Synthesis of Silver Nanoparticles by Phyllanthus emblica Plant Extract and Their Antibacterial Activity

The silver nanoparticle was successfully synthesized by using the help of Phyllanthus emblica plant extract as a reducing agent and aqueous silver nitrate as the precursor. Moreover, physical and chemical methods are widely used for the synthesis of nanoparticles, but these methods have expensive and not ecofriendly. This study highlights the green, rapid, facile, cost-effective, and ecofriendly synthesis and synthesized nanoparticles also investigate their antibacterial activity. Synthesized silver nanoparticles are analyzed by different techniques of modes like XRD, UV-Visible spectroscopy, TEM, FTIR, and photoluminescence (PL). The prepared AgNPs show characteristic absorption peak in UV-Visible spectroscopy due to SPR (surface plasmonic resonance) band between 400 to 450 nm wavelength, which was confirmed by TEM (transmission electron microscopy) image. X-ray diffraction (XRD) results showed the crystalline nature of AgNPs as well as the size of nanoparticles calculated with the help of TEM (20-25 nm) and XRD (25 nm). ATR spectroscopy identified the functional groups that are involved in the reduction of silver ion to AgNPs and the PL spectrum indicates higher emission in the green region and low emission peak in the UV region. Antibacterial activity of AgNPs analyzed against with the help of E.Coli bacteria and the result shows that a higher concentration of AgNPs is increasing as well as a zone of inhibition increased. This method is environmentally friendly, of low cost, and less expensive method for the fabrication of AgNPs in abundance which can be further helpful for biosensor devices as well as for other applications such as pollutant degradation, pharmaceutical, and hydrogen production, etc therefore can promote the application of green technology for the production of AgNPs.

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Application of Acacia modesta and Dalbergia sissoo gums as green matrix for silver nanoparticle binding

Green Processing and Synthesis ◽

10.1515/gps-2015-0064 ◽

2016 ◽

Vol 5 (1) ◽

Cited By ~ 1

Author(s):

Hira Munir ◽

Muhammad Shahid ◽

Fozia Anjum ◽

Muhammad Nadeem Akhtar ◽

Sayed M. Badawy ◽

...

Keyword(s):

Silver Nanoparticles ◽

Antibacterial Activity ◽

Low Cost ◽

Dalbergia Sissoo ◽

Visible Spectroscopy ◽

Green Matrix ◽

Gram Negative ◽

Gram Positive Bacteria ◽

Uv Visible ◽

Cost Efficient

AbstractA low-cost, efficient, and ecofriendly method for the synthesis of silver nanoparticles (AgNPs) using gums as reducing agent was performed. The obtained nanoparticles were characterized by UV-visible spectroscopy. The antibacterial activity of the prepared nanoparticles was tested against Gram-negative and Gram-positive bacteria. The

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Phyto-Extract-Mediated Synthesis of Silver Nanoparticles Using Aqueous Extract of Sanvitalia procumbens, and Characterization, Optimization and Photocatalytic Degradation of Azo Dyes Orange G and Direct Blue-15

Molecules ◽

10.3390/molecules26206144 ◽

2021 ◽

Vol 26 (20) ◽

pp. 6144

Author(s):

Madeeha Aslam ◽

Fozia Fozia ◽

Anadil Gul ◽

Ijaz Ahmad ◽

Riaz Ullah ◽

...

Keyword(s):

Silver Nanoparticles ◽

Carboxylic Acid ◽

Plant Extract ◽

Visible Spectroscopy ◽

X Ray ◽

Direct Blue ◽

Orange G ◽

Ft Ir ◽

Uv Visible ◽

Direct Blue 15

Green synthesis of silver nanoparticles (AgNPs) employing an aqueous plant extract has emerged as a viable eco-friendly method. The aim of the study was to synthesize AgNPs by using plant extract of Sanvitalia procumbens (creeping zinnia) in which the phytochemicals present in plant extract act as a stabilizing and reducing agent. For the stability of the synthesized AgNPs, different parameters like AgNO3 concentration, volume ratios of AgNO3, temperature, pH, and contact time were studied. Further, AgNPs were characterized by UV–visible spectroscopy, FT-IR (Fourier Transform Infrared Spectroscopy), XRD (X-ray Diffraction), SEM (Scanning Electron Microscopy), and EDX (Energy Dispersive X-ray Spectrometer) analysis. FT-IR analysis showed that the plant extract contained essential functional groups like O–H stretching of carboxylic acid, N–H stretching of secondary amides, and C–N stretching of aromatic amines, and C–O indicates the vibration of alcohol, ester, and carboxylic acid that facilitated in the green synthesis of AgNPs. The crystalline nature of synthesized AgNPs was confirmed by XRD, while the elemental composition of AgNPs was detected by energy dispersive X-ray analysis (EDX). SEM studies showed the mean particle diameter of silver nanoparticles. The synthesized AgNPs were used for photocatalytic degradation of Orange G and Direct blue-15 (OG and DB-15), which were analyzed by UV-visible spectroscopy. Maximum degradation percentage of OG and DB-15 azo dyes was observed, without any significant silver leaching, thereby signifying notable photocatalytic properties of AgNPs.

