filled with CH, TAP, and the combination of both. The zone of inhibition values was recorded and subjected to statistical analysis using SPSS. One way ANOVA and Post Hoc tests were used to compare the means. The combination of CH with TAP was found to be significantly better than CH and TAP used alone (p-value <0.05).Within the limits of this study, it can be concluded that TAP is more efficient when compared with CH,The combination of CH and TAP proved to be more effective when compared to the two when used alone.

scholarly journals Analysis of Antimicrobial Efficacy of Triphala and its Effectiveness in Decontaminating the Guttapercha Cones

2018 ◽  
Vol 14 (4) ◽  
pp. 217-220
Author(s):  
Manu Rana ◽  
Shiksha Kumari ◽  
Manoj Kumar Upadhyay
Keyword(s):  
Hydrogen Peroxide ◽  
Agar Plate ◽  
Endodontic Treatment ◽  
Antimicrobial Efficacy ◽  
Diffusion Method ◽  
Staph Aureus ◽  
Zones Of Inhibition ◽  
Aseptic Conditions ◽  
Prime Target ◽  
Agar Well Diffusion Method

Background: -The prime target of endodontic treatment is to maintain an aseptic conditions starting right from the access opening to the obturationof root canals.Many chemicals such as, hydrogen peroxide, chlorhexidineetc have been tried for GP decontamination but none of these compounds have been proven as fully effective.The purpose of this study was to evaluate the effectiveness of an ayurvedic alternative, Triphala for rapid decontamination of guttapercha cones. Materials and Methods: A concentrated extract of triphala was used to check for the antimicrobial efficacy using the agar well diffusion method. Presence of zones’ of diffusion was identified against three common GP contaminants namely, E.coli, E.faecalis and Staph. aureus. New GP Cones, freshly taken out of the packet were then decontaminated for 1minute using triphalaand then placed in thioglycolate broth to check for the presence of turbidity.Results: The zones of inhibition on the agar plate were measured as 23mm, 21mm and 24mm respectively. The broth remained clear even after 48 hours of incubation.Conclusion: We conclude that triphala is indeed effective as a GP decontaminant and it holds promising future as a medium for storage of GP cones. Keywords: decontamination; guttapercha cones; triphala.

QUALITY CONTROL AND IN-VITRO STUDY RELEASE BEHAVIOUR OF EURYCOMANONE IN HERBAL PRODUCTS SUPPLEMENT IN CAPSULE FORM

2020 ◽  
Vol 24 (04) ◽  
pp. 640-649
Author(s):  
Noorazwani Zainol ◽  
Mohamad Shahrizad Pairon ◽  
Mohd Eeyad Arief Mohd Nor Asri ◽  
Khetiswari Ganesan
Keyword(s):  
Quality Control ◽  
In Vitro Study ◽  
Vitro Study ◽  
Herbal Products

scholarly journals Evaluation of Antimicrobial Efficacy and Permeability of Various Sealing Materials at the Implant–Abutment Interface—A Pilot In Vitro Study

Materials ◽  
2021 ◽  
Vol 14 (2) ◽  
pp. 385
Author(s):  
Igor Smojver ◽  
Marko Vuletić ◽  
Dražena Gerbl ◽  
Ana Budimir ◽  
Mato Sušić ◽  
...  
Keyword(s):  
Fungal Infections ◽  
Aerobic Condition ◽  
In Vitro Study ◽  
Vitro Study ◽  
Antimicrobial Efficacy ◽  
Sealing Material ◽  
Implant Abutment ◽  
Static Conditions ◽  
Sealing Materials

The microenvironment of the oral cavity is altered when an implant, a biocompatible foreign body, is inserted into the mouth. Bacteria settle in the tissues in and around the implant due to the passage of microorganisms through the microgap at the connection of the implant and prosthetic abutment. To prevent colonization of the implant by microorganisms, one idea is to use sealing and antimicrobial materials to decontaminate the implant–abutment interface and close the microgap. The purpose of this study is to evaluate the antimicrobial efficacy and permeability of different types of sealing materials at the implant–abutment interface, under static conditions. Three different sealing material (GapSeal gel, Oxysafe gel and Flow.sil) were used for sealing the implant–abutment interfaces in 60 titanium dental implants, which were first contaminated with a solution containing Staphylococcus aureus and Candida albicans for 14 days under an aerobic condition. Results showed that a complete seal against bacterial infection was not formed at the implant–abutment interface, while for fungal infections, only GapSeal material helped to prevent microleakage. Findings of this in vitro study reported that application of sealing material before abutment connection may reduce peri-implant bacterial and fungal population compared with the interface without sealing material.

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