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GREEN SYNTHESIS, CHARACTERIZATION OF SILVER NANOPARTICLES OF A MARINE RED ALGA SPYRIDIA FUSIFORMIS AND THEIR ANTIBACTERIAL ACTIVITY

International Journal of Pharmacy and Pharmaceutical Sciences ◽

10.22159/ijpps.2017v9i5.17105 ◽

2017 ◽

Vol 9 (5) ◽

pp. 192 ◽

Cited By ~ 7

Author(s):

Subbiah Murugesan ◽

Sundaresan Bhuvaneswari ◽

Vajiravelu Sivamurugan

Keyword(s):

Silver Nanoparticles ◽

Antibacterial Activity ◽

Green Synthesis ◽

Red Alga ◽

Diffusion Method ◽

Present System ◽

X Ray Diffraction ◽

Visible Spectroscopy ◽

X Ray ◽

Uv Visible

Objective: In the present system, the green synthesis of silver nanoparticles using marine the red alga Spyridia fusiformis and antibacterial activity was carried out.Methods: The seaweed extract was used for the synthesis of AgNPs at room temperature. The silver nanoparticles were characterized by using UV–Visible spectroscopy, Fourier transform infrared spectroscopy, transmission electron microscope and X-ray diffraction (XRD) techniques. The antibacterial activity of biosynthesized silver nanoparticles was carried out by disc diffusion method against pathogenic bacteria.Results: The UV-visible spectroscopy revealed surface plasmon resonance at 450 nm. The FT-IR measurements showed the possible functional groups responsible for the formation of nanoparticles. The X-ray diffraction analysis showed that the particles were crystalline in nature. TEM micrograph has shown the formation of silver nanoparticles with the size in the range of 5–50 nm. The silver nanoparticles synthesized from the S. fusiformis showed higher activity and proved their efficacy in controlling the pathogenic bacterial strains. The nanoparticles showed highest inhibition activity on K. pneumaniae and S. aureus up to 26 and 24±0.01 mm at 100 μg/ml of nanoparticles.Conclusion: The synthesised AgNPs have shown the best antibacterial activity against human pathogens E. coli, K. pneumoniae, S. aureus and P. aeruginosa. The above eco-friendly AgNPs synthesis procedure could be a viable solution for industrial applications in the future and therapeutic needs.

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Fungus (Alternaria sp.) Mediated Silver Nanoparticles Synthesis, Characterization and Application as Phyto-Pathogens Growth Inhibitor

10.21203/rs.3.rs-72789/v1 ◽

2020 ◽

Author(s):

Theint Theint Win ◽

Sikandar Khan ◽

Pengcheng Fu

Keyword(s):

Silver Nanoparticles ◽

Low Cost ◽

Growth Inhibitor ◽

Pathogenic Fungi ◽

Inhibition Zone ◽

Phytopathogenic Fungi ◽

Plant Diseases ◽

Synthesis Of Nanoparticles ◽

Alternaria Sp ◽

Uv Visible

Abstract Background: Biogenic nanoparticles have proved to be effective biocontrol agents for certain plant diseases. It possesses the potential for extensive use for sustainable agriculture. Many attempts have been made to synthesize nano-based antifungal compounds for the management of soil borne pathogenic fungi for crops.Results: In our work, silver nanoparticles (AgNPs) was constructed with phytopathogenic fungi (Alternaria sp.) which was isolated from banana cultivated soil. Alternaria sp. was able to grow rapidly and produce highly bioactive compounds as safe antifungal agent against plant pathogenic fungi (Fusarium spp. and Alternaria sp.). The size of synthesized silver nanoparticles ranged between 5-10 nm. Analytic tools, such as UV-visible spectroscopy, Fourier transformed infra-red (FTIR) spectroscopy, scanning transmission electron microscopy (STEM), EDS and elemental mapping were used to visualize the formation of AgNPs. The UV-visible spectra showed the peak at 435 nm. The maximum inhibition zone was observed at 100 µl concentration of AgNPs for Fusarium oxysporum (21 ± 2 mm) following Alternaria sp. (20± 2 mm), suggested that the efficacy of the biosynthesized NPs against the phytopathogenic fungi.Conclusions: The resulting AgNPs showed distinct antifungal activity against selected pathogenic plant fungi. The work indicates that green reduction and biogenic synthesis of nanoparticles with benign fungi is an effective, low cost, sustainable and environmentally friendly approach for prevention of soil borne plant diseases.

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Green Synthesis of Silver Nanoparticles from several NTFP Plants

Notulae Scientia Biologicae ◽

10.15835/nsb819742 ◽

2016 ◽

Vol 8 (1) ◽

pp. 106-111

Author(s):

Somnath BHOWMIK ◽

Badal Kumar DATTA ◽

Ajay Krishna SAHA ◽

Pradyut CHAKMA ◽

Narayan Chandra MANDAL

Keyword(s):

Silver Nanoparticles ◽

Plant Extract ◽

Silver Ions ◽

Biological Synthesis ◽

Synthesis Of Nanoparticles ◽

Force Microscopy ◽

Atomic Force ◽

One Step ◽

Aqueous Leaf Extract ◽

Uv Visible

The biological synthesis of nanoparticles using plant extracts plays an important role in the field of nanotechnology. In this study, rapid, simple approach was applied for synthesis of silver nanoparticles using , Clerodendrum infortunatum, Mucuna interrupta, Phlogancanthus thyrsiflorus and Sansevieria trifasciata aqueous leaf extract. The plant extract acts both as reducing agent as well as capping agent. To identify the compounds responsible for reduction of silver ions, the functional groups present in plant extract were investigated by FTIR. Various techniques used to characterize synthesized nanoparticles are Scanning Electron Microscopy (SEM), Atomic Force Microscopy (AFM) and UV–Visible spectrophotometer. Results confirmed that this protocol was simple, rapid, one step, eco-friendly, non-toxic and might be an alternative conventional physical/chemical methods. Conversion of silver nanoparticles takes place at room temperature without the involvement of any hazardous chemicals.

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SYNTHESIS OF SILVER NANOPARTICLES FROM THE MEDICINAL PLANT BAUHINIA ACUMINATA AND BIOPHYTUM SENSITIVUM–A COMPARATIVE STUDY OF ITS BIOLOGICAL ACTIVITIES WITH PLANT EXTRACT

International Journal of Applied Pharmaceutics ◽

10.22159/ijap.2017v9i1.16277 ◽

2016 ◽

Vol 9 (1) ◽

pp. 22 ◽

Cited By ~ 3

Author(s):

Elizabath Antony ◽

Mythili Sathiavelu ◽

Sathiavelu Arunachalam

Keyword(s):

Silver Nanoparticles ◽

Plant Extract ◽

Silver Nanoparticle ◽

Plant Extracts ◽

Biological Activities ◽

Alpha Tocopherol ◽

Visible Spectroscopy ◽

Leaf Extracts ◽

Uv Visible ◽

Dpph Scavenging

Objective: The aim of current study was to synthesise silver nanoparticles from the leaf extracts (aqueous and methanol) of two medicinal plants Bauhinia acuminata and Biophytum sensitivum and to compare its biological activities with that of plant extract.Methods: Silver nanoparticles were synthesised, and it was characterised using UV-Visible spectroscopy and scanning electron microscope (SEM). Fourier transform infrared spectroscopy (FTIR) and GC-MS analysis were done for silver nanoparticle extract. The biological activities such as DPPH scavenging assay, haemolytic assay and antimicrobial assay were done for both nanoparticle and plant extract.Results: The UV-Visible spectroscopy showed the formation of nanoparticles in a size range of 400-460 nm. GC-MS analysis showed the presence of biologically active compounds like DL-alpha-tocopherol and Alpha-tocopherol-beta-D-mannose. FTIR analysis of silver nanoparticles and leaf extracts showed the formation of aldehydes, alkenes, amines, alcohols, etc., which confirmed the presence of the compounds present in plant extracts. SEM image showed the formation of nanoparticles of size 2 micrometre. Phytochemical analysis of plant extracts showed the presence of carbohydrates, phenols, flavonoids, saponins, tannins and terpenoids. The methanol extract of Bauhinia acuminata showed high DPPH scavenging activity of 90% compared to that of the silver nanoparticle. The percentage hemolysis of all extracts was found to be 6%-39%. The antimicrobial activity of leaf extracts showed excellent activity towards Bacillus cereus and Listeria monocytogens.Conclusion: The results of present study showed that the silver nanoparticle synthesised from the plant extract has many bioactive compounds and it was found to have significant biological activities but comparatively lesser than plant extract. It concludes the both plant and nanoparticle extract can be used as a potential resource for therapeutic purpose.

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Role of Synthetic Plant Extracts on the Production of Silver-Derived Nanoparticles

Plants ◽

10.3390/plants10081671 ◽

2021 ◽

Vol 10 (8) ◽

pp. 1671

Author(s):

Sabah Alzharani ◽

Sergio Astudillo-Calderón ◽

Beatriz Pintos ◽

Elena Pérez-Urria ◽

José Antonio Manzanera ◽

...

Keyword(s):

Silver Nanoparticles ◽

Gallic Acid ◽

Plant Extracts ◽

Nanoparticle Synthesis ◽

Visible Absorption ◽

Synthesis Of Nanoparticles ◽

Resonance Band ◽

Uv Visible ◽

Β Carotene

The main antioxidants present in plant extracts—quercetin, β-carotene, gallic acid, ascorbic acid, hydroxybenzoic acid, caffeic acid, catechin and scopoletin—are able to synthesize silver nanoparticles when reacting with a Ag NO3 solution. The UV-visible absorption spectrum recorded with most of the antioxidants shows the characteristic surface plasmon resonance band of silver nanoparticles. Nanoparticles synthesised with ascorbic, hydroxybenzoic, caffeic, and gallic acids and scopoletin are spherical. Nanoparticles synthesised with quercetin are grouped together to form micellar structures. Nanoparticles synthesised by β-carotene, were triangular and polyhedral forms with truncated corners. Pentagonal nanoparticles were synthesized with catechin. We used Fourier-transform infrared spectroscopy to check that the biomolecules coat the synthesised silver nanoparticles. X-ray powder diffractograms showed the presence of silver, AgO, Ag2O, Ag3O4 and Ag2O3. Rod-like structures were obtained with quercetin and gallic acid and cookie-like structures in the nanoparticles obtained with scopoletin, as a consequence of their reactivity with cyanide. This analysis explained the role played by the various agents responsible for the bio-reduction triggered by nanoparticle synthesis in their shape, size and activity. This will facilitate targeted synthesis and the application of biotechnological techniques to optimise the green synthesis of nanoparticles.

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Green Synthesis of Silver Nanoparticles by Allamanda cathartica L. Leaf Extract and Evaluation for Antimicrobial Activity

International Journal of Pharmaceutical Sciences and Nanotechnology ◽

10.37285/ijpsn.2013.6.4.9 ◽

2013 ◽

Vol 6 (4) ◽

pp. 2260-2268

Author(s):

M. Linga Rao ◽

Bhumi G ◽

Savithramma N

Keyword(s):

Antimicrobial Activity ◽

Silver Nanoparticles ◽

Leaf Extract ◽

Fusarium Species ◽

Inhibition Zone ◽

Fungal Species ◽

Green Technology ◽

Metallic Silver ◽

Synthesis Of Nanoparticles ◽

Aqueous Leaf Extract

Silver nanoparticles (SNPs) exhibit tremendous applications in medicine as antimicrobial agent. The use of different parts of plants for the synthesis of nanoparticles is considered as a green technology as it does not involve any harmful chemicals. In the present study, we report a rapid biosynthesis of silver nanoparticles from aqueous leaf extract of medicinal plant Allamanda cathartica. The active phytochemicals present in the plant were responsible for the quick reduction of silver ion to metallic silver nanoparticles. The reduced silver nanoparticles were characterized by using UV-Vis spectrophotometry, Scanning Electron Microscope (SEM), Energy Dispersive Analysis of X-ray (EDAX) and Atomic Force Microscopy (AFM). The spherical shaped silver nanoparticles were observed and it was found to 19-40 nm range of size. These phytosynthesized SNPs were tested for their antimicrobial activity and it analyzed by measuring the inhibitory zone. A. cathartica aqueous leaf extract of SNPs showed highest toxicity to Pseudomonas followed by Klebsiella, Bacillus and E. coli and lowest toxicity towards Proteus. In fungal species, highest inhibition zone was noted against Rhizopus followed by Curvularia, Aspergillus flavus and Aspergillus niger and minimum inhibition zone was observed against Fusarium species. These results suggest a promising potential of Indian plant-based green chemistry for production of SNPs for biomedical and nanotechnology applications.

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Green synthesis of silk sericin-embedded silver nanoparticles and their antibacterial application against multidrug-resistant pathogens

Journal of Genetic Engineering and Biotechnology ◽

10.1186/s43141-021-00176-5 ◽

2021 ◽

Vol 19 (1) ◽

Author(s):

Md. Abdullah Al Masud ◽

Hamid Shaikh ◽

Md. Shamsul Alam ◽

M. Minnatul Karim ◽

M. Abdul Momin ◽

...

Keyword(s):

Silver Nanoparticles ◽

Green Synthesis ◽

Bombyx Mori ◽

Multidrug Resistant ◽

Visible Spectroscopy ◽

Silk Sericin ◽

X Ray ◽

Synthesis Strategy ◽

Uv Visible ◽

Multidrug Resistant Pathogens

Abstract Background The green synthesis strategy of metallic nanoparticles (NPs) has become popular due to being environmentally friendly. Stable silver nanoparticles (AgNPs) have been synthesized by natural products such as starch, soy protein, various extract of leaves, barks, and roots functioning both as reducing and stabilizing agents. Likewise, silk sericin (SS) is a globular protein discarded in the silk factory might be used for NP synthesis. In this research, we focus on the green synthesis and stabilization of AgNPs by SS as well as assessment of their antibacterial activities against some drug-resistant pathogen. Results SS was extracted from Bombyx mori silkworm cocoons in an aqueous medium. 17 w/w% of dry sericin powder with respect to the cocoon’s weight was obtained by freeze-drying. Furthermore, AgNPs conjugated to sericin, i.e., SS-capped silver nanoparticles (SS-AgNPs) were synthesized by easy, cost-effective, and environment-friendly methods. The synthesized SS-AgNPs were characterized by UV-visible spectroscopy, Fourier-transform infrared-attenuated total reflection (FTIR-ATR) spectroscopy, transmission electron microscopy (TEM), and X-ray diffraction measurement. It has been found from the absorbance of UV-visible spectroscopy that a higher percent of SS-AgNPs was obtained at a higher concentration of silver nitrate solution. FTIR-ATR spectra showed that the carboxylate groups obtained from silk sericin act as a reducing agent for the synthesis of silver nanoparticles, while NH2+ and COO− act as a stabilizer of AgNPs. The X-ray diffractogram of SS-AgNPs was quite different from AgNO3 and sericin due to a change in the crystal structure. The diameter of AgNPs was around 20–70 nm observed using TEM. The synthesized SS-AgNPs exhibited strong antibacterial activity against multidrug-resistant pathogens, Escherichia coli and Pseudomonas aeruginosa. Minimal inhibitory/bactericidal concentrations against E. coli and P. aeruginosa were 20μg/mL. Conclusions This study encourages the use of Bombyx mori for the ecofriendly synthesis of SS-AgNPs to control multidrug-resistant microorganisms.

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Antibacterial Activity of Silver Nanoparticles Synthesized by Bark Extract of Syzygium cumini

Journal of Nanoparticles ◽

10.1155/2013/431218 ◽

2013 ◽

Vol 2013 ◽

pp. 1-6 ◽

Cited By ~ 68

Author(s):

Ram Prasad ◽

Vyshnava Satyanarayana Swamy

Keyword(s):

Silver Nanoparticles ◽

Antibacterial Activity ◽

Cost Effective ◽

Diffusion Method ◽

Bark Extract ◽

Force Microscopy ◽

Atomic Force ◽

Cost Effective Method ◽

Uv Visible ◽

Major Attention

The unique property of the silver nanoparticles having the antimicrobial activity drags the major attention towards the present nanotechnology. The environmentally nontoxic, ecofriendly, and cost-effective method that has been developed for the synthesis of silver nanoparticles using plant extracts creates the major research interest in the field of nanobiotechnology. The synthesized silver nanoparticles have been characterized by the UV-visible spectroscopy, atomic force microscopy (AFM), and scanning electron microscopy (SEM). Further, the antibacterial activity of silver nanoparticles was evaluated by well diffusion method, and it was found that the biogenic silver nanoparticles have antibacterial activity against Escherichia coli (ATCC 25922), Staphylococcus aureus (ATCC 29213), Pseudomonas aeruginosa (ATCC 27853), Azotobacter chroococcum WR 9, and Bacillus licheniformis (MTCC 9555).

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